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Hwang U.-K.,West Sea Fisheries Research Institute WSFRI | Heo S.,West Sea Fisheries Research Institute WSFRI | Choi O.-I.,West Sea Fisheries Research Institute WSFRI | Park J.-S.,West Sea Fisheries Research Institute WSFRI | Lee K.-S.,Mokpo Maritime University
Toxicology and Environmental Health Sciences | Year: 2010

Effect of aluminum (al) on the plasma vitellogenin (VTG), alkaline-labile phosphorus (ALPP), Calcium (Ca), glutamate pyruvate transaminase (GPT) and hepatosomatic index (HSI) were examined in the estradiol-17β (E2)-administered immature rockfish, Sebastes schlegeli. Fish were intraperitoneally injected with E2 (5 mg/kg body weight (B. W.)) and/or Al (0, 0. 1, 1, 5 and 10 mg/kg B. W.) and then plasma were extracted at 7 days. Thick VTG band was detected at a molecular weight position of about 170 kDa in SDSPAGE using the plasma of E2-administered rockfish. This band was reduced by the administration of Al to rockfish. Al reduced the concentrations of ALPP and Ca in a concentration-dependent way and a significant reduction occurred at Al concentrations greater than 5 mg/kg and 10 mg/kg, respectively. The concentration of GPT was increased in concentrationdependent way at all the group of Al-administered rockfish. HSI was reduced in a concentration-dependent way. These results suggest that Al interfere with E2-induced vitellogenesis by being toxic to hepatocyte. © 2010 The Korean Society of Environmental Risk Assessment and Health Science and Springer. Source


Hwang U.-K.,West Sea Fisheries Research Institute WSFRI | Kang H.-S.,West Sea Fisheries Research Institute WSFRI | Lee Y.,West Sea Fisheries Research Institute WSFRI | Shon J.-K.,West Sea Fisheries Research Institute WSFRI
Fisheries and Aquatic Science | Year: 2012

Effects of aluminum (Al) on plasma vitellogenin (VTG), alkaline-labile phosphorus (ALPP), calcium (Ca), glutamate pyruvate transaminase (GPT), the hepatosomatic index (HSI), and hepatic Al concentration were examined in estradiol-17β (E2)-administered immature rockfish Sebastes schlegeli. Fish were injected intraperitoneally with E2 (5 mg/kg body weight [BW]) and/or Al (0, 0.1, 1, 5, and 10 mg/kg BW) and plasma and liver samples were extracted 7 days later. After sodium dodecyl sulfate polyacrylamide gel electrophoresis, the relative amount of VTG was determined by integrated optical density. VTG accounted for 23.6% of the total proteins in the control group, but this value decreased with increasing Al administration. Al reduced the concentrations of ALPP and Ca in a concentration-dependent manner and significant reduction occurred at Al concentrations greater than 5 mg/kg. The concentration of GPT increased in a concentration-dependent manner in all Al-administered rockfish. The concentrations of Al in the liver also increased, and HSI was decreased, in a concentration-dependent manner. These results suggest that Al inhibits E2-induced VTG production by being toxic to hepatocytes in marine fish. © The Korean Society of Fisheries and Aquatic Science. Source

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