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Huang D.-P.,Wannan Medical University
Journal of Leukemia and Lymphoma | Year: 2013

Objective To explore the clinical characteristics, diagnosis, therapy and prognostic features of granulocytic sarcoma (GS). Methods Retrospective analysis was used to analyse 10 cases who were diagnosed with GS. Results Five patients were diagnosed with primary GS. GS was accompanied by APL in one case, CML in 3 cases and MDS in one case. Patients were treated with resection and chemotherapy, or imatinib treatment. Follow-up data were available from 9 patients, with 2 patients being still alive. The survival of one patient who received high-dose cytarabine chemotherapy was 66 months. The other 7 patients died of tumor-related diseases, and their survival ranged from 4 to 17 months. Conclusion GS is a rarely diagnosed disease. A correct diagnosis of GS depends on detailed morphological examination and immunohistochemical study. The clinical outcome of patients is poor, and AML-type chemotherapy is the proper treatment for GS. High-intensity chemotherapy and hematopoietic stem cell transplantation might improve long-term survival.


Gao Y.,Guangdong Pharmaceutical University | Huang Y.,Wannan Medical University | Zhang Y.,Yijishan Hospital of Wannan Medical University | Liu F.,Guangzhou University | And 9 more authors.
International Journal of Environmental Research and Public Health | Year: 2014

A community-based intervention study was conducted to assess a nutrition education intervention on western style fast food consumption among Chinese children and parents. Eight kindergartens from three district areas of Hefei City (a total of 1252 children aged 4-6 years and their parents) were randomly selected. Descriptive and analytical statistical methods were used to evaluate the baseline, midterm, and final western style fast food knowledge, attitude, and practice in both parents and children were used to identify and compare the knowledge, attitude, and practice in the parents and children. Parents and children were divided into "intervention" and "control" groups based on nutrition education status. Consumption of western style fast food at breakfast in Chinese children and parents is not high. The main reasons for this in children is that consumption of western style fast food is not viewed as "food", but rather as a "gift" or "interesting". The time of children's consumption of western style fast food is mostly likely to be in the weekends. The nutrition education modified the parents' western style fast food behavior (p < 0.01), although it did not change significantly in children. The healthy nutrition concept should be built up among Chinese, especially in children. Insights from the families provide leads for future research and ideas for the nutrition education. © 2014 by the authors; licensee MDPI, Basel, Switzerland.


Wang G.D.,Wannan Medical University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012

To study the effects of recombinant human bone morphogenetic protein-7 (rhBMP-7) on osteoblast differentiation of murine bone marrow mesenchymal stem cells (BMSCs). The BMSCs were collected from murine bone marrow by density gradient centrifugation. Cells were adherent cultured and expanded in vitro. RhBMP-7 was added into the culture medium of the 3rd passage BMSCs. Five days later, we performed alkaline phosphatase (ALP) staining and detected ALP activity and osteocalcin (OC) content. Osteoblast differentiation marker gene including OC and collage I (Col-I) were assayed by RT-PCR. Total protein was isolated and the secretion of Col-I in the cells was measured by Western blotting. The staining intensity of ALP in the group with rhBMP-7 was stronger than that in the negative control group. ALP activity and OC content of rhBMP-7 group were obviously higher than that of the negative control group. The mRNA of OC and Col-I and the protein of Col-I were highly expressed in the group induced by rhBMP-7. RhBMP-7 can induce murine BMSCs to differentiate into osteoblast in vitro.


Li C.-P.,Anhui University of Science and Technology | Li C.-P.,Anhui Medical University | Chen Q.,Anhui Medical University | Zhang X.-D.,Anhui Medical University | He L.-P.,Wannan Medical University
Journal of Pure and Applied Microbiology | Year: 2014

To investigate the species and breeding density of the acaroid mites in the stored dry fruits, and understand their diversity and community distribution. The stored dry fruits were collected from 30 stores and warehouses in China, and the mites were isolated from the previous samples. Twenty species of acaroid mites, belonging to 16 genera under 6 families, were identified from the total 49 stored dry fruit samples, in which the Tyrophagus putrescentiae Acarus siro, Charpoglyphus lactis and Caloglyphus berlesei are predominant. High breeding density of acaroid mites was found in the stored dry fruits. This indicates that the dried fruits in storages are seriously contaminated by the acaroid mites, and such infestation should be positively controlled to reduce the potential harm public health.


Huang Y.,Anhui University of Science and Technology | Huang Y.,Wannan Medical University | Zhang Y.,Yijishan Hospital of Wannan Medical University | Li C.,Anhui University of Science and Technology | And 6 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

To explore effects of natural crude extract of C. elegans on treatment of asthma. Method: Obtain crude extract of C. elegans from synchronically incubated C. elegans via centrifugation, washing and ultrasonic emulsification, etc.; measure C. elegans's protein molecular weight via SDS-polyacrylamide gel electrophoresis (SDS-PAG electrophoresis); construct animal models of asthma with 6-8-week-old BALB/c female mice sensitized by chicken ovalbumin (OVA); conduct immunotherapy on animals with asthma with different doses of mixture of C. elegans and OVA (COM) respectively; take PBS buffer group and OVA group as control groups; conduct inspection of cell factors and differential count of cells in serum IgE, IgG1 and IgG2a antibodies and bronchoalveolar lavage fluid (BALF) via enzyme linked immunosorbent assay (ELISA); and incise lung tissue for pathology observation. Result: C. elegans's protein molecular weight is about 50 kd. In bronchoalveolar lavage fluid (BALF) of OVA group, cell factors IL-5 and IL-13 are more than those in PBS buffer group, but IL-2 and IFN-γ are less than those in PBS buffer group; these differences are of statistical significance (P<0.05). Total cellular score and number of eosinophile granulocyte in BALF of OVA group are more than those in PBS buffer group (P<0.05), and the difference in serum IgE, IgG1 and IgG2a between these two groups is of statistical significance (P<0.05). For groups treatment by different doses of COM, cell factors IL-5 and IL-13 in bronchoalveolar lavage fluid (BALF) are less than those in OVA group, but IL-2 and IFN-γ are more than those in OVA group; these differences are of statistical significance (P<0.05). Total cellular score and number of eosinophile granulocyte in BALF of COM treatment groups are less than those in OVA group (P<0.05); serum IgE and IgG1 less than those in OVA group, but IgG2a is more than that in OVA group; these differences are of statistical significance (P<0.05). Conclusion: The natural crude extract of C. elegans has immunoregulation to animals with asthma.

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