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Deng G.,Huazhong Agricultural University | Deng G.,Yunnan University | Zhong X.,Huazhong Agricultural University | Zhang N.,Wuhan Agriculture and Science Institute | And 4 more authors.
PLoS ONE | Year: 2014

Sample preparation is key to the success of proteomics studies. In the present study, two sample preparation methods were tested for their suitability on the mature, recalcitrant leaves of six representative perennial plants (grape, plum, pear, peach, orange, and ramie). An improved sample preparation method was obtained: Tris and Triton X-100 were added together instead of CHAPS to the lysis buffer, and a 20% TCA-water solution and 100% precooled acetone were added after the protein extraction for the further purification of protein. This method effectively eliminates nonprotein impurities and obtains a clear two-dimensional gel electrophoresis array. The method facilitates the separation of high-molecular-weight proteins and increases the resolution of low-abundance proteins. This method provides a widely applicable and economically feasible technology for the proteomic study of the mature, recalcitrant leaves of perennial plants. © 2014 Gang et al. Source


An X.,Huazhong Agricultural University | Liao Y.,Huazhong Agricultural University | Zhang J.,Huazhong Agricultural University | Dai L.,Huazhong Agricultural University | And 5 more authors.
Plant Growth Regulation | Year: 2015

Transgenic technology is probably the best way to improve crops when traditional breeding is not feasible. Ramie is one such plant because the heterozygote complicates breeding efforts. This study is the first to report the successful production of transgenic ramie plants which is tolerant to drought and salt stress. In this study, the stress responsive gene SNAC1 (STRESS-RESPONSIVE NAC 1) was first introduced into ramie by Agrobacterium-mediated transformation which was using leaf midribs as explants. The overexpression of SNAC1 significantly enhanced drought and salt tolerance at the seedling and fiber maturation stages, via observation of phenotype and yield between transgenic plants and wild-type ones. SNAC1-overexpressing ramie plants also showed significantly improved tolerance to both drought and salt stresses at the rapid-growth stage. The transgenic plants had higher photosynthesis rates and relative water contents compared to the wild-type plants during the rapid-growth stage under drought and salt stress. Our study indicates that ramie’s tolerance to drought and salt stress can be improved through genetic engineering. © 2014, Springer Science+Business Media Dordrecht. Source


Liu L.-J.,Huazhong Agricultural University | Lao C.-Y.,Huazhong Agricultural University | Zhang N.,Huazhong Agricultural University | Zhang N.,Wuhan Agriculture and Science Institute | And 5 more authors.
Industrial Crops and Products | Year: 2013

We investigated a new ramie (Boehmeria nivea L.) harvest mode, which can resolve the bottleneck problems hindering the development of the ramie cultivation industry. In an experiment using cultivar 'Huazhu 4', we designed five ramie harvest modes with the conventional harvest mode as the control group. The range of ramie production was 2214.67-2533.67. kg/ha, which was lower than the 2724.33. kg/ha for conventional harvest (average reduction of 10%). The gel content of all treatments was higher than in the control. The range of average fiber diameter was 15.95 ± 0.28μm to 17.02 ± 0.39μm, fiber diameter in Mode C was optimal. For each harvest mode, the fiber breaking strength was greatest in the first ramie harvested. With the postponing of harvest time, fiber average breaking elongation rate were lower than control except for Modes B and D. In the first harvest (except for Mode D), fiber crystallinity for each mode was significantly different to the control, but not when harvested later. Generally, the average gel content of raw ramie, fiber breaking strength and fiber crystallinity was not significantly different to the control. Continuous harvesting mode was feasible for ramie production. © 2012. Source

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