Wits Advanced Drug Delivery Platform Research Unit

Parktown, South Africa

Wits Advanced Drug Delivery Platform Research Unit

Parktown, South Africa
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Adeyemi S.A.,Wits Advanced Drug Delivery Platform Research Unit | Choonara Y.E.,Wits Advanced Drug Delivery Platform Research Unit | Kumar P.,Wits Advanced Drug Delivery Platform Research Unit | Du Toit L.C.,Wits Advanced Drug Delivery Platform Research Unit | Pillay V.,Wits Advanced Drug Delivery Platform Research Unit
Journal of Nanomaterials | Year: 2017

The aim of this study is to effectively enhance antitumor activities of endostatin by preparing polymeric nanocarriers. NMR and FT-IR spectra confirmed the successful grafting of the CHT-g-PEI and CHT-g-PEI-PEG-NH2 conjugates. SEM micrographs confirmed the shape of endostatin-loaded nanoparticles to be spherical while both TEM and zeta size results showed nanoparticle's average size to be 100.6 nm having a positively charged surface with zeta potential of 7.95 mV. The concentrations of CHT and TPP as well as the changing pH conditions account for the increased swelling pattern of endostatin-loaded nanoparticles and influenced endostatin release in vitro. PEI increased the overall amine protonation while PEG aggravated endostatin encapsulation and release. Nanoparticles swell and release endostatin at acidic tumor pH of 6.8 compared to physiological pH of 7.4. The native CHT-g-PEI-PEG-NH2 conjugate showed high cytocompatibility above 80% cell viability across tested formulations. Endostatin-loaded nanoparticles showed a significant reduction in cell viability across tested formulations, with 5.32% cell death at 125 μg/mL and 13.36% at 250 μg/mL following 24 hours' incubation period. Interestingly, more than a fourfold (61.68%) increment in cytotoxicity was observed at nanoparticle concentration of 1000 μg/mL. It was concluded that CHT-g-PEI-PEG-NH2 is an effective cargo for endostatin delivery with antiangiogenic effect in squamous cell carcinoma. © 2017 Samson A. Adeyemi et al.


Ngwuluka N.C.,Wits Advanced Drug Delivery Platform Research Unit | Choonara Y.E.,Wits Advanced Drug Delivery Platform Research Unit | Kumar P.,Wits Advanced Drug Delivery Platform Research Unit | Du Toit L.C.,Wits Advanced Drug Delivery Platform Research Unit | And 2 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2015

This study was undertaken to synthesize an interpo-lyelectrolyte complex (IPEC) of polymethacrylate (E100) and sodium carboxymethylcellulose (NaCMC) to form a polymeric hydrogel material for application in specialized oral drug delivery of sensitive levodopa. Computational modeling was employed to proffer insight into the interactions between the polymers. In addition, the reactional profile of NaCMC and polymethacrylate was elucidated using molecular mechanics energy relationships (MMER) and molecular dynamics simulations (MDS) by exploring the spatial disposition of NaCMC and E100 with respect to each other. Computational modeling revealed that the formation of the IPEC was due to strong ionic associations, hydrogen bonding, and hydrophilic interactions. The computational results corroborated well with the experimental and the analytical data. © 2014 Wiley Periodicals, Inc.


Ngwuluka N.C.,Wits Advanced Drug Delivery Platform Research Unit | Choonara Y.E.,Wits Advanced Drug Delivery Platform Research Unit | Kumar P.,Wits Advanced Drug Delivery Platform Research Unit | Du Toit L.C.,Wits Advanced Drug Delivery Platform Research Unit | And 2 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2015

This study was undertaken in order to apply a synthesized interpolyelectrolyte complex (IPEC) of polymethacry-late and carboxymethylcellulose as a controlled release oral tablet matrix for the delivery of the model neuroactive drug levodopa. The IPEC (synthesized in Part I of this work) was characterized by techniques such as Fourier Transform InfraRed (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), Advanced DSC (ADSC), and Scanning Electron Microscopy (SEM). The tablet matrices were formulated and characterized for their drug delivery properties and in vitro drug release. FTIR confirmed the interaction between the two polymers. The IPEC composite generated tablet matrices with a hardness ranging from 19.152-27.590 N/mm and a matrix resilience ranging between 42 and 46%. An IPEC of polyme-thacrylate and carboxymethylcellulose was indeed an improvement on the inherent properties of the native polymers providing a biomaterial with the ability to release poorly soluble drugs such as levodopa at a constant rate over a prolonged period of time. © 2014 Wiley Periodicals, Inc.

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