Weihai Wendeng Center Hospital

Weihai, China

Weihai Wendeng Center Hospital

Weihai, China

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Cong W.,Chinese Academy of Agricultural Sciences | Cong W.,Jilin Agricultural University | Meng Q.-F.,Jilin Entry Exit Inspection and Quarantine Bureau | Li B.,Wendeng Peoples Hospital | And 6 more authors.
International Journal of Infectious Diseases | Year: 2014

Objective: Hepatitis E, caused by the hepatitis E virus (HEV), is an important global public health concern. There are conflicting reports in regard to the association of HEV infection and psychiatric disorders. Therefore, we investigated this association in a Chinese population through a case-control seroprevalence study. Methods: A case-control study of 445 psychiatric patients and 445 control subjects was conducted between June 2011 and July 2013 to estimate the seroprevalence and potential risk factors associated with acquiring HEV infection in psychiatric patients in China. Demographic and clinical data and blood samples were collected, and anti-HEV IgG and IgM antibodies were measured by ELISA method. Results: The prevalence of anti-HEV IgG in psychiatric patients (27.19%) was higher than that in healthy controls (19.55%) (p= 0.07).In addition, there were significant differences in seroprevalences of HEV infection according to the type of psychiatric disorder. Sex, age, contact with dogs and cats, and exposure to soil were found to be risk factors for HEV infection. Conclusions: Psychiatric patients have a higher prevalence of HEV infection than control subjects in China. Considering the promotion of public health, psychiatric patients should be considered a specific group of HEV infection. © 2014 The Authors.


Wang F.,Qingdao University | Lu K.,Qingdao University | Zhang S.,Weihai Wendeng Center Hospital | Liu S.,Shandong Provincial Key Laboratory of Metabolic Disease | And 2 more authors.
Clinical Endocrinology | Year: 2014

Objective The aim of this study was to screen for DUOX2, TPO and TG mutations in Chinese patients with congenital hypothyroidism (CH) and goitre and to define the relationships between DUOX2 genotypes and clinical phenotypes. Methods Blood samples were collected from 67 patients with CH and goitre in Shandong Province, China. Genomic DNA was extracted from peripheral blood leucocytes. PCR and direct sequencing were used to analyse all exons of DUOX2, TPO and TG. Detailed medical records were then collected, and the relationship between DUOX2 genotype and the clinical phenotype of CH and goitre caused by DUOX2 mutations was investigated. Results Analysis of DUOX2 revealed nine mutations, including one novel nonsense mutation (p.W734X), six novel missense mutations (p.N100D, p.S660L, p.A1131S, p.W1181G, p.A1206T and p.R1267W) and two recurrent mutations (p.R701X and p.R1110Q) in 10 patients from 10 unrelated families. Monoallelic and compound heterozygous mutations in DUOX2 were associated with permanent or transient CH. No mutation was found after screening all exons of TPO and TG. Conclusion Our study identified DUOX2 mutations in 14·9% of Chinese patients investigated with CH and goitre. Because the relationships between DUOX2 genotypes and clinical phenotypes are extremely complex, however, further studies are needed to identify more mutations in known genes which are involved in CH and goitre. © 2014 John Wiley & Sons Ltd.


PubMed | The 404th Hospital of PLA, Centers for Disease Control and Prevention, Shandong Academy of Occupational Health and Occupational Medicine, Weihai Wendeng Center Hospital and 2 more.
Type: | Journal: Chemosphere | Year: 2016

As a widely used herbicide, atrazine (AZ) has been extensively studied for its adverse effects on the reproductive system, especially feminization in male animals. However, the relationship of gene expression changes and associated toxicological endpoints remains unclear. In this study, developing Xenopus laevis tadpoles were exposed to concentration of AZ at 0.1, 1, 10 or 100g/L continuously. Compared with froglets in the control group, there were no significant differences in body length, body weight, liver weight and hepatosomatic index (HSI) of males in groups treated with AZ for 90d. At 100g/L AZ treatment caused a significant reduction of gonad weight and gonadosomatic index (GSI) of males (p<0.01). In addition, AZ at all dose levels caused testicular degeneration, especially in froglets from the groups with 0.1 and 100g/L which exhibited U-shaped dose-response trend. We further investigated the gene expression changes associated with the testicular degeneration induced by AZ. We found that the expression of 1165 genes was significantly altered with 616 upregulated and 549 downregulated compared to the expression profile of the control animals. KEGG analysis showed that genes which were significantly affected by AZ are mainly involved in arginine and proline metabolism, cell cycle, riboflavin metabolism, spliceosome, base excision repair and progesterone-mediated oocyte maturation pathway. Our results show that AZ may affect reproductive and immune systems by interference with the related gene expression changes during the male X.laevis development. The findings may help to clarify the feminization mechanisms of AZ in male X.laevis.


Sai L.,Shandong Academy of Occupational Health and Occupational Medicine | Dong Z.,The 404th Hospital of PLA | Li L.,Shandong Academy of Occupational Health and Occupational Medicine | Guo Q.,Shandong Academy of Occupational Health and Occupational Medicine | And 10 more authors.
Chemosphere | Year: 2016

As a widely used herbicide, atrazine (AZ) has been extensively studied for its adverse effects on the reproductive system, especially feminization in male animals. However, the relationship of gene expression changes and associated toxicological endpoints remains unclear. In this study, developing Xenopus laevis tadpoles were exposed to concentration of AZ at 0.1, 1, 10 or 100 μg/L continuously. Compared with froglets in the control group, there were no significant differences in body length, body weight, liver weight and hepatosomatic index (HSI) of males in groups treated with AZ for 90 d. At 100 μg/L AZ treatment caused a significant reduction of gonad weight and gonadosomatic index (GSI) of males (p < 0.01). In addition, AZ at all dose levels caused testicular degeneration, especially in froglets from the groups with 0.1 and 100 μg/L which exhibited U-shaped dose-response trend. We further investigated the gene expression changes associated with the testicular degeneration induced by AZ. We found that the expression of 1165 genes was significantly altered with 616 upregulated and 549 downregulated compared to the expression profile of the control animals. KEGG analysis showed that genes which were significantly affected by AZ are mainly involved in arginine and proline metabolism, cell cycle, riboflavin metabolism, spliceosome, base excision repair and progesterone-mediated oocyte maturation pathway. Our results show that AZ may affect reproductive and immune systems by interference with the related gene expression changes during the male X. laevis development. The findings may help to clarify the feminization mechanisms of AZ in male X. laevis. © 2016 Elsevier Ltd.


Liu S.G.,Qingdao University | Zhang S.S.,Weihai Wendeng Center Hospital | Zhang L.Q.,Qingdao University | Li W.J.,Qingdao University | And 7 more authors.
Journal of Endocrinological Investigation | Year: 2012

Background: Congenital hypothyroidism (CH) is a neonatal endocrine disease with an incidence of 1:2000 to 1:4000 worldwide. In about 85% of patients CH is secondary to thyroid dysgenesis, but its pathogenesis remains unclear. Thyroid transcription factors, such as paired box transcription factor 8 ( PAX8), play an important role in thyroid organogenesis and development. Aim: To screen PAX8 mutations in Chinese CH patients and characterize the features of PAX8 mutations in China. Materials and methods: Blood samples were collected from 300 CH patients in Shandong Province, China, and genomic DNA was extracted from peripheral blood leukocytes. Using PCR and direct sequencing, exon 3 and exon 4 of PAX8 were analyzed. Results: Analysis of PAX8 in 300 CH patients revealed heterozygous missense mutations or variations in two unrelated patients; one was a known missense mutation G92A, resulting in an arginine to histidine substitution at codon 31, the other was a missense variation G122T, resulting in the substitution of a glycine at position 41 by a valine residue. The patient with the R31H mutation had CH with thyroid hypoplasia, while the patient with the G41V variation had CH with a eutopic and normal-sized thyroid gland. Conclusion: We report a heterozygous missense mutation and a variation in PAX8 in two out of 300 unrelated Chinese CH patients, showing that the PAX8 mutation rate is very low in CH patients in China. ©2012, Editrice Kurtis.


Wang J.,Qingdao University | Ma X.,Qingdao University | Qu S.,Weihai Wendeng Center Hospital | Li Y.,Qingdao University | And 5 more authors.
Experimental and Clinical Cardiology | Year: 2013

OBJECTIVES: To investigate the prevalence of subclinical thyroid dysfunction and the relationship between thyrotropin levels and cardiovascular risk factors in residents of the coastal area of China. METHODS: A total of 4256 individuals (mean [± SD] age 50.51±14.24 years; 2079 males, 2177 females,) were enrolled in the present study. Sex, blood pressure, body mass index, waist-to-hip ratio, serum levels of fasting glucose, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, uric acid and smoking status were measured. The relationship between thyrotropin levels and cardiovascular risk factors was analyzed. RESULTS: The overall prevalence of thyroid dysfunction was 11.07%. The prevalence of subclinical hypothyroidism (6.32%) was higher than that of hyperthyroidism (1.53%). The prevalence of thyroid dysfunction among female subjects was higher than that among male subjects (16.54% versus 5.34%, respectively; P<0.001). Significant differences were detected with respect to body mass index (P=0.026), waist-to-hip ratio (P<0.001), fasting glucose levels (P=0.001), total cholesterol levels (P=0.013), triglyceride levels (P=0.003) and smoking status according to different thyrotropin levels. CONCLUSION: The prevalence of thyroid dysfunction was high in residents of China's coastal area. Significant differences were detected with regard to body mass index, waist-to-hip ratio, fasting glucose levels, total cholesterol levels, triglyceride levels and smoking status according to different thyrotropin levels. ©2013 Pulsus Group Inc. All rights reserved.


Wang M.,Weihai Wendeng Center Hospital | Bie Z.,Weihai Wendeng Center Hospital | Guo L.,Yan'an University | Wang J.,Ningxia Medical University
Experimental and Clinical Cardiology | Year: 2014

Objective: H-type hypertensive patients suffer from high blood pressure and high level of plasma homocysteine (Hcy), at great risk for cardiovascular disease (CVD). We aim to evaluate the effect of a compound tablet (enalapril folic acid tablet) on reducing blood pressure, plasma Hcy level, as well as heart function/structure. Methods: 200 patients were included in a randomized, double-blinded study. Group A was treated with enalapril only and group B was treated with enalapril folic acid. Blood pressure, plasma Hcy level, heart rate variability and left ventricular hypertrophy were measured and analyzed at the beginning of the study, and 6-month, 1-year, and 3-year after treatment. Results: Since 6-month after treatment, enalapril folic acid significantly reduced blood pressure, plasma Hcy level, and heart function/structure. Though enalapril alone was able to control blood pressure effectively, enalapril folic acid significantly reduced the risk for the onset of angina as well. Conclusions: Enalapril folic acid tablet is an effective treatment for H-type hypertension. It is able to control blood pressure and lower plasma Hcy; more importantly, it can improve ventricular structure and function and reduce the risk of angina.


Sai L.,Shandong Academy of Occupational Health and Occupational Medicine | Qu B.,China Shandong Medical College | Li Y.,Shandong Provincial Chest Hospital | Jia Q.,Shandong Academy of Occupational Health and Occupational Medicine | And 7 more authors.
Bulletin of Environmental Contamination and Toxicology | Year: 2016

This study continued our previous work (Sai et al. in Bull Environ Contam Toxicol 95:157–163, 2015a) by analysing the effects of simazine on the liver histological structure and metamorphosis in the developing Xenopuslaevis. Tadpoles (Nieuwkoop-Faber stage 46) were exposed to simazine at 0.1, 1.2, 11.0 and 100.9 μg/L for 100 days. When tadpoles were exposed to simazine at 11.0 and 100.9 µg/L, an increased mortality and damaged liver tissues were observed together with significant inhibition of percent of X. laevis completing metamorphosis on days 80 and 90 and prolonged time of completing metamorphosis. On the other hand, we found that simazine has no significant effects on liver weight and altered hepatosomatic index. Results of this study may be considered to inform risk assessment of the effects of simazine on the development of X. laevis. © 2016 Springer Science+Business Media New York


PubMed | University of Queensland, Shandong Provincial Chest Hospital, Weihai Wendeng Center Hospital, China Shandong Medical College and Shandong Academy of Occupational Health and Occupational Medicine
Type: Journal Article | Journal: Bulletin of environmental contamination and toxicology | Year: 2016

This study continued our previous work (Sai et al. in Bull Environ Contam Toxicol 95:157-163, 2015a) by analysing the effects of simazine on the liver histological structure and metamorphosis in the developing Xenopus laevis. Tadpoles (Nieuwkoop-Faber stage 46) were exposed to simazine at 0.1, 1.2, 11.0 and 100.9g/L for 100days. When tadpoles were exposed to simazine at 11.0 and 100.9g/L, an increased mortality and damaged liver tissues were observed together with significant inhibition of percent of X. laevis completing metamorphosis on days 80 and 90 and prolonged time of completing metamorphosis. On the other hand, we found that simazine has no significant effects on liver weight and altered hepatosomatic index. Results of this study may be considered to inform risk assessment of the effects of simazine on the development of X. laevis.


He R.,Ningxia Medical University | Bie Z.,Weihai Wendeng Center Hospital | Wang J.,Ningxia Medical University | Mu B.,Ningxia Medical University
Experimental and Clinical Cardiology | Year: 2014

Background: Heart failure is a chronic condition leading to high morbidity and mortality. Clinical diagnosis of heart failure at early stages is important for patients to get immediate treatment to control the condition. Our goal is to develop a statistical model based on a few easily measurable health variables to provide valuable information for clinical diagnosis of heart failure. Methods: 248 patients with suspected symptoms of heart failure were clinically evaluated. A range of variables potentially associated with heart failure were measured for each patient. A multivariate logistic regression model was established based on the clinical evaluation data of these patients and their diagnosis. Results: multivariate logistic regression demonstrated that five variables are significantly contributing to heart failure in our model, including heart rate [odds ratio (OR) 0.90, 95% Confidence interval (CI) 0.84-0.97], ejection fraction (OR 10.32, CI 3.02-35.2), high cholesterol level (OR 6.05, CI 2.08-17.57), myocardial infarction (OR 0.223, CI 0.06-0.84) and lung disease (OR 12.34, CI 1.74-193.37). The middle 50% of each group of patients are predicted to distribute separately on the box plot. By using a 0.672 cutoff value on the predicted probability, our model is able to predict heart failure with 85% accuracy. Conclusions: our novel binary logistic regression model is able to predict the probability of heart failure in patients with 15 easily measurable health parameters with 85% accuracy. Such information can help doctors make quick and accurate clinical diagnosis for elderly patients with great risks for heart failure.

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