Wenzhou Academy of Agricultural science
Wenzhou Academy of Agricultural science
Bai Y.,Wenzhou Academy of Agricultural science |
Hu J.,Wenzhou Vocational and Scientific College |
Liu S.,Wenzhou Academy of Agricultural science |
Zhang W.,Wenzhou Academy of Agricultural science |
And 6 more authors.
Food and Agricultural Immunology | Year: 2017
The residue of 17β-estradiol (E2) in milk could potentially lead to the occurrence of various reproductive diseases; therefore, a rapid and sensitive method for monitoring E2 residues in milk was highly necessary. In this study, we produced new polyclonal and monoclonal antibodies using E2-3-O-carboxymethyl ether as a hapten and developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the detection of E2 in milk. The results showed that the sensitivity of polyclonal antibody was higher than that of monoclonal antibody, providing a half maximum inhibition concentration (IC50) against E2 of 0.17 ng/mL, high cross-reactivity (CR) to E2 benzoate (150%) and oestriol (18.02%), and negligible CR with other oestrogen compounds. Under optimized conditions, the developed icELISA based on the polyclonal antibody had a limit of detection values of 0.093 μg/L, which was enough sensitive to detect E2 in milk. In spiked samples (0.5, 1, and 2 μg/L), the recoveries ranged from 83.12% to 94.58% with coefficients of variation <12.8%. These results indicated that the icELISA method we developed was suitable for screening of E2 residue in milk. © 2017 Informa UK Limited, trading as Taylor & Francis Group
Chen J.,China Three Gorges University |
Lu X.,Wenzhou Academy of Agricultural science
Chinese Journal of Environmental Engineering | Year: 2013
The indigenous microbial promotion is an in situ black-odor sediment remediation technology with low cost and high efficiency which is applied to river or lake. However, the adding of agent to the sediment may affects the quality of overlying water. In order to analyze the adverse effects of this technology, we determined the changes of phosphorus (P) contents and its fractions in the overlying water and black-odor sediment from an urban lake. Results showed that when the adding dose of Bio Energizer (BE) and Micatrol (MT) to sediment was 60 mL/m3 and 70 mL/m3, respectively, the total phosphorus content (TP) in the overlying water was lower than 0.05 mg/L (Grade III value of National Environment Quality Standard for Surface Water in China). However, the adding of microbial nutrient (BE and MT) to sediment still led to the increase of TP as well as the proliferation of algae in the overlying water. Adding of Ca(NO3)2 significantly reduced the TP and algae biomass in the overlying water, which controlled the effects of adding microbial nutrient to sediment on the TP in overlying water. In addition, the adding of both Ca(NO3)2 and microbial nutrient had no obvious effect on the TP and P fractions in the sediment.
Liu J.,Zhejiang University |
Li M.,Zhejiang University |
Li J.-M.,Zhejiang University |
Huang C.-J.,Zhejiang University |
And 3 more authors.
Journal of Zhejiang University: Science B | Year: 2010
The ecological effects of plant-virus-vector interactions on invasion of alien plant viral vectors have been rarely investigated. We examined the transmission of Tomato yellow leaf curl China virus (TYLCCNV) by the invasive Q biotype and the indigenous ZHJ2 biotype of the whitefly Bemisia tabaci, a plant viral vector, as well as the influence of TYLCCNV-infection of plants on the performance of the two whitefly biotypes. Both whitefly biotypes were able to acquire viruses from infected plants and retained them in their bodies, but were unable to transmit them to either tobacco or tomato plants. However, when the Q biotype fed on tobacco plants infected with TYLCCNV, its fecundity and longevity were increased by 7- and 1-fold, respectively, compared to those of the Q biotype fed on uninfected tobacco plants. When the ZHJ2 biotype fed on virus-infected plants, its fecundity and longevity were increased by only 2- and 0.5-fold, respectively. These data show that the Q biotype acquired higher beneficial effects from TYLCCNV-infection of tobacco plants than the ZHJ2 biotype. Thus, the Q biotype whitefly may have advantages in its invasion and displacement of the indigenous ZHJ2 biotype. © 2010 Zhejiang University and Springer Berlin Heidelberg.
Ali S.,Zhejiang University |
Bai P.,Wenzhou Academy of Agricultural science |
Zeng F.,Zhejiang University |
Cai S.,Zhejiang University |
And 4 more authors.
Environmental and Experimental Botany | Year: 2011
The effects of single or combined stress of aluminum (Al) and chromium (Cr) on plant growth, root dehydrogenase, oxidative stress and antioxidative enzymes were studied using two barley genotypes differing in Al tolerance in a hydroponic experiment. Al or Cr stress decreased plant growth, lowered root dehydrogenase activity and caused oxidative damage, as characterized by increased MDA and H 2O 2 contents. Under Al or Cr stress, the activities of antioxidative enzymes, including superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR) and catalase (CAT), were dramatically increased in plant tissues. Gebeina, an Al-tolerant genotype, had less oxidative damage than Shang 70-119, an Al-sensitive genotype. The extent of oxidative damage induced by Cr varied with the pH of the culture solution, with lower pH values (4.0) being more severe than higher pH values (6.5). The combination of Cr and Al caused a further decrease in plant growth, a decrease in root dehydrogenase activity and an increase in MDA and H 2O 2 contents as well as the activities of antioxidative enzymes. There was also a marked difference between the two barley genotypes in the extent of increased antioxidative enzyme activity under the Cr and Al stresses. © 2010 Elsevier B.V.
Wang Y.,Wenzhou Academy of Agricultural science |
Li X.,Nanjing Agricultural University |
Xu J.,Nanjing Agricultural University |
Xu Y.,Nanjing Agricultural University |
Liu L.,Nanjing Agricultural University
Acta Horticulturae | Year: 2011
Cauliflower is one of the most popular vegetable crops worldwide. In this study, three molecular marker systems including RAPD, ISSR and SRAP were employed to identify cultivars and the relationship of 29 cauliflower cultivars, one broccoli and one cabbage cultivar. The results suggested that nine RAPD primers amplified 98 distinct bands in the 32 cultivars and the polymorphic rate was 78.57%, 10 ISSR primers amplified 91 bands and the polymorphic rate was 63.74%, seven SRAP primers amplified 112 bands and the polymorphic rate was 78.57%, indicating that high polymorphism existed in 32 cultivars. Out of all the primers, the primer me1-em4 produced 17 polymorphic bands and could distinguish 24 cultivars, while the primer me8-em8 produced 17 polymorphic bands and could distinguish 27 cultivars. 32 cultivars could be distinguished with these two primers. Cluster analysis indicated that the genetic relationship of these cauliflower cultivars was tightly associated with its origin and characters. It could be concluded that RAPD, ISSR and SRAP markers were powerful for cultivar identification and genetic diversity analysis in cauliflower.