Wound Management Innovation Cooperative Research Center

Brisbane, Australia

Wound Management Innovation Cooperative Research Center

Brisbane, Australia
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Szili E.J.,University of South Australia | Hong S.-H.,University of South Australia | Oh J.-S.,Meijo University | Gaur N.,University of South Australia | And 3 more authors.
Trends in Biotechnology | Year: 2017

Electrically generated cold atmospheric plasma is being intensively researched for novel applications in biology and medicine. Significant attention is being given to reactive oxygen and nitrogen species (RONS), initially generated upon plasma-air interactions, and subsequently delivered to biological systems. Effects of plasma exposure are observed to millimeter depths within tissue. However, the exact nature of the initial plasma-tissue interactions remains unknown, including RONS speciation and delivery depth, or how plasma-derived RONS intervene in biological processes. Herein, we focus on current research using tissue and cell models to learn more about the plasma delivery of RONS into biological environments. We argue that this research is vital in underpinning the knowledge required to realize the full potential of plasma in biology and medicine. Physical effects of plasma can be seen to depths of several hundred micrometers within tissue.Plasma-derived RONS are likely to be delivered millimeters into tissues.Speciation reveals that RONS delivered by plasma into tissue fluid and tissue are predominately stable secondary RONS - for example, H2O2, NO2 -, and NO3 -.The plasma generation of RONS within a hydrated target is influenced by the target matrix that can enhance or reduce the RONS concentrations and act as a reservoir of RONS.It is likely that the concentration of these plasma-derived RONS exceeds hundreds of micromoles, even at depths of several millimeters within tissue.Oxygen concentration at the time of plasma treatment significantly influences RONS generation within a hydrated proteinaceous target. © 2017 Elsevier Ltd.


Wager L.J.,Wound Management Innovation Cooperative Research Center | Wager L.J.,Queensland University of Technology | Murray R.Z.,Queensland University of Technology | Thompson E.W.,Queensland University of Technology | And 4 more authors.
Cell Adhesion and Migration | Year: 2017

The scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue of choice. Here we present an adaptation that extends the utility of this model to encompass the collection of cells from the leading edge of migrating epithelial sheets making available explorative biochemical analyses. The method is scalable and does not require expensive apparatus, making it suitable for large and small laboratories alike. We detail the application of our method and exemplify proof of principle data derived from primary human keratinocyte cultures. © 2017 The Author(s). Published with license by Taylor & Francis


Biva I.J.,University of South Australia | Biva I.J.,Wound Management Innovation Cooperative Research Center | Ndi C.P.,University of South Australia | Griesser H.J.,University of South Australia | And 2 more authors.
Journal of Ethnopharmacology | Year: 2016

Ethnopharmacological relevance For traditional medicinal purposes Aboriginal Australians have utilised numerous plant species, Eremophila alternifolia is among the most prominent. Traditionally, fresh leaves, leaf-infusions and handmade leaf-pastes have been used as both external and internal preparations to provide relief from a variety of conditions. Preparations of the species have been used to treat various infections of skin, eyes and throat including the treatment of septic wounds. These usages suggest that the plant contains antibacterial compounds; however, to date they have not been isolated and identified. Aim of the study The present study aimed to identify antibacterial compounds from this important traditionally recorded medicinal species. Materials and methods Bioassay-guided fractionation was used to isolate compounds from the crude leaf-extract. Antibacterial activity of pure compounds was assessed through broth microdilution method by determining both minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). Structure elucidation was performed using spectroscopic techniques such as 1D and 2D nuclear magnetic resonance spectroscopy and high resolution mass spectrometry. Results Four compounds have been isolated from the leaf-extract; they include previously known flavanones [pinobanksin (1), pinobanksin-3-acetate (2) and pinobanksin-3-cinnamate (3)] and a serrulatane diterpene, 8-hydroxyserrulat-14-en-19-oic acid (4). While compound 4 had been found in other Eremophilas, flavanones 2 and 3 are identified for the first time from the genus Eremophila. The flavanone 3 is the most promising antibacterial compound with significant activity (10-20 μM) against strains of the Gram-positive bacterium Staphylococcus aureus including methicillin resistant and biofilm forming strains. No activity was observed for any isolated compounds against the Gram-negative bacterium Escherichia coli. Conclusion The antibacterial activity of the crude extract of E. alternifolia and of the isolated compounds against Gram-positive bacteria provides a Western scientific explanation of the therapeutic modality of this plant species in traditional Aboriginal medicinal practice. © 2016 Elsevier Ireland Ltd. All rights reserved.


Ghaedizadeh A.,RMIT University | Ghaedizadeh A.,Wound Management Innovation Cooperative Research Center | Shen J.,RMIT University | Shen J.,Wound Management Innovation Cooperative Research Center | And 5 more authors.
Materials and Design | Year: 2017

The phenomenon of negative linear compressibility has attracted much interest because of its unusual deformation features with many potential applications. However, the design and fabrication of materials and structures with negative linear compressibility are limited. In this paper, we proposed two approaches to designing and fabricating new composite structures with negative linear compressibility. The effectiveness of the proposed design approaches was validated experimentally by applying uniformly distributed pressure to all surfaces of bulk specimens. The deformation features, strain history, and the effective area reduction of the specimens were analyzed from the experimental data. The results clearly demonstrated the feasibility of the proposed designing and manufacturing approaches for realizing composites with negative linear compressibility. © 2017 Elsevier Ltd


van Netten J.J.,Queensland University of Technology | van Netten J.J.,Wound Management Innovation Cooperative Research Center | Baba M.,Independent Consultant | Lazzarini P.A.,Queensland University of Technology | And 2 more authors.
Systematic Reviews | Year: 2017

Background: Diabetic foot disease is associated with major morbidity, mortality, costs, and reduction of a person's quality of life. Investigating the epidemiology of diabetic foot disease is the backbone of diabetic foot research and clinical practice, yet the full burden of diabetic foot disease in Australia is unknown. This study aims to describe the protocol for a systematic review of the epidemiology of diabetic foot disease and diabetes-related lower-extremity amputation in Australia. Methods-search: The systematic review will be performed according to the Preferred Reporting Items for Systematic Review and Meta-Analyses guidelines. PubMed and EMBASE will be searched for publications in any language and without restrictions to date. Two independent investigators will screen publications for eligibility, with publications reporting Australian population-based incidence or prevalence of diabetic foot disease or diabetes-related lower-extremity amputation to be included. Additionally, a forward literature search will be performed in Google Scholar, and a grey literature search will be performed to identify government publications. Methods-assessment: Quality assessment will be performed using customised checklists. The summary statistic used for each study will be an incidence or prevalence proportion of diabetic foot disease or diabetes-related lower-extremity amputation. The standard error for each proportion will be calculated. A meta-analysis will be performed when three or more publications of adequate quality, reporting on similar outcomes and in similar populations, are identified. Discussion: The results of this systematic review can be used to adequately inform stakeholders in the field of diabetic foot disease on the extent of the problem in incidence and prevalence of diabetic foot disease in Australia, and to help guide appropriate use of resources to reduce the burden of this disease. Systematic review registration: PROSPERO CRD42016050740. © 2017 The Author(s).


Burgess J.D.,University of Queensland | Burgess J.D.,Wound Management Innovation Cooperative Research Center | Cameron C.M.,Griffith University | Watt K.,James Cook University | Kimble R.M.,University of Queensland
Trials | Year: 2016

Background: Globally, burns are the fifth leading cause of non-fatal children's injuries, and the leading cause of childhood burns is hot beverage scalds. Although there have been a number of programmes aimed at preventing scalds in children, very few have specifically addressed hot beverage scalds, and fewer have reported a reduction in injury rates. In Australia, hot beverage scalds account for 18 % of all childhood burns - a figure that has remained constant for the past decade. Innovative new technologies, such as Smartphone applications (apps), present a novel way for delivering individual-level injury prevention messages. The low cost, scalability and broad reach make this technology an ideal channel for health interventions. One of the latest methods being used in health-related apps aimed at behaviour change is gamification. Gamification uses the gaming principles of rewards, competition and personalisation to engage participants and motivate them towards preferred behaviours. This intervention will use a Smartphone app-based platform that combines gamification and behaviour-change strategies to increase knowledge and awareness of hot beverage scald risks and burn first aid among mothers of young children. Methods/design: This is a two-group, parallel, single-blinded randomised control trial (RCT) to evaluate the efficacy of a Smartphone app-based injury prevention intervention. The primary outcome measure is change in knowledge. Change in knowledge is measured in three components: knowledge of correct burns first aid; knowledge of the main cause of burns/scalds in children aged 0-15yrs; knowledge of the main age group at risk for burns/scalds. The secondary outcome measures relate to the gamification methods, measuring participants frequency of engagement with the Cool Runnings app. Queensland-based mothers aged 18+ years who own a Smartphone and have at least one child aged 5-12 months are eligible to participate. Discussion: To our knowledge, this is the first study to evaluate an app-based delivery of injury prevention messages, and the first study to test the efficacy of gamification techniques in an injury prevention intervention. If this intervention is found to be effective, this RCT will provide a platform for targeting other childhood injury prevention campaigns. Trial registration: This trial was registered on 14 January 2016 with the Australian New Zealand Clinical Trials Registry ( ACTRN12616000019404 ). © 2016 Burgess et al.


PubMed | Wounds West Wound Healing Institute Australia, Queensland University of Technology, Bundall Medical Center, Silver Chain Group and 2 more.
Type: Journal Article | Journal: International wound journal | Year: 2016

Chronic wounds cost the Australian health system at least US$285 billion per year. Wound care services in Australia involve a complex mix of treatment options, health care sectors and funding mechanisms. It is clear that implementation of evidence-based wound care coincides with large health improvements and cost savings, yet the majority of Australians with chronic wounds do not receive evidence-based treatment. High initial treatment costs, inadequate reimbursement, poor financial incentives to invest in optimal care and limitations in clinical skills are major barriers to the adoption of evidence-based wound care. Enhanced education and appropriate financial incentives in primary care will improve uptake of evidence-based practice. Secondary-level wound specialty clinics to fill referral gaps in the community, boosted by appropriate credentialing, will improve access to specialist care. In order to secure funding for better services in a competitive environment, evidence of cost-effectiveness is required. Future effort to generate evidence on the cost-effectiveness of wound management interventions should provide evidence that decision makers find easy to interpret. If this happens, and it will require a large effort of health services research, it could be used to inform future policy and decision-making activities, reduce health care costs and improve patient outcomes.


PubMed | University of South Australia and Wound Management Innovation Cooperative Research Center
Type: Journal Article | Journal: Journal of natural products | Year: 2015

The purpose of this study was to assess the biofilm-removing efficacy and inflammatory activity of a serrulatane diterpenoid, 8-hydroxyserrulat-14-en-19-oic acid (1), isolated from the Australian medicinal plant Eremophila neglecta. Biofilm breakup activity of compound 1 on established Staphylococcus epidermidis and Staphylococcus aureus biofilms was compared to the antiseptic chlorhexidine and antibiotic levofloxacin. In a time-course study, 1 was deposited onto polypropylene mesh to mimic a wound dressing and tested for biofilm removal. The ex-vivo cytotoxicity and effect on lipopolysaccharide-induced pro-inflammatory cytokine release were studied in mouse primary bone-marrow-derived macrophage (BMDM) cells. Compound 1 was effective in dispersing 12 h pre-established biofilms with a 7 log10 reduction of viable bacterial cell counts, but was less active against 24 h biofilms (approximately 2 log10 reduction). Compound-loaded mesh showed dosage-dependent biofilm-removing capability. In addition, compound 1 displayed a significant inhibitory effect on tumor necrosis factor alpha (TNF-) and interleukin-6 (IL-6) secretion from BMDM cells, but interleukin-1 beta (IL-1) secretion was not significant. The compound was not cytotoxic to BMDM cells at concentrations effective in removing biofilm and lowering cytokine release. These findings highlight the potential of this serrulatane diterpenoid to be further developed for applications in wound management.


PubMed | University of Sheffield, University of South Australia and Wound Management Innovation Cooperative Research Center
Type: Journal Article | Journal: ACS applied materials & interfaces | Year: 2016

Skin has a remarkable capacity for regeneration; however, with an ever aging population, there is a growing burden to the healthcare system from chronic wounds. Novel therapies are required to address the problems associated with nonhealing chronic wounds. Novel wound dressings that can encourage increased reepithelialization could help to reduce the burden of chronic wounds. A suite of chemically defined surfaces have been produced using plasma polymerization, and the ability of these surfaces to support the growth of primary human skin cells has been assessed. Additionally, the ability of these surfaces to modulate cell migration and morphology has also been investigated. Keratinocytes and endothelial cells were extremely sensitive to surface chemistry showing increased viability and migration with an increased number of carboxylic acid functional groups. Fibroblasts proved to be more tolerant to changes in surface chemistry; however, these cells migrated fastest over amine-functionalized surfaces. The novel combination of comprehensive chemical characterization coupled with the focus on cell migration provides a unique insight into how a materials physicochemical properties affect cell migration.


PubMed | Healthcare Improvement Unit, Metro North Hospital & Health Service, Metro North Hospital and Health Service, Queensland University of Technology and Wound Management Innovation Cooperative Research Center
Type: | Journal: International wound journal | Year: 2016

In addition to affecting quality of life, diabetic foot ulcers (DFUs) impose an economic burden on both patients and the health system. This study developed a Markov model to analyse the cost-effectiveness of implementing optimal care in comparison with the continuation of usual care for diabetic patients at high risk of DFUs in the Australian setting. The model results demonstrated overall 5-year cost savings (AUD 910011 for those aged 35-54, $939160 for those aged 55-74 and $1239497 for those aged 75 or older) and improved health benefits measured in quality-adjusted life years (QALYs) (013 QALYs, 013 QALYs and 016 QALYs, respectively) for high-risk patients receiving optimal care for DFUs compared with usual care. Total cost savings for Australia were estimated at AUD 27 billion over 5 years. Probabilistic sensitivity analysis showed that optimal care always had a higher probability of costing less and generating more health benefits. This study provides important evidence to inform Australian policy decisions on the efficient use of health resources and supports the implementation of evidence-based optimal care in Australia. Furthermore, this information is of great importance for comparable developed countries that could reap similar benefits from investing in these well-known evidence-based strategies.

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