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Chen J.,China Three Gorges University | Lu X.,Wenzhou Academy of Agricultural science
Chinese Journal of Environmental Engineering | Year: 2013

The indigenous microbial promotion is an in situ black-odor sediment remediation technology with low cost and high efficiency which is applied to river or lake. However, the adding of agent to the sediment may affects the quality of overlying water. In order to analyze the adverse effects of this technology, we determined the changes of phosphorus (P) contents and its fractions in the overlying water and black-odor sediment from an urban lake. Results showed that when the adding dose of Bio Energizer (BE) and Micatrol (MT) to sediment was 60 mL/m3 and 70 mL/m3, respectively, the total phosphorus content (TP) in the overlying water was lower than 0.05 mg/L (Grade III value of National Environment Quality Standard for Surface Water in China). However, the adding of microbial nutrient (BE and MT) to sediment still led to the increase of TP as well as the proliferation of algae in the overlying water. Adding of Ca(NO3)2 significantly reduced the TP and algae biomass in the overlying water, which controlled the effects of adding microbial nutrient to sediment on the TP in overlying water. In addition, the adding of both Ca(NO3)2 and microbial nutrient had no obvious effect on the TP and P fractions in the sediment. Source

Liu J.,Zhejiang University | Li M.,Zhejiang University | Li J.-M.,Zhejiang University | Huang C.-J.,Zhejiang University | And 3 more authors.
Journal of Zhejiang University: Science B | Year: 2010

The ecological effects of plant-virus-vector interactions on invasion of alien plant viral vectors have been rarely investigated. We examined the transmission of Tomato yellow leaf curl China virus (TYLCCNV) by the invasive Q biotype and the indigenous ZHJ2 biotype of the whitefly Bemisia tabaci, a plant viral vector, as well as the influence of TYLCCNV-infection of plants on the performance of the two whitefly biotypes. Both whitefly biotypes were able to acquire viruses from infected plants and retained them in their bodies, but were unable to transmit them to either tobacco or tomato plants. However, when the Q biotype fed on tobacco plants infected with TYLCCNV, its fecundity and longevity were increased by 7- and 1-fold, respectively, compared to those of the Q biotype fed on uninfected tobacco plants. When the ZHJ2 biotype fed on virus-infected plants, its fecundity and longevity were increased by only 2- and 0.5-fold, respectively. These data show that the Q biotype acquired higher beneficial effects from TYLCCNV-infection of tobacco plants than the ZHJ2 biotype. Thus, the Q biotype whitefly may have advantages in its invasion and displacement of the indigenous ZHJ2 biotype. © 2010 Zhejiang University and Springer Berlin Heidelberg. Source

Wang Y.,Wenzhou Academy of Agricultural science | Li X.,Nanjing Agricultural University | Xu J.,Nanjing Agricultural University | Xu Y.,Nanjing Agricultural University | Liu L.,Nanjing Agricultural University
Acta Horticulturae | Year: 2011

Cauliflower is one of the most popular vegetable crops worldwide. In this study, three molecular marker systems including RAPD, ISSR and SRAP were employed to identify cultivars and the relationship of 29 cauliflower cultivars, one broccoli and one cabbage cultivar. The results suggested that nine RAPD primers amplified 98 distinct bands in the 32 cultivars and the polymorphic rate was 78.57%, 10 ISSR primers amplified 91 bands and the polymorphic rate was 63.74%, seven SRAP primers amplified 112 bands and the polymorphic rate was 78.57%, indicating that high polymorphism existed in 32 cultivars. Out of all the primers, the primer me1-em4 produced 17 polymorphic bands and could distinguish 24 cultivars, while the primer me8-em8 produced 17 polymorphic bands and could distinguish 27 cultivars. 32 cultivars could be distinguished with these two primers. Cluster analysis indicated that the genetic relationship of these cauliflower cultivars was tightly associated with its origin and characters. It could be concluded that RAPD, ISSR and SRAP markers were powerful for cultivar identification and genetic diversity analysis in cauliflower. Source

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