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Lin Q.,Wenzhou University | Xu X.,Wenzhou University | Wang B.,Wenzhou University | Shen C.,Wenzhou Institute of Biomaterials and Engineering | And 3 more authors.
Journal of Materials Chemistry B

Posterior capsule opacification (PCO) is a significant complication of intraocular lens (IOL) implantation in cataract surgery, in which the adhesion and proliferation of lens epithelial cells (LECs) on the IOL surface play important roles. In the present study, a highly swollen hyaluronic acid (HA)/chitosan (CHI) polyelectrolyte multilayer was fabricated on the IOL surface via the layer by layer technique. Quartz crystal microbalance with dissipation (QCM-D) results not only show the successful construction of the multilayer, but also indicate its hydrogel-like swollen property. The water content of the (HA/CHI)5 multilayer is around 400%, as obtained by thermogravimetry (TG) analysis. Compared with a pristine IOL, the polysaccharide multilayer modification does not influence its optical property, whereas the adhesion and proliferation of LECs are greatly inhibited. In vivo ocular implantation results show that such a polysaccharide multilayer modification presents good in vivo biocompatibility, and has positive effects on reducing PCO development. © 2015 The Royal Society of Chemistry. Source

Shen C.,Wenzhou University | Shen C.,Wenzhou Institute of Biomaterials and Engineering | Han Y.,Wenzhou University | Wang B.,Wenzhou University | And 3 more authors.
RSC Advances

The tremendous advancement of polyhedral oligomeric silsesquioxanes (POSSs) has been focused on the field of biomaterial applications including tissue engineering, drug delivery, biomedical devices and biosensors. More recently, POSSs have been used in components of ophthalmic biomedical devices, such as contact lenses and intraocular lenses due to their chemical inertness and transparency. A systematic biocompatibility evaluation of POSS nanomaterials is thus essential. Herein, the ocular biocompatibility and cytotoxicity of POSS nanomaterials were investigated both in vitro and in vivo. Three types of commercial POSS nanomaterials with different functional groups were utilized in this research, including aminoethylaminopropylisobutyl-POSS (NH2-POSS), mercaptopropylisobutyl-POSS (SH-POSS) and octahydroxypropyldimethylsilyl-POSS (HO-POSS). The cellular metabolic activity, membrane integrality, cell apoptosis and oxidative damage were tested on human lens epithelial cells (HLECs) under different concentrations of POSS nanomaterial exposure. The ocular irritation on rabbit eyes was measured as well. The results show that the studied POSS nanomaterials do not exhibit any significant toxicity to cell growth and proliferation in most cases, except for the NH2-POSS, which decreases the cellular viability at high concentration. All of the POSS nanomaterials slightly induced oxidative stress as a result of an increase in reactive oxygen species (ROS), however they did not generate cell apoptosis. The animal experiment results also show that no acute ocular irritation can be detected after POSS nanomaterial administration. These results indicate the good ocular biocompatibility of the POSS nanomaterials in most cases, which have great potential in ocular biomedical applications. This journal is © The Royal Society of Chemistry. Source

Yu J.,Wenzhou University | Xu X.,Wenzhou University | Yao F.,Wenzhou University | Yao F.,Wenzhou Institute of Biomaterials and Engineering | And 8 more authors.
International Journal of Pharmaceutics

Avastin® has been clinically proved to be effective in the treatment of intraocular neovascularization diseases. However, the short half-life of Avastin® need frequent administration to maintain its therapeutic efficiency. In this paper, we attempted to develop an in situ PEG hydrogels with great biocompatibility for sustained release of Avastin ® to inhibit the corneal neovascularization. PEG hydrogels was formed via thiol-maleimide reaction using 4-arm PEG-Mal and 4-arm PEG-SH. The transparent hydrogel was rapidly formed under physiological conditions. By varying the concentration of 4-arm PEG-SH, PEG hydrogel with different gelling time, pore size, swelling ratio and mechanical property could be obtained. In vitro cytotoxicity indicated that the developed PEG hydrogel had no apparent cytotoxicity on L-929 cells after 7 days of incubation. In vitro release study showed the encapsulated Avastin® was sustained release from PEG hydrogels within a period of 14 days study. Sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) analysis further confirmed that the released Avastin® did not undergo apparent hydrolysis within 14 days. As a conclusion, we could conclude that the developed PEG hydrogels as an injectable hydrogels might be suitable for extended Avastin® release to treat the corneal neovascularization. © 2014 Elsevier B.V. Source

Shi C.,Tianjin University | Shi C.,Wenzhou Institute of Biomaterials and Engineering | Li Q.,Tianjin University | Zhang W.,Tianjin University | And 4 more authors.
ACS Applied Materials and Interfaces

Herein, we demonstrate that the REDV peptide modified nanoparticles (NPs) can serve as a kind of active targeting gene carrier to condensate pZNF580 for specific promotion of the proliferation of endothelial cells (ECs). First, we synthesized a series of biodegradable amphiphilic copolymers by ring-opening polymerization reaction and graft modification with REDV peptide. Second, we prepared active targeting NPs via self-assembly of the amphiphilic copolymers using nanoprecipitation technology. After condensation with negatively charged pZNF580, the REDV peptide modified NPs/pZNF580 complexes were formed finally. Due to the binding affinity toward ECs of the specific peptide, these REDV peptide modified NPs/pZNF580 complexes could be recognized and adhered specifically by ECs in the coculture system of ECs and human artery smooth muscle cells (SMCs) in vitro. After expression of ZNF580, as the key protein to promote the proliferation of ECs, the relative ZNF580 protein level increased from 15.7% to 34.8%. The specificity in actively targeting ECs of the REDV peptide conjugated NPs/pZNF580 complexes was still retained in the coculture system. These findings in the present study could facilitate the development of actively targeting gene carriers for the endothelialization of artificial blood vessels. © 2015 American Chemical Society. Source

Luo Z.,Wenzhou University | Luo Z.,Wenzhou Institute of Biomaterials and Engineering | Shi S.,Wenzhou University | Jin L.,Wenzhou University | And 5 more authors.
Colloids and Surfaces B: Biointerfaces

Nanoparticles have been proven to be an effective vaccine delivery system that can boost immune responses to subunit vaccines. Herein, we developed and characterized a cationic polymeric polyethylene glycol2000-poly ε-caprolactone2000-polyethylenimine2000 (mPEG2000-PCL2000-g-PEI2000) micelle as a potent vaccine delivery system to boost the immune response in vivo. The micelles that we developed exhibited great antigen-loading capability and minimal cytotoxicity in vitro. Meanwhile, micelles facilitated OVA antigen uptake by dendritic cells both in vitro and in vivo. More importantly, a micelle-formulated OVA vaccine could significantly promote anti-OVA antibody production by 190-fold and potently enhance T cell proliferation and the secretion of IL-5 and IFN-γ. We attributed these effects to its ability to promote antigen uptake, antigen deposition, and germinal center formation. In conclusion, the mPEG2000-PCL2000-PEI2000 micelle that we developed has potential as potent vaccine delivery system to induce Th2 immune response. © 2015 Elsevier B.V. Source

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