Lakhmale S.,Dr V J D Gramin Ayurved College |
Acharya R.N.,Gujarat Ayurved University |
Chavan S.S.,Wockhardt Research Center |
Ashok B.K.,Himalaya |
Ravishankar B.,Center for Research in Ayurveda & Allied science
Indian Journal of Natural Products and Resources | Year: 2016
Symphorema polyandrum Wight (Family Verbenaceae), an ethnomedicinal plant, is reported for its use in the management of snake bite by the tribal people of Odisha. In this study, the efficacy of S. polyandrum was evaluated for its antivenom activity. Seed powder 360 mg/kg was given to envenomed albino rats with sub-lethal dose of Naja naja venom (0.5 mg/kg), in normal saline by intraperitonial injection. Biochemical parameters like serum cholesterol, serum triglyceride, HDL cholesterol, serum total protein, albumin, globulin, alkaline phosphatase, blood sugar, SGPT and in haematological investigation only the total count was found significantly reversing the effect of venom between venom control and test drug group. The study also showed that the envenomation of rats led to increase in lipid peroxidation in all the three tissues. Administration of the test drug to venomised rat significantly decreased the lipid peroxidation in liver and heart. Further, it also enhanced the antioxidant activity through enzyme catalase in liver and heart. © 2016, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.
Chudiwal S.S.,Wockhardt Research Center
Drug Development and Industrial Pharmacy | Year: 2016
The objective of this study was to provide quality by design (QbD) approach for development of suspension type nasal spray products. Quality target product profile (QTPP) of test product budesonide nasal suspension (B-NS) was defined and critical quality attributes (CQAs) were identified. Critical formulation, process and delivery device variables were recognized. Risk assessment was performed by using failure mode and effect analysis (FMEA) methodology. Selected variables were further assessed using a Plackett Burman screening study. A response surface design consisting of the critical factors was used to study the interactions between the study variables. Formulation variable X2: median particle size of budesonide (D50) (µ) has strikingly influenced dissolution (%) (Y1), while D50 droplet size distribution (µm) (Y2) was significantly impacted by formulation variable X1: Avicel RC 591 (%) and process variable X4: homogenization speed (rpm). A design space plot within which the CQAs remained unchanged was established at lab scale. A comprehensive approach for development of B-NS product based on the QbD methodology has been demonstrated. The accuracy and robustness of the model were confirmed by comparability of the predicted value generated by model with the observed value. © 2016 Informa UK Limited, trading as Taylor & Francis Group
Upadhyay K.,Gujarat Narmada Valley Fertilizers Company Ltd |
Manvar A.,Saurashtra University |
Manvar A.,University College Dublin |
Rawal K.,Alembic Pharmaceuticals Ltd |
And 3 more authors.
Chemical Biology and Drug Design | Year: 2012
Tuberculosis caused by Mycobacterium tuberculosis remains a leading cause of mortality worldwide into 21st century. In continuation with our anti-tuberculosis research programme, in this work, we have prepared molecularly diverse coumarins clubbed with benzothiazepines as well as its aza-analogues-benzodiazepines by molecular hybridization. The resulting compounds were screened for their M. tuberculosis activity against H37Rv strains using microplate alamar blue assay. Among the designed diversity, the compounds 5k, 5n and 5o were found significantly active in primary anti-tuberculosis assay at minimum inhibitory concentration <6.25μm. Moreover, the IC50 values of 5k and 5o in level-2 screening were observed as >10μg/mL and 3.63μg/mL, respectively. Design and synthesis of more focused library and its three-dimensional quantitative structure activity relationship analysis are underway. In the present work, various coumarins clubbed with benzo(thi)diazepines were evaluated for their M. tuberculosis activity against H37Rv strains using MABA assay. The IC50 values of two analogs (compounds 5k and 5o) in level-2 screening were observed as >10μg/mL and 3.63μg/mL respectively. © 2012 John Wiley & Sons A/S.
Rane V.P.,Dr. Babasaheb Ambedkar Marathwada University |
Rane V.P.,Wockhardt Research Center |
Patil K.R.,Dr. Babasaheb Ambedkar Marathwada University |
Patil K.R.,Wockhardt Research Center |
And 3 more authors.
Journal of Chromatographic Science | Year: 2010
A simple and precise stability-indicating liquid chromatography method is developed and validated for the quantitative simultaneous estimation of irbesartan (IRB) and hydrochlorothiazide (HCTZ) in combined pharmaceutical dosage form. A chromatographic separation of the two drugs was achieved with an Ace5 C18 25-cm analytical column using buffer-acetonitrile (70:30 v/v). The buffer used in mobile phase contains 50 mM ammonium acetate pH adjusted 5.5 with acetic acid. The instrumental settings are flow rate of 1.5 mL/min, columntemperature at 30°C, and detector wavelength of 235 nm using a photodiode array detector. IRB, HCTZ, and their combination drug products were exposed to thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. Peak homogeneity data of IRB and HCTZis obtained using photodiode array detector. In the stressed sample chromatograms, it demonstrated the specificity of the assay method for their estimation in presence of degradation products. The described method shows excellent linearity over a range of 10-200 μg/mL for IRB and 5-100 μg/mL for HCTZ. Methylparaben was used as internal standard. The correlation coefficient for IRB and HCTZ are 0.998 and 0.999. The mean recovery values for IRB and HCTZ ranged from 100.45% to 101.25%. The limit of detection for IRB and HCTZ were 0.019 and 0.023 μg/mL, respectively, and the limit of quantification were 0.053 and 0.070 μg/mL, respectively. The proposed method was suitable for quantitative determination and stability study of IRB and HCTZ in pharmaceutical preparations and also can be used in the quality control of bulk manufacturing and pharmaceutical dosage forms.
PubMed | Dr. Babasaheb Ambedkar Marathwada University and Wockhardt Research Center
Type: | Journal: AAPS PharmSciTech | Year: 2016
Current endeavor was aimed towards monitoring percent weight build-up during functional coating process on drug-layered pellets. Near-infrared (NIR) spectroscopy is an emerging process analytical technology (PAT) tool which was employed here within quality by design (QbD) framework. Samples were withdrawn after spraying every 15-Kg cellulosic coating material during Wurster coating process of drug-loaded pellets. NIR spectra of these samples were acquired using cup spinner assembly of Thermoscientific Antaris II, followed by multivariate analysis using partial least squares (PLS) calibration model. PLS model was built by selecting various absorption regions of NIR spectra for Ethyl cellulose, drug and correlating the absorption values with actual percent weight build up determined by HPLC. The spectral regions of 8971.04 to 8250.77cm
Nikalje A.P.G.,P.A. College |
Shaikh S.I.,P.A. College |
Mulay A.,P.A. College |
Khan F.A.K.,P.A. College |
And 2 more authors.
Archiv der Pharmazie | Year: 2014
Two series of novel indolyl thiazolidin-4-one derivatives 4a-j and 5a-j were obtained by an ecofriendly synthetic protocol by treating a mixture of Schiff's bases (0.01 mol) with thioglycolic acid or thiolactic acid (0.01 mol) and anhydrous zinc chloride in catalytic amount in DMF as solvent under ultrasound irradiation, using an ultrasound synthesizer with a synthetic solid probe. The structures of the synthesized compounds were confirmed by IR, 1H NMR, 13C NMR, MS, and elemental analysis. The anticonvulsant activity and neurotoxicity of the newly synthesized compounds were established by MES and sc-PTZ model and by rotarod test, respectively, in vivo using mouse models. The actophotometer was used for the screening of behavioral activity. The compounds exhibited promising anticonvulsant activity; especially, the compounds showed maximum protection in the MES model at a dose of 100 mg/kg. Further, docking studies of the synthesized compounds were performed against the sodium channel receptor and showed good binding interactions with the receptor. A computational study was carried out to highlight the pharmacophore distance mapping, log p determination, and pharmacokinetic parameters. Novel indolyl thiazolidin-4-ones were designed and evaluated for their anticonvulsant and behavioral activities. Molecular docking studies, distance mapping, and prediction of the ADME properties were performed. 3-(2-Hydroxyphenyl)-2-(1H-indol-3-yl)thiazolidin-4-one at 100 mg/kg showed protection in the MES and sc-PTZ models and was found to decrease the behavioral activity in mice when compared to diazepam. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Wockhardt Research Center | Date: 2015-03-03
The invention relates to self emulsifying drug delivery system based compositions of rhein or diacerein, or salts or esters or prodrugs thereof which are bioequivalent to a 50 mg diacerein formulation marketed under the trade name Art 50. The compositions exhibit no variability in fed and fasted state conditions. The compositions also result in significant reduction in side effects such as, soft stools effects as compared to Art 50. The invention also relates to methods for preparing such compositions.
Wockhardt Research Center | Date: 2014-04-15
The present invention relates to stable pharmaceutical compositions comprising entacapone, levodopa and carbidopa, or pharmaceutically acceptable salts or hydrates thereof. The invention also relates to processes for the preparation of such compositions.
Wockhardt Research Center | Date: 2014-08-27
The invention relates to pharmaceutical compositions comprising unmicronized fenofibrate in admixture with a wetting agent and one or more pharmaceutically acceptable excipients, wherein the admixture is not comicronized before processing. The invention also relates to processes for the preparation of such compositions.
Wockhardt Research Center | Date: 2014-04-05
The invention relates to pharmaceutical compositions comprising rhein or diacerein or salts or esters or prodrugs thereof, optionally with one or more pharmaceutically acceptable excipients. The invention also relates to the methods for preparing such compositions.