Choi S.B.,BK21 Plus Team |
Choi S.B.,Wonkwang University |
Bae G.-S.,Hanbang Body Fluid Research Center |
Jo I.-J.,BK21 Plus Team |
And 16 more authors.
Pancreas | Year: 2015
Methods: Acute pancreatitis was induced via intraperitoneal injection of cerulein (50 μg/kg) every hour for 6 times. In the LE, water extract (100, 250, or 500 mg/kg) was administered intraperitoneally 1 hour before the first injection of cerulein. Six hours after AP, blood, the pancreas, and the lung were harvested for further examination. In addition, pancreatic acinar cells were isolated using a collagenase method, and then, we investigated the acinar cell viability and cytokine productions.Objectives: We aimed to evaluate the anti-inflammatory and inhibitory effects of Lithospermum erythrorhizon (LE) on cerulein-induced acute pancreatitis (AP) in a mouse model.Results: Treatment with LE reduced pancreatic damage and AP-associated lung injury and attenuated the severity of AP, as evidenced by the reduction in neutrophil infiltration, serum amylase and lipase levels, trypsin activity, and proinflammatory cytokine expression. In addition, treatment with LE inhibited high mobility group box 1 expression in the pancreas during AP. In accordance with in vivo data, LE inhibited the cerulein-induced acinar cell death, cytokine productions, and high-mobility group box 1 expression. Furthermore, LE also inhibited the activation of p38 mitogenactivated protein kinases.Conclusions: These results suggest that LE plays a protective role during the development of AP by inhibiting the activation of p38. © 2014 Lippincott Williams & Wilkins.
Han Y.-H.,Wonkwang Oriental Medicines Research Institute |
Kee J.-Y.,Wonkwang Oriental Medicines Research Institute |
Park J.,Kyung Hee University |
Kim D.-S.,Wonkwang Oriental Medicines Research Institute |
And 8 more authors.
American Journal of Chinese Medicine | Year: 2016
Rutin, also called rutoside or quercetin-3-O-rutinoside and sophorin, is a glycoside between the flavonol quercetin and the disaccharide rutinose. Although many effects of rutin have been reported in vitro and in vivo, the anti-adipogenic effects of rutin have not been fully reported. The aim of this study was to confirm how rutin regulates adipocyte related factors. In this study, rutin decreased the expressions of adipogenesis-related genes, including peroxisome proliferators, activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), fatty acid synthase, adipocyte fatty acid-binding protein, and lipoprotein lipase in 3T3-L1 cells. Rutin also repressed the expression of lipin1, which is an upstream regulator that controls PPARγ and C/EBPα. In addition, when 3T3-L1 was transfected with lipin1 siRNA to block lipin1 function, rutin did not affect the expressions of PPARγ and C/EBPα. These results suggest that rutin has an anti-adipogenic effect that acts through the suppression of lipin1, as well as PPARγ and C/EBPα. © 2016 World Scientific Publishing Company.
Kim S.-J.,Daegu University |
Shin H.-J.,Wonkwang Oriental Medicines Research Institute |
Lee G.-H.,Kyung Hee University |
Kim D.-S.,Wonkwang Oriental Medicines Research Institute |
And 7 more authors.
Molecular Medicine Reports | Year: 2015
Ulcerative colitis (UC) is a type of inflammatory bowel disease and is considered a chronic gastrointestinal disorder. Igongsan (IGS) is a Korean herbal medicine, which has been used to treat digestive disorders. However, the ameliorative effect and molecular mechanisms of IGS in intestinal inflammation have not yet been studied in detail. The present study aimed to investigate the protective effects of IGS and its constituent, ergosterol, in a mouse model of dextran sulfate sodium (DSS)-induced colitis. Colitis was induced in mice by supplementing their drinking water with 5% (w/v) DSS for 7 days. The effects of IGS were then determined on DSS-induced clinical signs of colitis, including weight loss, colon shortening, diarrhea and obscure/gross bleeding. In addition, the effects of IGS were determined on the expression levels of inflammation-associated genes in the colon tissue of DSS-treated mice. The results of the present study demonstrated that mice treated with DSS exhibited marked clinical symptoms, including weight loss and reduced colon length. Treatment with IGS attenuated these symptoms and also suppressed the expression levels of tumor necrosis factor-α and interleukin-6, as well as the expression of cyclooxygenase-2 in the colon tissue of DSS-treated mice. IGS also reduced the activation of the transcription factor nuclear factor-κB p65 in the colon tissue of DSS-treated mice. In addition, ergosterol was shown to attenuate the DSS-induced clinical symptoms of colitis in mice. In conclusion, the present study provided experimental evidence that IGS may be a useful therapeutic drug for patients with UC.
Kwon S.-U.,Wonkwang Oriental Medicines Research Institute |
Jeon S.-B.,Muju Health Foods Co. |
Xin M.,Wonkwang Oriental Medicines Research Institute |
Kim J.-H.,Wonkwang Oriental Medicines Research Institute |
And 6 more authors.
Natural Product Sciences | Year: 2012
Hyperglycemia or high glucose (HG), is the hallmark of diabetes, known to induce oxidative stress, release of chemokines, and cytokines, which confer endothelial cell damage. On the other hand, microbial transformation of organic materials often leads to certain changes in their product structures which could enhance their biological activities. The aim of this study was to investigate the beneficial effects of fermented Gastrodia elata (FGE) in HG induced human umbilical vein endothelial cells (HUVECs) dysfunction. GE, fermented by Saccharomyces cerevisiae, which has an extensive history of safe use, exhibited higher phenolic compounds content than those of Gastrodia elata (GE). The HG-induced production of nitric oxide (NO) and interleukin-8 (IL-8) were significantly attenuated by FGE pretreatment to the cells, in a concentration dependent manner. In addition, FGE showed marked activity in free radical scavenging. These results suggest that FGE possesses beneficial effects in protecting against the oxidative stress, and inflammatory conditions in endothelial cells, caused by HG.