Entity

Time filter

Source Type


Peng L.,West China Hospital of Stomatology | Peng L.,University of Sichuan | Dong G.,West China Hospital of Stomatology | Xu P.,West China Hospital of Stomatology | And 6 more authors.
Archives of Oral Biology | Year: 2010

Objective: Wnt5a is generally considered a non-canonical Wnt family member and plays an important role in the development of several tissues through regulation of cell fate, proliferation, migration, polarity and death. This study investigates its expression mode in human tooth development and the involved cell signal transduction pathways, as they remain unclear. Design: The expression of Wnt5a was analyzed by immunohistochemistry method. Recombinant adenovirus encoding full-length Wnt5a cDNA was constructed to investigate four cell signal pathways and nine dentinogenesis nuclear transcription factors in response to Wnt5a in human dental papilla cells (HDPCs). Results: Immunostaining revealed that Wnt5a was expressed in enamel epithelium cells from the bud stage, and in odontoblast layers and dental papilla tissues from early bell stage of human tooth development onward. Western blot analysis indicated that p42/44 MAPK, p38 MAPK, JNK and AKT signal pathways could be phosphorylated by WNT5A. RT-PCR analysis showed that Wnt5a increased the expression of DLX1, DLX2, LEF1, MSX2, PAX9 and RUNX2 mRNA, but decreased BARX1 and PITX2 mRNA. Conclusions: It was concluded that WNT5A is expressed in human tooth development, and that p42/44 MAPK, p38 MAPK, JNK and AKT signal pathways and DLX1, DLX2, LEF1, MSX2, PAX9, RUNX2 could be activated by Wnt5a. Crown Copyright © 2009.


Peng L.,West China Hospital of Stomatology | Peng L.,University of Sichuan | Ren L.B.,West China Hospital of Stomatology | Dong G.,West China Hospital of Stomatology | And 5 more authors.
International Endodontic Journal | Year: 2010

Aim: To investigate the role of Wnt5a in the process of differentiation of human dental papilla cells (HDPCs). Methodology: Recombinant adenovirus encoding full-length Wnt5a cDNA was constructed to investigate the biological role of Wnt5a on the differentiation of HDPCs. The effect of Wnt5a on HDPCs differentiation was determined by ALP activity assay, ALP staining and mineral induction assay. Mineralization-related gene expressions were assessed by RT-PCR. Results: Immunostaining revealed Wnt5a expression in the odontoblast layer and dental papilla tissue. Over-expression of Wnt5a by transfecting HDPCs with an Wnt5a-carrying construct increased ALPase activity and the formation of mineralized nodules of HDPCs. RT-PCR analysis showed that the expressions of mineralization-related genes, such as bone sialoprotein, collagen type I, osteonectin, osteopontin (OCN), dentine matrix protein-1 were up-regulated by Wnt5a. Conclusions: Wnt5a promoted differentiation of HDPCs. © 2010 International Endodontic Journal.

Discover hidden collaborations