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Kang Y.,Women and Infants Hospital of Zhengzhou
Energy Education Science and Technology Part A: Energy Science and Research | Year: 2014

Objective: To explore the correlation of infantile diarrhea with rotavirus infection and lactose intolerance and assist to differentiate the cause of infantile diarrhea. Methods: The stool samples of 144 children that age of under 3 years with diarrhea from our outpatients and inpatients were collected to do routine examination, bacterial culture, fecal rotavirus antigen detection and reducing sugar determination. Results: There were 103 (73. 6%) infants with rotavirus enteritis 90 (62.5%) infants with lactose intolerance, 75 infants with rotavirus infection complicated with lactose intolerance were 71. 1% of the infants with rotavirus infection. Conclusions: Rotavirus was the commonest pathogen of infant diarrhea in autumn and winter, and most of these patients with rotavirus enteritis had secondary lactose intolerance. © Sila Science. All rights reserved.


Li Y.,Zhengzhou University | Li Y.,Henan Institute for Food and Drug Control | Zhu J.-K.,Zhengzhou University | Feng H.,Women and Infants Hospital of Zhengzhou | And 2 more authors.
Fenxi Huaxue/ Chinese Journal of Analytical Chemistry | Year: 2015

Cellulose acetylsalicylate chiral stationary phase (ACSP) was synthesized by using cellulose and o-acetylsalicylryl chloride, and its enantioseparation ability was evaluated by high performance liquid chromatography (HPLC). Commercial Chiralcel OJ column was evaluated for comparison. The effects of mobile phase composition and double ester carbonyls of derivative on enantioseparation were investigated. The structure of the obtained derivative was characterized by infrared (IR) spectroscopy and thermogravimetric analysis. Hexane-isopropanol (90:10-80:20, V/V, 0.1% DEA or TFA) was selected by comparison of four mobile phases, namely hexane-isopropanol, hexane-ethanol, hexane-methanol-isopropanol and hexane-methanol-dichloroethane. Seven racemates of catecholamines and amides were used to evaluate its chiral recognition ability in normal phase elution mode, and the regularity and characteristics of the novel chiral stationary phase were explored. The chromatographic results showed that cellulose ACSP exhibited high enantioseparation ability for catecholamines and some racemates with amide group due to the hydrogen-bond, dipole interaction of carbonyls of acetylsalicylate and the π-π interaction with benzene ring, and the optimum amount of diethylamine or trifluoroacetic acid was 0.1%. Copyright © 2015, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Published by Elsevier Limited. All rights reserved.


Liu J.,Zhengzhou University | Liu Y.,Zhengzhou University | Wang W.,Zhengzhou University | Wang C.,Zhengzhou University | Che Y.,Women and Infants Hospital of Zhengzhou
Experimental and Therapeutic Medicine | Year: 2015

The main obstacle in the development of an effective tumor vaccine is the inherent ability of tumors to evade immune responses. Tumors often use common immune mechanisms and regulators to evade the immune system. The present study aimed to analyze the expression levels of indoleamine 2,3‑dioxygenase (IDO), programmed death‑ligand (PD‑L) 1, PD‑L2, B7‑H4, galectin‑1 and galectin‑3 in tissue samples from patients with endometrial carcinoma, in order to detect the immunosuppressive environment of endometrial carcinomas. The levels of IDO, PD‑L1, PD‑L2 and B7‑H4 were analyzed by immunohistochemical methods, and the levels of galectin‑1 and galectin‑3 in tumor lysates were determined using ELISA. PD‑L2 was expressed at low levels in the majority of tumor samples. IDO expression was detected in 38, 63 and 43% of primary endometrial carcinoma, recurrent endometrial carcinoma, and metastatic endometrial carcinoma specimens, respectively. Positive expression rates for PD‑L1 were 83% in primary endometrial carcinoma, 68% in recurrent endometrial carcinoma, and 100% in metastatic endometrial carcinoma, whereas B7‑H4 expression was detected in 100% of both primary endometrial carcinoma and recurrent endometrial carcinoma samples, and in 96% of metastatic endometrial carcinoma specimens. The expression levels of galectin‑1 and galectin‑3 were not significantly different between the normal and tumor specimens. The results of the present study suggest that the interaction between PD‑1/PD‑L1 and B7‑H4 may be a potential target for immune intervention in the treatment of endometrial carcinoma. Furthermore, the results may provide the basis for immunosuppressant therapy in the treatment of patients with uterine cancer. © 2015, Spandidos Publications. All rights reserved.


Zhang M.,Women and Infants Hospital of Zhengzhou | Xu Q.,Women and Infants Hospital of Zhengzhou | Yan S.,Women and Infants Hospital of Zhengzhou | Li Z.,Women and Infants Hospital of Zhengzhou | Jia X.,Jilin University
Oncology Reports | Year: 2016

MicroRNAs (miRNAs) play a pivotal role in cancer progression and development, representing novel therapeutic tools for cancer therapy. Forkhead box Q1 (FOXQ1) functions as an oncogene in various cancer types. However, the functional significance of FOXQ1 in cervical cancer remains unknown. In this study, we investigated the biological function of FOXQ1 in cervical cancer and tested whether or not FOXQ1 can be targeted and regulated by specific miRNAs. We found that FOXQ1 was highly expressed in cervical cancer cell lines. Knockdown of FOXQ1 by small interfering RNA (siRNA) significantly suppressed the proliferation and epithelial-mesenchymal transition (EMT) of cervical cancer cells. FOXQ1 was predicted as a target gene of microRNA-506 (miR-506), and this prediction was validated by dual-luciferase reporter assay. Quantitative real-time PCR and western blot analyses demonstrated that mRNA and protein expression was negatively regulated by MIR-506. The expression of MIR-506 was downregulated in cervical cancer tissues, and MIR-506 expression was inversely correlated with FOXQ1 expression in cervical cancer. The overexpression of MIR-506 dramatically suppressed the proliferation and EMT of cervical cancer cells that mimicked the suppression of FOXO1 siRNA. Furthermore, the restoration of FOXQ1 expression significantly reversed the inhibitory effect of MIR-506. Overall, our study demonstrated that MIR-506 inhibited the proliferation and EMT of cervical cancer cells by targeting FOXQ1 and provided evidence that the MIR-506/FOXQ1 axis plays an important role in the pathogenesis of cervical cancer, representing potential molecular targets for the development of anticancer agents for cervical cancer treatment.


Song Z.-S.,Zhengzhou University | Wu Y.,Women and Infants Hospital of Zhengzhou | Zhao H.-G.,Women and Infants Hospital of Zhengzhou | Liu C.-X.,Women and Infants Hospital of Zhengzhou | And 4 more authors.
Oncology Letters | Year: 2016

Polymorphisms in microRNA (miR) genes and their target sites are a distinct classification of variation in the human genome, which are rapidly being identified and investigated in human cancer. A polymorphism in the miR-196a-2 locus has demonstrated significant associations with various types of cancer, including lung, breast, esophageal and gastric tumors. However, miR-196a-2 has not been fully explored in ovarian cancer, which shares similar biological characteristics with other types of cancer. Therefore, the present study aimed to elucidate the association between a single nucleotide polymorphism (SNP) in the mature sequence of miR-196a-2 (rs11614913, T/C) and the clinical features of 479 Chinese patients with epithelial ovarian cancer (EOC). In addition, the biological significance of this polymorphism was investigated in the OVCAR3 ovarian cancer cell line. Risk association was evaluated in 479 cases of EOC patients and 431 controls. SNPs were analyzed by using polymerase chain reaction based restriction fragment length polymorphism assay. miR-196a expression was evaluated with reverse transcription polymerase chain reaction. The influence of miR-196a-2 rs11614913 T/C on EOC cell migration and invasion ability was further investigated in vitro. The results revealed significant differences in the homozygous CC genotype distribution in patients with EOC (n=479), compared with that of the control subjects (n=431; P=0.026). Analysis of the association between genotype and the risk of EOC revealed that individuals who carried the homozygous CC genotype were 1.34-fold more susceptible to EOC, compared with those carrying the wild-type TT and heterozygous CT genotypes [odds ratio, 1.34; 95% confidence interval, 1.04-2.17; P=0.023]. © Spandidos Publications 2015. All rights reserved.

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