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Lin X.-Y.,Shandong University | Li Y.-M.,Wendeng Central Hospital of Weihai | Song Y.-H.,Shandong University | Song J.-Q.,Shandong University | And 2 more authors.
Chinese Journal of Contemporary Neurology and Neurosurgery

Objective: To investigate the clinical manifestations and pathological characteristics of pulmonary papillary adenocarcinoma with psammoma bodies metastasis to right frontal lobe of brain. Methods: The clinical manifestations, pathological features and differential diagnosis were studied in one case of pulmonary papillary adenocarcinoma with psammoma bodies metastasis to right frontal lobe. Related literatures were also reviewed. Results: A 56-year-old male suffered from intermittent headache for 3 years in fronto-occipital region without significant incentives. Cranial MRI examination revealed a space-occupying lesion in right frontal lobe, which was considered as meningioma. In operation, the tumor located in the right frontal lobe was tough and red-grey in color, with clear boundary and abundant blood supply. Histopathological examination revealed the tumor was arranged in papillary pattern. The tumor cells were large, with eosinophilic cytoplasm, round or oval nuclei and small red nucleoli. Especially, a plenty of psammoma bodies were found in tumor tissue and mesenchymal tissue. Immunohistochemical staining found that the tumor cells were positively expressed for cytokeratin (CK), epithelial membrane antigen (EMA), CK19, thyroid transcription factor-1 (TTF-1) and Napsin A, and showed focal positive expression of P53. Ki-67 labeling index was 5%-10%. The tumor cells were negative for glial fibrillary acidic protein (GFAP), S-100 protein (S-100), thyroglobulin (TG), Galectin-3 protein, progestrone receptor (PR) and vimentin (Vim). Combined with the results of chest 18F-FDG-PET CT, the final diagnosis was pulmonary papillary adenocarcinoma with psammoma bodies metastasis to right frontal lobe. The patient underwent auxiliary radiotherapy and chemotherapy after operation. The patient was followed for 12 months and no recurrence was seen. Conclusions: This case is very rare. Understanding the patient's history and differentiating from other primary intracalvarium tumors is one of the key steps to give the right pathological diagnosis and clinical therapy. In order to avoid misdiagnosis, the diagnosis needs to be differentiated from other primary intracalvarium tumors, including papillary meningioma, papillary tumor of choroid plexus, papillary ependymoma, papillary glioneuronal tumors as well as metastatic papillary thyroid carcinoma. ©, 2015, Tianjin Huanhu Hospital. All right reserved. Source

Lu L.,Linyi Peoples Hospital | Zhan Y.,Fudan University | Yu J.,Fudan University | Sui L.,Wendeng Central Hospital of Weihai
Biological Trace Element Research

The present study aimed to investigate the prevalence of thrombocytopenia and its association with serum magnesium in a nationally representative cohort. A total of 8478 participants aged 18 years and over were recruited in a cross-sectional survey. Thrombocytopenia was defined as platelet count less than 150 × 109/L. Multivariable logistic regression models were applied to examine the association between serum magnesium and thrombocytopenia. The prevalence of thrombocytopenia in total was 16.5 % with 18.8 % for men and 14.4 % for women (P < 0.0001), respectively. Compared with men in the first quartile of serum magnesium, the odds ratios (ORs) and 95 % confidence intervals (CIs) for those in the second, third, and fourth quartiles of serum magnesium were 0.96 (0.75, 1.21), 0.78 (0.62, 0.98), and 0.82 (0.65, 1.04), respectively, after adjusting for multiple confounders. Likewise, the corresponding ORs (95 % CIs) were 0.80 (0.63, 1.01), 0.79 (0.62, 0.99), and 0.65 (0.51, 0.84) in women. When serum magnesium was treated as a continuous variable, each one standard deviation increase of magnesium was associated with 12 and 8 % lower risk of thrombocytopenia in men and women, respectively. Serum magnesium was inversely associated with thrombocytopenia, and the association was slightly different in men compared with that in women. © 2015 Springer Science+Business Media New York Source

Zhang Y.,Hubei University of Medicine | Huang J.,National Hospital of Enshi Autonomous Prefecture | Wang P.,Wendeng Central Hospital of Weihai
Medicine (United States)

We aim to build models for peripheral arterial disease (PAD) risk prediction and seek to validate these models in 2 different surveys in the US general population. Model building survey was based on the National Health and Nutrition Examination Surveys (NHANES, 1999-2002). Potential predicting variables included race, gender, age, smoking status, total cholesterol (TC), body mass index, high-density lipoprotein (HDL), ratio of TC to HDL, diabetes status, HbA1c, hypertension status, and pulse pressure. The PAD was diagnosed as ankle brachial index <0.9. We used multiple logistic regression method for the prediction model construction. The final predictive variables were chosen based on the likelihood ratio test. Model internal validation was done by the bootstrap method. The NHANES 2003-2004 survey was used for model external validation. Age, race, sex, pulse pressure, the ratio of TC to HDL, and smoking statuswere selected in the final predictionmodel. The odds ratio (OR) and 95% confidence interval (CI) for age with 10 years increase was 2.00 (1.72, 2.33), whereas that of pulse pressure for 10mm Hg increase was 1.19 (1.10, 1.28). TheOR of PADwas 1.11 (95%CI: 1.02, 1.21) for 1 unit increase in the TC to HDL ratio and was 1.61 (95% CI: 1.40, 1.85) for people who were currently smoking compared with those who were not. The respective area under receiver operating characteristics (AUC) of the final model from the training survey and validation survey were 0.82 (0.82, 0.83) and 0.76 (0.72, 0.79) indicating good model calibrations. Our model, to some extent, has a moderate usefulness for PAD risk prediction in the general US population. © 2016 Wolters Kluwer Health, Inc. All rights reserved. Source

Yu T.,University of Sichuan | Wu Y.,University of Sichuan | Huang Y.,University of Sichuan | Yan C.,University of Sichuan | And 6 more authors.
Molecular Therapy

CXC chemokine receptor 4 (CXCR4) is involved in many human malignant tumors and plays an important role in tumor growth and metastasis. To explore the effects of CXCR4 expression on the malignant cells of oral squamous cell carcinoma (OSCC), Tca8113 and SCC-9 cell lines, as well as their xenograft models, of nude mice were used to detect cancer cell proliferation alteration. This study also examined the corresponding molecular mechanism after CXCR4 knockdown using a recombinant lentiviral vector expressing small interference RNA (siRNA) for CXCR4. RNA interference-mediated knockdown of CXCR4 in highly aggressive (Tca8113 and SCC-9) tumor cells significantly inhibited the proliferation of the two cell lines in vitro and in vivo. The expression levels of >1,500 genes involved in cell cycle, apoptosis, and multiple signaling pathways were also altered. These results provide new evidence of CXCR4 as a promising tumor gene therapeutic target. © The American Society of Gene & Cell Therapy. Source

Wang C.-C.,Weifang Medical College | Zhuang J.,Weifang Traditional Chinese Hospital | Feng F.-B.,Weifang Traditional Chinese Hospital | Wei J.-Y.,Weifang Medical College | And 4 more authors.
Chinese Journal of Cancer Prevention and Treatment

OBJECTIVE: To observe the effect of curcumin on vasculogenic mimicry of human lung cancer A549 cells and expression of Wnt/β-catenin signaling pathway-associated gene, and investigate the mechanism of inhibiting the growth of A549 cells by curcumin. METHODS: A549 cells were cultured with curcumin at various concentrations for 24 hours in vitro and its growth inhibition was detected by MTT method, the transcription levels of β-catenin mRNAs by RT-PCR. Chosen the specific inhibitor-XAV939, then grouped into the Constrast, Curcumin, XAV939 and combined group and observed the expressions of VE-cadherin protein by Western blot. RESULTS:: Curcumin could significantly inhibit the proliferation of the A549 cell line in a dose-dependent manner, and IC50 value on A549 was 17.5 μmol/L. Compared with control group, vascular mimicry formation decreased with dose dependent. Curcumin decreased the intracellular β-catenin gene transcription at mRNA level significantly (all P<0.05). Western blot showed decreased expression of VE-cadherin protein in groups of curcumin compared with the control (P=0.047), no difference was statistically significant among the Curcumin and XAV939(P=0.087). CONCLUSIONS: Curcumin can inhibit the capability of A549 cells on vasculogenic mimicry. It may be caused by the inhibition of Wnt/β- catenin signaling pathway, which will provide new ideas for clinical treatment of lung cancer. ©, 2015, The Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved. Source

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