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Nishi-Tokyo-shi, Japan

Okabe A.,Hiroshima University | Hiramatsu R.,Hiroshima University | Umehara T.,Hiroshima University | Fujita Y.,Women | Shimada M.,Hiroshima University
Journal of Mammalian Ova Research | Year: 2014

Morphological changes are observed during the luteinization process of granulosa cells, however mechanisms and the roles are remained unclear. In the present study, we demonstrated that Sema3C is expressed in granulosa cells and then secreted to extracellular matrix accumulated within cumulus cell and granulosa cell layers. The expression is dependent on EGF-like factors (amphiregulin and neuregulin 1) and EGF receptor dependent manner. The knockdown of Sema3c not only significantly decreased the surface area of the granulosa cells, but also significantly suppressed the cell migration of granulosa cells cultured with EGF like factors. The phosphorylation of focal adhesion kinase (FAK) was dramatically suppressed by transfection with Sema3c siRNA, with an increasing level of phosphorylated ROCK, indicating that Sema3C regulated actin remodeling in a FAK signaling pathway-dependent manner. Moreover, the expression levels of genes involved in progesterone production, Star, Cyp11a1 were significantly decreased in Sema3c siRNA-transfected granulosa cells as compared with control siRNA transfection. From these results, we conclude that Sema3C is secreted and then accumulated within granulosa cell layers and cumulus cell layers. The FAK pathway is activated by Sema3C and induces cell migration and enlarges the area of granulosa cells. These morphological changes are required for luteinization (production of progesterone) of granulosa cells. ©2014 Japan Society for Ova Research.

Priven M.,Brigham and Womens Hospital | Baum J.,Women | Vieira E.,Simmons College | Fung T.,Simmons College | Herbold N.,Simmons College
Journal of the Academy of Nutrition and Dietetics | Year: 2015

Background: Given the rapid rise of free-from products available in the marketplace (especially gluten-free), more research is needed to understand how these products influence consumer perceptions of healthfulness. Objective: To determine whether perceptions of healthfulness can be generated about free-from products in the absence of risk information. Design: A survey was administered to 256 adults. Two picture-based food product questions evaluated which products consumers perceived to be healthier. One free-from designation was fabricated (MUI-free), whereas gluten-free was used as the comparison designation. For each question, participants chose which product they thought was healthier (free-from, conventional, or equally healthy). Statistical analyses: A χ2 test was run to assess the difference between responses to picture-based food product questions. Multinomial regression assessed variance in responses attributable to participant demographic characteristics. Results: Among the respondents, 21.9% selected the MUI-free product as healthier, whereas 25.5% selected the gluten-free product as healthier. Frequency data showed that a significant number of participants chose both free-from products as healthier than the conventional products (P<0.001). Regression analysis found that individuals who identified as gluten intolerant or unsure of a gluten intolerance were significantly more likely than other participants to choose the free-from product as healthier compared with choosing "equally healthy" (P=0.040). Hispanics and those with an associate's degree or vocational training were significantly more likely than their referent groups (whites and those with a doctoral degree, respectively) to choose the free-from product as healthier compared with choosing "equally healthy" (P=0.022 and 0.034, respectively). Finally, African Americans were more likely than whites to choose the conventional product as healthier compared with choosing "equally healthy" (P=0.016). Conclusions: Frequency data demonstrated that free-from products can generate perceptions of healthfulness in the absence of risk information. Self-reported intolerance data suggest that individuals with a heightened concern about the risks associated with gluten may perceive the larger category of free-from products as more healthful. In addition, ethnicity and education level appear to play a role in free-from product perception. © 2015 Academy of Nutrition and Dietetics.

Vaaler M.L.,Office of Program Decision Support | Stagg J.,Office of Program Decision Support | Parks S.E.,Office of Program Decision Support | Erickson T.,Women | Castrucci B.C.,Office of Program Decision Support
Journal of Nutrition Education and Behavior | Year: 2010

Objective: This study explored the influence of demographic characteristics on attitudes toward the benefits of breast-feeding, approval of public breast-feeding, and the use of infant formula. Additionally, the study examined whether attitudes were related to infant feeding practices among mothers enrolled in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) in Texas. Design: This study used a cross-sectional design. Setting: Participants completed questionnaires at WIC clinic sites across Texas. Participants: Mothers of young children who were receiving WIC benefits. Main Outcome Measure(s): Attitudes toward the benefits of breast-feeding, attitudes toward public breast-feeding, attitudes toward infant formula, and the choice of infant feeding practice. Analysis: Descriptive statistics, multivariate ordinary least squares regression, and multinomial logistic regression. Results: A key finding was that many Hispanic mothers held favorable attitudes toward both breast milk and infant formula. Younger and less educated mothers were least likely to agree with the benefits of breast-feeding. Mothers with positive attitudes toward the benefits of breast-feeding were likely to exclusively breastfeed and use both formula and breast milk. Conclusions and Implications: Attitudes toward breast-feeding, public breast-feeding, and infant formula and their influence on breast-feeding behavior should inform the curriculum of breast-feeding promotion programs.

Haider S.J.,Michigan State University | Chang L.V.,University of Cincinnati | Bolton T.A.,Women | Gold J.G.,Tower B B | Olson B.H.,University of Wisconsin - Madison
Health Services Research | Year: 2014

Study Design Quasi-random enrollment due to the excess demand for PC breastfeeding support services allowed us to compare the infants of women who requested services and were enrolled in the program (the treatment group, N = 274) to the infants of women who requested services and were not enrolled (the control group, N = 572). Data were analyzed using regression.Principal Findings The PC program increased the fraction breastfeeding at birth by 19.3 percent and breastfeeding duration by 2.84 weeks. Program participation also reduced the fraction of infants with gastrointestinal disorders by a statistically significant 7.9 percent. The program, if anything, increased the overall health care utilization.Conclusions This Michigan PC breastfeeding support program resulted in improvements in breastfeeding and infant health outcomes as measured by the diagnosis of ailments while increasing health care utilization.Objective To estimate the causal effect of a Michigan peer counselor (PC) breastfeeding support program for low-income women on infant health outcomes.Data Sources Program referral forms, program forms (enrollment, birth, and exit data), and state administrative data from the Women Infants and Children program, Medicaid, and Vital Records. © Health Research and Educational Trust.

Zhu J.-F.,Linyi Municipal Hospital | Zhan W.-J.,Linyi Municipal Hospital | Wang L.-H.,Shandong University | Wang Y.-H.,Women
Tropical Journal of Pharmaceutical Research | Year: 2015

This is a case study of five isolated orbital nerve inflammatory pseudo tumor cases presenting with protrusion and visual acuity of the right eye. Optic disk edema was observed by ophthalmoscopy. Plain and contrast-enhanced magnetic resonance imaging (MRI) were used to examine the orbital fat and enlargement of the right optic nerve sheath along with orbital magnetic resonance imaging diagnostic test. The visual acuity (VA) of all the admitted patients was 1.5/20. All the patients reported attacks of retrobulbar pain and severe headache at fairly regular intervals. The patients on clinical examination were found to have protrusion, reduced visual acuity of the right eye with an ipsilateral afferent papillary defect. There was no motility of the right eye and the exophthalmos of the right part was 27 mm. On examination, patients were diagnosed to have idiopathic orbital inflammation and received steroid therapy for 6 months. hereafter, patients received surgical treatment to remove the superior wall of the orbit followed by cortisone therapy for a further 6 months. The follow-up examination of the patients for one year revealed a gradual improvement in the vision of all the patients. The VA of the right eye for all the patients was 6/20, and the exophthalmos was now around 19 mm. Thus, a dramatic response to surgery and steroid treatment was observed in all the patients. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.

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