Wildau University of Applied Sciences

www.th-wildau.de/
Wildau, Germany

The Technical University of Applied science Wildau is one of five universities of applied science in the federal state of Brandenburg, Germany. It is located near Germany's capital city, Berlin. Wikipedia.


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Janchen J.,Wildau University of Applied Sciences | Stach H.,ZeoSolar e.V.
Energy Procedia | Year: 2012

The water adsorption properties of modified porous sorbents for solar thermal energy storage and heat transformation have been investigated by thermogravimetry (TG) differential thermogravimetry (DTG), microcalorimetry, measurements of water adsorption isotherms, and storage tests. A chabazite type SAPO, a dealuminated faujasite type zeolite, and a mesostructured aluminosilicate, have been synthesized and compared with common zeolites X, Y and silica gel. It has been found that optimized lattice composition and pore architecture contribute to well adapt hydrophilic properties and a beneficial steep isotherm. © 2012 The Authors.


Tersch C.,Wildau University of Applied Sciences | Lisdat F.,Wildau University of Applied Sciences
Electrochimica Acta | Year: 2011

In this work, the applicability of an impedimetric DNA sensor has been investigated for the detection of protein-DNA interactions. The sensor is based on short thiol-modified single-stranded DNA, which is chemisorbed to gold chip electrodes. In the presence of the redox system ferri-/ferrocyanide impedance measurements show an increase in charge transfer resistance after immobilization and hybridization of ssDNA to the sensor surface. The use of a longer capture oligonucleotide (a 25-mer instead of an 18-mer) results in a decreasing probe concentration on the surface. Furthermore it causes an increase of the charge transfer resistance for both ssDNA and dsDNA. The hybridization event, however, can be detected with a similar sensitivity compared to an 18-mer (with the same surface concentration) and allows a good discrimination between ssDNA and dsDNA. This electrode system is used to follow an enzyme reaction on the surface electrochemically. The cleavage of a double-stranded DNA by restriction endonuclease BamHI could be verified by cyclic voltammetry and impedance spectroscopy. The investigations are performed in dithiothreitol (DTT) free buffer solution since the incubation with DTT results in an alteration of the surface impedance. The sequence specific DNA-binding of the transcription factor NF-κB p50 is found to cause a decrease in charge transfer resistance. The signal change is concentration dependent and occurs due to a neutralization effect of the negatively charged DNA backbone. © 2011 Elsevier Ltd. All rights reserved.


Nietzold C.,Wildau University of Applied Sciences | Lisdat F.,Wildau University of Applied Sciences
Analyst | Year: 2012

In this study we describe the use of gold nanoparticles as a fast detection system for the sensitive analysis of proteins. The immunological method allows for protein analysis at the nanogram level, as required for clinical diagnosis. Initially a test protein is used for the development of the assay. The system is subsequently adopted for alpha-fetoprotein, which is a relevant tumor marker. This work demonstrates that antibody functionalized gold nanoparticles can be used for the detection of proteins by forming gold nanoparticle aggregates. The influence of the size of the gold nanoparticles on the sensitivity of the assay is investigated in the range from 20-60 nm particles; the larger particles show here the highest relative changes. The formation of antigen-gold nanoparticle aggregates is detected by an increase in hydrodynamic diameter by dynamic light scattering (DLS). UV/Vis spectroscopy also allows assay monitoring by quantifying the red shift of the plasmon resonance wavelength. Alpha-fetoprotein can be analysed in the concentration range of 0.1-0.4 μg ml -1. The influence of pH, ionic strength and ratio of sample to Au-NP solution is studied. With this method, the protein AFP can be rapidly detected as demanded for clinical diagnosis. © 2012 The Royal Society of Chemistry.


Tanne C.,Wildau University of Applied Sciences | Gobel G.,Wildau University of Applied Sciences | Lisdat F.,Wildau University of Applied Sciences
Biosensors and Bioelectronics | Year: 2010

In this study a biofuel cell anode is developed on the basis of multi-walled carbon nanotubes (MWCNTs). Recombinant pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase (GDH) is covalently coupled to a PQQ-layer which is adsorbed onto thiol-modified MWCNTs at a gold electrode. In the presence of glucose a catalytic current starts at a potential of -80mV vs. Ag/AgCl, 1M KCl. Under substrate saturation current densities of 170-200μA/cm2 can be achieved. The operation is based on mediated electron transfer of the enzyme.This (PQQ)-GDH-MWCNT-electrode is combined with a MWCNT-modified electrode to which bilirubin oxidase (BOD) is covalently coupled. The resulting membrane-free biofuel cell has an open cell potential of 600mV and can achieve a power density in the range of 23μW/cm2. © 2010 Elsevier B.V.


Schubart I.W.,Wildau University of Applied Sciences | Gobel G.,Wildau University of Applied Sciences | Lisdat F.,Wildau University of Applied Sciences
Electrochimica Acta | Year: 2012

In this study we present a pyrroloquinolinequinone-dependent glucose dehydrogenase [(PQQ)-GDH] electrode with direct electron transfer between the enzyme and electrode. Soluble pyrroloquinolinequinone-dependent glucose dehydrogenase from Acinetobacter calcoaceticus is covalently bound to an electropolymerized polyaniline copolymer film on a multi-walled carbon nanotube (MWCNT)-modified gold electrode. The pulsed electropolymerization of 2-methoxyaniline-5-sulfonic acid (MASA) and m-aminobenzoic acid (ABA) is optimized with respect to the efficiency of the bioelectrocatalytic conversion of glucose. The glucose oxidation starts at -0.1 V vs. Ag/AgCl and current densities up to 500 μA/cm 2 at low potential of +0.1 V vs. Ag/AgCl can be achieved. The electrode shows a glucose sensitivity in the range from 0.1 mM to 5 mM at a potential of +0.1 V vs. Ag/Ag/Cl. The dynamic range is extended to 100 mM at +0.4 V vs. Ag/AgCl. The electron transfer mechanism is studied and buffer effects are investigated. The developed enzyme electrode is examined for bioenergetic application by assembling of a membrane-less biofuel cell. For the cathode a bilirubin oxidase (BOD) based MWCNT-modified gold electrode with direct electron transfer (DET) is used. The biofuel cell exhibits a cell potential of 680 ± 20 mV and a maximum power density of up to 65 μW/cm 2 at 350 mV vs. Ag/AgCl.


Goldenberg L.M.,Wildau University of Applied Sciences | Lisinetskii V.,Wildau University of Applied Sciences | Gritsai Y.,Institute of Thin Film Technology and Microsensorics | Stumpe J.,Fraunhofer Institute for Applied Polymer Research | Schrader S.,Wildau University of Applied Sciences
Advanced Materials | Year: 2012

Distributed feedback (DFB) lasers are produced directly in fluorescent azobenzene-containing materials using a single holographic optical step. Surface relief grating capable of producing images in fluorescence microscopy can be holographically formed in a number of materials. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Hildrun K.,Wildau University of Applied Sciences | Alexander P.,RAS A.V. Zhirmunsky Institute of Marine Biology
Scientometrics | Year: 2012

Productivity and citedness of the staff of a German medical research institution are analyzed. It was found in our previous study (Pudovkin et al.: Scientometrics, doi:10. 1007/s11192-012-0659-z, 2012) that male scientists are more prolific and cited more often than female scientists. We explain in our present study one of the possible causes for obtaining this result with reference to Abramo et al. (Scientometrics 84(3): 821-833, 2009), who found in the small subgroups of star scientists a higher performance of male star scientists with respect to female star scientists; but in the remaining complementary subpopulations the performance gap between the two sexes is marginal. In agreement with Abramo et al. (2009), in our small subgroup of star scientists a higher performance of male star scientists with respect to female star scientists could be found. Contrasting, in the large complementary subgroup even a slightly higher performance of female scientists with respect to male scientists was identified. The last is even stronger expressed in favor of women than Abramo's result that the performance gap between the two sexes is truly marginal. In addition to Abramo et al. (2009), we already found in our previous study, special indexes characterizing the quality of papers (but not quantity) are not substantially different among sexes compared. © 2012 Akadémiai Kiadó, Budapest, Hungary.


Grant
Agency: European Commission | Branch: FP7 | Program: CP | Phase: ENERGY.2011.4.1-4 | Award Amount: 6.65M | Year: 2012

The COMTES project has as goal to develop and demonstrate three novel systems for compact seasonal storage of solar thermal energy. These systems will contribute to the EU 20-20-20 targets by covering a larger share of the domestic energy demand with solar thermal energy. Main objective of COMTES is to develop and demonstrate systems for seasonal storage that are significantly better than water based systems. The three technologies are covered in COMTES by three parallel development lines: solid sorption, liquid sorption and supercooling PCM. Strength of this approach is the collaboration of three development groups in activities that pertain to the analyses, methods and techniques that concern all technologies, without risking the exchange of confidential material. In this way, the development is much more effective than in three separate projects. The project starts with a definition of system boundary conditions and target applications. Next comes the investigation of the best available storage materials. Detailed numerical modelling of the physical processes, backed by experimental validations, will lead to optimum component design. Full-scale prototypes are simulated, constructed and tested in the laboratory in order to optimize process design. One year of fully monitored operation in demonstration buildings is followed by an integrated evaluation of the systems and their potential. When deemed successful, the involved industry partners will pick up the developed storage concepts and bring them further to a commercial level. The COMTES project is a cooperation of key scientific institutions active in the above mentioned heat storage technologies. For the first time, all relevant research disciplines are covered in an international effort. For each development line, a top-leading industry partner contributes its know-how and experience, providing the basis for further industrial development and exploitation of project results.


Martens-Uzunova E.S.,Erasmus University Rotterdam | Bottcher R.,Erasmus University Rotterdam | Bottcher R.,Wildau University of Applied Sciences | Croce C.M.,Ohio State University | And 3 more authors.
European Urology | Year: 2014

Context Genomic regions without protein-coding potential give rise to millions of protein-noncoding RNA transcripts (noncoding RNA) that participate in virtually all cellular processes. Research over the last 10 yr has accumulated evidence that long noncoding RNAs (lncRNAs) are often altered in human urologic cancers. Objective To review current progress in the biology and implication of lncRNAs associated with prostate, bladder, and kidney cancer. Evidence acquisition The PubMed database was searched for articles in the English language with combinations of the Medical Subject Headings terms long non coding RNA, long noncoding RNA, long untranslated RNA, cancer, neoplasms, prostate, bladder, and kidney. Evidence synthesis We summarise existing knowledge on the systematics, biology, and function of lncRNAs, particularly these involved in prostate, kidney, and bladder cancer. We also discuss the possible utilisation of lncRNAs as novel biomarkers and potential therapeutic targets in urologic malignancies and portray the major challenges and future perspectives of ongoing lncRNA research. Conclusions LncRNAs are important regulators of gene expression interacting with the major pathways of cell growth, proliferation, differentiation, and survival. Alterations in the function of lncRNAs promote tumour formation, progression, and metastasis of prostate, bladder, and kidney cancer. LncRNAs can be used as noninvasive tumour markers in urologic malignancies. Increased knowledge of the molecular mechanisms by which lncRNAs perform their function in the normal and malignant cell will lead to a better understanding of tumour biology and could provide novel therapeutic targets for the treatment of urologic cancers. Patient summary In this paper we reviewed current knowledge of long noncoding RNAs (lncRNAs) for the detection and treatment of urologic cancers. We conclude that lncRNAs can be used as novel biomarkers in prostate, kidney, or bladder cancer. LncRNAs hold promise as future therapeutic targets, but more research is needed to gain a better understanding of their biologic function. © 2013 European Association of Urology.


Grant
Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: SIS-2010-1.3.3.1 | Award Amount: 2.03M | Year: 2011

Assessment of the performance of individual researchers is the cornerstone of the scientific and scholarly workforce. It shapes the quality and relevance of knowledge production in science, technology and innovation. Currently, there is a discrepancy between the criteria used in performance assessment and the broader social and economic function of scientific and scholarly research. Additional problems in the current evaluation system are: lack of resources for qualitative evaluation due to increased scale of research; available quantitative measures are often not applicable at the individual level; and there is a lack of recognition for new types of work that researchers need to perform. As a result, the broader social functions of the scientific system are often not included in its quality control mechanisms. Academic Careers Understood through Measurement and Norms (ACUMEN) addresses these problems by developing criteria and guidelines for Good Evaluation Practices (GEP). These will be based on an ACUMEN Portfolio for individual researchers throughout the sciences, social sciences and humanities combining multiple qualitative and quantitative evidence sources. The ACUMEN Portfolio will be based on: a comparative analysis of current peer review systems in Europe; an in-depth exploration of novel peer review practices; an assessment of the use of scientometric indicators in performance evaluation; the development of new web-based indicators and web-based evaluation tools; and a comparative analysis of the implications of current and emerging evaluation practices for the career development of women researchers. ACUMEN is an integrated, comparative study in which a set of proven methods will be used on the basis of selections from one shared data set: a sample of European Research Area personnel from bibliographic and web databases as well as data harvested from websites, and data gathered through interviews and from citation indexes.

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