Wuchang Hospital of Wuhan City

Wuhan, China

Wuchang Hospital of Wuhan City

Wuhan, China
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Wang X.,Wuchang Hospital of Wuhan City | Wang M.-L.,Wuchang Hospital of Wuhan City | Lu X.-Y.,Wuchang Hospital of Wuhan City | Zhang P.,Wuchang Hospital of Wuhan City | And 2 more authors.
World Chinese Journal of Digestology | Year: 2015

AIM: To investigate the potential effects of glucagon-like peptide-1 (GLP-1) on palmitic acid (PA) induced apoptosis of hepatic L02 cells and the underlying mechanism.METHODS: L02 cells were stimulated with different levels (0.125, 0.250 and 0.500 mmol/L) of PA for different durations (12, 24 and 48 h) in the presence or absence of GLP-1. Cell Counting Kit-8 (CCK-8) assay was used to analyze the inhibitory effects on growth of L02 cells, and terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) assay was used to observe the apoptotic rate of the cells. The expression of c-Jun N-terminal kinase (JNK), c-Jun, p-JNK and p-c-Jun proteins was detected by Western blot assay.RESULTS: The growth of the L02 cells was significantly inhibited by PA in vitro, and PA induced the expression of p-JNK and p-c-Jun. GLP-1 suppressed the activation of JNK and c-Jun induced by PA.CONCLUSION: PA can inhibit the proliferationof L02 cells and induce cell apoptosis. The JNK signaling pathway is probably involved in the mechanism of PA induced apoptosis. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Wang X.,Wuhan University | Yu H.,Wuhan University | Lu X.,Wuchang Hospital of Wuhan City | Zhang P.,Wuchang Hospital of Wuhan City | And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2014

Gastric cancer (GC) is the second common cause of cancer-related death worldwide. microRNAs (miRNAs) play important roles in the carcinogenesis of GC. Here, we found that miR-22 was significantly decreased in GC tissue samples and cell lines. Ectopic overexpression of miR-22 remarkably suppressed cell proliferation and colony formation of GC cells. Moreover, overexpression of miR-22 significantly suppressed migration and invasion of GC cells. CD151 was found to be a target of miR-22. Furthermore, overexpression of CD151 significantly attenuated the tumor suppressive effect of miR-22. Taken together, miR-22 might suppress GC cells growth and motility partially by inhibiting CD151. © 2014 Elsevier Inc. All rights reserved.


Wang X.,Wuhan University | Wang M.L.,Wuchang Hospital of Wuhan City | Zhou L.Y.,Wuchang Hospital of Wuhan City | Lu X.Y.,Wuchang Hospital of Wuhan City | And 2 more authors.
Clinical and Translational Oncology | Year: 2013

Purpose: This randomized phase II study was conducted to compare the efficacy and safety of paclitaxel with S-1 (PS) vs. S-1 in patients with advanced gastric cancer (AGC). Methods: Eighty-two (82) patients were 1:1 randomly assigned to oral S-1 (daily for 2 weeks, every 4 weeks' cycle) or S-1 (daily for 2 weeks, every 4 weeks' cycle) plus paclitaxel (on day 1, 8 and 15 of a 4 weeks' cycle). S-1 was orally administered with a fixed quantity according to body surface area (BSA), while paclitaxel was given 60 mg/m2 i.v. daily through an implanted catheter. The primary endpoint was overall survival (OS). Secondary endpoints included progression-free survival (PFS), overall responsible rates and safety. Results: The median OS with PS versus S-1 monotherapy was 14.0 versus 11.0 months (P = 0.02), survival at 12 months was 61.0 % in the PS group and 46.3 % in the S-1 group. Median PFS was also significantly longer in the PS group (6.0 months) than in the S-1 group (4.0 months). The overall response rate was determined in 82 evaluable patients, and was significantly higher (P = 0.04) with PS (19 patients, 46.3 %) than with S-1 monotherapy (10 patients, 24.4 %). PS was well tolerated with no treatment-related deaths, all were grade 3-4 gastrointestinal toxicities, including anorexia, nausea, and diarrhea developed in less than 10 % of the patients. Conclusions: Combination chemotherapy of paclitaxel with S-1 is well tolerated and active in AGC patients. Further investigation with comparative trials is needed for confirmation. © 2013 Federación de Sociedades Españolas de Oncología (FESEO).


Wang X.,Wuchang Hospital of Wuhan City | Wang M.-L.,Wuchang Hospital of Wuhan City | Lu X.-Y.,Wuchang Hospital of Wuhan City | Zhang P.,Wuchang Hospital of Wuhan City | And 2 more authors.
World Chinese Journal of Digestology | Year: 2014

AIM: To investigate the effect of beraprost sodium (BS) on the c-Jun N-terminal kinase (c-JNK) signal pathway in rats with severe acute pancreatitis (SAP). METHODS: Forty-eight SD rats were randomly divided into a sham operation group (normal control), an SAP group and a BS group. SAP was induced by retrograde injection of 5% sodium tauroeholate. BS was given at a dose of 20 μg/kg at the time of pancreatitis induction in the BS group. At 1, 3, 6, and 12 h after SAP was successfully induced, the animals were killed. Levels of tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) in pancreatic tissue were detected by ELISA, c-JNK mRNA expression was detected by RT-PCR, and c-JNK and phosporylated c-JNK (p-c-JNK) protein in pancreatic tissue was examined by Western blot. RESULTS: Compared with the normal control group, the levels of TNF-α, IL-6, c-JNK mRNA, c-JNK protein and p-c-JNK protein in pancreatic tissues were significantly enhanced in the SAP group (TNF-α: 458.7 pg/mL ± 36.9 pg/mL vs 197.1 pg/mL ± 5.3 pg/mL; IL-6: 525.6 pg/mL ± 12.6 pg/mL vs 211.4 pg/mL ± 3.5 pg/mL; c-JNK mRNA: 2.60 ± 0.09 vs 0.91 ± 0.59; c-JNK protein: 1.52 ± 0.41 vs 0.98 ± 0.11; p-c-JNK protein: 1.53 ± 0.45 vs 0.98 ± 0.08, P < 0.05 for all). Compared with the SAP group, the levels of TNF-α, IL-6, c-JNK mRNA, c-JNK protein and p-c-JNK protein were significantly decreased in the BS group (TNF-α: 211.7 pg/ mL ± 3.4 pg/mL vs 458.7 pg/m L ± 36.9 pg/ mL; IL-6: 468.7 pg/mL ± 34.9 pg/mL vs 525.6 pg/mL± 12.6 pg/mL; c-JNK mRNA: 1.93 ± 0.14 vs 2.60 ± 0.09; c-JNK protein: 1.32 ± 0.35 vs 1.52 ± 0.41; p-c-JNK protein: 1.22 ± 0.37 vs 1.53 ± 0.45, P < 0.05 for all). CONCLUSION: BS can alleviate the inflammatory response in SAP rats by down-regulating c-JNK signaling. © 2014 Baishideng Publishing Group Inc. All rights reserved.


PubMed | Wuchang Hospital of Wuhan City, Wuhan University and Huazhong University of Science and Technology
Type: Journal Article | Journal: Biochemical and biophysical research communications | Year: 2014

Gastric cancer (GC) is the second common cause of cancer-related death worldwide. microRNAs (miRNAs) play important roles in the carcinogenesis of GC. Here, we found that miR-22 was significantly decreased in GC tissue samples and cell lines. Ectopic overexpression of miR-22 remarkably suppressed cell proliferation and colony formation of GC cells. Moreover, overexpression of miR-22 significantly suppressed migration and invasion of GC cells. CD151 was found to be a target of miR-22. Furthermore, overexpression of CD151 significantly attenuated the tumor suppressive effect of miR-22. Taken together, miR-22 might suppress GC cells growth and motility partially by inhibiting CD151.


Wang X.,Wuchang Hospital of Wuhan City | Wang M.-L.,Wuchang Hospital of Wuhan City | Lu X.-Y.,Wuchang Hospital of Wuhan City | Zhang P.,Wuchang Hospital of Wuhan City | And 2 more authors.
World Chinese Journal of Digestology | Year: 2014

Aim: To elucidate the potential effects of c-Jun amino terminal kinase (JNK) inhibitor SP600125 on apoptosis induced by free fatty acids (FFAs) in hepatic stellate cells and the possible mechanism.Methods: MTT assay was used to analyze the inhibitory effect of FFAs on growth of hepatic stellate cells, and flow cytometry was used to observe the apoptotic rate of the cells. The expression of p-JNK and p-c-Jun proteins was detected by Western blot assay.Results: The growth of hepatic stellate cells was significantly inhibited by FFAs in vitro, and FFAs increased the expression of p-JNK and p-cJun. JNK inhibitor SP600125 suppressed FFAinduced up-regulation of p-JNK and p-c-Jun.Conclusion: FFAs can inhibit the proliferation of hepatic stellate cells and induce cell apoptosis, which involves the JNK signaling pathway. © 2014 Baishideng Publishing Group Inc. All rights reserved.


Wang X.,Wuchang Hospital of Wuhan City | Lu X.-Y.,Wuchang Hospital of Wuhan City | Zhang P.,Wuchang Hospital of Wuhan City | Wang M.-L.,Wuchang Hospital of Wuhan City | And 2 more authors.
World Chinese Journal of Digestology | Year: 2014

AIM: To investigate the potentially protective effect of SP600125, a selective c-Jun N-terminal kinase inhibitor, in a rat model of nonalcoholic steatohepatitis (NASH) induced with a high fat diet.METHODS: Forty-eight Sprague-Dawley rats were randomly divided into three groups: a normal control group (NC group), a highfat model group (HF group) and an SP600125 treatment group (SP group). All rats were subjected to a percutaneous superior mesenteric vein retention catheter operation and fed a standard diet for 4 wk. The HF group was then fed an HF diet and treated with dimethyl sulfoxide, while the SP group was fed an HF diet and treated with SP600125 (50 mg/kg) once per day.RESULTS: Feeding an HF diet successfully induced NASH in rats, with varying degrees of hepatic steatosis and hepatic inflammation. SP600125 treatment substantially decreased the level of inflammation and the expression of c-Jun and p-cJun proteins.CONCLUSION: SP600125 has the potential to remarkably attenuate steatosis and inflammation and may be a novel therapeutic drug against NASH. © 2014 Baishideng Publishing Group Inc. All rights reserved.


Cheng X.H.,Guanggu Guanshan Campus of the Third Hospital of Wuhan | Chen W.X.,Guanggu Guanshan Campus of the Third Hospital of Wuhan | Zeng W.,Wuchang Hospital of Wuhan City
World Chinese Journal of Digestology | Year: 2015

AIM: To observe the gastrointestinal adverse reactions of Hemabate injection at different sites in cesarean section and discuss nursing measures. METHODS: A retrospective analysis was performed of 120 women who received Hemabate injection at different sites (group A: intrauterine injection; group B: intramuscular injection intothe deltoid muscle) in cesarean section between January 2011 and December 2012. Gastrointestinal adverse reactions and nursing measures were analyzed. RESULTS: The incidences of bleeding at 2 and 24 h postpartum showed no significant difference between the two groups (P > 0.05). The incidence of gastrointestinal adverse reactions was significantly lower in group A than in group B (15.7% vs 42.0%, P < 0.05). CONCLUSION: Intrauterine injection of Hemabate is superior to intramuscular injection into the deltoid muscle with regard to lower incidence of gastrointestinal adverse reactions, although the incidences of postpartum bleeding are similar between the two groups. © 2015 Baishideng Publishing Group Inc. All rights reserved.

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