Wenzhou Institute of Biomaterials and Engineering

Wenzhou, China

Wenzhou Institute of Biomaterials and Engineering

Wenzhou, China
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Patent
Wenzhou Institute Of Biomaterials And Engineering | Date: 2016-02-01

The present invention discloses a preparation technique of composite nanodots based on carbon nanodots, and their use in the field of fluorescent imaging, wherein, the main components of the composition are carbon nanodots, which are material with superior biocompatibility characteristics, and supporting component is methylene blue, and particle diameter range is 100-500 nanometers, and the zeta potential is 35 to 10 millivolts. The above said techniques for preparation of composite nanodots are safe, quick and simple, low cost, and easy to perform for industrialized production. Composite carbon nanodots have good biocompatibility and safety, high fluorescence imaging sensitivity, and they are promising in gaining wider use in the fields of biomedical imaging, targeting diagnosis and therapy, drug screening and optimization, and in vivo labelling and tracing, and have potential value in personalized medicine.


Luo Z.,Wenzhou University | Jin L.,Wenzhou University | Xu L.,Wenzhou University | Zhang Z.L.,Wenzhou University | And 6 more authors.
Drug Delivery | Year: 2016

Development of efficient ocular drug delivery systems was still a challenging task. The objective of this article was to develop a thermosensitive PEG-PCL-PEG (PECE) hydrogel and investigate its potential application for ocular drug delivery of diclofenac sodium (DIC). PECE block polymers were synthesized by coupling MPEG-PCL co-polymer using IPDI reagent, and then its sol-gel transition as a function with temperature was investigated by a rheometer. The results showed that 30% (w/v) PECE aqueous solution exhibited sol-gel transition at approximately 35 °C. In vitro release profiles showed the entrapped DIC was sustained release from PECE hydrogels up to 7 days and the initial drug loading greatly effect on release behavior of DIC from PECE hydrogels. MTT assay results indicated that no matter PECE or 0.1% (w/v) DIC-loaded PECE hydrogels were nontoxic to HCEC and L929 cells after 24 h culturing. In vivo eye irritation test showed that the instillation of either 30% (w/v) PECE hydrogels or 0.1% (w/v) DIC-loaded PECE hydrogels to rabbit eye did not result in eye irritation within 72 h. In vivo results showed that the AUC0-48 h of 0.1% (w/v) DIC-loaded PECE hydrogels exhibited 1.6-fold increment as compared with that of commercial 0.1% (w/v) DIC eye drops, suggesting the better ophthalmic bioavailability could be obtained by the instillation of 0.1% (w/v) DIC-loaded PECE hydrogels. © 2014 Informa Healthcare USA, Inc.


Chang R.,Northeastern University | Subramanian K.,Northeastern University | Wang M.,Northeastern University | Webster T.J.,Northeastern University | Webster T.J.,Wenzhou Institute of Biomaterials and Engineering
ACS Applied Materials and Interfaces | Year: 2017

The emergence of antibiotic resistance in bacteria has caused many healthcare problems and social burdens. In this study, a type of self-assembled peptide amphiphiles (PA) functionalized with a heparin-binding Cardin-motif peptide (sequence (AKKARK)2) has been designed to combat bacterial drug resistance. Above the critical micelle concentration (CMC) at 45 μM, these amphiphilic Cardin antimicrobial peptide (ACA-PA) can self-assemble into cylindrical supramolecular structures (7-10 nm in diameter) via hydrophobic interactions and β-sheet secondary conformation. The ACA-PA displays excellent antibacterial properties against both Gram-positive and Gram-negative bacteria. This work also demonstrates the effects of molecular self-assembly on antibacterial activity of peptide amphiphiles. The ACA-PA exhibits antibacterial activity on Gram-positive bacteria in a dose-dependent manner, but in the case of Gram-negative bacteria, the antibacterial potency of ACA-PA is remarkably enhanced at concentrations above the CMC. The ACA-PA has been shown to cause bacterial cytoplasmic leakage, causing localized membrane disruption in Gram-positive bacteria and blisters on disorganized membranes of Gram-negative bacteria. Therefore, these peptide-based nanoparticles have promising potential as antimicrobial agents without resorting to the use of antibiotics, and, thus, should be further studied for a wide range of biomaterial applications. © 2017 American Chemical Society.


Shen X.,Nanyang Technological University | Qiu C.,Nanyang Technological University | Qiu C.,Wenzhou Institute of Biomaterials and Engineering | Cao B.,Nanyang Technological University | And 5 more authors.
Nano Research | Year: 2015

In this work, we report the electrical field tuning of magneto-phonon resonance in monolayer graphene under magnetic fields up to 9 T. It is found that the carrier concentration can drastically affect the G (E2g) phonon response to a varying magnetic field through a pronounced magneto-phonon resonance (MPR). In charge neutral or slightly doped monolayer graphene, both the energy and the line width of the E2g phonon show clear variation with magnetic fields. This is attributed to magneto-phonon resonance between magnetoexcitations and the E2g phonons. In contrast, when the Fermi level of the monolayer graphene is far away from the Dirac point, the G band shows weak magnetic dependence and exhibits a symmetric line-shape. This suggests that the magneto-phonon coupling around 4 T has been switched off due to the Pauli blocking of the inter-Landau level excitations. Moreover, the G band asymmetry caused by Fano resonance between excitonic many-body states and the E2g phonons is observed. This work offers a way to study the magnetoexcitation phonon interaction of materials through magneto-Raman spectroscopy with an external electrical field. © 2014, Tsinghua University Press and Springer-Verlag Berlin Heidelberg.


Lin Q.,Wenzhou University | Xu X.,Wenzhou University | Wang B.,Wenzhou University | Shen C.,Wenzhou Institute of Biomaterials and Engineering | And 3 more authors.
Journal of Materials Chemistry B | Year: 2015

Posterior capsule opacification (PCO) is a significant complication of intraocular lens (IOL) implantation in cataract surgery, in which the adhesion and proliferation of lens epithelial cells (LECs) on the IOL surface play important roles. In the present study, a highly swollen hyaluronic acid (HA)/chitosan (CHI) polyelectrolyte multilayer was fabricated on the IOL surface via the layer by layer technique. Quartz crystal microbalance with dissipation (QCM-D) results not only show the successful construction of the multilayer, but also indicate its hydrogel-like swollen property. The water content of the (HA/CHI)5 multilayer is around 400%, as obtained by thermogravimetry (TG) analysis. Compared with a pristine IOL, the polysaccharide multilayer modification does not influence its optical property, whereas the adhesion and proliferation of LECs are greatly inhibited. In vivo ocular implantation results show that such a polysaccharide multilayer modification presents good in vivo biocompatibility, and has positive effects on reducing PCO development. © 2015 The Royal Society of Chemistry.


Wang B.,Wenzhou University | Wang B.,Wenzhou Institute of Biomaterials and Engineering | Lin Q.,Wenzhou University | Lin Q.,Wenzhou Institute of Biomaterials and Engineering | And 5 more authors.
RSC Advances | Year: 2014

Poly(methyl methacrylate) (PMMA) has been widely used for intraocular lenses (IOL) but may lead to posterior capsule opacification (PCO) after implantation due to its undesirable hydrophilicity and surface morphology. A novel methacrylisobutyl polyhedral oligomeric silsesquioxane-co-poly methyl methacrylate copolymer (MA POSS-PMMA) was synthesized by a free radical polymerization method to improve its material properties and cytocompatibility. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and proton nuclear magnetic resonance spectroscopy (1H NMR) measurements demonstrated the successful synthesis of MA POSS-PMMA copolymer. The incorporation of MA POSS greatly changed the crystal structure, surface wettability, optical transmission and cytocompatibility of PMMA. XRD peaks at 2θ ∼ 38.5, 44.7 and 66.1° indicated that a portion of the MA POSS molecules had aggregated and crystallized. Furthermore, larger aggregates are formed at higher MA POSS contents. The optical transmission of the copolymers was up to 99%, which was better than pure PMMA. The hydrophilicity and morphology of the IOL surface were characterized by static water contact angle and atomic force microscopy. Results revealed that MA POSS rendered the surface more hydrophobic and with higher roughness than the pure PMMA. Biocompatibility of copolymers with human lens epithelial cells (HLECs) was further evaluated by morphology and activity measurements in vitro. More HLECs adhesion and better spreading morphology on the surfaces of MA POSS-PMMA copolymers than that on PMMA was shown. © the Partner Organisations 2014.


Wang B.,Wenzhou University | Wang B.,Wenzhou Institute of Biomaterials and Engineering | Lin Q.,Wenzhou University | Lin Q.,Wenzhou Institute of Biomaterials and Engineering | And 5 more authors.
Journal of Colloid and Interface Science | Year: 2014

The development of posterior capsule opacification (PCO) after intraocular lenses (IOL) implantation for dealing with cataract is mainly due to the severe loss of the human lens epithelial cells (HLECs) during surgery contact. A novel poly (hedral oligomeric silsesquioxane-co-methyl methacrylate) copolymer (allyl POSS-PMMA) was synthesized by free radical polymerization method to promote the adhesion of HLECs. FT-IR and 1H NMR measurements indicated the existence of POSS cage in the product, which demonstrated the successful synthesis of allyl POSS-PMMA copolymer. Effect of allyl POSS in the hybrids on crystal structure, surface wettability and morphology, optical transmission, thermodynamic properties and cytocompatibility was investigated in detail. X-ray diffraction peaks at 2θ~11° and 12° indicated that POSS molecules had aggregated and crystallized. Thermogravimetric analysis-differential scanning calorimeter and optical transmission measurements confirmed that the allyl POSS-PMMA copolymer had high glass transition temperatures (more than 100°C) and good transparency. The hydrophilicity and morphology of PMMA and copolymers surfaces were characterized by static water contact angle and atomic force microscopy. The results revealed that the surface of the allyl POSS-PMMA copolymer displayed higher hydrophobicity and higher roughness than that of pure PMMA. The surface biocompatibility was evaluated by morphology and activity measurement with HLECs in vitro. The results verified that the surface of allyl POSS-PMMA copolymer films had more HLECs adhesion and better spreading morphology than that of PMMA film. © 2014 Elsevier Inc.


PubMed | Wenzhou Institute of Biomaterials and Engineering and Tianjin University
Type: Journal Article | Journal: Macromolecular rapid communications | Year: 2016

Gene therapy demonstrates promising prospects on cardiovascular diseases. However, nonviral gene delivery system has relatively low transfection efficiency, especially for endothelial cells (ECs). Herein, typical cell-penetrating peptide (TAT), nuclear localization signals (NLSs), and REDV functional peptide have been used to prepare multitargeting complexes. These complexes exhibit higher transfection efficiency owing to the targeting sequences of REDV and NLSs as well as the cell-penetrating function of TAT. The multifunction of the complexes provides high cell uptake, endo/lysosomal escape, and nucleus accumulation of the encapsulated DNA. Thus these multitargeting complexes can provide a potential platform for gene delivery, especially for EC transfection.


Liang M.,South China University of Technology | Chen H.,Wenzhou Institute of Biomaterials and Engineering | Wei K.,South China University of Technology
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | Year: 2016

Composite microspheres were prepared by an oil-water emulsion and evaporation method, which were then sintered to prepare microsphere scaffolds. The compressive strength, porosity and pore connection rate of the scaffold can be modified by adjusting β-TCP amount, which results a scaffold with compressive strength in the range of cancellous bone, with porosity and pore connection rate large enough to allow cell grow. The surface smoothness decreases with the addition of 0.1, 0.2, 0.3 and 0.4 g β-TCP in PLGA. The adhesion degree decreases and the porosity and average diameter increase with the increase of β-TCP after sintering at 70℃ for 1 h. The average pore diameter is 84.34 μm when adding 0.3 g β-TCP, which is larger than the least diameter (80 μm) for cell grow in scaffolds. Compressive strength increases first and then decreases, which shows the highest value of (6.05 ± 0.74) MPa when adds 0.2 g β-TCP. However, the connection between microspheres decreases with high β-TCP, which results in the decrease of compressive strengths of the scaffolds. Cytocompatibility can be enhanced with the increase of β-TCP. Therefore, an optimum β-TCP amount was found (0.3 g) to prepare microsphere scaffolds with best comprehensive properties. © 2016, Editorial Board of “Journal of Chemical Engineering of Chinese Universities”. All right reserved.


Patent
Wenzhou Institute Of Biomaterials And Engineering | Date: 2015-05-20

A polybutylacrylate-based nanocomposite photoacoustic imaging agent includes a biomedical material such as polybutylacrylate used as a main component and methylene blue or indocyanine green used as an entrapped component with particle diameters of 525.1 nm and 272.0 nm and electric potentials of 4.52 mV and 5.98 mV respectively. The preparation method of the polybutylacrylate-based nanocomposite photoacoustic imaging agent with the features of moderate eco-friendliness, low power consumption, and free of three industrial wastes, radiation, and noise pollution may be used for industrial production.

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