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Naaz M.,Ranchi University | Dutta A.,Ranchi University | Prasad A.,Womens College
International Journal of Pharma and Bio Sciences | Year: 2014

Tropical tasar silkworm, Antheraea mylitta Drury DABA-ecorace is commercially exploited in India for tasar silk production. Generally, its cocoons get infected with different microbes which causes loss to the silk industry. To curb these diseases antibiotics are being used but with partial success. In the present study, an antimicrobial peptide, Hen egg lysozyme (HEL) has been used and enzyme-based methods have been used to evaluate the impact on A. mylitta pupae. Interestingly, differences in protein concentration and fat body acid phosphatase (Acp) activity of treated pupae have been observed. Significant variation was observed in both profileof control and treated male pupae.The protein content of treated pupa has been found to be more than untreated but Acp activity profile have found decreased in treated pupae. It is expected that, based on protein content and enzyme-based method impact of antimicrobial peptideson A.mylitta pupae can be evaluated/observed and different antimicrobial peptides can be used as alternative to antibiotics resulting in increased silk yield.

Debnath P.,Tripura University | Chakraborty S.,Tripura University | Deb S.,Womens College | Nath J.,Tripura University | And 2 more authors.
Journal of Physical Chemistry C | Year: 2015

In the present Article, a reversible transition behavior from J-aggregates to excimer of an indocarbocyanine dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) in Langmuir-Blodgett (LB) films was reported. Surface pressure-area (π-A) isotherms, UV-vis, and fluorescence spectroscopies as well as atomic force microscopy (AFM) were used for characterizations of the films. π-A isotherms suggest a balance of interactions between DiI and fatty acids in the mixed monolayer at DiI mole fraction XDiI = 0.4, resulting in a stable and ideally mixed monolayer. It has been observed that pure DiI formed excimer in LB films, whereas both J aggregates and excimer were formed in LB films when DiI was mixed with long chain fatty acids, viz., stearic acid or arachidic acid. In fatty acid matrix at XDiI = 0.4, only J aggregates were formed in the LB films. This has been confirmed using deconvolution of spectroscopic results as well as using excitation spectroscopy. The coherent size of the J aggregate was found to be a maximum for the mixed film at the mole fraction 0.4 of DiI in fatty acid matrix. The J-aggregate domain in the LB film contains approximately (20 ± 5) coherent sizes. However, J aggregates were totally absent when DiI was mixed with cationic surfactant, polymer, or nanoclay. © 2015 American Chemical Society.

Hussain S.A.,Tripura University | Banik S.,Tripura University | Banik S.,Womens College | Chakraborty S.,Tripura University | Bhattacharjee D.,Tripura University
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2011

This work reports the adsorption kinetics of a highly fluorescent laser dye rhodamine B (RhB) in a preformed stearic acid (SA) Langmuir monolayer. The reaction kinetics was studied by surface pressure-time (π-t) curve at constant area and in situ fluorescence imaging microscopy (FIM). Increase in surface pressure (at constant area) with time as well as increase in surface coverage of monolayer film at air-water interface provide direct evidence for the interaction. ATR-FTIR spectra also supported the interaction and consequent complexation in the complex films. UV-vis absorption and Fluorescence spectra of the complex Langmuir-Blodgett (LB) films confirm the presence of RhB molecules in the complex films transferred onto solid substrates. The outcome of this work clearly shows successful incorporation of RhB molecules into SA matrix without changing the photophysical characteristics of the dye, thus making the dye material as LB compatible. © 2011 Elsevier B.V. All rights reserved.

Dold A.P.,University of Toronto | Zywiel M.G.,University of Toronto | Taylor D.W.,University of Toronto | Dwyer T.,Womens College | Theodoropoulos J.,University of Toronto
Clinical Journal of Sport Medicine | Year: 2014

OBJECTIVE:: Using systematic review methodology, we endeavored to answer the following questions concerning the treatment of osteochondral pathology: (1) what pathologies have been treated in vivo with the use of platelet-rich plasma (PRP); (2) what methods of PRP preparation and delivery have been reported; (3) what assessment tools and comparison group have been used to assess its effectiveness; and (4) what are the clinical outcomes of its use. DATA SOURCES:: A systematic literature search was performed of the OVID, EMBASE, and Evidence Based Medicine Reviews databases to identify all studies published up to October 2012 that assessed clinical outcomes of the use of PRP for the treatment of chondral and osteochondral pathology, excluding those including concomitant management of acute fractures or ligament reconstruction. DATA EXTRACTION:: The included studies were reviewed and the following data were extracted and tabulated: study authorsÊ year and journal, study design and level of evidence, pathology treated, methods of PRP preparation and delivery, and clinical outcome scores. DATA SYNTHESIS:: Ten studies were included in the final analysis. The majority of studies assessed the use of PRP in the treatment of degenerative osteoarthritis of the knee or hip (representing 570 of a total of 662 joints). The majority of patients were treated with intra-articular injections, whereas 2 studies used PRP as an adjunct to surgical treatment. Significant improvements in joint-specific clinical scores (7 of 8 studies), general health scores (4 of 4 studies), and pain scores (4 of 6 studies) compared with baseline were reported up to 6-month follow-up, but few studies provided longer-term data. No studies reported worse scores compared with baseline at final follow-up. Three of 4 comparative studies reported significantly better clinical and/or pain scores when compared with hyaluronic acid injections at similar follow-up times. CONCLUSIONS:: Currently, there is a paucity of data supporting the use of PRP for the management of focal traumatic osteochondral defects. There is limited evidence suggesting short-term clinical benefits with the use of PRP for symptomatic osteoarthritis of the knee, but the studies published to date are of poor quality and at high risk for bias. Further high-quality comparative studies with longer follow-up are needed to ascertain whether PRP is beneficial, either alone or as an adjunct to surgical procedures, in the management of articular cartilage pathology. © 2014 by the American Medical Society for Sports Medicine.

Bhattacharjee J.,Tripura University | Banik S.,Womens College | Hussain S.A.,Tripura University | Bhattacharjee D.,Tripura University
Chemical Physics Letters | Year: 2015

In the present communication, interaction of tetracationic porphyrin, 5,10,15,10-tetrakis (1-methyl-4-pyrindino) porphyrin tetra (p-toluenesulfonate) (TMPyP) with anionic nano clay platelets laponite has been studied in aqueous clay dispersion and Layer-by-Layer (LbL) self assembled film. Electrostatic adsorption of TMPyP molecules on clay platelets resulted in the flattenening of meso-substituent groups which led to the development of a new adsorbed band in the UV-vis absorption spectra. J-band was also formed due to overlapped organizations of organo-clay hybrid molecules in the LbL film leading to J-aggregates. Atomic force microscopic (AFM) studies gave visual evidence of this favoured organization in the monolayer LbL film. © 2015 Elsevier B.V. All rights reserved.

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