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Darke C.,Welsh Transplantation and Immunogenetics Laboratory | Corbin S.A.,Welsh Transplantation and Immunogenetics Laboratory
International Journal of Immunogenetics | Year: 2014

Hypersensitivity reactions to the drug abacavir are strongly associated with possession of HLA-B*57:01. Hence, patients with HIV/AIDS who may be prescribed abacavir should be tested for this HLA allele and the drug withheld from those that possess B*57:01. The UK National External Quality Assessment Service for Histocompatibility and Immunogenetics has operated a scheme for B*57:01 testing since 2008 which, in 2013, involved 47 participants from 12 countries. A total of 24 B*57:01-positive, 2 B*57:03-positive and 22 B*57-negative blood samples (including 2 B*58 samples) were distributed to between 28 and 47 laboratories each year over 6 years. Participants, who were unaware of the samples' HLA types, tested and reported on their B*57/B*57:01 status. A total of 1868 reports were assessed over the 6 years. Of the 880 reports on B*57:01 samples, 93.4% were correctly assigned as B*57:01, 2.8% were assigned as groups of B*57 alleles including B*57:01, and 3.3% were reported as B*57 positive only. Over the 6 years, there were four (0.46%) false B*57:01 negative reports. All the B*57:03-positive and B*57-negative samples, involving 72 and 916 assignments, respectively, were essentially reported as B*57:01 negative. Thus, there were no false B57:01 positive assignments. The reporting of B*57:01 status over the last 3 years of the scheme was 99.8% sensitive and 100% specific. Over the last year, it was 100% sensitive and 100% specific. © 2014 John Wiley & Sons Ltd. Source


Darke C.,Welsh Transplantation and Immunogenetics Laboratory | Coates E.,Welsh Transplantation and Immunogenetics Laboratory
Cytometry Part B - Clinical Cytometry | Year: 2010

Background: Flow cytometry-based methods are widely used to detect the ankylosing spondylitis-associated HLA-B27/B2708 antigens. However, the generally used "HLA-B27" monoclonal antibodies (moabs) cross-react with many HLA specificities, including the common HLA-B7 antigen. Thus, using two "B27" moabs is highly recommended. Methods: The assay used two "HLA-B27" reagents, FITC and PE conjugated, respectively and a PE-Cy5 anti-CD3 antibody. Assay verification used 51 reference subjects possessing B*2705, B*2702, and B*2708 and a range of cross-reactive HLA antigens. A total of 1,006 consecutive patients' samples, referred for "HLA-B27 typing", were assayed alongside our standard flow cytometry method. A further 12 low frequency HLA-B*27 specificities were tested. Samples reacting with one "B27" moab only were B*27 allele typed by PCR using sequence-specific primers. Results: All patient B27/B2708 positives (28.3%) were identified by our one-tube method which detected B*2705, B*2702, B*2708, and 8/12 other B*27 specificities. It was unaffected by HLA-B7 and other cross-reactive antigens but required a minor adjustment, a reduction in the volume of one of the "B27" moabs used, to avoid detecting a minority of HLA-B57 subjects. Conclusions: Our one-tube B27/B2708 assay is simple, robust, uses two "B27" moabs for typing precision and security, does not suffer from interference by HLA-B7 or other cross-reactive antigens and has the obvious advantage of using a single tube per typing. © 2009 Clinical Cytometry Society. Source


Bengtsson M.,Uppsala University | Street J.,Welsh Transplantation and Immunogenetics Laboratory | Johnson J.,Welsh Transplantation and Immunogenetics Laboratory | Harvey C.,Welsh Transplantation and Immunogenetics Laboratory | And 2 more authors.
Tissue Antigens | Year: 2014

HLA-A*03:162N differs from A*03:01:01:01 by an exon 4, 664-665insCATG causing E198A and A199X. © 2013 John Wiley & Sons A/S. Source


Street J.,Welsh Transplantation and Immunogenetics Laboratory | Bengtsson M.,Uppsala University | Johnson J.,Welsh Transplantation and Immunogenetics Laboratory | Harvey C.,Welsh Transplantation and Immunogenetics Laboratory | Darke C.,Welsh Transplantation and Immunogenetics Laboratory
Tissue Antigens | Year: 2014

HLA-B*27:05:25 differs from B*27:05:02 by a single synonymous nucleotide substitution (192C>T) at codon 40 in exon 2. © 2014 John Wiley & Sons A/S. Source


Lemin A.J.,Welsh Transplantation and Immunogenetics Laboratory | Darke C.,Welsh Transplantation and Immunogenetics Laboratory
International Journal of Immunogenetics | Year: 2014

Two hundred and fifty-four normal blood donors, from a largely UK European population, were sequence-based typed for HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQA1 and HLA-DQB1. The fit to Hardy-Weinberg expectations was good for all loci (all P values >0.5). Fifteen DQA1 alleles were identified to the second field. DQA1 carriage, allele and DQA1-DQB1 and DRB1-DQA1-DQB1 haplotype frequencies, linkage disequilibria and related values are presented. © 2014 John Wiley & Sons Ltd. Source

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