Hua Yan Water Corporation

Suzhou, China

Hua Yan Water Corporation

Suzhou, China
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Ge Y.,Harbin Institute of Technology | Yu S.,Harbin Institute of Technology | Shi W.,Harbin Institute of Technology | Yang F.,Hua Yan Water Corporation | Yin Q.,Hua Yan Water Corporation
Research Journal of Biotechnology | Year: 2017

Source water may be the main source of pathogen in drinking water. This study applied nextgeneration sequencing (NGS) to investigate the diversity of microorganism and pathogenic bacteria in source water in Tai Lake, China in winter. The physico-chemical characteristics of water were measured. Then the DNA of the samples was extracted and amplified by PCR. The PCR products were performed for pyrosequencing on a Miseq PE300 pyro sequencing platform. The result of 16s rDNA was anlayzed using the visualization software Gephi and the 16s rDNA gene pool of the known pathogenic bacteria was established. A total of 144,29216S rDNA gene sequences with average length of 395.66bps were obtained. These sequences revealed 580 operational taxonomic units (OTUs). The full length 16S rDNA gene database of common pathogenic bacteria was established. After blasting, 17 species of pathogenic bacteria were found. The most abundant potential human pathogenic bacteria were affiliated with B. tribocorum. The approaches used in this study could simultaneously detect most of the known potentially pathogenic bacteria in source water. The relationship between the microorganisms and source water in different periods would help us understand the diversity of microorganisms in source water in winter.

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