Wenzhou Institute of Biomaterials and Engineering

Wenzhou, China

Wenzhou Institute of Biomaterials and Engineering

Wenzhou, China

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Patent
Wenzhou Institute Of Biomaterials And Engineering | Date: 2016-02-01

The present invention discloses a preparation technique of composite nanodots based on carbon nanodots, and their use in the field of fluorescent imaging, wherein, the main components of the composition are carbon nanodots, which are material with superior biocompatibility characteristics, and supporting component is methylene blue, and particle diameter range is 100-500 nanometers, and the zeta potential is 35 to 10 millivolts. The above said techniques for preparation of composite nanodots are safe, quick and simple, low cost, and easy to perform for industrialized production. Composite carbon nanodots have good biocompatibility and safety, high fluorescence imaging sensitivity, and they are promising in gaining wider use in the fields of biomedical imaging, targeting diagnosis and therapy, drug screening and optimization, and in vivo labelling and tracing, and have potential value in personalized medicine.


Shen X.,Nanyang Technological University | Qiu C.,Nanyang Technological University | Qiu C.,Wenzhou Institute of Biomaterials and Engineering | Cao B.,Nanyang Technological University | And 5 more authors.
Nano Research | Year: 2015

In this work, we report the electrical field tuning of magneto-phonon resonance in monolayer graphene under magnetic fields up to 9 T. It is found that the carrier concentration can drastically affect the G (E2g) phonon response to a varying magnetic field through a pronounced magneto-phonon resonance (MPR). In charge neutral or slightly doped monolayer graphene, both the energy and the line width of the E2g phonon show clear variation with magnetic fields. This is attributed to magneto-phonon resonance between magnetoexcitations and the E2g phonons. In contrast, when the Fermi level of the monolayer graphene is far away from the Dirac point, the G band shows weak magnetic dependence and exhibits a symmetric line-shape. This suggests that the magneto-phonon coupling around 4 T has been switched off due to the Pauli blocking of the inter-Landau level excitations. Moreover, the G band asymmetry caused by Fano resonance between excitonic many-body states and the E2g phonons is observed. This work offers a way to study the magnetoexcitation phonon interaction of materials through magneto-Raman spectroscopy with an external electrical field. © 2014, Tsinghua University Press and Springer-Verlag Berlin Heidelberg.


Lin Q.,Wenzhou University | Xu X.,Wenzhou University | Wang B.,Wenzhou University | Shen C.,Wenzhou Institute of Biomaterials and Engineering | And 3 more authors.
Journal of Materials Chemistry B | Year: 2015

Posterior capsule opacification (PCO) is a significant complication of intraocular lens (IOL) implantation in cataract surgery, in which the adhesion and proliferation of lens epithelial cells (LECs) on the IOL surface play important roles. In the present study, a highly swollen hyaluronic acid (HA)/chitosan (CHI) polyelectrolyte multilayer was fabricated on the IOL surface via the layer by layer technique. Quartz crystal microbalance with dissipation (QCM-D) results not only show the successful construction of the multilayer, but also indicate its hydrogel-like swollen property. The water content of the (HA/CHI)5 multilayer is around 400%, as obtained by thermogravimetry (TG) analysis. Compared with a pristine IOL, the polysaccharide multilayer modification does not influence its optical property, whereas the adhesion and proliferation of LECs are greatly inhibited. In vivo ocular implantation results show that such a polysaccharide multilayer modification presents good in vivo biocompatibility, and has positive effects on reducing PCO development. © 2015 The Royal Society of Chemistry.


Wang B.,Wenzhou University | Wang B.,Wenzhou Institute of Biomaterials and Engineering | Lin Q.,Wenzhou University | Lin Q.,Wenzhou Institute of Biomaterials and Engineering | And 5 more authors.
RSC Advances | Year: 2014

Poly(methyl methacrylate) (PMMA) has been widely used for intraocular lenses (IOL) but may lead to posterior capsule opacification (PCO) after implantation due to its undesirable hydrophilicity and surface morphology. A novel methacrylisobutyl polyhedral oligomeric silsesquioxane-co-poly methyl methacrylate copolymer (MA POSS-PMMA) was synthesized by a free radical polymerization method to improve its material properties and cytocompatibility. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and proton nuclear magnetic resonance spectroscopy (1H NMR) measurements demonstrated the successful synthesis of MA POSS-PMMA copolymer. The incorporation of MA POSS greatly changed the crystal structure, surface wettability, optical transmission and cytocompatibility of PMMA. XRD peaks at 2θ ∼ 38.5, 44.7 and 66.1° indicated that a portion of the MA POSS molecules had aggregated and crystallized. Furthermore, larger aggregates are formed at higher MA POSS contents. The optical transmission of the copolymers was up to 99%, which was better than pure PMMA. The hydrophilicity and morphology of the IOL surface were characterized by static water contact angle and atomic force microscopy. Results revealed that MA POSS rendered the surface more hydrophobic and with higher roughness than the pure PMMA. Biocompatibility of copolymers with human lens epithelial cells (HLECs) was further evaluated by morphology and activity measurements in vitro. More HLECs adhesion and better spreading morphology on the surfaces of MA POSS-PMMA copolymers than that on PMMA was shown. © the Partner Organisations 2014.


Wang B.,Wenzhou University | Wang B.,Wenzhou Institute of Biomaterials and Engineering | Lin Q.,Wenzhou University | Lin Q.,Wenzhou Institute of Biomaterials and Engineering | And 5 more authors.
Journal of Colloid and Interface Science | Year: 2014

The development of posterior capsule opacification (PCO) after intraocular lenses (IOL) implantation for dealing with cataract is mainly due to the severe loss of the human lens epithelial cells (HLECs) during surgery contact. A novel poly (hedral oligomeric silsesquioxane-co-methyl methacrylate) copolymer (allyl POSS-PMMA) was synthesized by free radical polymerization method to promote the adhesion of HLECs. FT-IR and 1H NMR measurements indicated the existence of POSS cage in the product, which demonstrated the successful synthesis of allyl POSS-PMMA copolymer. Effect of allyl POSS in the hybrids on crystal structure, surface wettability and morphology, optical transmission, thermodynamic properties and cytocompatibility was investigated in detail. X-ray diffraction peaks at 2θ~11° and 12° indicated that POSS molecules had aggregated and crystallized. Thermogravimetric analysis-differential scanning calorimeter and optical transmission measurements confirmed that the allyl POSS-PMMA copolymer had high glass transition temperatures (more than 100°C) and good transparency. The hydrophilicity and morphology of PMMA and copolymers surfaces were characterized by static water contact angle and atomic force microscopy. The results revealed that the surface of the allyl POSS-PMMA copolymer displayed higher hydrophobicity and higher roughness than that of pure PMMA. The surface biocompatibility was evaluated by morphology and activity measurement with HLECs in vitro. The results verified that the surface of allyl POSS-PMMA copolymer films had more HLECs adhesion and better spreading morphology than that of PMMA film. © 2014 Elsevier Inc.


Yu J.,Wenzhou University | Xu X.,Wenzhou University | Yao F.,Wenzhou University | Yao F.,Wenzhou Institute of Biomaterials and Engineering | And 8 more authors.
International Journal of Pharmaceutics | Year: 2014

Avastin® has been clinically proved to be effective in the treatment of intraocular neovascularization diseases. However, the short half-life of Avastin® need frequent administration to maintain its therapeutic efficiency. In this paper, we attempted to develop an in situ PEG hydrogels with great biocompatibility for sustained release of Avastin ® to inhibit the corneal neovascularization. PEG hydrogels was formed via thiol-maleimide reaction using 4-arm PEG-Mal and 4-arm PEG-SH. The transparent hydrogel was rapidly formed under physiological conditions. By varying the concentration of 4-arm PEG-SH, PEG hydrogel with different gelling time, pore size, swelling ratio and mechanical property could be obtained. In vitro cytotoxicity indicated that the developed PEG hydrogel had no apparent cytotoxicity on L-929 cells after 7 days of incubation. In vitro release study showed the encapsulated Avastin® was sustained release from PEG hydrogels within a period of 14 days study. Sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) analysis further confirmed that the released Avastin® did not undergo apparent hydrolysis within 14 days. As a conclusion, we could conclude that the developed PEG hydrogels as an injectable hydrogels might be suitable for extended Avastin® release to treat the corneal neovascularization. © 2014 Elsevier B.V.


PubMed | Wenzhou University and Wenzhou Institute of Biomaterials and Engineering
Type: | Journal: Journal of colloid and interface science | Year: 2014

The development of posterior capsule opacification (PCO) after intraocular lenses (IOL) implantation for dealing with cataract is mainly due to the severe loss of the human lens epithelial cells (HLECs) during surgery contact. A novel poly (hedral oligomeric silsesquioxane-co-methyl methacrylate) copolymer (allyl POSS-PMMA) was synthesized by free radical polymerization method to promote the adhesion of HLECs. FT-IR and (1)H NMR measurements indicated the existence of POSS cage in the product, which demonstrated the successful synthesis of allyl POSS-PMMA copolymer. Effect of allyl POSS in the hybrids on crystal structure, surface wettability and morphology, optical transmission, thermodynamic properties and cytocompatibility was investigated in detail. X-ray diffraction peaks at 211 and 12 indicated that POSS molecules had aggregated and crystallized. Thermogravimetric analysis-differential scanning calorimeter and optical transmission measurements confirmed that the allyl POSS-PMMA copolymer had high glass transition temperatures (more than 100C) and good transparency. The hydrophilicity and morphology of PMMA and copolymers surfaces were characterized by static water contact angle and atomic force microscopy. The results revealed that the surface of the allyl POSS-PMMA copolymer displayed higher hydrophobicity and higher roughness than that of pure PMMA. The surface biocompatibility was evaluated by morphology and activity measurement with HLECs in vitro. The results verified that the surface of allyl POSS-PMMA copolymer films had more HLECs adhesion and better spreading morphology than that of PMMA film.


PubMed | Wenzhou Institute of Biomaterials and Engineering and Tianjin University
Type: Journal Article | Journal: Macromolecular rapid communications | Year: 2016

Gene therapy demonstrates promising prospects on cardiovascular diseases. However, nonviral gene delivery system has relatively low transfection efficiency, especially for endothelial cells (ECs). Herein, typical cell-penetrating peptide (TAT), nuclear localization signals (NLSs), and REDV functional peptide have been used to prepare multitargeting complexes. These complexes exhibit higher transfection efficiency owing to the targeting sequences of REDV and NLSs as well as the cell-penetrating function of TAT. The multifunction of the complexes provides high cell uptake, endo/lysosomal escape, and nucleus accumulation of the encapsulated DNA. Thus these multitargeting complexes can provide a potential platform for gene delivery, especially for EC transfection.


Liang M.,South China University of Technology | Chen H.,Wenzhou Institute of Biomaterials and Engineering | Wei K.,South China University of Technology
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | Year: 2016

Composite microspheres were prepared by an oil-water emulsion and evaporation method, which were then sintered to prepare microsphere scaffolds. The compressive strength, porosity and pore connection rate of the scaffold can be modified by adjusting β-TCP amount, which results a scaffold with compressive strength in the range of cancellous bone, with porosity and pore connection rate large enough to allow cell grow. The surface smoothness decreases with the addition of 0.1, 0.2, 0.3 and 0.4 g β-TCP in PLGA. The adhesion degree decreases and the porosity and average diameter increase with the increase of β-TCP after sintering at 70℃ for 1 h. The average pore diameter is 84.34 μm when adding 0.3 g β-TCP, which is larger than the least diameter (80 μm) for cell grow in scaffolds. Compressive strength increases first and then decreases, which shows the highest value of (6.05 ± 0.74) MPa when adds 0.2 g β-TCP. However, the connection between microspheres decreases with high β-TCP, which results in the decrease of compressive strengths of the scaffolds. Cytocompatibility can be enhanced with the increase of β-TCP. Therefore, an optimum β-TCP amount was found (0.3 g) to prepare microsphere scaffolds with best comprehensive properties. © 2016, Editorial Board of “Journal of Chemical Engineering of Chinese Universities”. All right reserved.


Patent
Wenzhou Institute Of Biomaterials And Engineering | Date: 2015-05-20

A polybutylacrylate-based nanocomposite photoacoustic imaging agent includes a biomedical material such as polybutylacrylate used as a main component and methylene blue or indocyanine green used as an entrapped component with particle diameters of 525.1 nm and 272.0 nm and electric potentials of 4.52 mV and 5.98 mV respectively. The preparation method of the polybutylacrylate-based nanocomposite photoacoustic imaging agent with the features of moderate eco-friendliness, low power consumption, and free of three industrial wastes, radiation, and noise pollution may be used for industrial production.

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