Hildrun K.,Wildau University of Applied Sciences |
Alexander P.,RAS A.V. Zhirmunsky Institute of Marine Biology
Scientometrics | Year: 2012
Productivity and citedness of the staff of a German medical research institution are analyzed. It was found in our previous study (Pudovkin et al.: Scientometrics, doi:10. 1007/s11192-012-0659-z, 2012) that male scientists are more prolific and cited more often than female scientists. We explain in our present study one of the possible causes for obtaining this result with reference to Abramo et al. (Scientometrics 84(3): 821-833, 2009), who found in the small subgroups of star scientists a higher performance of male star scientists with respect to female star scientists; but in the remaining complementary subpopulations the performance gap between the two sexes is marginal. In agreement with Abramo et al. (2009), in our small subgroup of star scientists a higher performance of male star scientists with respect to female star scientists could be found. Contrasting, in the large complementary subgroup even a slightly higher performance of female scientists with respect to male scientists was identified. The last is even stronger expressed in favor of women than Abramo's result that the performance gap between the two sexes is truly marginal. In addition to Abramo et al. (2009), we already found in our previous study, special indexes characterizing the quality of papers (but not quantity) are not substantially different among sexes compared. © 2012 Akadémiai Kiadó, Budapest, Hungary.
Martens-Uzunova E.S.,Josephine Nefkens Institute |
Bottcher R.,Josephine Nefkens Institute |
Bottcher R.,Wildau University of Applied Sciences |
Croce C.M.,Ohio State University |
And 3 more authors.
European Urology | Year: 2014
Context Genomic regions without protein-coding potential give rise to millions of protein-noncoding RNA transcripts (noncoding RNA) that participate in virtually all cellular processes. Research over the last 10 yr has accumulated evidence that long noncoding RNAs (lncRNAs) are often altered in human urologic cancers. Objective To review current progress in the biology and implication of lncRNAs associated with prostate, bladder, and kidney cancer. Evidence acquisition The PubMed database was searched for articles in the English language with combinations of the Medical Subject Headings terms long non coding RNA, long noncoding RNA, long untranslated RNA, cancer, neoplasms, prostate, bladder, and kidney. Evidence synthesis We summarise existing knowledge on the systematics, biology, and function of lncRNAs, particularly these involved in prostate, kidney, and bladder cancer. We also discuss the possible utilisation of lncRNAs as novel biomarkers and potential therapeutic targets in urologic malignancies and portray the major challenges and future perspectives of ongoing lncRNA research. Conclusions LncRNAs are important regulators of gene expression interacting with the major pathways of cell growth, proliferation, differentiation, and survival. Alterations in the function of lncRNAs promote tumour formation, progression, and metastasis of prostate, bladder, and kidney cancer. LncRNAs can be used as noninvasive tumour markers in urologic malignancies. Increased knowledge of the molecular mechanisms by which lncRNAs perform their function in the normal and malignant cell will lead to a better understanding of tumour biology and could provide novel therapeutic targets for the treatment of urologic cancers. Patient summary In this paper we reviewed current knowledge of long noncoding RNAs (lncRNAs) for the detection and treatment of urologic cancers. We conclude that lncRNAs can be used as novel biomarkers in prostate, kidney, or bladder cancer. LncRNAs hold promise as future therapeutic targets, but more research is needed to gain a better understanding of their biologic function. © 2013 European Association of Urology.
Agency: Cordis | Branch: FP7 | Program: CP | Phase: ENERGY.2011.4.1-4 | Award Amount: 6.65M | Year: 2012
The COMTES project has as goal to develop and demonstrate three novel systems for compact seasonal storage of solar thermal energy. These systems will contribute to the EU 20-20-20 targets by covering a larger share of the domestic energy demand with solar thermal energy. Main objective of COMTES is to develop and demonstrate systems for seasonal storage that are significantly better than water based systems. The three technologies are covered in COMTES by three parallel development lines: solid sorption, liquid sorption and supercooling PCM. Strength of this approach is the collaboration of three development groups in activities that pertain to the analyses, methods and techniques that concern all technologies, without risking the exchange of confidential material. In this way, the development is much more effective than in three separate projects. The project starts with a definition of system boundary conditions and target applications. Next comes the investigation of the best available storage materials. Detailed numerical modelling of the physical processes, backed by experimental validations, will lead to optimum component design. Full-scale prototypes are simulated, constructed and tested in the laboratory in order to optimize process design. One year of fully monitored operation in demonstration buildings is followed by an integrated evaluation of the systems and their potential. When deemed successful, the involved industry partners will pick up the developed storage concepts and bring them further to a commercial level. The COMTES project is a cooperation of key scientific institutions active in the above mentioned heat storage technologies. For the first time, all relevant research disciplines are covered in an international effort. For each development line, a top-leading industry partner contributes its know-how and experience, providing the basis for further industrial development and exploitation of project results.
Tersch C.,Wildau University of Applied Sciences |
Lisdat F.,Wildau University of Applied Sciences
Electrochimica Acta | Year: 2011
In this work, the applicability of an impedimetric DNA sensor has been investigated for the detection of protein-DNA interactions. The sensor is based on short thiol-modified single-stranded DNA, which is chemisorbed to gold chip electrodes. In the presence of the redox system ferri-/ferrocyanide impedance measurements show an increase in charge transfer resistance after immobilization and hybridization of ssDNA to the sensor surface. The use of a longer capture oligonucleotide (a 25-mer instead of an 18-mer) results in a decreasing probe concentration on the surface. Furthermore it causes an increase of the charge transfer resistance for both ssDNA and dsDNA. The hybridization event, however, can be detected with a similar sensitivity compared to an 18-mer (with the same surface concentration) and allows a good discrimination between ssDNA and dsDNA. This electrode system is used to follow an enzyme reaction on the surface electrochemically. The cleavage of a double-stranded DNA by restriction endonuclease BamHI could be verified by cyclic voltammetry and impedance spectroscopy. The investigations are performed in dithiothreitol (DTT) free buffer solution since the incubation with DTT results in an alteration of the surface impedance. The sequence specific DNA-binding of the transcription factor NF-κB p50 is found to cause a decrease in charge transfer resistance. The signal change is concentration dependent and occurs due to a neutralization effect of the negatively charged DNA backbone. © 2011 Elsevier Ltd. All rights reserved.
Nietzold C.,Wildau University of Applied Sciences |
Lisdat F.,Wildau University of Applied Sciences
Analyst | Year: 2012
In this study we describe the use of gold nanoparticles as a fast detection system for the sensitive analysis of proteins. The immunological method allows for protein analysis at the nanogram level, as required for clinical diagnosis. Initially a test protein is used for the development of the assay. The system is subsequently adopted for alpha-fetoprotein, which is a relevant tumor marker. This work demonstrates that antibody functionalized gold nanoparticles can be used for the detection of proteins by forming gold nanoparticle aggregates. The influence of the size of the gold nanoparticles on the sensitivity of the assay is investigated in the range from 20-60 nm particles; the larger particles show here the highest relative changes. The formation of antigen-gold nanoparticle aggregates is detected by an increase in hydrodynamic diameter by dynamic light scattering (DLS). UV/Vis spectroscopy also allows assay monitoring by quantifying the red shift of the plasmon resonance wavelength. Alpha-fetoprotein can be analysed in the concentration range of 0.1-0.4 μg ml -1. The influence of pH, ionic strength and ratio of sample to Au-NP solution is studied. With this method, the protein AFP can be rapidly detected as demanded for clinical diagnosis. © 2012 The Royal Society of Chemistry.