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Chang G.,Jiangnan University | Chang G.,Wilmar Biotechnology Research Development Center Ltd. | Gao N.,Wilmar Biotechnology Research Development Center Ltd. | Tian G.,Wilmar Biotechnology Research Development Center Ltd. | And 3 more authors.
Bioresource Technology | Year: 2013

Volumetric mass transfer coefficient (kLa) is a key fermentation parameter for the production of docosahexaenoic acid (DHA) from glycerol by Schizochytrium sp. S31. In order to elucidate the effects of kLa on the fermentations, both baffled and unbaffled flask cultures and fed-batch cultures were developed in present work. The results showed that high kLa could effectively increase the DHA concentration, DHA productivity and conversion yield (Yx/s, g/g). When kLa was set at 1802±105h-1 in the fed-batch culture, DHA concentration was achieved at 28.93g/L, DHA productivity at 301mg/L/h and Yx/s at 0.44±0.02g/g, all of which were significantly higher than those in the previous similar studies. © 2013 Elsevier Ltd. Source


Chang G.,Jiangnan University | Chang G.,Wilmar Biotechnology Research Development Center Ltd. | Wu J.,Wilmar Biotechnology Research Development Center Ltd. | Jiang C.,Wilmar Biotechnology Research Development Center Ltd. | And 4 more authors.
Bioresource Technology | Year: 2014

Three independent cultures by fed batch strategy under different oxygen supply levels were investigated with Schizochytrium sp. S31 on glycerol in 50L bioreactor. Three cultures all achieved high cell density cultivation (HCDC) with more than 100g/L biomass density. However, the culture with middle oxygen supply level achieved the highest DHA concentration at 21.26g/L. Dissolved oxygen (DO) limitation was commonly encountered in the present cultures, which was due to the dramatic decrease of kLa in high oxygen supply culture resulted from significantly increasing apparent viscosity of the broth. The rheological properties of the three cultures all exhibited shear-thinning behavior. The oxygen uptake rate (OUR) predominately influenced by kLa was suggested to replace DO as on-line control parameter for scale-up production of DHA. © 2013 Elsevier Ltd. Source

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