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He Y.-H.,Huazhong Agricultural University | Ning G.-G.,Huazhong Agricultural University | Sun Y.-L.,Wuhan Academy of Agricultural Science | Hu Y.,Huazhong Agricultural University | And 2 more authors.
Molecular Breeding | Year: 2010

A male sterile line was isolated in marigold (Tagetes erecta L.) and cytological analysis determined this to be a novel genic male sterility trait (Tems). Through the use of amplified fragment length polymorphisms (AFLPs) and bulked segregant analysis (BSA), tightly linked markers of Tems were identified with a view towards a map-based cloning strategy. It was found that spontaneous homeotic conversion of floral organs was the underlying cause of the male sterility in this marigold line. Thus, petals of male sterile plants resembled sepal-like structures and the stamens were partially converted to styles, although without the full characteristics or function of the true style organs. We have constructed a fine marker-based map for the Tems gene. This is intended to provide a tool for marker assisted selection (MAS) strategies in hybrid breeding and map-based cloning strategies for the male sterility locus. We discuss the significance of this spontaneously derived genic male sterility trait relating to the homeotic conversion of floral organs in marigold. © 2009 Springer Science+Business Media B.V.

Li Z.,CAS Wuhan Botanical Garden | Zhang G.-S.,CAS Wuhan Botanical Garden | Liu Y.,CAS Wuhan Botanical Garden | Wan K.-Y.,CAS Wuhan Botanical Garden | And 2 more authors.
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban | Year: 2013

According to the topography of the cities in Hubei Province, soil samples were collected from the urban green space in two mountainous cities (Enshi and Shiyan), three hilly cities (Jing-men, Xiangfan and Yichang), and five plain cities (Wuhan, Xiaogan, Xianning, Jingzhou, Suizhou and Huangshi). Within each city, subsoil samples were taken in accordance with four different types of land use, including park, residential, institutional (school, hospital and government, etc.), and roadside. In the main cities in Hubei, the soil pH of urban green space was averagely 7.9, being obviously higher than that of natural soils, while the soil organic matter content was rather low (6.8 g x kg(-1)). The soil available N and P contents were at a low level, while the soil available trace element (Ca, Mg, S, Fe, Cu, Mn, Zn and B) contents were moderate. Land use type had significant effects on the soil nutrient contents in plain cities. The soil pH in the residential green space was significantly higher than that in the park, roadside and institutional green space, while the contents of soil available trace elements (S, Cu, Mn and Zn) in roadside green space were significantly higher than those of green space in the other land use types. Park green space had the lowest soil nutrient contents. There existed significant differences in the soil nutrient contents among the cities with different topography. The soil organic matter, NH4-N, available K and P, and Ca, Mg, S, Fe, Cu and Mn contents were significantly higher in plain cities than in mountainous cities.

Chen H.,Huazhong Agricultural University | Cheng L.,Wuhan Academy of Agricultural Science | Yang S.,Huazhong Agricultural University | Liu X.,Huazhong Agricultural University | And 5 more authors.
Molecular Immunology | Year: 2010

The S100A12 gene belongs to the S100 family of genes, which are specific to vertebrates. It is involved in many inflammatory diseases of human and has been considered as a powerful diagnostic gene. In the present study, we identified the porcine S100A12 (p. S100A12) gene, provided evidence that p. S100A12 is located on chromosome 4 and is closely linked to SW512. We show that p. S100A12 is expressed preferentially in immune organs/tissues, e.g., bone marrow, spleen, and inguinal lymph nodes. Expression of the p. S100A12 gene is dramatically induced in porcine whole blood cultures by both lipopolysaccharide (LPS) and polyinosine-polycytidylic acid (Poly(I:C)). Elevated expression of p. S100A12 is also correlated with in vivo infection with porcine circovirus type 2 (PCV-2) from at least 48. h post infection. By analyzing a series of p. S100A12 promoter reporter constructs, we have defined two crucial regions (-1013 to -590, -135 to -50) that are responsible for LPS- and Poly(I:C)-induced transcriptional activation, and demonstrated that the LPS/Poly(I:C)-PKC-C/EBPb-p. S100A12 pathway may play a critical role in the transcription of the p. S100A12. © 2010 Elsevier Ltd.

Huang H.-J.,Huazhong Agricultural University | Huang H.-J.,Wuhan Academy of Agricultural Science | Gao Q.-S.,Wuhan Academy of Agricultural Science | Qian Y.-G.,Wuhan Academy of Agricultural Science | And 6 more authors.
Cell Biology International | Year: 2010

ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly proteins. This study investigates the feasibility of implanting mES-dK (mouse ES-derived keratinocytes-like) cells stably transfected with a mammary gland special expression vector for the PBD-1 (porcine beta-defensin 1) in developing mammary glands. Our aim was to assess the ability of cell grafting to improve functional outcome following partial damage of the breast, also on the breast modification mammary tissue in mice for the production of PBD-1 protein secreted in the milk. Our results showed that the ratios of the surviving cells labelled with the myoepithelial or luminal cell markers, EMA (epithelial membrane antigen) and CALLA, were 41.7±15.2% and 28.4±9.6%, respectively, which revealed that transplanted mES-dK cells survived, integrated in vivo and differentiated into myoepithelial or luminal cells. In addition, Western blot analysis showed that 37.5% (3 out of 8) female transplanted mice had PBD-1 expression in their milk and reached 0.4998, 0.5229 and 0.5195 μg/ml, respectively. © The Author(s) Journal compilation © 2010 Portland Press Limited.

Huang H.-J.,Wuhan Academy of Agricultural Science | Gao Q.-S.,Wuhan Academy of Agricultural Science | Qian Y.-G.,Wuhan Academy of Agricultural Science | Zhang Y.-D.,Huazhong Agricultural University | And 3 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2011

Embryonic stem (ES) cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES cells to improve functional outcome following mammary gland injury. This study investigates the feasibility of implanting mouse ES cells labeled with enhanced green fluorescence protein in the developing mammary glands in order to acquire lineage-committed cells in mammary (mammary gland epithelial cell or luminal cell). Cells implanted in high numbers (5×10M6 cells per mammary gland) survived in the majority of the mice and nearly 38.4% of the surviving cells were CK18+ at 15th week following the transplantation. Our results may provide a technique instrument on advanced therapy of breast diseases and the mammary regeneration after breast ablated partly. © The Society for In Vitro Biology 2010.

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