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The object of the present invention is to provide a novel primer for use in the detection of Mycobacterium avium (M. avium), and a method for detection of M. avium conveniently, rapidly and with high precision by using the primer. More specifically, disclosed are an oligonucleotide which comprises a part or an entire of the nucleotide sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:130, SEQ ID NO:131, SEQ ID NO:132, SEQ ID NO:133, SEQ ID NO:134, SEQ ID NO:135 and SEQ ID NO:136, or a part or an entire of the sequence complementary to the nucleotide sequence, and which is capable of hybridizing with the nucleotide sequence for a Mycobacterium avium gene; a primer and a probe for use in the detection of M. avium, which comprises the oligonucleoride; and a method for detection of M. avium using the primer and/or probe.


Patent
Wako Pure Chemical Industries Ltd. and Wako Life science Inc. | Date: 2017-02-15

A method for purifying polynucleic acids is disclosed, comprising adsorbing the polynucleic acids to a filter, washing the filter with a water-immiscible solution, and eluting the polynucleic acids from the filter. The method can be used to prepare purified RNA and/or DNA.


Patent
Wako Pure Chemical Industries Ltd. and Rhombic Corporation | Date: 2017-05-10

Provided are: a graft polymer and a colored resin that are free from color migration or fading caused by bleed-out after coloring, which is a defect of a dye, without impairing vivid color development and transparency of the dye; a method for producing the same; and a resin composition containing the colored resin. The present invention relates to: (1) a graft polymer that has a polyolefin (A) as the main chain, and a polymer (B) polymerized a dye (b) having a radical reactive functional group, in a side chain; (2) the graft polymer which is characterized in that the dye (b) having the radical reactive functional group is a cationic rhodamine derivative, a cationic triarylmethane-type dye derivative or a cationic cyanine-type dye derivative, having an ethylenically unsaturated group; (3) a colored resin consisting of the graft polymer; as well as (4) a method for producing the colored resin, which is characterized by mixing the polyolefin (A), the dye (b) having the radical reactive functional group, and the radical reaction initiator (C), and subjecting a radical reaction of the mixture; and the like.


Patent
Wako Pure Chemical Industries Ltd. and Rhombic Corporation | Date: 2015-07-01

The present invention relates to: (1) a graft polymer that has a polyolefin (A) as the main chain, and a polymer (B) polymerized a dye (b) having a radical reactive functional group, in a side chain; (2) the graft polymer which is characterized in that the dye (b) having the radical reactive functional group is a cationic rhodamine derivative, a cationic triarylmethane-type dye derivative or a cationic cyanine-type dye derivative, having an ethylenically unsaturated group; (3) a colored resin consisting of the graft polymer; as well as (4) a method for producing the colored resin, which is characterized by mixing the polyolefin (A), the dye (b) having the radical reactive functional group, and the radical reaction initiator (C), and subjecting a radical reaction of the mixture; and the like.


Patent
Wako Pure Chemical Industries Ltd. | Date: 2017-06-28

The purpose of the present invention is to provide a method for determining cancer, and a cancer marker enabling determination of canceration of cells in good precision, and enabling determination of canceration of various cells. The present invention relates to a cancer marker, consisting of a portion of or all of the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell, and being continuous nucleotide sequences comprising at least the nucleotide sequence represented by SEQ ID NO: 3, wherein 30% or more of cytosines of the -CG- sequences present in said nucleotide sequence are methylated, a cancer marker, consisting of a portion of or all of the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell, and being continuous nucleic sequences comprising at least the nucleotide sequence represented by SEQ ID NO: 2, wherein 30% or more of cytosines of the -CG- sequences present in said nucleotide sequence are methylated, and a method for determining canceration of a cell on the basis of methylation ratio, wherein said methylation ratio is measured by a bisulfite reaction for a portion of or all of cytosines of the -CG- sequences present in the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell.


Provided is a method which is capable of determining, at an initial stage of differentiation, the differentiated state of undifferentiated stem cells. The present invention relates to a method for determining a differentiated state of a cell comprising detecting expression of FOXB2 gene of a stem cell, and determining the differentiated state based on the result, and a differentiation marker selected from mRNA or protein derived from the FOXB2 gene. The present invention is applicable to quality management of the stem cells or to methods for preparation and isolation of the differentiated cells. Further, because the differentiated cells can be determined at an initial stage of culture, the present invention is useful for an early stage screening of cells and for quality management of the stem cells, and reduction in culturing period and cost reduction in expenditure regarding a culture medium, or the like, can be expected.


Patent
Wako Pure Chemical Industries Ltd. and Tokyo University of Science | Date: 2016-05-11

It is a subject of the present invention to provide a base generator which has high solubility to general-purpose organic solvents, can dissolve directly into a base-reactive compound, such as an epoxy-based compound, further is provided with both performance of high heat resistance and low nucleophilicity, and generates a strong base, a base-reactive composition comprising the base generator and a base-reactive compound, as well as a method for generating a base, etc. The present invention relates to a compound represented by the general formula (A), a base generator comprising the compound, a base-reactive composition which comprises the base generator and a base-reactive compound, as well as a method for generating a base, etc.^(1) to R^(5) each independently represent a hydrogen atom; an alkyl group; an aryl group which may have a substituent; or an arylalkyl group which may have a substituent, R^(6) represents a hydrogen atom; an alkyl group which may have a substituent; an alkenyl group; an alkynyl group; an aryl group which may have a substituent; or an arylalkyl group which may have a substituent, R^(7) represents a hydrogen atom; an alkyl group which may have an amino group; an aryl group which may have a substituent; or an arylalkyl group which may have a substituent, and Z^(-) represents an anion derived from a carboxylic acid having a specific structure.)


Patent
Wako Pure Chemical Industries Ltd. and Osaka University | Date: 2016-01-06

The present invention provides a breast cancer marker selected from the group consisting of fucosylated hornerin, fucosylated Zn--2-glycoprotein, fucosylated Ig -1 chain C region, and fucosylated desmoplakin; a method for determining breast cancer comprising detecting the breast cancer marker in a sample, and determining on the basis of the results of the results; and a kit to be used for the determination.


Patent
Wako Pure Chemical Industries Ltd. | Date: 2016-11-30

An object of the present invention is to provide a compound which is capable of attaining a composition having high storage stability without reacting with a base-reactive compound, even in the case of storage for a long period of time in a mixed state with the base-reactive compound, such as an epoxy-based compound, as well as capable of generating a strong base (guanidines, biguanides, phosphazenes or phosphoniums) by irradiation of light (active energy rays) or heating; a base generator comprising the compound; and a base-reactive composition comprising the base generator and the base-reactive compound. The present invention relates to the compound represented by the general formula (A); the base generator comprising the compound; and the base-reactive composition comprising the base generator and the base-reactive compound.^(1) represents an alkyl group; an arylalkynyl group which may be substituted with a halogen atom, an alkyl group, an alkoxy group, or an alkylthio group; an alkenyl group; a 2-furylethynyl group; a 2-thiophenylethynyl group; or a 2,6-dithianyl group; R^(2) to R^(4) each independently represent an alkyl group; an arylalkynyl group which may be substituted with a halogen atom, an alkyl group, an alkoxy group, or an alkylthio group; the aryl group which may be substituted with a halogen atom, an alkyl group, an alkoxy group, or an alkylthio group; a furanyl group; a thienyl group; or an N-alkyl-substituted pyrrolyl group; Z^(+) represents an ammonium cation having a guanidinium group, a biguanidium group or a phosphazenium group, or a phosphonium cation.)


Patent
Wako Pure Chemical Industries Ltd. | Date: 2016-02-17

It is a subject of the present invention to provide a cleaning agent for a substrate having a metal wiring, and a cleaning method for a semiconductor substrate comprising that the cleaning agent is used, by which following effects (1) to (5) are obtained, in a cleaning process after chemical mechanical polishing (CMP) in a manufacturing process of a semiconductor device. (1) Residues of fine particles (polishing agents) used in the CMP process, fine particles (metal particles) derived from a polished metal, an anticorrosive, and the like, can be removed sufficiently. (2) A coating film (protective film: oxidation resistant film) on a surface of the metal wiring, containing a complex between an anticorrosive, such as benzotriazole or quinaldic acid, and a surface metal of the metal wiring, formed in the CMP process, can be removed (stripped) sufficiently. (3) An oxide film containing a metal oxide can be formed after removal (stripping) of the coating film. (4) A semiconductor substrate can be obtained stably for a long period of time, without impairing flatness of the surface of the metal wiring (the surface of the oxide film containing the metal oxide), even leaving a substrate after the cleaning process after the CMP. (5) It is hard to deteriorate even after using the cleaning agent for a long period of time. The present invention relates to a cleaning agent for a substrate having a metal wiring, comprising an aqueous solution containing (A) carboxylic acid having a nitrogen-containing heterocyclic ring and (B) alkylhydroxylamine, and having a pH of 10 or higher, as well as a cleaning method for a semiconductor substrate, comprising that the cleaning agent is used.

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