West Virginia State University is a historically black public university in Institute, West Virginia, United States. In the Charleston-metro area, the school is usually referred to simply as "State" or "West Virginia State". It is one of the original 1890 Land-Grant colleges and the smallest land-grant institution in the country. The University is a member-school of Thurgood Marshall College Fund. Wikipedia.
Jagadeeswaran G.,Oklahoma State University |
Nimmakayala P.,West Virginia State University |
Zheng Y.,Fudan University |
Gowdu K.,Oklahoma State University |
And 2 more authors.
BMC Genomics | Year: 2012
Background: MicroRNAs (miRNAs) are a class of non-coding small RNAs involved in post-transcriptional regulation of gene expression critical for plant growth and development, stress responses and other diverse biological processes in plants. The Cucurbitaceae or cucurbit family represents some of economically important species, particularly those with edible and medicinal fruits. Genomic tools for the molecular analysis of members of this family are just emerging. Partial draft genome sequence became available recently for cucumber and watermelon facilitating investigation of the small RNA component of the transcriptomes in cucurbits.Results: We generated four small RNA libraries from bottle gourd (Lagenaria siceraria), Cucurbita moschata, Cucurbita pepo, and, watermelon (Citrullus lanatus var. lanatus) in order to identify conserved and novel lineage specific miRNAs in these cucurbits. Deep sequencing of small RNA libraries from these species resulted in 1,597,263, 532,948, 601,388, and 493,384 unique sRNA reads from bottle gourd, moschata, pepo and watermelon, respectively. Sequence analysis of these four libraries resulted in identification of 21 miRNA families that are highly conserved and 8 miRNA families that are moderately conserved in diverse dicots. We also identified 4 putative novel miRNAs in these plant species. Furthermore, the tasiRNAs were identified and their biogenesis was determined in these cucurbits. Small RNA blot analysis or q-PCR analyses of leaf and fruit tissues of these cucurbits showed differential expression of several conserved miRNAs. Interestingly, the abundance of several miRNAs in leaves and fruits of closely related C. moschata and C. pepo was also distinctly different. Target genes for the most conserved miRNAs are also predicted.Conclusion: High-throughput sequencing of small RNA libraries from four cucurbit species has provided a glimpse of small RNA component in their transcriptomes. The analysis also showed considerable variation within four cucurbit species with regards to expression of individual miRNAs. © 2012 Jagadeeswaran et al.; licensee BioMed Central Ltd.
Hass A.,U.S. Department of Agriculture |
Hass A.,West Virginia State University |
Fine P.,Institute of Soil, Water and Environmental Sciences
Critical Reviews in Environmental Science and Technology | Year: 2010
The authors review selected protocols of sequential selective extraction procedure that are used to characterize the geochemical distribution of heavy metals in soils, wastes, and sediments. They discuss the development of earlier protocols, their modifications, and the extent to which a given protocol pertains to different conditions. Emphasis is given to the considerations that led to a choice of reagents for each step and to their order in the sequence. Published studies are used as case studies to critically evaluate the implied geochemical components of operationally defined extraction steps. Also assessed are possible effects of subsequent extraction steps and conditions on the selective dissolution of the solid components and their operational definitions. Copyright © 2010 Taylor & Francis Group, LLC.
Yossa R.,West Virginia State University
Journal of Applied Aquaculture | Year: 2014
Good presentation of a manuscript is essential for its fast and successful editorial review. A well-written and thoroughly organized manuscript is easy to read and presents data in a manner easy to understand without a lot of repetition. Editors and reviewers are more inclined to engage with authors to improve scientific quality of well-presented manuscripts, further increasing the chances of publication. Although general approaches to scientific writing have been published, little attention has been focused on scientific writing in aquaculture research. Here, we provide students and less-experienced aquaculture researchers with some insights on research organization and manuscript presentation in order to improve their chances of getting their research into print. © , Copyright © Taylor & Francis Group, LLC.
Schedl A.,West Virginia State University
Journal of Geology | Year: 2015
Distal ejecta from meteorite impacts are rare and hard to spot in the field because of their thinness (<1 m). This article suggests looking for distal ejecta in association with larger sedimentary structures, seismites, slope failure features (slumps, turbidites, and submarine landslides), and offshore tsunamites (water depths ≥25 m) in former epeiric seas. Distal ejecta tend to overlie seismites and slope failure features because seismic waves travel faster than ejecta. In epeiric seas, impact-generated tsunamis travel slower (25–85 m/s) than ejecta, so the tsunamis rework ejecta deposits. However, if a tsunami moves ‘from shallow to deeper water, then it buries the ejecta deposit, preserving it, as observed for Acraman. Impact-generated offshore-tsunami deposits (≥25 m water depths) are potentially distinct from those generated by cratonic earthquakes. The impact tsunamis can transport >5-mm-diameter sediments and produce high-flow-regime bedforms, megaripples, dunes, upper-plane beds, and possibly antidunes, whereas earthquake tsunamis cannot produce these features in an offshore environment. The last section of the article discusses the relationship of the physical character of the ejecta (thickness, accretionary lapilli diameter, and maximum grain size of shocked quartz) and distance to and size of impact. This information could be used (1) to link a particular ejecta layer to a particular crater, (2) to put constraints on where to look for a crater associated with a particular ejecta layer (Alamo, 250–325 km from the center of the crater; Stac Fada, 225–300 km from the center of the crater), and (3) to provide constraints on the size of a crater, which may have been destroyed by erosion and tectonics (Alamo, 40–70 km diameter; Stac Fada, 80–150 km). © 2015 by The University of Chicago.
Barney R.J.,West Virginia State University
Coleopterists Bulletin | Year: 2016
Eight species of Pachybrachis Chevrolat have been found to be endemic to Florida: Pachybrachis archboldi Barney, new species; Pachybrachis clarki Barney, new species; Pachybrachis conformis Suffrian; Pachybrachis deyrupi Barney, new species; Pachybrachis discoideus Bowditch; Pachybrachis illectus Fall; Pachybrachis osceola Fall; and Pachybrachis rileyi Barney, new species. Types were examined for the previously named species and aedeagi illustrated for the first time. A description, figure, and range map is presented for each species.