Abe S.,Iwate University |
Abe S.,Nichihara Research and Development Laboratories Inc |
Yamamoto K.-I.,Iwate University |
Yamamoto K.-I.,Chiyoda Corporation |
And 5 more authors.
Journal of Insect Biotechnology and Sericology | Year: 2014
An entomogenous fungus, Cordyceps militaris, grows on larvae and pupae of the silkworm, Bombyx mori. To overcome problems in the establishment of fungal infection caused by advancing pupal-adult development, we tested the effects of carbon dioxide (CO2) anesthesia. Pupae inoculated with C. militaris were held in CO2anesthesia for 2 days. Almost all pupae exposed to CO2were infected with C. militaris, but the rate of infection of chilled or untreated pupae decreased with increasing stage of pupal-adult development at inoculation. The results indicate that exposure of silkworm pupae to CO2enhances infection rate. This treatment will support the mass production of C. militaris for pharmacological uses. © 2014, Japanese Society of Sericultural Sciences. All rights reserved.