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Chen H.,Huazhong Agricultural University | Lunney J.K.,U.S. Department of Agriculture | Cheng L.,Wuhan Academy of Agricultural Science | Li X.,Huazhong Agricultural University | And 3 more authors.
Developmental and Comparative Immunology | Year: 2011

S100 calcium-binding protein A8 (S100A8) and S100 calcium-binding protein A9 (S100A9) are pivotal mediators of inflammatory and protective anti-infection responses for the mammalian host. In this study, we present the molecular cloning of porcine S100A8 (p. S100A8) and porcine S100A9 (p. S100A9). Both genes comprise 3 exons and 2 introns and are located on pig chromosome 4q21-q23 (closely linked to SW512). Homology comparison to other mammalian species affirmed that critical functional amino acids for post-transcriptional modification, inflammatory regulation, and formation of heterodimeric complexes exist in pS100A8 and pS100A9. Under normal conditions, both genes are preferentially expressed in porcine immune or immune-related organs, e.g., bone marrow, spleen, lymph nodes, and lung. Upon stimulation in porcine whole blood cultures with LPS or Poly(I:C), they are dramatically induced. Interestingly, the maximum increase of mRNA levels in blood cultures of Meishan pigs is significantly greater than that in Duroc pigs. We previously showed that p. S100A8 and p. S100A9 mRNA were up-regulated following Haemophilus parasuis (HPS) infection. We herein further confirm their up-regulation at the protein level in multiple HPS infected tissues (spleen, lung and liver). Functional cluster and network analysis based on our previous microarray data discovered that CEBPB may be one of the key transcription factors. A pS100A8/pS100A9-CASP3-SLC1A2 pathway regulating lipid metabolism was found. Both of their pro- and anti-inflammatory functions in response to HPS infection are highlighted. © 2010 Elsevier Ltd.


Lu S.,Huazhong Agricultural University | Lu S.,Wuhan Academy of Agricultural Science | Xiang M.,Wuhan Academy of Agricultural Science | Dan H.,Huazhong Agricultural University | And 5 more authors.
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy | Year: 2013

The glycoprotein E (gE) of pseudorabies virus (PRV) is known to be an important marker protein in the control and eradication of Aujeszky's disease. In this study, BALB/c mice were immunized with gE-deleted PRV as tolerogen and with wild-type PRV as immunogen. The spleen cells from the immunized mice were then fused with the myeloma cell line Sp2/0. Two hybridoma cell lines that could stably secrete the monoclonal antibody (MAb) against gE were achieved by using indirect ELISA screening and subcloning three times; they were named 1D2 and 2B2. Indirect immunofluorescence assay (IFA) revealed that the MAbs were specifically against gE of PRV. MAbs 1D2 and 2B2 were subgroup IgG1. The MAbs obtained in this study provide useful tools for the development of differential diagnostic methods for PRV. © Mary Ann Liebert, Inc.


He Y.-H.,Huazhong Agricultural University | Ning G.-G.,Huazhong Agricultural University | Sun Y.-L.,Wuhan Academy of Agricultural science | Hu Y.,Huazhong Agricultural University | And 2 more authors.
Molecular Breeding | Year: 2010

A male sterile line was isolated in marigold (Tagetes erecta L.) and cytological analysis determined this to be a novel genic male sterility trait (Tems). Through the use of amplified fragment length polymorphisms (AFLPs) and bulked segregant analysis (BSA), tightly linked markers of Tems were identified with a view towards a map-based cloning strategy. It was found that spontaneous homeotic conversion of floral organs was the underlying cause of the male sterility in this marigold line. Thus, petals of male sterile plants resembled sepal-like structures and the stamens were partially converted to styles, although without the full characteristics or function of the true style organs. We have constructed a fine marker-based map for the Tems gene. This is intended to provide a tool for marker assisted selection (MAS) strategies in hybrid breeding and map-based cloning strategies for the male sterility locus. We discuss the significance of this spontaneously derived genic male sterility trait relating to the homeotic conversion of floral organs in marigold. © 2009 Springer Science+Business Media B.V.


Tang M.,Wuhan Academy of Agricultural Science | Zhu J.-H.,Wuhan Academy of Agricultural Science | Ren J.,Wuhan Academy of Agricultural Science | Shi X.-F.,Wuhan Academy of Agricultural Science | Peng J.-G.,Wuhan Academy of Agricultural Science
Proceedings - 2014 IEEE Workshop on Advanced Research and Technology in Industry Applications, WARTIA 2014 | Year: 2014

China is the largest producer and consumer of vegetables in the world. Manual indenting and sowing of small vegetable seeds in plug trays is a slow and labor-intensive operation. Therefore, seeding operation of plug trays should be mechanized to increase the capacity of the rapidly expanding nursery industry. A tray precision seeding machine is one of the key devices used in industrialized plug seedling production. To reduce the miss-seeding and reseeding rates of seeders, we tested main varieties of different vegetables, such as cucumber, eggplant, pepper, and tomato, by obtaining their seeds (grain diameter ≤7mm) as materials. The physical characteristics (geometrical size, 1000-kernel mass, rest angle, and sliding friction angle) and aerodynamic characteristic (floating speed) of these vegetables were measured. The results showed that the average geometrical sizes (length, width, and thickness) were 9.00, 3.87, and 1.42 mm (cucumber); 3.07, 2.63, and 0.92 mm (eggplant); 3.96, 3.40, and 0.76 mm (pepper); and 3.23, 2.36, and 0.63 mm (tomato). One-thousand kernel mass values were 26.49, 4.27, 5.94, and 2.80 g, respectively. Rest angles were 19.36°, 19.83°, 20.56°, and 26.24°, respectively. Sliding friction angles (stainless steel plate) were 21.80°, 24.95°, 25.66°, and 31.82°, respectively. Finally, floating speed rates were 6.38, 5.02, 4.24, and 3.78m/s, respectively. © 2014 IEEE.


Huang H.-J.,Wuhan Academy of Agricultural Science | Gao Q.-S.,Wuhan Academy of Agricultural Science | Qian Y.-G.,Wuhan Academy of Agricultural Science | Zhang Y.-D.,Huazhong Agricultural University | And 3 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2011

Embryonic stem (ES) cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES cells to improve functional outcome following mammary gland injury. This study investigates the feasibility of implanting mouse ES cells labeled with enhanced green fluorescence protein in the developing mammary glands in order to acquire lineage-committed cells in mammary (mammary gland epithelial cell or luminal cell). Cells implanted in high numbers (5×10M6 cells per mammary gland) survived in the majority of the mice and nearly 38.4% of the surviving cells were CK18+ at 15th week following the transplantation. Our results may provide a technique instrument on advanced therapy of breast diseases and the mammary regeneration after breast ablated partly. © The Society for In Vitro Biology 2010.


Chen H.,Huazhong Agricultural University | Cheng L.,Wuhan Academy of Agricultural Science | Yang S.,Huazhong Agricultural University | Liu X.,Huazhong Agricultural University | And 5 more authors.
Molecular Immunology | Year: 2010

The S100A12 gene belongs to the S100 family of genes, which are specific to vertebrates. It is involved in many inflammatory diseases of human and has been considered as a powerful diagnostic gene. In the present study, we identified the porcine S100A12 (p. S100A12) gene, provided evidence that p. S100A12 is located on chromosome 4 and is closely linked to SW512. We show that p. S100A12 is expressed preferentially in immune organs/tissues, e.g., bone marrow, spleen, and inguinal lymph nodes. Expression of the p. S100A12 gene is dramatically induced in porcine whole blood cultures by both lipopolysaccharide (LPS) and polyinosine-polycytidylic acid (Poly(I:C)). Elevated expression of p. S100A12 is also correlated with in vivo infection with porcine circovirus type 2 (PCV-2) from at least 48. h post infection. By analyzing a series of p. S100A12 promoter reporter constructs, we have defined two crucial regions (-1013 to -590, -135 to -50) that are responsible for LPS- and Poly(I:C)-induced transcriptional activation, and demonstrated that the LPS/Poly(I:C)-PKC-C/EBPb-p. S100A12 pathway may play a critical role in the transcription of the p. S100A12. © 2010 Elsevier Ltd.


Tang M.,Wuhan Academy of Agricultural Science | Zhu J.-H.,Wuhan Academy of Agricultural Science | Ren J.,Wuhan Academy of Agricultural Science | Shi X.-F.,Wuhan Academy of Agricultural Science | Peng J.-G.,Wuhan Academy of Agricultural Science
Journal of Food, Agriculture and Environment | Year: 2015

China is the largest producer and consumer of vegetables in the world. Manual indenting and sowing of small vegetable seeds in plug trays is a slow and labor-intensive operation. Therefore, seeding operation of plug trays should be mechanized to increase the capacity of the rapidly expanding nursery industry. A tray precision seeding machine is one of the key devices used in industrialized plug seedling production. To reduce the miss-seeding and reseeding rates of seeders, we tested main varieties of different vegetables, such as cucumber, eggplant, pepper, and tomato, by obtaining their seeds (grain diameter <7 mm) as materials. The physical characteristics (geometrical size, 1000-kernel mass, rest angle, and sliding friction angle) and aerodynamic characteristic (floating speed) of these vegetable seeds were measured. The results showed that the average geometrical sizes (length, width, and thickness) were 9.00, 3.87, and 1.42 mm (cucumber);3.07, 2.63, and 0.92 mm (eggplant);3.96, 3.40, and 0.76 mm (pepper);and 3.23, 2.36, and 0.63 mm (tomato).One-thousand kernel mass values were 26.49, 4.27, 5.94, and 2.80 g, respectively. Rest angles were 19.36°, 19.83°, 20.56°, and 26.24°, respectively. Sliding friction angles (stainless steel plate) were 21.80°, 24.95°, 25.66°, and 31.82°, respectively. Finally, floating speed rates were 6.38, 5.02, 4.24, and 3.78 ms-1, respectively. © 2015, World Food Ltd. and WFL Publishers. All rights reserved.


PubMed | Huazhong Agricultural University and Wuhan Academy of Agricultural Science
Type: Journal Article | Journal: Gene | Year: 2014

Avian -defensins (AvBDs) are a family of small antimicrobial peptides that play important roles in the innate immunity of birds. Herein, we report on two new ostrich AvBD genes, AvBD2 and AvBD7, which were isolated from the bone marrow of ostriches (Struthio camelus). The coding regions of ostrich AvBD2 and AvBD7 comprised 195 bp and 201bp, which encoded 64 and 66 amino acids, respectively. Homology analysis showed that ostrich AvBD2 had the highest similarity (up to 86%) with the swan goose (Anser cygnoides) AvBD2, while ostrich AvBD7 shared the highest similarity (up to 81%) with chicken AvBD7. Analysis of the codon-usage bias showed that the two ostrich AvBDs had different codon-usage patterns from other AvBDs. The two synthetic AvBD peptides exhibited antibacterial activities against both Gram-positive and Gram-negative bacteria, and these activities decreased significantly in the presence of 100mM NaCl (P<0.01). Real-time reverse transcription-polymerase chain reaction analysis showed that AvBD2 and AvBD7 were widely expressed at different levels in 17 different tissues. This is the first report of the nucleotide sequences of ostrich AvBDs. Further investigations of these two AvBDs may help us to gain new insights into the immune defense system of the ostrich and to make subsequent therapeutic use of ostrich defensins.


PubMed | CAS Wuhan Botanical Garden and Wuhan Academy of Agricultural Science
Type: Journal Article | Journal: Ying yong sheng tai xue bao = The journal of applied ecology | Year: 2014

According to the topography of the cities in Hubei Province, soil samples were collected from the urban green space in two mountainous cities (Enshi and Shiyan), three hilly cities (Jing-men, Xiangfan and Yichang), and five plain cities (Wuhan, Xiaogan, Xianning, Jingzhou, Suizhou and Huangshi). Within each city, subsoil samples were taken in accordance with four different types of land use, including park, residential, institutional (school, hospital and government, etc.), and roadside. In the main cities in Hubei, the soil pH of urban green space was averagely 7.9, being obviously higher than that of natural soils, while the soil organic matter content was rather low (6.8 g x kg(-1)). The soil available N and P contents were at a low level, while the soil available trace element (Ca, Mg, S, Fe, Cu, Mn, Zn and B) contents were moderate. Land use type had significant effects on the soil nutrient contents in plain cities. The soil pH in the residential green space was significantly higher than that in the park, roadside and institutional green space, while the contents of soil available trace elements (S, Cu, Mn and Zn) in roadside green space were significantly higher than those of green space in the other land use types. Park green space had the lowest soil nutrient contents. There existed significant differences in the soil nutrient contents among the cities with different topography. The soil organic matter, NH4-N, available K and P, and Ca, Mg, S, Fe, Cu and Mn contents were significantly higher in plain cities than in mountainous cities.


PubMed | Wuhan Academy of Agricultural Science
Type: Journal Article | Journal: In vitro cellular & developmental biology. Animal | Year: 2011

Embryonic stem (ES) cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES cells to improve functional outcome following mammary gland injury. This study investigates the feasibility of implanting mouse ES cells labeled with enhanced green fluorescence protein in the developing mammary glands in order to acquire lineage-committed cells in mammary (mammary gland epithelial cell or luminal cell). Cells implanted in high numbers (5 10(6) cells per mammary gland) survived in the majority of the mice and nearly 38.4% of the surviving cells were CK18(+) at 15th week following the transplantation. Our results may provide a technique instrument on advanced therapy of breast diseases and the mammary regeneration after breast ablated partly.

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