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Dai T.,Wenzhou Medical College | Zhao E.,Wenzhou Medical College | Zhao E.,Lishui Peoples Hospital | Lu G.,Wenzhou Medical College | And 9 more authors.
Aquaculture | Year: 2012

This study was intended to develop an optimal sperm cryopreservation protocol for yellow drum (Nibea albiflora), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56-61%) and hatch (42-47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220°C/min, thawed in 40°C water bath for 7s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10 7. We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these changes was small when compared to the 2000 to 3000-fold reduction in fertilization/hatch capability of thawed sperm. These findings suggest possible disruption of signaling pathways that cannot be detected by conventional quality assays. Further analysis of sperm membrane lipid composition revealed a significant negative correlation between sperm quality and cholesterol-to-sphingomyelin ratio. These findings indicate that lipid rafts could be the main targets for cryodamage-induced decrease in fertilization capability of thawed sperm. Future studies are necessary to further explore the role of lipid rafts in cryopreservation-mediated damage in fish sperm. © 2012 Elsevier B.V.


He Q.,Wenzhou Medical College | Zhao E.,Wenzhou Medical College | Lu Y.,Wenzhou Fisheries Technology Extension Service | Yan M.,Zhejiang Metallurgical Research Institute | And 2 more authors.
Aquaculture | Year: 2012

The yellow drum (Nibea albiflora) is a commercially important fish species in East Asia and artificial propagation has recently been initiated due to the rapid decrease of wild capture. To facilitate artificial insemination in aquaculture, sperm activation and storage conditions were evaluated in this study. Our findings revealed that yellow drum sperm can be activated with calcium-free Hank[U+A78C]s balanced salt solution (C-F HBSS), KCl, NaCl, and glucose at an osmolality range of 300-1200 mOsm/kg with the highest motility observed at 600-800 mOsm/kg. The average motility duration was less than 6 min. For sperm storage, C-F HBSS, fish Ringer solution, KCl, NaCl, and glucose at 150, 270, and 300 mOsm/kg were compared for selection of the optimal extender, and results showed detrimental effects of KCl, NaCl, and glucose. In contrast, C-F HBSS and fish Ringer solution or sperm stored undiluted in seminal plasma could retain motility for up to 48 h when samples were stored at 4 °C. The highest sperm motility was found with treatments at 150 mOsm/kg for both C-F HBSS and fish Ringer solution. Sperm samples stored at 4 °C were also found to retain motility longer than those stored at room temperature (25 °C). Our findings provide the basic information on sperm handling in yellow drum. © 2011 Elsevier B.V.


Lu L.-L.,Wenzhou Fisheries Technology Extension Service | Zhang Y.-P.,Wenzhou University | Li K.,Wenzhou Fisheries Technology Extension Service | Hu R.-Y.,Wenzhou University | And 3 more authors.
Chinese Journal of Ecology | Year: 2011

Investigations were made on the macrobenthos diversity in intertidal zone of Dazhuyu Island in April 2009 (spring), July 2009 (summer), October 2009 (autumn), and January 2010 (winter). A total of 125 macrobenthos species were collected, including 57 mollusca, 24 crustacea, 6 coelenterates, 6 other animals, and 32 macroalgae, accounting for 45.6%, 19.2%, 4.8%, 4.8%, and 25.6% of the total, respectively. The dominant species in high, mid, and low tidal zones were Nodilittorina exigua and Littorina brevicula, Tetraclita japonica and Septifer virgatus, and Hydroides sp., Sargassum thunbergii, and Lithophaga curta, respectively. The macrobenthos abundance was averagely 1637 ind·m-2, and the biomass was 2851.63g· m-2 on average. The Shannon index, Margalef index, and Pielou evenness index were 1.63, 3.36, and 0.35, respectively. There was a consistency between the results of hierarchical cluste. ring and non-metric multidimensional scaling analysis. The abundance/ biomass curve showed that the community structure of macrobenthos in the intertidal zone of Dazhuyu Island had been disturbed, but the community was relatively steady.


Jihan C.,Wenzhou Fisheries Technology Extension Service | Yonglin L.,Wenzhou Fisheries Technology Extension Service | Peng C.,Yueqing Ocean and Fishery Environmental Monitoring Station | Kai L.,Wenzhou Fisheries Technology Extension Service | And 2 more authors.
Aquaculture Research | Year: 2015

To control heavy metal pollution during the culture of the mud clam, Tegillarca granosa L., the isothermal static adsorption capacities of three adsorption materials [natural zeolites, zeolites synthesized from kaolin and zeolites synthesized from coal fly ash (ZFA)] towards Cu2+, Cd2+, Cr6+, Zn2+, Pb2+ and Hg2+ in seawater were investigated. The adsorption capacity of the best material was investigated during the culture of mud clam in an artificially polluted environment. Results showed that the ZFA adsorption capacity was the highest and conformed to the Freundlich model. The theoretical maximum adsorption capacities of the adsorbent towards Cu2+, Cd2+, Cr6+, Zn2+, Pb2+ and Hg2+ in seawater were 3.057, 1.123, 0.325, 13.101, 6.116 mg g-1 and 6.527 μg g-1 respectively. For seawater artificially polluted by six heavy metals, heavy metal adsorption by ZFA followed the order Zn2+ > Cd2+ > Hg2+ > Cu2+ > Pb2+ > Cr6+. For the mud clam culturing experiment that simulated a continuously polluted water source, the Cu2+, Cd2+, Cr6+, Zn2+, Pb2+ and Hg2+ contents of mud clam tissues decreased by 40.9%, 25.8%, 9.6%, 28.2%, -27.8% and 25.7%, respectively, after 21 days of culturing compared with the contents before the experiment. © 2013 John Wiley & Sons Ltd.

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