Entity

Time filter

Source Type

Taoyuan City, Taiwan

Lu Z.,Zhejiang Ocean University | Liu W.,Zhejiang Ocean University | Liu L.,Zhejiang Ocean University | Shi H.,Marine Fisheries Research Institute of Zhejiang | And 7 more authors.
Genomics Data | Year: 2016

The common Chinese cuttlefish (Sepiella japonica) has been considered one of the most economically important marine Cephalopod species in East Asia and seed breeding technology has been established for massive aquaculture and stock enhancement. In the present study, we used Illumina HiSeq2000 to sequence, assemble and annotate the transcriptome of the ovary tissues of S. japonica for the first time. A total of 53,116,650 and 53,446,640 reads were obtained from the immature and matured ovaries, respectively (NCBI SRA database SRX1409472 and SRX1409473), and 70,039 contigs (N50 = 1443 bp) were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 47,288 contigs show differential expression profile and 793 contigs are highly expressed in the immature ovary, while 38 contigs are highly expressed in the mature ovary with FPKM > 100. We hope that the ovarian transcriptome and those stage-enriched transcripts of S. japonica can provide some insight into the understanding of genome-wide transcriptome profile of cuttlefish gonad tissue and give useful information in cuttlefish gonad development. © 2015 The Authors. Source


Shen K.-N.,National Taiwan Ocean University | Chang C.-W.,National Museum of Marine Biology and Aquarium | Chang C.-W.,National Dong Hwa University | Chan Y.-F.,Chung Yuan Christian University | And 4 more authors.
Mitochondrial DNA | Year: 2015

In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of Woodhead's angelfish (Centropyge woodheadi) and Herald's angelfish (Centropyge heraldi) for the first time. The assembled mitogenome, consisting of 16 863 and 16 836 bp, shows 95.3% identity to each other. Both mitogenome follow a typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop contains 1158 bp in C. woodheadi and 1129 bp in C. heraldi and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 47.9% for C. woodheadi and 47.5% for C. heraldi. The complete mitogenomes of C. woodheadi and C. heraldi provide an essential and important DNA molecular data for phylogenetic and evolutionary analysis for marine angelfish. © 2015 Taylor & Francis. Source


Shen K.-N.,National Taiwan Ocean University | Chang C.-W.,National Museum of Marine Biology and Aquarium | Chang C.-W.,National Dong Hwa University | Lin Z.-H.,Chung Yuan Christian University | And 4 more authors.
Mitochondrial DNA | Year: 2015

In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of Cocos lemonpeel angelfish (Centropyge flavissima) and Eibl’s angelfish (Centropyge eibli) for the first time. The assembled mitogenome, consisting of 17 010 bp and 17 101 bp, showing 98.3% identity each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein coding genes, 22 transfer RNAs, two ribosomal RNA genes, and a non-coding control region of D-loop. D-loop contains 1302 bp in C. flavissima and 1392 bp in C. eibli and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 45.2% for C. flavissima and 44.9% for C. eibli. Complete mitogenomes of C. flavissima and C. eibli provide essential and important DNA molecular data for phylogenetic and evolutionary analysis for marine angelfishes. © 2015 Taylor & Francis. Source


Shen K.-N.,National Taiwan Ocean University | Chang C.-W.,National Museum of Marine Biology and Aquarium | Chang C.-W.,National Dong Hwa University | Chen C.-H.,WeThink Biotech Inc | Hsiao C.-D.,Chung Yuan Christian University
Mitochondrial DNA | Year: 2015

In this study, we used the next-generation sequencing method to deduce complete mitogenomes of Armitage angelfish (Apolemichthys armitagei) and Griffisi angelfish (A. griffisi) for the first time. The assembled mitogenomes, consists 16,551 bp (A. armitagei) and 16,528 bp (A. griffisi) in length, shows 92% identity each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein coding genes, 22 transfer RNAs, two ribosomal RNAs genes, and a non-coding control region of D-loop. D-loop contains a length of 828 bp in A. armitagei and 849 bp in A. griffisi and locates between tRNA-Pro and tRNA-Phe. Complete mitogenomes of Armitage angelfish and Griffisi angelfish deduced in this study provide essential and important DNA molecular data for further phylogenetic and evolutionary analysis for marine angelfish. © 2015 Taylor & Francis. Source


Xie Q.,Xinjiang Normal University | Shen K.-N.,National Taiwan Ocean University | Hao X.,Xinjiang Academy of Agricutural Science | Nam P.N.,Ton Duc Thang University | And 7 more authors.
Mitochondrial DNA | Year: 2015

We decoded the complete chloroplast DNA (cpDNA) sequence of the Tianshan Snow Lotus (Saussurea involucrata), a famous traditional Chinese medicinal plant of the family Asteraceae, by using next-generation sequencing technology. The genome consists of 152 490 bp containing a pair of inverted repeats (IRs) of 25 202 bp, which was separated by a large single-copy region and a small single-copy region of 83 446 bp and 18 639 bp, respectively. The genic regions account for 57.7% of whole cpDNA, and the GC content of the cpDNA was 37.7%. The S. involucrata cpDNA encodes 114 unigenes (82 protein-coding genes, 4 rRNA genes, and 28 tRNA genes). There are eight protein-coding genes (atpF, ndhA, ndhB, rpl2, rpoC1, rps16, clpP, and ycf3) and five tRNA genes (trnA-UGC, trnI-GAU, trnK-UUU, trnL-UAA, and trnV-UAC) containing introns. A phylogenetic analysis of the 11 complete cpDNA from Asteracease showed that S. involucrata is closely related to Centaurea diffusa (Diffuse Knapweed). The complete cpDNA of S. involucrata provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for Asteraceae. © 2015 Taylor & Francis Source

Discover hidden collaborations