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Quetta, Pakistan

Faiz A.H.,Bio Resource Research Center | Abbas F.-I.,Bio Resource Research Center | Ali Z.,University of Punjab | Nadeem S.,Bio Resource Research Center | And 2 more authors.
Journal of Animal and Plant Sciences | Year: 2015

Distribution of the rodents in different areas of pothwar regions was recorded from January to October, 2014 using snap traps. A total of 179 rodents viz. B. bengalensis (n = 2), M. musculus (n = 50), M. booduga (n = 10), M. ameltada (n = 3), S. smurinus (n = 10), G. ellioti (n = 2), T. indica (n = 22), R. rattus (n = 50), R.norvegicus (n = 30) were captured during 468 trap nights with (trap success: 0.38). Simpson Index was (0.2), Shannon Winner Index (2.54), and Berger- Parker Dominance Index was (0.27). Averages of 3.3 rats per farm house were recorded with Rodent Index values (0.011). © 2015 Pakistan Agricultural Scientists Forum. All rights reserved.

Kiani M.Z.,PARC Institute of Advanced Studies in Agriculture | Sultan T.,Pakistan National Agricultural Research Center | Ali A.,Pakistan National Agricultural Research Center | Rizvi Z.F.,Women University
Pakistan Journal of Botany | Year: 2016

Salinity increase ethylene level in root rhizosphere. Plant growth promoting rhizobacteria having ACC deaminase have ability to mitigate the adverse effect of enhanced level of ethylene. Two field experiments were carried out at two different sites during 2013. The site1 had ECe 9.42 dS m-1 and SAR 20.75 (mmolc L-1)1/2, while site2 had ECe 7.51 dS m-1 and SAR 16.25 (mmolc L-1)1/2. Four PGPR strains (KS 44, KS 7, KS 41, KS 42) having ACC deaminase activity were selected for this study. Randomized completes block design (RCBD) was used and hybrid sunflower seeds (SMH-0917) were inoculated with these PGPR strains. The crop was harvested at maturity and data were recorded about plant height, shoot dry weight, head dry weight, number of seed head-1, 1000 seed weight, seed weight head-1 and seed yield kg ha-1. The data were statistically analyzed by using soft ware statistic 8.1. The average increase in grain yield (site 1 + site 2) was 110.07, 108.89, 49.09, 65.77 and 69.70% over control due to inoculation with KS 44, KS 7, KS 41, KS 42 and KS mix treatments respectively. On the basis of statistical analysis, the bacterial strains KS 44 and KS 7, were declared as most promising strains on both sites. Hence these two strains (KS 44 and KS 7) could be used to mitigate negative impact of salinity stress on sunflower. © 2016, Pakistan Botanical Society. All rights reserved.

Soomro A.N.,University of Sindh | Waryani B.,University of Sindh | Hiroshi S.,Kagoshima University | Baloch W.A.,University of Sindh | And 3 more authors.
Pakistan Journal of Zoology | Year: 2016

Diversity of freshwater shrimps was investigated along the continuum of Urabaru stream Kikaijima Island, Japan. Total five stations were sampled from May 2007 to December 2008 in Urabaru stream. Total twelve species including seven Atyid and five Palaemonid shrimps were observed during the study. Macrobrachium grandimanus and Atypsis spinipes are reported for first time in a stream of Kikaijima Island. Macrobrachium japonicus, Macrobrachium lar and Caridina typus bserved at all five stations. Higher Margalef diversity was recorded at stations 4 and 5 and lower at Stations 1, 2 and 3. Cluster analysis recorded higher similarity between Stations 4 and 5 at one cluster and Sations 1, 2 and 3 at other. Our results suggesting higher diversities at stations located near river mouth (4 and 5) and gradulay lower diversities at the stations located away from the river mouth (1, 2 and 3). Diversity of all the stations located along the continuum of Urabaru stream is mainly effected by amphidromy of the shrimps. Copyright 2016 Zoological Society of Pakistan.

Amanullah,Quaid-i-Azam University | Mirza B.,Quaid-i-Azam University | Rizvi Z.F.,Women University | Zia M.,Quaid-i-Azam University
Pakistan Journal of Botany | Year: 2016

Artemisinin and its derivatives are phytochemical constituents of genus Artemisia. Demand of these plant secondary metabolitesis increasing due to their immense therapeutic significance. Besides their established antimalarial role, recent studies have also disclosed their anticancer potentials. It has made imperative to develop new and efficient sources of these compounds. Inherent synthetic challenges give biological sources preference over chemical synthesis of artemisinin and its derivatives. Therefore, genetic improvement of plants and, rather less preferentially, microbes is focus of current research to gain increase productivity of these valuable drugs. This study has analyzed A. dubiaas potential source of artemisinin and its derivatives. Transformation of Artemisia dubia was carried out using A. tumefaciens strain LBA 4404 containing rolA gene constructed on pRB 29. Healthy and acclimatizable transgenic plants were produced using optimized concentrations of BAP and NAA. Previously acclimatized rol ABC transgenic plants were also In vitro regenerated for comparative analysis of artemisinin and its derivatives. PCR amplification of rolA gene was done to confirm the integration of T-DNA in transgenic plants.TLC analysis was performed to evaluate comparative production of artemisinin and derivatives in rolA and rol ABC transgenic A. dubia. It revealed that rolA transgenic plants contain comparable amounts of these metabolites. Both type oftransgenic plants manifested the enhancement of other uncharacterized compounds as well. Besides systematic optimization of In vitro regenerative protocol for Artemisia dubia, relative regeneration ability of rol transgenic and control plants was also assessed at four regenerative stages. It was observed that unlike control, rol transgenic plants showed best root induction only on combination of auxins and cytokines. It was concluded that rol genes transformation of plants is an efficient tool to enhance their secondary metabolites production. RolA transgenic A. dubiaare cultivable, viable and efficient source of artemisinin and its derivatives to meet their ever-growing demands. © 2016, Pakistan Botanical Society. All rights reserved.

Shabana,University of Punjab | Hasnain S.,University of Punjab | Hasnain S.,Women University
BioMed Research International | Year: 2015

Background/Objective. Obesity has become a global epidemic due to an increase in the number of obese individuals worldwide. There is little research in the field of obesity genetics in Pakistan. The aim of the current study was to analyze the association of common variants in Fat Mass and Obesity associated (FTO) gene with obesity in Pakistan, to find out the effect of the selected SNPs on anthropometric and biochemical traits, and to observe whether these variants act synergistically. Methods. Samples from 631 subjects were taken after informed consent and were used for serum parameters and genetic analysis. Lipid profile was determined, tetra-ARMS PCR was used for genotyping, and allele/genotype frequencies and genescore were calculated. Results. All FTO variants were associated with obesity, and some biochemical and anthropometric measures and had higher minor allele frequencies than those reported for Asian populations previously. The risk allele of each single nucleotide polymorphism resulted in an increase in BMI in a quantitative manner. Conclusion. Common forms of obesity are due to a combined net effect of many variants presented in same or different genes. The more the number of risk alleles present, the higher the risk and severity of obesity resulting from an increase in BMI. © 2015 Shabana and Shahida Hasnain.

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