West China Hospital of Stomatology

Chengdu, China

West China Hospital of Stomatology

Chengdu, China
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PubMed | Sun Yat Sen University, West China Hospital of Stomatology, Guangzhou University, Zhengzhou University and 3 more.
Type: Journal Article | Journal: Journal of cellular and molecular medicine | Year: 2016

Periodontitis is a chronic inflammatory disease which is caused by bacterial infection and leads to the destruction of periodontal tissues and resorption of alveolar bone. Thus, special attention should be paid to the mechanism under lipopolysaccharide (LPS)-induced periodontitis because LPS is the major cause of periodontitis. However, to date, miRNA expression in the LPS-induced periodontitis has not been well characterized. In this study, we investigated miRNA expression patterns in LPS-treated periodontal ligament cells (PDLCs). Through miRNA array and differential analysis, 22 up-regulated miRNAs and 28 down-regulated miRNAs in LPS-treated PDLCs were identified. Seven randomly selected up-regulated (miR-21-5p, 498, 548a-5p) and down-regulated (miR-495-3p, 539-5p, 34c-3p and 7a-2-3p) miRNAs were examined by qRT-PCR, and the results proved the accuracy of the miRNA array. Moreover, targets of these deregulated miRNAs were analysed using the miRWalk database. Database for Annotation, Visualization and Integration Discovery software were performed to analyse the Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes pathway of differential expression miRNAs, and the results shown that Toll-like receptor signalling pathway, cAMP signalling pathway, transforming growth factor-beta signalling pathway, mitogen-activated protein kinase (MAPK) signalling pathway and other pathways were involved in the molecular mechanisms underlying LPS-induced periodontitis. In conclusion, this study provides clues for enhancing our understanding of the mechanisms and roles of miRNAs as key regulators of LPS-induced periodontitis.


Man Y.,West China Hospital of Stomatology | Wang T.,West China Hospital of Stomatology | Mo A.,West China Hospital of Stomatology | Qu Y.,West China Hospital of Stomatology
Journal of Prosthetic Dentistry | Year: 2014

An implant was malpositioned and osseointegrated at the maxillary sinus floor with no complications. However, unexpected bone formation over the implant made the implant nonfunctional. Because the patient rejected the removal of the implant, it was left in place while another short implant was placed into the newly formed bone in approximately the same position as the old implant to restore the posterior maxillary tooth. Within 6 months, the new implant over the old asymptomatic implant was functional. Radiographs revealed osseointegration and stable periimplant marginal bone level with no signs of infection or inflammation. © 2014 Editorial Council for the Journal of Prosthetic Dentistry.


Wang K.,West China Hospital of Stomatology | Miao T.,University of Sichuan | Lu W.,West China Hospital of Stomatology | He J.,West China Hospital of Stomatology | And 4 more authors.
Microbiology and Immunology | Year: 2015

Oral lichen planus (OLP) is a chronic inflammatory disorder of oral mucosa of unknown cause. Microbial infection and dysimmunity appear to play important roles in its pathogenesis. In this study, differences in genetic profiling of salivary microbial communities in two subtypes of OLP and healthy controls were evaluated by means of PCR-denaturing gradient gel electrophoresis (DGGE). Additionally, ELISA was used to investigate the possible role of Th17 in lesion formation by detecting two related cytokines IL-17 and IL-23 in the saliva of OLP patients. When the DGGE profiles were analyzed, the bacterial populations were found to be significantly less rich in subjects with reticular and erosive OLP than in healthy controls. There was significantly less microbial diversity, as denoted by the Shannon index, in saliva samples from subjects with erosive OLP than in those from healthy controls. Cluster analysis and principal component analysis showed that the DGGE profiles formed distinctly group-specific clusters. Salivary concentrations of IL-17 in subjects with erosive OLP group were significantly higher than in those with reticular OLP and healthy controls. What's more, significantly positive correlations were observed between salivary IL-17 concentrations and disease clinical scores. Microbial richness and diversity was negatively correlated with salivary IL-17 concentrations. These results suggest there is significantly less salivary bacterial diversity and complexity in subjects with OLP han in healthy controls and that the shifted community composition is closely related to an immune cytokine, IL-17. © 2015 The Societies and Wiley Publishing Asia Pty Ltd.


Peng L.,West China Hospital of Stomatology | Peng L.,University of Sichuan | Ren L.B.,West China Hospital of Stomatology | Dong G.,West China Hospital of Stomatology | And 5 more authors.
International Endodontic Journal | Year: 2010

Aim: To investigate the role of Wnt5a in the process of differentiation of human dental papilla cells (HDPCs). Methodology: Recombinant adenovirus encoding full-length Wnt5a cDNA was constructed to investigate the biological role of Wnt5a on the differentiation of HDPCs. The effect of Wnt5a on HDPCs differentiation was determined by ALP activity assay, ALP staining and mineral induction assay. Mineralization-related gene expressions were assessed by RT-PCR. Results: Immunostaining revealed Wnt5a expression in the odontoblast layer and dental papilla tissue. Over-expression of Wnt5a by transfecting HDPCs with an Wnt5a-carrying construct increased ALPase activity and the formation of mineralized nodules of HDPCs. RT-PCR analysis showed that the expressions of mineralization-related genes, such as bone sialoprotein, collagen type I, osteonectin, osteopontin (OCN), dentine matrix protein-1 were up-regulated by Wnt5a. Conclusions: Wnt5a promoted differentiation of HDPCs. © 2010 International Endodontic Journal.


Li Y.,West China Hospital of Stomatology | He D.,West China Hospital of Stomatology | Liu B.,West China Hospital of Stomatology | Hu J.,West China Hospital of Stomatology
Journal of Biomedical Materials Research - Part B Applied Biomaterials | Year: 2016

The aim of this study was to evaluate the effect of SEMA3A released from matrigel on implant fixation in ovariectomized (OVX) rats. Sixty female rats were subjected to bilateral ovariectomy. Twelve weeks later, rats were randomly divided into three groups according to implants they accepted: (1) Control, implants with distilled water; (2) Matrigel, implants with matrigel coating; (3) Matrigel+SEMA3A, implants with coating of SEMA3A suspended in matrigel. Implants were inserted in metaphysis of proximal tibiae in all animas bilaterally. In vitro release of SEMA3A was tested using enzyme linked immunosorbent assay. In vitro release of SEMA3A was detectable during the first 10 days, and a burst release of was observed during the first 3 days. No significant difference was observed between Control and Matrigel group. The protective effects of SEMA3A in matrigel on peri-implant bone, implant osseointegration and fixation was confirmed. Compared to matrigel alone, SEMA3A suspended in matrigel increased percent bone volume by 88.7% and 83.3% (p<0.01), bone-to-implant contact ratio by 148.9% (p<0.01), and 24.8% (p<0.05), the maximal push-out force by 149.3% and 209.2% (p<0.01) at 4 and 8 weeks after implant insertion, respectively. Surface modification with SEMA3A suspended in matrigel improved implant osseointegration and fixation in the proximal tibiae of OVX rats. © 2016 Wiley Periodicals, Inc.


He J.,University of Sichuan | He J.,West China Hospital of Stomatology | Li Y.,University of Sichuan | Cao Y.,University of Sichuan | And 5 more authors.
Folia Microbiologica | Year: 2015

As one of the most clinically relevant human habitats, the human mouth is colonized by a set of microorganisms, including bacteria, archaea, fungi, and viruses. Increasing evidence has supported that these microbiota contribute to the two commonest oral diseases of man (dental caries and periodontal diseases), presenting significant risk factors to human health conditions, such as tumor, diabetes mellitus, cardiovascular diseases, bacteremia, preterm birth, and low birth weight in infants. It is widely accepted that oral microorganisms cause diseases mainly by a synergistic or cooperative way, and the interspecies interactions within the oral community play a crucial role in determining whether oral microbiota elicit diseases or not. Since a comprehensive understanding of the complex interspecies interactions within a community needs the knowledge of its endogenous residents, a plenty of research have been carried out to explore the oral microbial diversity. In this review, we focus on the recent progress in this field, including the oral microbiome composition and its association with human diseases. © 2014, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.


Temporomandibular joint (TMJ) ankylosis causes serious problems in oral functions. When it occurs in childhood, it can cause secondary dentofacial deformities. This study described our experience in the simultaneous correction of TMJ ankylosis and secondary dentofacial deformities performed in a single operation.Twenty-seven patients with TMJ ankylosis and secondary deformities were treated in a single surgery from January 2009 to December 2013. Clinical outcomes were assessed based on the oral function, radiography, and medical photography.No relapse (of TMJ ankylosis) occurred in any of the patients during the follow-up period. Oral function and skeletal deformities had (were) significantly improved in all the patients. Most of the patients were satisfied with the final outcome.One-stage treatment permits the simultaneous correction of both the TMJ functional deficit and skeletal deformities in a single operation, which suggests that it is an effective approach for the treatment of patents with TMJ ankylosis and secondary dentofacial deformities.


PubMed | University of Sichuan, Tianjin Stomatological Hospital and West China Hospital of Stomatology
Type: Journal Article | Journal: International endodontic journal | Year: 2015

The aim was to evaluate the incidence and type of defects that occurred with K3 rotary nickel-titanium instruments during routine clinical use.A total of 2397K3 (G-PACKS, SybronEndo, West Collins, Orange, CA, USA) instruments were collected from a graduate endodontic clinic over 21months. All the instruments were limited to a maximum use of 30 canal preparations. The collected instruments were measured by a digital caliper to determine whether any fractures had occurred and then were visually inspected for deformation and fracture under a stereomicroscope. The surfaces of fractured instruments were further evaluated under a scanning electron microscope. Data were analysed using chi-square test and Kruskal-Wallis test.The incidence of instrument defect was 5.63%, consisting of 3.59% fractures and 2.05% deformations. The defect rates of 0.04 and 0.06 files were statistically higher than the other taper groups (P<0.003) except for 0.08 files (P>0.05). For the fractured instruments, 63.95% failed from flexural fatigue, whilst 36.05% failed from torsion.Flexural fracture was the major mode of fracture for instruments with larger taper. A routine check for instrument integrity particularly for 0.04 and 0.06 files at high magnification is recommended after each clinical use.


PubMed | West China Hospital of Stomatology
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

Osteoclasts demineralize and resorb bone once they attach to its surface. However, its still unclear how the osteoclasts choose the specific sites for their attachments. It is postulated in this article that the decreased extracellular free ionized calcium concentration (Ca


PubMed | West China Hospital of Stomatology
Type: | Journal: Oral diseases | Year: 2016

To explore the effects of menstrual cycle on orthodontic pain following initial archwire engagement.Female participants with regular menstrual cycles were recruited and assigned into follicular group or luteal group. Demographical and baseline variables were collected: age, education, menstrual duration, anxiety, oral health impact profile-14 (OHIP-14) and index of complexity outcome and need (ICON). Following initial archwire engagement, orthodontic pain was determined through visual analogue scale (VAS) on 1Finally, 37 and 39 were assigned to the follicular and luteal groups respectively, with balanced demographical and baseline data. Orthodontic pain was significantly affected by menstrual phase and time (both p<0.001), but there was no interaction (p>0.05). Moreover, orthodontic pain was independently predicted by menstrual phase, OHIP, education level and anxiety (all p<0.05).We suggest that practitioners arrange female patients to receive initial archwire engagement during their follicular phases to relieve orthodontic pain. This article is protected by copyright. All rights reserved.

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