Women and Infants Hospital of Zhengzhou

Zhengzhou, China

Women and Infants Hospital of Zhengzhou

Zhengzhou, China
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Zhong J.,Xinxiang Medical University | Liu Y.,Xinxiang Medical University | Xu Q.,Women and Infants Hospital of Zhengzhou | Yu J.,Xinxiang Medical University | Zhang M.,Jilin University
Biochemical and Biophysical Research Communications | Year: 2017

Disheveled-Axin domain containing 1 (DIXDC1) is involved in the development and progression of multiple cancers. However, the function significance of DIXDC1 in prostate cancer remains unclear. In this study, we investigated the function of DIXDC1 in prostate cancer and the regulation of DIXDC1 by microRNAs (miRNAs). We found that DIXDC1 was highly expressed in prostate cancer cells. Knockdown of DIXDC1 by small interfering RNAs markedly suppressed proliferation, invasion and Wnt signaling in prostate cancer cells. DIXDC1 was identified as a target gene of miR-1271 by bioinformatics analysis, dual-luciferase reporter assay, real-time quantitative polymerase chain reaction and Western blot analysis. Furthermore, DIXDC1 expression was inversely correlated with miR-1271 expression in prostate cancer tissues. The overexpression of miR-1271 significantly inhibited proliferation, invasion and Wnt signaling in prostate cancer cells. However, the inhibition of miR-1271 exhibits the opposite effects. Moreover, the overexpression of DIXDC1 significantly reversed the inhibitory effects of miR-1271 overexpression. Taken together, our results suggest that DIXDC1 plays an important role in regulating prostate cancer cell proliferation and invasion. Targeting DIXDC1 by miR-1271 may be a promising therapeutic strategy for prostate cancer. © 2017 Elsevier Inc.


Zhang X.,Women and Infants Hospital of Zhengzhou | Jiang Y.,Xinxiang Medical University | Liu Y.,Women and Infants Hospital of Zhengzhou | Wang L.,Zhengzhou University
International Journal of Clinical and Experimental Pathology | Year: 2017

The pathogenesis of preeclampsia involves many environmental and genetic factors. An excessive inflammatory response during pregnancy could cause an imbalance in the immune system, and thus contributes to the development of preeclampsia. Inflammatory cytokines produced by T-helper 2 cells, such as IL-4, contribute to inhibiting cellular immunity and inducing the placental growth. Two common genetic polymorphisms were observed in IL-4, including C-590T and C+33T. We performed a study to investigate the association between IL-4 C-590T and C+33T polymorphisms and development of preeclampsia in a Chinese pregnant women. We conducted a case-control study that included 162 pregnant women with preeclampsia and 266 healthy controls. The genotyping of the IL-4 C-590T and C+33T was carried out by a method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). By logistic regression analysis, the CC genotype of IL-4 C-590T was significant associated with a reduced risk of preeclampsia when compared with the GG genotype, and the adjusted OR (95% CI) was 0.39 (0.15-0.90). In dominant model, we observed that the GC+CC genotype was correlated with a decreased risk of preeclampsia in comparison to the GG genotype (OR=0.59, 95% CI=0.35-0.97). In recessive model, we found that the CC genotype exposed a lower risk of preeclampsia than the GG+GC genotype (OR=0.41, 95% CI=0.16-0.94). However, no significant association was found between L-4 C+33T polymorphism and susceptibility to preeclampsia. In conclusion, our study suggests that IL-4 C-590T polymorphism could be a risk factor for preeclampsia.


Ge H.-X.,Women and Infants Hospital of Zhengzhou
European review for medical and pharmacological sciences | Year: 2017

OBJECTIVE: We aimed to investigate the effect of Embosphere microsphere artery embolization on the serum level of vascular endothelial growth factor (VEGF) in patients with uterine fibroid.PATIENTS AND METHODS: From March 2014 to December 2015, 128 women in child-bearing age with uterine intramural fibroids were enrolled in the patient group. At the same time, 128 healthy cases in child-bearing age were randomly selected and enrolled in the control group. Enzyme-linked immunosorbent assay was used to measure the serum level of VEGF, and immunohistochemical staining method was used to study the expression of VEGF in the uterine fibroids. Embosphere microsphere artery embolization surgery was performed on cases in the patient group.RESULTS: The serum level of VEGF in the patient group was significantly higher than that of the control group. Immunohistochemical staining results showed that in the control group, VEGF expression level in uterine fibroid tissue was significantly higher. Compared with before the treatment, tumor diameter in the patient group reduced significantly 3 months after the treatment. Erythrocyte count, hemoglobin, and menstrual blood volume increased significantly 6 months after treatment.CONCLUSIONS: Serum VEGF level can be considered as a marker for uterine fibroid, and by using VEGF as a marker we can increase the probability of early diagnosis. We showed that, compared with hysterectomy, Embosphere microsphere embolization had an evident advantage and might be an excellent candidate to replace hysterectomy.


Huang N.-N.,Women and Infants Hospital of Zhengzhou
European review for medical and pharmacological sciences | Year: 2016

OBJECTIVE: This study aims to investigate the risk factors for neonatal nosocomial enteric infection (NNEI) and the effect of intervention with BIFICO.PATIENTS AND METHODS: Between May 2013 and June 2015, 215 neonates admitted to our institution were randomly divided into the study group and the control group, 47 for each group. Patients in the study group were treated for primary diseases combined with the oral admission of BIFICO, whereas patients in the control group were treated for primary disease alone. Statistical analysis was performed to obtain the occurrence of enteric infection and univariate, as well as multivariate analysis of clinical data, were performed to investigate the underlying risk factors.RESULTS: Univariate and multivariate analysis of variance showed that gestational age, birth weight, length of hospital stay, invasive procedures and underlying diseases were risk factors affecting NNEI. The occurrence of NNEI in the study group was significantly lower than that in the control group [17.02% (8/47) vs. 29.79% (14/47), X2 = 19.394, p = 0.004].CONCLUSIONS: Preterm infant, low-birth-weight infant, length of hospital stay, invasive procedures and comorbidity are independent risk factors for NNEI. Prophylactic therapy with BIFICO can effectively decrease the occurrence of infections and can be widely used in clinical practice.


Gao X.,Nanjing Southeast University | Zhang S.,Nanjing Southeast University | Qiao X.,Women and Infants Hospital of Zhengzhou | Yao Y.,Women and Infants Hospital of Zhengzhou | And 4 more authors.
Cancer Biomarkers | Year: 2014

BACKGROUND: Associations between cytotoxic T lymphocyte antigen-4 (CTLA-4) +49A/G polymorphism and cancer risk are inconclusive. We performed this meta-analysis to derive a more precise estimation of the relationship. METHODS: A comprehensive literature search was performed using electronic databases. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of association. RESULTS: A total of 16,358 cases and 19,737 controls from 46 studies were included. Overall, significant association between CTLA-4 +49A/G polymorphism and cancer risk was observed in all genetic models (G vs. A: OR=0.88, 95%CI=0.83-0.93, P-H=0.000; GA vs. AA: OR=0.87, 95%CI=0.79-0.97, P-H=0.000; GG vs. AA: OR=0.75, 95%CI= 0.65-0.86, P-H=0.000; GG vs. GA+AA: OR=0.84, 95%CI=0.79-0.91, P-H=0.001; GG+GA vs. AA: OR=0.83, 95%CI=0.74-0.92, P-H=0.000). Stratified analysis by cancer type revealed that the CTLA-4+49A/G polymorphism is associated with the decreased risk of cervical cancer, breast cancer, lung cancer, HCC. Further subgroup analysis by ethnicity indicated that there was a statistically decreased cancer risk in Asian population. CONCLUSION: This meta-analysis suggests that CTLA-4+49A/G polymorphism is associated with cancer risk, especially in Asian population. © 2014 - IOS Press and the authors. All rights reserved.


PubMed | Jilin University, Zhengzhou Central Hospital and Women and Infants Hospital of Zhengzhou
Type: Journal Article | Journal: Oncology reports | Year: 2016

MicroRNAs (miRNAs) play a pivotal role in cancer progression and development, representing novel therapeutic tools for cancer therapy. Forkhead box Q1 (FOXQ1) functions as an oncogene in various cancer types. However, the functional significance of FOXQ1 in cervical cancer remains unknown. In this study, we investigated the biological function of FOXQ1 in cervical cancer and tested whether or not FOXQ1 can be targeted and regulated by specific miRNAs. We found that FOXQ1 was highly expressed in cervical cancer cell lines. Knockdown of FOXQ1 by small interfering RNA (siRNA) significantly suppressed the proliferation and epithelial-mesenchymal transition (EMT) of cervical cancer cells. FOXQ1 was predicted as a target gene of microRNA-506 (miR-506), and this prediction was validated by dual-luciferase reporter assay. Quantitative real-time PCR and western blot analyses demonstrated that mRNA and protein expression was negatively regulated by miR-506. The expression of miR-506 was downregulated in cervical cancer tissues, and miR-506 expression was inversely correlated with FOXQ1 expression in cervical cancer. The overexpression of miR-506 dramatically suppressed the proliferation and EMT of cervical cancer cells that mimicked the suppression of FOXO1 siRNA. Furthermore, the restoration of FOXQ1 expression significantly reversed the inhibitory effect of miR-506. Overall, our study demonstrated that miR-506 inhibited the proliferation and EMT of cervical cancer cells by targeting FOXQ1 and provided evidence that the miR-506/FOXQ1 axis plays an important role in the pathogenesis of cervical cancer, representing potential molecular targets for the development of anticancer agents for cervical cancer treatment.


Ren H.,Childrens Hospital Of Zhengzhou | Shi X.,Women and Infants Hospital of Zhengzhou | Li Y.,Childrens Hospital Of Zhengzhou
Experimental and Therapeutic Medicine | Year: 2016

Human laryngeal papilloma (LP) is a human papillomavirus-induced hyperplastic tumor of the respiratory tract, which is characterized by rapid growth and apoptosis resistance. Isoflurane (ISO) inhibits proliferation and elicits apoptosis in cancer cells. The results of the present study found that the mRNA and protein levels of cyclooxygenase-2 (COX2) were higher in LP tissues than in normal laryngeal samples, and prostaglandin E2 (PGE2) production was increased in LP cells, as determined by quantitative polymerase chain reaction, western blot and radioimmunoassay analyses. Notably, the increase in COX2 and PGE2 levels was significantly abrogated in the ISO-treated LP cells. The inhibitory effects of ISO on COX2 expression and activity depended on the inactivation of p38 mitogen-activated protein kinase (MAPK) in LP cells. By inhibiting the COX2 activity of LP cells, ISO treatment markedly suppressed cell viability and proliferation, as determined using Cell Counting Kit-8, flow cytometry and 5-ethynyl-20-deoxyuridine incorporation assays. Furthermore, ISO treatment promoted cell apoptosis, as demonstrated by flow cytometry, nucleosomal fragmentation and caspase-3 activity assays. Collectively, the present results suggest that COX2 is critical in the progression of LP, and ISO is a potential agent for LP therapy by impeding p38 MAPK/COX2 signaling. © 2016, Spandidos Publications. All rights reserved.


PubMed | Childrens Hospital of Zhengzhou, Henan University of Traditional Chinese Medicine, Women and Infants Hospital of Zhengzhou and Henan Eye Hospital
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2017

The effect of glucocorticoid on cytokines Toll-like receptor (TLR)9 and TLR7 in peripheral blood of patients with uveitis was explored. Forty-six patients with uveitis admitted to our hospital from April 2014 to April 2015 were selected as the research observational group. Thirty-five able-bodied individuals in the same period were selected as the control group. To treat uveitis, the observational group was injected with glucocorticoid (1-2 mg/kg/day) daily, while the control group did not receive any treatment. The quantity of expression of peripheral blood cytokines TLR9 and TLR7 were detected by the methods of fluorescence quantitative PCR, enzyme-linked immunosorbent assay and western blotting. The content of peripheral blood TLR9 and TLR7 (0.210.01, 0.190.01) decreased significantly (P<0.05) in observational group after glucocorticoid treatment. Compared with data of control group (0.210.01, 0.190.01), TLR9 and TLR7 content in peripheral blood after glucocorticoid treatment on the patients with uveitis from observation group (0.190.01, 0.170.01) did not show any significant difference, for correlation between TLR9 and TLR7 in observation group before and after treatment. It was observed that the cytokine content of TLR9 was associated with TLR7 positively (r=0.653, P=0.012). In conclusion, glucocorticoid can improve uveitis by reducing the content of cytokines TLR9 and TLR7 in peripheral blood.


PubMed | Women and Infants Hospital of Zhengzhou and Zhengzhou University
Type: Journal Article | Journal: Pakistan journal of medical sciences | Year: 2017

To explore the effects of letrozole (LE) in combination with low-dose intramuscular injection of human menopausal gonadotropin (HMG) on the ovulation induction and pregnancy of patients with polycystic ovary syndrome (PCOS).A total of 156 patients with PCOS infertility were randomly divided into an LE group, a clomiphene citrate (CC) group and an LE + HMG group (n= 52). LE and CC were orally taken according to the prescribed dosage on the 3rd-5th days of menstruation respectively, and 75 IU HMG was given through intramuscular injection. The ovulation induction parameters and pregnancy outcomes were observed.The number of ovulation cycle of LE + HMG group was significantly higher than that of LE group (The regimen using LE in combination with low-dose intramuscular injection of HMG has satisfactory therapeutic effects on ovulation induction, short medication cycle and high clinical pregnancy rate, which is promising for treating patients with PCOS infertility.


Kang Y.,Women and Infants Hospital of Zhengzhou
Energy Education Science and Technology Part A: Energy Science and Research | Year: 2014

Objective: To explore the correlation of infantile diarrhea with rotavirus infection and lactose intolerance and assist to differentiate the cause of infantile diarrhea. Methods: The stool samples of 144 children that age of under 3 years with diarrhea from our outpatients and inpatients were collected to do routine examination, bacterial culture, fecal rotavirus antigen detection and reducing sugar determination. Results: There were 103 (73. 6%) infants with rotavirus enteritis 90 (62.5%) infants with lactose intolerance, 75 infants with rotavirus infection complicated with lactose intolerance were 71. 1% of the infants with rotavirus infection. Conclusions: Rotavirus was the commonest pathogen of infant diarrhea in autumn and winter, and most of these patients with rotavirus enteritis had secondary lactose intolerance. © Sila Science. All rights reserved.

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