Wakodo Co.

Chōfugaoka, Japan

Wakodo Co.

Chōfugaoka, Japan
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Fuyama Y.,Matsudo City Hospital | Fuyama Y.,Jikei University School of Medicine | Tonooka T.,Wakodo Co. | Wada Y.,Jikei University School of Medicine | And 2 more authors.
Tokyo Jikeikai Medical Journal | Year: 2013

To clarify the acquisition of the predominant Bifidobacterium strains in intestinal microbiota during infancy, we have examined the transmission between mother and child, focusing on the feces and breast milk of the mother. Because random amplification of polymorphic DNA showed that Bifidobacterium strains isolated from the feces of mother and child matched, we concluded that transmission occurred between mother and child. On the other hand, that Bifidobacterium strains were not isolated from breast milk suggests that intake of breast milk is not related to the acquisition of Bifidobacterium strains in the neonatal period. However, we recognize that transmission of bifidobacterial strains occurs. We conclude that administration of probiotics to pregnant women contributes to the acquisition of Bifidobacterium strains in the neonatal period.


Fuyama Y.,Matsudo City Hospital | Fuyama Y.,Red Cross | Tonooka T.,Wakodo Co. | Wada Y.,Jikei University School of Medicine | Ida H.,Jikei University School of Medicine
Tokyo Jikeikai Medical Journal | Year: 2012

The establishment and formation of normal intestinal microbiota in infants are effective for preventing infectious diseases and atopic dermatitis. To clarify the establishment and formation of the intestinal microbiota of healthy infants, vertical analysis is an appropriate method. To our knowledge, few long-term studies have examined intestinal microbiota in Japanese infants. With the culture method, we found that Enterobacteriaceae were predominant until the second day, and Bifidobacterium, Bacteroidaceae, and Enterococcus appeared by the fifth day. Bifidobacterium continued to predominant until the age of 2 years. With the species-specific polymerase chain reaction method, Bifibobacterium breve was detected at a high rate until the age of 2 years. In 2-year-old children the intestinal microbiota and the distribution of bifidobacterial species differed from those of adults. The factors that affected the establishment and formation of intestinal microbiota were administration of an antibiotic, severe diarrhea, and allergic disease in infancy. We obtained information about the establishment and formation of intestinal microbiota by vertical analysis of intestinal microbiota until the age of 2 years. These research findings can be used to further examine changes in intestinal microbiota with specific diseases.


The U.S. Federal Food, Drug, and Cosmetic Act (FFDCA) defines infant formula as "a food which purports to be or is represented for special dietary use solely as a food for infants by reason of its simulation of human milk or its suitability as a complete or partial substitute for human.”  Baby food and infant formula are specially designed by the manufactures to meet the nutritional requirements of the baby. Baby food and infant formula are generally fortified with required amount of proteins and iron, so that it properly helps in the body development and growth in the baby. Baby food and infant formula market is classified into various segment which includes baby meals, baby cereals, baby juice and baby cookie. There are mainly three forms of infant formula, which are powder, liquid concentrate, and ready-to-feed. Powder is the least expensive form of infant formulas and it is used by mixing with water before feeding to the infant. Liquid concentration is mixed with equal amount mixed with water before it is given to the infant. In case of ready-to-feed infant formula, no mixing up of any liquid is required and can directly used for feeding purpose. Growing urbanization, rising disposable income level of individual and increasing population base are some of the key reason, which drive the baby food and infant formula market in all across the globe. Decline in birth rate in developing countries acts as a major challenge for the growth in baby food and infant formula market. Among various regions, Asia Pacific market is one of the prominent market for baby food and infant formula and this market is expected to witness fastest growth rates during forecasted period 2014- 2020. In Asia Pacific region, China and India holds the largest share in baby food and infant formula market. North America is one of the largest markets for baby food and infant formula. In North America region, the U.S accounts the largest market share in baby food and infant formula. According to a report, around 1 million infants in the U.S. intake baby food and infant formula post birth and this rises to 2.7 million by the time, when baby reach to three months. The global baby food and infant formula market is expected to grow in a single digit growth rate during forecasted period 2014- 2020. Some of the major companies operating in global baby food and infant formula market are  Abbott Laboratories, Danone SA, Bledina SA, H. J. Heinz Company, Hain Celestial Group, Ella's Kitchen Group Ltd, Hero Group, Beech-Nut Nutrition Corporation, Semper AB, Mead Johnson Nutrition Company, Morinaga Milk Industry Co., Ltd., Nestlé S.A., Gerber Products Company, Nurture Inc., Parent's Choice, Plum Organics, Synutra International Inc., Wakodo Co. Ltd., Ballamy Organics, Ella’s Kitchen, Friesland, HIPP, Inner Mongolia Yili Industrial Group Co. Ltd., Manna Foods, Meiji, Stonyfield Farm Inc. and Sprout Foods In.


Inoue K.,Ritsumeikan University | Tanada C.,University of Shizuoka | Hosoya T.,University of Shizuoka | Yoshida S.,University of Shizuoka | And 6 more authors.
Journal of the Science of Food and Agriculture | Year: 2016

BACKGROUND: The challenge in developing analytical assessment of unexpected excess contaminations in infant formula has been the most significant project to address the widespread issue of food safety and security. Foodomics based on metabolomics techniques provides powerful tools for the detection of tampering cases with intentional contaminations. However, the safety and risk assessments of infant formula to reveal not only the targeted presence of toxic chemicals, but also molecular changes involving unexpected contaminations, have not been reported. In this study, a huge amount of raw molecularly based signals from infant formula was analysed using reversed phase and hydrophilic interaction chromatography with time-of-flight MS (LC-MS) and 1H nuclear magnetic resonance (NMR) and then processed by a principal component analysis (PCA). RESULTS: PCA plots visualised signature trends in the complex signal-data batches from each excess contamination of detectable chemicals by LC-MS and NMR. These trends in the different batches from a portion of excess chemical contaminations such as pesticides, melamine and heavy metals and out-of-date products can be visualised from spectrally discriminated infant formula samples. CONCLUSION: PCA plots provide possible attempts to maximise the covariance between the stable lot-to-lot uniformity and excess exogenous contaminations and/or degradation to discriminate against the molecularly based signals from infant formulas. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry


Nadaoka I.,Wakodo Co. | Hatakeyama E.,Wakodo Co. | Tanada C.,University of Shizuoka | Sakamoto T.,University of Shizuoka | And 5 more authors.
Analytical Sciences | Year: 2014

We developed a rapid and useful routine screening assay for total L-carnitine content in various infant formulas and materials by liquid chromatography coupled with a tandem mass spectrometric method (LC-MS/MS) and alkaline hydrolysis. For separation of L-carnitine, a multi-mode octadecylsilane (ODS) column was used that contained ODS ligands, anion ligands, and cation ligands to avoid using ion-pairing agents. The stable isotope L-carnitine-d3 (m/z 165 → 103/85) was used in electrospray MS/MS in the multiple reaction monitoring mode with the ion transitions of m/z 162 →103/85 for detection and quantitation of L-carnitine. Alkaline hydrolysis of short/medium chain (C2 - C15) acyl-Lcarnitines in infant formula was analyzed by LC with quadrupole time-of-flight mass spectrometry (QTOF-MS). The majority of short/medium chain acyl-L-carnitines were hydrolyzed to free L-carnitine. The overall standard deviations for L-carnitine in infant formula, follow-up formula and raw materials ranged from 2.1 to 4.0. The overall mean recoveries ranged from 90.2 to 94.2%. 2014 © The Japan Society for Analytical Chemistry.


Mizunoya W.,Kyushu University | Miyahara H.,Kyushu University | Okamoto S.,Kyushu University | Akahoshi M.,Kyushu University | And 13 more authors.
PLoS ONE | Year: 2015

A recent study demonstrated a positive effect of apple polyphenol (APP) intake on muscle endurance of young-adult animals. While an enhancement of lipid metabolism may be responsible, in part, for the improvement, the contributing mechanisms still need clarification. Here we show that an 8-week intake of 5% (w/w) APP in the diet, up-regulates two features related to fiber type: the ratio of myosin heavy chain (MyHC) type IIx/IIb and myoglobin protein expression in plantaris muscle of 9-week-old male Fischer F344 rats compared to pair-fed controls (P < 0.05). Results were demonstrated by our SDS-PAGE system specialized for MyHC isoform separation and western blotting of whole muscles. Animalgrowth profiles (food intake, body-weight gain, and internal-organ weights) did not differ between the control and 5% APP-fed animals (n = 9/group). Findings may account for the increase in fatigue resistance of lower hind limb muscles, as evidenced by a slower decline in the maximum isometric planter-flexion torque generated by a 100-s train of electrical stimulation of the tibial nerve. Additionally, the fatigue resistance was lower after 8 weeks of a 0.5% APP diet than after 5% APP, supporting an APP-dose dependency of the shift in fibertype composition. Therefore, the present study highlights a promising contribution of dietary APP intake to increasing endurance based on fiber-type composition in rat muscle. Results may help in developing a novel strategy for application in animal sciences, and human sports and age-related health sciences. © 2015 Mizunoya et al.


In this study including the field of metabolomics approach for food, the evaluation of untargeted compounds using HILIC-ESI/TOF/MS and multivariate statistical analysis method is proposed for the assessment of classification, contamination and degradation of infant formula. HILIC mode is used to monitor more detected numbers in infant formulas in the ESI-positive scan mode than the reversed phase. The repeatability of the non-targeted contents from 4 kinds of infant formulas based on PCA was less than the relative standard deviation of 15% in all groups. The PCA pattern showed that significant differences in the classification of types and origins, the contamination of melamine and the degradations for one week were evaluated using HILIC-ESI/TOF/MS. In the S-plot from the degradation test, we could identify two markers by comparison to standards as nicotinic acid and nicotinamide. With this strategy, the differences from the untargeted compounds could be utilized for quality and safety assessment of infant formula.


PubMed | Wakodo Co.
Type: Journal Article | Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry | Year: 2014

We developed a rapid and useful routine screening assay for total L-carnitine content in various infant formulas and materials by liquid chromatography coupled with a tandem mass spectrometric method (LC-MS/MS) and alkaline hydrolysis. For separation of L-carnitine, a multi-mode octadecylsilane (ODS) column was used that contained ODS ligands, anion ligands, and cation ligands to avoid using ion-pairing agents. The stable isotope L-carnitine-d3 (m/z 165 103/85) was used in electrospray MS/MS in the multiple reaction monitoring mode with the ion transitions of m/z 162 103/85 for detection and quantitation of L-carnitine. Alkaline hydrolysis of short/medium chain (C2 - C15) acyl-L-carnitines in infant formula was analyzed by LC with quadrupole time-of-flight mass spectrometry (QTOF-MS). The majority of short/medium chain acyl-L-carnitines were hydrolyzed to free L-carnitine. The overall standard deviations for L-carnitine in infant formula, follow-up formula and raw materials ranged from 2.1 to 4.0. The overall mean recoveries ranged from 90.2 to 94.2%.


PubMed | University of Shizuoka, Ritsumeikan University and Wakodo Co.
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2016

The challenge in developing analytical assessment of unexpected excess contaminations in infant formula has been the most significant project to address the widespread issue of food safety and security. Foodomics based on metabolomics techniques provides powerful tools for the detection of tampering cases with intentional contaminations. However, the safety and risk assessments of infant formula to reveal not only the targeted presence of toxic chemicals, but also molecular changes involving unexpected contaminations, have not been reported. In this study, a huge amount of raw molecularly based signals from infant formula was analysed using reversed phase and hydrophilic interaction chromatography with time-of-flight MS (LC-MS) and (1) H nuclear magnetic resonance (NMR) and then processed by a principal component analysis (PCA).PCA plots visualised signature trends in the complex signal-data batches from each excess contamination of detectable chemicals by LC-MS and NMR. These trends in the different batches from a portion of excess chemical contaminations such as pesticides, melamine and heavy metals and out-of-date products can be visualised from spectrally discriminated infant formula samples.PCA plots provide possible attempts to maximise the covariance between the stable lot-to-lot uniformity and excess exogenous contaminations and/or degradation to discriminate against the molecularly based signals from infant formulas. 2015 Society of Chemical Industry.

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