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Adhikari P.,Wilmar Biotechnology Research and Development Center Co. | Shin J.-A.,Chungnam National University | Lee J.-H.,Chungnam National University | Kim H.-R.,Kyungpook National University | And 3 more authors.
Food and Bioprocess Technology | Year: 2012

Interesterified hard fat (IEHF) was produced from fully hydrogenated soybean oil (FHSBO) and rice bran oil (RBO) with different molar ratio (RBO/FHSBO = 1:1, 1:2, and 1:3). For interesterification, Lipozyme TL IM (10% of total substrates) was used as a biocatalyst. Further, coconut oil (CO; 40 wt.% on total weight of RBO and FHSBO) was also added in all reactants for providing medium chain fatty acid. After interesterification, the obtained IEHF and physical blend (before interesterification) with same molar ratio were carried out for comparing the physical properties, (i. e., solid fat content, melting and crystallization behavior, and polymorphic forms). From DSC results at 25 °C, solid fat content of the IEHF with different molar ratio (RBO/FHSBO = 1:1, 1:2, and 1:3) were 33. 9%, 58. 8%, and 72. 1%, respectively, whereas physical blends at same molar ratio showed 66. 2%, 71. 6%, and 74. 8%. Besides, short spacing β crystal polymorphic form was observed in the physical blend while only β′ crystal form was observed in IEHF, in which β′ polymorphic form is a desirable for the production of shortenings and margarines. In Rancimat test for oxidative stability, IEHF showed significantly lower induction time than the physical blend. When the catechin (200, 400, and 800 ppm) was added to the IEHF, induction time was significantly increased to 21. 4, 34. 1, and 44. 3 h, respectively. In this study, IEHF from this study may have a potential functionality for the shortenings and margarines. © 2011 Springer Science + Business Media, LLC.

Zhang M.,Zhejiang University | Zhang M.,Wilmar Biotechnology Research and Development Center Co. | Zhang A.-T.,Zhejiang University | Zhang A.-T.,Feed Science Institute | And 6 more authors.
Indian Journal of Animal Sciences | Year: 2012

In order to understand the phylogenetic diversity and expression character of prolactin (PRL) molecule, the cDNA of PRL was isolated and cloned from female American silver king pigeon (Columbia livia) pituitary gland by a degenerate primer and nested reverse transcription polymerase chain reaction (RT-PCR) based strategy. The expected expression of mRNA in different tissues and status was quantified by real-time quantitative PCR (FQ-PCR). The cloned cDNA contains 658bp nucleotides, encoded 219 amino acids and deduced amino acid sequences showed high similarities and identity with the corresponding sequences of chicken, budgerigar, India pealfowl, comb duck, duck, goose, turkey, ring-necked pheasant, Java sparrow, ostrich, ring-necked pheasant and quail. PRL was widely expressed in tissues analyzed, and highest level of PRL mRNA expression was observed in the pitutary whereas the lowest in the kidney. Comparable high level of PRL mRNA expression was observed in oviduct and ovary. PRL mRNA expression increased significantly in incubation, compared to laying.

Chang G.,Jiangnan University | Chang G.,Wilmar Biotechnology Research and Development Center Co. | Luo Z.,Wilmar Biotechnology Research and Development Center Co. | Gu S.,Wilmar Biotechnology Research and Development Center Co. | And 3 more authors.
Bioresource Technology | Year: 2013

DHA production by Schizochytrium sp. S31 was studied in batch cultures on glycerol with stepwise dissolved oxygen strategy. Three growth stages were identified as cell growth, lipid accumulation and lipid turnover. It was revealed that fatty acid (FA) shifts during the three growth stages involved the activity changes of glycerol kinase (GK), FAD+-dependent glycerol-3-phosphate dehydrogenase (FAD+-G-3-PDH), malic enzyme (ME), ATP citrate lyase (ACL) and NAD+-dependent isocitrate dehydrogenase (NAD+-ICDH). Glycerol dissimilation in Schizochytrium sp. S31 was suggested via a phosphorylation by GK and a following oxidation by FAD+-G-3-PDH. Lipid accumulation of this strain was a growth-associated process, but the assimilable nitrogen depletion enhanced the accumulation of lipids. The exhaustion of glycerol induced the lipid turnover stage, where the short chain fatty acids were preferentially degraded and converted into lipid-free biomass (Xf) which was correlated to the increase of DHA content in biomass. © 2013 Elsevier Ltd.

Li J.,Jiangnan University | Liu R.,Jiangnan University | Chang G.,Wilmar Biotechnology Research and Development Center Co. | Li X.,Jiangnan University | And 4 more authors.
Bioresource Technology | Year: 2015

Glucose and glycerol are useful carbon sources for the cultivation of Aurantiochytrium limacinum SR21. Glucose facilitates rapid growth and lipid synthesis, and glycerol promotes the accumulation of docosahexaenoic acid (DHA) in A. limacinum SR21. To improve the DHA productivity of A. limacinum SR21, shake flask and fed-batch cultures were performed using glucose and glycerol as mixed carbon sources (MCSs). Along with optimization of the MCSs, the best DHA yield and productivity (32.36. g/L and 337.1. mg/L/h) were obtained via fed-batch fermentation with maintenance of a constant air supply. The DHA productivity was 15.24% higher than that obtained using glucose as single carbon source (SCS). This study presents a highly efficient and economic strategy for the production of DHA by A. limacinum SR21. © 2014 Elsevier Ltd.

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