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Weifang, China

Chen J.-L.,Shandong Academy of Sciences | Chen F.,Wei Fang Peoples Hospital | Zhang T.-T.,Shandong University | Liu N.-F.,Shandong University
International Journal of Molecular Medicine

Epithelial ovarian cancer (EOC), the sixth most common cancer in women worldwide, is the most commonly fatal gynecologic malignancy in developed countries. One of the main reasons for this is that relatively little was known about the molecular events responsible for the development of this highly aggressive disease. In the present study, we demonstrated that salt inducible kinase 1 (SIK1; which is also known as MSK/SIK/SNF1LK) was downregulated in ovarian cancer tissue samples. Using HEY ovarian cancer cells, we noted that SIK1 overexpression inhibited proliferation as well as cancer stem cell-associated traits. Silencing SIK1 promoted the proliferation of the EG ovarian cancer cell line. We performed an analysis of potential microRNAs (miRNAs or miRs) target sites using three commonly used prediction algorithms: miRanda, TargetScan and PicTar. All three algorithms predicted that miR-141 targets the 3'UTR of SIK1. Subsequent experiments not only confirmed this prediction, but also showed that miR-141 was associated with the progression of this disease. Finally, we found that miR-141 promoted proliferation of EG cells, whereas silencing miR-141 restored SIK1 expression and inhibited the proliferation of the HEY cells. Elucidating the molecular mechanism of ovarian cancer not only enables us to further understand the pathogenesis and progression of the disease, but also provides new targets for effective therapies. Source

Wang F.,Qingdao University | Wang F.,Weifang Medical University | Chen F.,The City Government Organ Hospital | Li H.-L.,Wei Fang Peoples Hospital | And 4 more authors.
Biomedical Research (India)

The aim of this study was to report a case that underwent a craniotomy after primary pre-operative cerebrovascular and 3D digital subtraction angiography (DSA) showed false results. DSA misdiagnosis and missed diagnosis of aneurysms were found, and the subject was subsequently treated accordingly. This case was analysed to identify: 1) the cause of the false positive and false negative results in DSA, 2) the limitations of 3D DSA and 3) the experience acquired and lessons learned. A 54- year-old spontaneous subarachnoid haemorrhage patient underwent DSA and 3D DSA under local anaesthesia. Results revealed aneurysm at the anterior cerebral A1 end. Considering the excessively large tumour angle circuitry, we decided to adopt craniotomy and clipping under direct vision. Misdiagnosis and missed diagnosis were intra-operatively noticed, and the aneurysm was clipped out. About half a month after the operation, the patient underwent DSA again under full coordination. The results from these two DSAs were compared. An originally suspected aneurysm was merely a pseudo-anterior communicating artery; the real aneurysm was located in the starting zone of A1. Active cooperation (or anaesthesia) made 3D DSA clear and complete. Moreover, the two 3D DSAs were both ineffective in identifying false positive aneurysms because of anatomical variation. 3D DSA is still limited in differentiating or developing anatomical variations of cerebrovascular vessels, thereby causing false positive results. The use of 3D DSA requires full cooperation from the patient. The guiding effect of the clot accumulation area on aneurysms should be considered during operation. © 2015, Scientific Publishers of India. All rights reserved. Source

Wang M.,Weifang Center for Maternal | Zhang B.,Wei Fang Peoples Hospital | Dai X.,Weifang Center for Maternal | Zhang Y.,Weifang Center for Maternal | Lian W.,Weifang Center for Maternal
International Journal of Clinical and Experimental Pathology

Background: MicroRNA-433 (miR-433), possessing tumor suppressive activity, has been found to be down-regulated in different types of cancer. However, its clinical significance in epithelial ovarian cancer (EOC) is still unclear. Therefore, the aim of this study was to detect the miR-433 expression and its prognostic value in patients suffering from EOC. Methods: The miR-433 expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR) analysis in 115 EOC tissues and 45 normal tissues. Then, the associations of miR-433 expression with clinicopathologic characteristics as well as overall survival of EOC patients were determined by Chi-square test and Kaplan-Meier method respectively. Besides, the prognostic value of miR-433 was estimated via Cox regression analysis. Results: The expression of miR-433 in EOC tissues were significantly lower than that in normal tissues (P<0.05). In addition, low miR-433 expression was found to be closely correlated with tumor size (P=0.050), advanced FIGO stage (P=0.009), and recurrence (P=0.002). Moreover, the Kaplan-Meier analysis demonstrated that EOC patients with low miR-433 expression had a poorer overall survival than those with high miR-433 expression (P=0.000). Furthermore, the multivariate analysis identified miR-433 (P=0.013; HR=2.973; 95% CI=1.260-7.012) was an independent prognostic factor for EOC patients. Conclusion: For the first time, the current study offered convincing evidence that the expression of miR-433 was decreased in EOC and it might be associated with tumor progression of EOC. Therefore, miR-433 may be an independent prognostic marker for EOC patients. Source

Liu F.-X.,Wei Fang Peoples Hospital | Li Y.-X.,Wei Fang Peoples Hospital | Zhang X.-D.,Wei Fang Peoples Hospital | Ren C.-A.,Wei Fang Peoples Hospital | And 2 more authors.
Chinese Medical Journal

Background Multiple epiphysis dysplasia (MED) is a common skeletal dysplasia with a significant locus heterogeneity. In the majority of clinically defined cases, mutations have been identified in the gene encoding cartilage algometric matrix protein (COMP). Methods Five patients were included in the study. Linkage analysis and mutation analysis of the COMP gene were conducted in the patients and their family members. Results We have identified a novel mutation in axon 14 of COMP gene in the family. Conclusions This mutation produced a severe MED phenotype with marked short stature, early onset osteoarthritis, and remarkable radiographic changes. Our results extended the range of disease-causing mutations in COMP gene and contributed more information about relationship between mutations and phenotype. Source

Zhang Y.,Wei Fang Peoples Hospital | Feng Y.,Wei Fang Peoples Hospital | Cao B.,Wei Fang Peoples Hospital | Cao B.,Qingdao University | Tian Q.,Wei Fang Peoples Hospital
Archives of Medical Science

Introduction: The aim of the study was to investigate the significance of factors associated with minimal hepatic encephalopathy (MHE) in cirrhotic patients. Material and methods: Between September 2012 and August 2013, 60 cirrhotic patients, including MHE and non-MHE (NMHE) patients, were included in the study. Associated factors and clinical factors were analyzed to see if they were significantly different between MHE and non-MHE patients. Upon identifying the factors showing differences, we applied multivariate regression analysis to further decide which were the most significant ones to differentiate MHE from NMHE patients. Results: There were 26 patients diagnosed with MHE and 34 with NMHE. Our results demonstrated that the prevalence rate of small intestinal bacterial overgrowth (SIBO) was highly associated with patients with MHE (65.4%, 17 out of 26), in contrast to the rate in NMHE patients (8.8%, 3 out of 34). We also found that factors including age, education level, intelligent test results, plasma albumin level and plasma ammonia levels were significantly different between MHE and NMHE patients. Ultimately, with logistic regression analysis, we found that SIBO was the most significant factor differentiating MHE patients from NMHE patients (p < 0.05). Conclusions: In cirrhotic patients, SIBO was highly associated with MHE. This may further our understanding of the mechanisms of MHE and help to develop potential therapeutic interventions to treat cirrhotic patients with MHE. Copyright © 2015 Termedia & Banach. Source

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