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Linge K.,Water Quality Research Center | Blair P.,Water Corporation of Western Australia | Busetti F.,Water Quality Research Center | Handyside M.,Water Corporation of Western Australia | And 7 more authors.
Water | Year: 2010

The Western Australia's Premier's Collaborative Research Program (PCRP) project 'Characterising Treated Wastewater for Drinking Purposes Following Reverse Osmosis Treatment' commenced in October 2005, to determine the potential risks of replenishing drinking water aquifers with MF/RO treated secondary wastewater from Perth's wastewater treatment plants, A brief report on the project won the Michael Flynn Award for the best posterpaper at Ozwater'10. The results included those published in Water, February 2010, by Rodriguez et al, entitled Efficiency of RO for Removal of Chemical Contaminants. Consequently, thisversion has been drafted to cover the other aspects of the study, principally the identification of suitable indicators which could be used to validate treatment performance.


Lee Y.-J.,Water Quality Research Center | Park J.-G.,Daegu University | Rhee O.-J.,Dankook University | Lee G.-C.,Water Quality Research Center
Journal of Pure and Applied Microbiology | Year: 2014

On the basis of various guidelines for drinking-water quality, five species of bacteria (Helicobacter pylori, Salmonella typhimurium, Yersinia enterocolitica, Legionella pneumophila, and Campylobacter jejuni] were selected as representatives of waterborne microbial pathogens. Matrix-associated laser-desorption/ionization timeof-flight mass spectrometry (MALDI-TOF MS) analysis was conducted to identify the five bacteria, and the MALDI-TOF MS results were compared with results obtained through 16S rRNA identification. The MALDI-TOF MS analysis yielded score values of 1.60 ± 0.19, 2.41 ± 0.09, 2.37 ± 0.16, 2.19 ± 0.15, and 1.85 ± 0.08 for H. pylori, S. typhimurium, Y. enterocolitica, L. pneumophila, and C. jejuni, respectively. In addition, the MALDI-TOF identification results were well-correlated with those from the 16S rRNA identification. This study suggests that MALDI-TOF-based identification is an inexpensive, rapid, reproducible, and accurate alternative method for the identification of waterborne microbial pathogens in aquatic environments.


Lee G.-C.,Water Quality Research Center | Kim M.-J.,Water Quality Research Center | Kim J.I.,Chungbuk National University | Lee C.H.,Chungbuk National University
Journal of Microbiology and Biotechnology | Year: 2014

The occurrence of human norovirus (NoV) genogroup I (GI) and genogroup II (GII) strains was investigated in Korea. Between 2007 and 2010, 265 samples were collected from 89 Korean water source locations. NoV GI was detected in 4.5% and NoV GII in 1.5%. Samples collected in winter had the highest occurrence; 9.4% for NoV GI and 6.3% for NoV GII. NoV GI detection was highest in groundwater, with the next highest in river water and the lowest in lake water (5.9%, 5.4%, and 1.6%, respectively), and NoV GII was found only in river water. When three representative Korean basin systems (Han (H)-, Geum/Seom (G/S)-, and Nakdong (N)-river basins) were compared, both NoV genogroups were high in the G/S-, but absent in the H- river basin. The most prevalent genotypes within the GI and GII groups were GI.5 and GII.4, respectively. The NoVs found in surface water were identical to those found in patients and those found in groundwater. The NoVs appeared to be transmitted from the patient to the surface water, and then to the groundwater, suggesting a fecal-oral route of transmission. This is the first nationwide surveillance of NoV in major Korean water sources. © 2014 by The Korean Society for Microbiology and Biotechnology.


Lee Y.-J.,Water Quality Research Center | Kim B.-S.,Chunlab Inc. | Kim M.-J.,Water Quality Research Center | Nam S.,Water Quality Research Center | And 5 more authors.
Journal of Pure and Applied Microbiology | Year: 2014

The occurrence of Actinomycetes, their molecular characteristics, and their release of synthesized odor compounds into soil and the water systems were investigated. The average numbers of Actinomycetes was 9.0 × 105 CFU/g in the soil and 4.8 CFU/mL in the water. The average numbers of Actinomycetes in the soil was highest at the Yangpyeong (YP) testing site (2.2 × 106 CFU/g), followed by the Gyeongan (GA), Paldang (PD), and Cheongpyeong (CP) sites. The Streptomyces genus, which can produce odor compounds such as geosmin and 2-Methylisoborneol (MIB), was dominant (67.6%). An enhanced proportion of Streptomyces with plant soil was also observed by independent pyrosequencing results of cultures. The bacterial diversity of GA and CP was greater than those of YP and PD, and pyrosequencing detected diverse genera of Actinobacteria. The rain leaching model experiment suggested that geosmin and 2-MIB produced by the Actinomycetes could be released into the water systems by rainfall. The concentrations of the geosmin and 2-MIB leached from the planted soils were 2.6 times and 2.8 times greater than those from the unplanted soil. Moreover, the concentration of Streptomyces positively correlated with the amount of geosmin and 2-MIB (R2 = 0.809 and 0.847, respectively).

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