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Nan K.,Whenzhou Medical College | Nan K.,Institute of Biomedical Engineering | Nan K.,Southern Medical University | Sun S.,Whenzhou Medical College | And 6 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2010

Bone substitute materials can induce bone formation when combined with mesenchymal stem cells (MSC). The aim of the current study was to examine in vivo ectopic bone formation with MSC on tricalcium phosphate (TCP) ceramics. Osteoblasts isolated from bone marrow stromal cells (BMSCs) of New Zealand rabbits were cultured with TCP ceramics for 10 days, followed by implantation of the cultured TCP ceramics into the rabbit dorsum muscle. The cultured TCP and in vivo new bone formation with TCP biodegradation were evaluated histologically. Scanning electron microscopy showed that the surface of the cultured TCP ceramics was filled by osteoblasts with a cell-free zone in the central area. New bone was formed on the cultured TCP ceramics with signs of gradual degradation of TCP ceramics at 8 weeks of implantation, indicating that TCP could be a potential scaffold for seeding cells used for development of bioengineering tissues. © 2009 Wiley Periodicals, Inc.


Liu J.,Whenzhou Medical College | Xu L.,Whenzhou Medical College | Liu C.,Whenzhou Medical College | Zhang D.,Whenzhou Medical College | And 6 more authors.
Carbohydrate Polymers | Year: 2012

In the present paper, cationic nanoparticles of curcumin, chitosan and poly(ε-caprolactone) were developed by a simple nano-precipitation method. The developed curcumin loaded chitosan/poly(ε-caprolactone) (chitosan/PCL) nanoparticle showed almost spherical shape and its diameter was varied between 220 nm and 360 nm and zeta potential was varied between +30 mV and 0 mV as a function with pH value. The encapsulation of curcumin into nanoparticles was confirmed by fluorescence spectral analysis. In vitro release study showed the sustained release behavior of curcumin from nanoparticles during the period of 5 days study. In vitro cytotoxicity test revealed the drug concentration dependent on the cell viability against Hela cells and OCM-1 cells after 48 h co-incubation. Furthermore, in vitro cell uptake study revealed that the cell uptake of curcumin was greatly enhanced by encapsulated curcumin into cationic chitosan/PCL nanoparticles. Therefore, the developed cationic chitosan/PCL nanoparticles might be a promising candidate for curcumin delivery to cancer cells. © 2012 Elsevier Ltd.

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