Jiang Y.-Y.,Weifang Medical University
Oral Health and Preventive Dentistry | Year: 2017
Purpose: To assess the prevalence of early childhood caries (ECC) in 2- to 5-year-old preschool children in Weifang City, China, and to investigate the factors related to this condition. Materials and Methods: A cross-sectional study was carried out among 2829 children (2-5 years old, including 1601 boys and 1228 girls) in twenty kindergartens in Weifang City, China, in 2014. These children were selected via cluster sampling. First, the percentages of ECC and severe ECC (S-ECC) as well as the mean dmft were compared between different genders and ages, and the tooth distribution pattern of ECC was analysed. Meanwhile, the information regarding feeding and oral hygiene practices was collected by a questionnaire with 14 questions to evaluate their relationships with the percentages of ECC and S-ECC as well as the mean dmft. The chi-squared test, t-test and one-way ANOVA were conducted to evaluate the significant differences. Results: The ECC prevalence of the preschoolers was 53.3%, and the mean dmft was 2.12 ± 2.56. 71.4% of the 1509 children with ECC were identified as S-ECC. Not only the percentages of ECC and S-ECC but also the mean dmft differed statistically significantly according to gender and age (p < 0.05). Furthermore, caries was most common in primary maxillary central incisors. Feeding and oral hygiene practices were shown to be the significant determining factors for caries. Conclusions: The oral health status of children living in Weifang is very poor. Appropriate strategies must be implemented to promote oral health early on by improving feeding and oral hygiene practices. © Quintessenz. All right reserved.
Sheng J.W.,Weifang Medical University
Journal of Chemical and Pharmaceutical Research | Year: 2014
Polysaccharides extracted from Athyrium multidentatum (Doll.) Ching rhizome (AMCP) were chemically modified by introducing different functional groups. The acetylated, sulphated and phosphorylated derivatives were synthesized successfully, and characterized by IR spectra. The antioxidant activities of polysaccharide derivatives were explored employing various established in vitro systems. Acetylated derivative was prepared using N-bromosuccinimide (NBS) as catalyst. Polyphosphoric acid method was applied in phosphorylation reaction. The phosphorylated derivative exhibited the greatest antioxidant activity among all derivatives, and possessed much stronger reducing power than AMCP. Available data suggested that the substituted groups of AMCP played important roles on the antioxidant activity, and the mechanism on the antioxidant activity of acetyl, sulphonyl and phosphoryl group was indicated. © 2014, Journal of Chemical and Pharmaceutical Research. All rights reserved.
Qi H.,Weifang Medical University |
Sheng J.,Weifang Medical University
Marine Drugs | Year: 2015
Numerous studies have suggested that hyperlipidemia is closely linked to cardiovascular disease. The aim of this study was to investigate the possible antihyperlipidemia mechanism of HU (high sulfate content of ulvan) in high-cholesterol fed rats. Wistar rats were made hyperlipidemic by feeding with a high-cholesterol diet. HU was administered to these hyperlipidemia rats for 30 days. Lipid levels and the mRNA expressions of FXR, LXR and PPARγ in liver were measured after 30 days of treatment. In the HU-treated groups, the middle dosage group of male rats (total cholesterol (TC): p < 0.01) and the low-dosage group of female rats (TC, LDL-C: p < 0.01) showed stronger activity with respect to antihyperlipidemia. Moreover, some HU groups could upregulate the mRNA expression of FXR and PPARγ and downregulate the expression of LXR. For the male rats, compared with the hyperlipidemia group, the middle dosage HU had the most pronounced effect on increasing the mRNA levels of FXR (p < 0.01); low- and high-dosage HU showed a significant inhibition of the mRNA levels of LXR (p < 0.01). All HU female groups could upregulate the mRNA expression of PPARγ in a concentration-dependent manner. In summary, HU could improve lipid profiles through upregulation of FXR and PPARγ and downregulation of LXR. © 2015 by the authors; licensee MDPI.
Wei X.-H.,Weifang Medical University |
Ji L.-L.,Weifang Medical University
Neuroscience Letters | Year: 2014
The aim was to examine the effect of handball training on cognitive ability in elderly with mild cognitive impairment. A total of 60 elderly were randomly divided into training group (n= 30) and control group (n= 30). The mini-mental state examination (MMSE) score and abilities of daily living scale (ADL) score before, after 3-month, and after 6-month intervention period was measured. The results showed that MMSE score was increased and ADL score was decreased in training group after 3-month and 6-month intervention (P<. 0.05), while there were no significant changes in MMSE or ADL in control group (P£3/40.05). These preliminary results indicated that handball training can improve cognitive ability in elderly with MCI. © 2014 Elsevier Ireland Ltd.
Duan Y.,Weifang Medical University
Journal of Chemical and Pharmaceutical Research | Year: 2014
The structure changes of flavonoids in strong acid and alkaline solution can be characterized by means of ultraviolet-visible (UV-Vis) spectrum. In this paper, the shifts of UV-Vis spectrum of quercetin in aqueous ethanol solution with different pH values were investigated. The UV-Vis spectrum of quercetin in 50% (v/v) aqueous ethanol solution showed two characteristic adsorption bands setting at 374 nm and 256 nm, respectively. When the pH value was adjusted to 14.0 with sodium hydroxide, the band at 374 nm shifted to about 400 nm, and the band at 256 nm disappeared, a new band at 314 nm appeared. The pH value of alkaline solution was adjusted to 7.0 with hydrochloric acid, and the UV-Vis spectrum of precipitate was the same with quercetin, but the UV-Vis spectrum of filtrate was different. When the pH value of quercetin in 50% (v/v) aqueous ethanol solution was adjusted to 1.0 with hydrochloric acid, there was little shift of UV-Vis spectrum. But after concentrating the acid solution and filtering, the UV-Vis spectrum of precipitation was the same with quercetin, while the UV-Vis spectrum of filtrate was different. It was concluded that strong acid and alkaline caused some degradation of quercetin. © 2014, Journal of Chemical and Pharmaceutical Research. All Rights Reserved.
Wei L.,Weifang Medical University |
Wei L.,St. John's University |
Lu J.,St. John's University |
Xu H.,St. John's University |
And 3 more authors.
Drug Discovery Today | Year: 2015
Silver nanoparticles (AgNPs) have been widely used in biomedical fields because of their intrinsic therapeutic properties. Here, we introduce methods of synthesizing AgNPs and discuss their physicochemical, localized surface plasmon resonance (LSPR) and toxicity properties. We also review the impact of AgNPs on human health and the environment along with the underlying mechanisms. More importantly, we highlight the newly emerging applications of AgNPs as antiviral agents, photosensitizers and/or radiosensitizers, and anticancer therapeutic agents in the treatment of leukemia, breast cancer, hepatocellular carcinoma, lung cancer, and skin and/or oral carcinoma. © 2014 Elsevier Ltd. All rights reserved.
Lin W.,Weifang Medical University |
Tongyi S.,Weifang Medical University
Tumor Biology | Year: 2014
Green tea polyphenol (GTP) is one of the most promising chemopreventive agent for cancer; it can inhibit cancer cell proliferation and induce apoptosis through p53-dependent cell signaling pathways. Unfortunately, many tumor cells lack the functional p53, and little is known about the effect of GTP on the p53-deficient/mutant cancer cells. To understand the p53-independent mechanisms in GTP-treated p53-dificient/mutant cancer cells, we have now examined GTP-induced cytotoxicity in human hepatoma Hep3B cells (p53-deficient). The results showed that GTP could induce Bax and Bak activation, cytochrome c release, caspase activation, and necroptosis of Hep3B cells. Bax and Bak, two key molecules of mitochondrial permeability transition pore (MPTP), were interdependently activated by GTP, with translocation and homo-oligomerization on the mitochondria. Bax and Bak induce cytochrome c release. Importantly, cytochrome c release and necroptosis were diminished in Hep3B cells (Bax−/−) and Hep3B cells (Bak−/−). Furthermore, overexpression of Bcl-2 could ameliorate GTP-induced cytochrome c release and necroptosis. Together, the findings suggested that GTP-induced necroptosis was modulated by the p53-independent pathway, which was related to the translocation of Bax and Bak to mitochondria, release of cytochrome c, and activation of caspases. © 2014, International Society of Oncology and BioMarkers (ISOBM).
Niu N.,Weifang Medical University
Investigative ophthalmology & visual science | Year: 2013
RPE is a key component of the blood-ocular barrier (BOB) and is equipped with immunological molecules such as toll-like receptors (TLRs) and complement receptors, which together orchestrate the innate and adaptive immunity of the eye. Immunoglobulin G (IgG) in the aqueous humor and vitreous body has traditionally been thought to be derived from serum via transcytosis across the BOB. Our previous work validated production of endogenous IgG by RPE cells locally. However, the function and role of this IgG in the intraocular immunity is poorly understood. After confirming IgG production in a human RPE cell line (ARPE-19) with immunofluorescence, in situ hybridization, and RT-PCR, we further investigated the function of endogenous IgG in RPE biology with MTS, flow cytometry, and cell invasion analysis after downregulation of IgG by siRNA. Involvement of the TLR4 pathway was also studied using Western blot, ELISA and confocal microscopy. Endogenous IgG is crucial for support of proliferation, mitosis, migration, and inhibition of apoptosis of RPE. Moreover, production of endogenous IgG by RPE is regulated by the TLR4 pathway in a concentration- and duration-dependent manner, and IgG affects the activation of the TLR4 pathway in a synergistic manner. Activation of the FcγR I pathway and production of IL-10 could be induced by IgG derived from RPE. These data suggest that endogenous IgG may be a molecule that is essential for the physiological function of RPE, and suggest IgG is important for regulating intraocular immune responses under physiologic and pathologic conditions.
Sheng J.,Weifang Medical University |
Sun Y.,Weifang Medical University
Carbohydrate Polymers | Year: 2014
Different molecular weight polysaccharides were prepared by degradation of polysaccharides extracted from Athyrium multidentatum (Doll.) Ching rhizome (CPA) with hydrogen peroxide and ascorbic acid. Four low molecular polysaccharides derivatives (CPA-1, CPA-2, CPA-3 and CPA-4) were successfully obtained and had their antioxidant activities investigated employing various established in vitro systems. All CPA derivatives showed pronounced antioxidant activity, and had stronger antioxidant ability than CPA in certain tests. CPA-1 exhibited the strongest scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical among all samples, and the IC50 value was 25 μg/mL. CPA-2 possessed the highest scavenging ability against superoxide radical at 200 μg/mL. The scavenging activity of CPA-4 on hydroxyl radical was higher than CPA from 120 to 200 μg/mL. The mechanism on influence the antioxidant activity of CPA and its degraded derivatives was indicated. © 2014 Elsevier Ltd.
Liu X.-L.,Weifang Medical University |
Zhang W.,Weifang Medical University |
Tang S.-J.,Weifang Medical University
International Journal of Clinical and Experimental Pathology | Year: 2014
Adipose tissue-derived mesenchymal stem cells (ADSCs) are of great interest as a cellular therapeutic agent for regenerative and immunomodulatory purposes. The aim of this study was to investigate whether ADSCs transplantation could promote nerve repair in rats of cerebral ischemia-reperfusion (I/R) injury. We isolated and cultured human ADSCs, and then measured cell surface antigens by flow cytometry and immunofluorescence. Healthy SD rats were randomly divided into sham group, MCAO group, MCAO+vehicle group and MCAO+ADSCs group. Cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion (MCAO). Then the human ADSCs were transplanted into the brain of rats 24 h after MCAO. The mRNA level of BDNF (brain derived neurotrophic factor, BDNF), NGF (nerve growth factor, NGF) and bFGF (basic fibroblasts growth factor, bFGF) were detected by real-time PCR at different time points (d7, d14, d21 and d28 after MCAO). Meanwhile, the neurological deficit scores were estimated. The neurological deficit of rats in MCAO+ADSCs group attenuated at d7 in contrast to the MCAO+vehicle group (P<0.05). Subsequently, they were dramatically ameliorated with the time especially at d28. At d7, d14, d21 and d28 after ADSCs transplantation, BDNF, NGF and bFGF mRNA in MCAO+ADSCs group were strikingly higher than those in MCAO+vehicle group, and these two groups both reached the peak at d14. The western blotting results showed that BDNF and Bcl-2 expressed higher in MCAO+ADSCs group than MCAO+vehicle group. Therefore, our current results suggest that ADSCs promote nerve repair after injury through elevating the expression of neurotrophic factors and inhibiting the apoptosis of neural cells.