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Liu H.,CAS Institute of Botany | Liu H.,University of Chinese Academy of Sciences | Xia T.,CAS Institute of Botany | Xia T.,University of Chinese Academy of Sciences | And 3 more authors.
American Journal of Botany | Year: 2011

Premise of the study: Microsatellite primers were developed for a Chinese traditional herb, Panax notoginseng, to investigate its genetic diversity and cultivar breeding. Methods and Results: Twelve polymorphic microsatellite loci were isolated from the microsatellite-enriched genomic library of Panax notoginseng. The polymorphisms were assessed in two populations and an assemblage containing individuals from the entire distribution area. The number of alleles per locus ranged from 3 to 12, with a mean of 5.8; the observed and expected heterozygosity values ranged from 0.0411 to 0.8472 and from 0.0804 to 0.7653, respectively. Conclusions: These new microsatellite markers will be useful for investigating the genetic diversity of this cultivated Panax notoginseng as well as assist in cultivar breeding. © 2011 Botanical Society of America.

Zuo Y.,CAS Institute of Botany | Zuo Y.,University of Chinese Academy of Sciences | Chen Z.,Wenshan Institute of Sanqi Research | Kondo K.,Tokyo University of Agriculture | And 3 more authors.
Planta Medica | Year: 2011

Ginsengs (Panax, Araliaceae) are among the plants best known for their medicinal properties. Many ginseng species are endangered due to over-exploitation of natural resources - a situation difficult to remedy while there are no reliable, practical methods for species identification. We screened eleven candidate DNA barcoding loci to establish an accurate and effective Panax species identification system, both for commercial and conservation purposes. We used 95 ginseng samples, representing all the species in the genus. We found considerable differences in the performance of the potential barcoding regions. The sequencing of atpF-atpH was unsuccessful due to poly-N structures. The rbcL, rpoB, and rpoC1 regions were found to be mostly invariable, with only four to eight variable sites. Using matK, psbKI, psbM-trnD, rps16 and nad1, we could identify four to six out of eight considerably divergent species but only one to five out of nineteen clusters within the P. bipinnatifidus species group. psbA-trnH and ITS were the most variable loci, working very well both in species and cluster identifications. We demonstrated that the combination of psbA-trnH and ITS is sufficient for identifying all the species and clusters in the genus. © Georg Thieme Verlag KG Stuttgart New York.

Yan X.L.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | Lin L.Y.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | Lin L.Y.,China University of Mining and Technology | Liao X.Y.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | And 2 more authors.
Chemosphere | Year: 2013

Panax notoginseng (Burk.) F.H. Chen, a rare traditional Chinese medicinal herb, is a widely used phytomedicine used all over the world. In recent years, the arsenic contamination of the herb and its relative products becomes a serious problem due to elevated soil As concentration. This study aimed to evaluate the effects of different types and dosages of amendments on As stabilization in soil and its uptake by P. notoginseng. Results showed that comparing to control treatment, the As concentrations of P. notoginseng declined by 49-63%, 43-61% and 52-66% in 0.25% zero-valent iron (Fe(0)), 0.5% bauxite residue, and 1% zeolite treatment, respectively; whereas the biomasses were elevated by 62-116%, 45-152% and 114-265%, respectively. The As(III) proportions of P. notoginseng increased by 8%, 9%, and 8%, and the transfer factors of As from root to shoot increased by 37%, 42% and 84% in the optimal treatments of Fe(0), bauxite residue, and zeolite. For soil As, all the three amendments could transform the non-specifically adsorbed As fraction to hydrous oxides Fe/Al fractions (by Fe(0) and red mud) or specifically adsorbed As fraction (by zeolite), therefore reduced the bioavailability of soil As. With a comprehensive consideration of stabilization efficiency, plant growth, environmental influence, and cost, Fe(0) appeared to be the best amendment, and zeolite could also be a good choice. In conclusion, this study was of significance in developing As contamination control in P. notoginseng planting areas, and even other areas for medicinal herb growing. © 2013 Elsevier Ltd.

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