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Cheng L.,Central China Normal University | Sun Y.H.,Wuhan Aquaculture Science Research Institute | Sun Y.H.,Wuhan Academy of Agricultural Science Technology
Genetics and Molecular Research | Year: 2015

Myostatin (MSTN) is a member of the transforming growth factor-β superfamily that negatively regulates skeletal muscle development and growth. In the present study, partial genomic fragments of MSTN were screened for single nucleotide polymorphisms (SNPs) in a hybrid of Culter alburnus (♀) x Ancherythroculter nigrocauda (♂) individuals from a commercial hatchery population, and two non-synonymous SNPs (c.6T>C and c.162G>A) and two synonymous SNPs (c.152G>A and c.155G>A) in exon 2 were identified. The two non-synonymous SNPs caused an amino acid change, from Ser to Pro and from Val to Ile, respectively. Genotyping by the direct sequencing of polymerase chain reaction products for these four SNPs was conducted in 190 individuals from the commercial hatchery population. Association analysis showed that one non-synonymous SNP (c.6T>C) in exon 2 was significantly associated with total length, body length, body height, head length, and body weight. Haplotype analyses re vealed that the haplotype combination H1H3 exhibited the best growth performance. Our results demonstrate that some of the SNPs in MSTN may have positive effects on growth, and suggest that MSTN could be a candidate gene for marker-assisted selection in C. alburnus and A. nigrocauda. ©FUNPEC-RP. Source


Sun Y.,Wuhan Aquaculture Science Research Institute | Sun Y.,Wuhan Academy of Agricultural Science Technology | Sun Y.,Wuhan Xianfeng Aquaculture Technology Co. | Wang G.,Wuhan Aquaculture Science Research Institute | And 9 more authors.
Conservation Genetics Resources | Year: 2014

Twelve polymorphic microsatellite markers from Ancherythoculter nigrocauda were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. These loci were characterized by genotyping 48 individuals. The observed number of alleles per locus ranged from 4 to 14 with an average of 9.4. The expected and observed heterozygosities ranged from 0.185 to 0.907 and from 0.083 to 0.952, respectively. Among these polymorphic microsatellite loci, four of the loci (HWB01, HWB04, HWB12 and HWB16) significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction (P < 0.05). All of the microsatellite loci were in linkage equilibrium. These microsatellite markers would be useful for investigating the genetic diversity and population structure of A. nigrocauda. © 2014, Springer Science+Business Media Dordrecht. Source


Sun Y.,Wuhan Aquaculture Science Research Institute | Sun Y.,Wuhan Academy of Agricultural Science Technology | Li Q.,Wuhan Aquaculture Science Research Institute | Wang G.,Wuhan Aquaculture Science Research Institute | And 3 more authors.
Genetics and Molecular Research | Year: 2015

Twenty-eight polymorphic microsatellite markers were developed from the transcriptome of Ancherythoculter nigrocauda. These loci were used to characterize the genotypes of 48 individuals. The observed number of alleles per locus ranged from 5 to 11, with an average of 7.7. Expected and observed heterozygosities ranged from 0.437 to 0.978 and from 0.373 to 1.000, respectively. Four of these polymorphic microsatellite loci (HWB14, HWB18, HWB24, and HWB30) deviated significantly from the Hardy-Weinberg equilibrium after use of the sequential Bonferroni correction (P < 0.05). Twenty of the 28 loci could be successfully amplified in Culter alburnus. These novel markers will be useful for germplasm resource conservation and management of A. nigrocauda and C. alburnus. © FUNPEC-RP. Source

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