Wako Pure Chemical Industries Ltd

Kawagoe, Japan

Wako Pure Chemical Industries Ltd

Kawagoe, Japan
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Patent
Wako Pure Chemical Industries Ltd. | Date: 2017-01-11

It is an object of the present invention to provide a cyanine-based coloring composition having higher heat resistance, as compared with a conventional coloring composition. The present invention relates to a compound represented by the following general formula (1), a polymer having a monomer unit derived from the compound, along with a coloring composition, a coloring composition for a color filter, and a colored resin comprising the above-described compound or the above-described polymer.


Patent
Wako Pure Chemical Industries Ltd. | Date: 2017-02-01

A colored resin composition using a conventional rhodamine derivative has not provided heat resistance in a practical range. Therefore, it is an object of the present invention to provide a coloring composition having higher heat resistance as compared with the conventional coloring composition. The present invention relates to a compound represented by the following general formula (1), and a polymer having a monomer unit derived from the compound, and the like._(1) to R_(4) each independently represent a hydrogen atom, an alkyl group, and the like; R_(7) represents a hydrogen atom or a methyl group; R_(8) and R_(9) each independently represent a hydrogen atom or an alkyl group; Y represents a nitrogen atom or a group represented by the following formula (1-1):_(1) represents an alkylene group which has at least one group selected from -O-, -OCO-, -COO-, -NHCO-, -CONH-, -NHCONH- and an arylene group in the chain, and the like; A_(2) represents -NH- or -O-; An^(-) represents an anion containing an aryl group having an electron-withdrawing substituent, and the like; n represents an integer of 0 to 3; R_(8) and R_(9) may form a cyclic structure of a 5 to 6 membered ring together with -N-(CH_(2))_(n)-Y bonding thereto.)


Patent
Wako Pure Chemical Industries Ltd. | Date: 2017-04-05

Using the colored resin composition using a conventional triarylmethane derivative, heat resistance within a practical range has not been obtained. Therefore, it is an object of the present invention to provide a colored composition having higher heat resistance as compared with the conventional colored composition. The present invention relates to a compound represented by the following general formula (1), and a polymer having a monomer unit derived from the compound, and the like._(1) to R_(4) each independently represent an alkyl group or the like; R_(5) to R_(7) each independently represent a hydrogen atom or a methyl group; n pieces of R_(8) each independently represent a halogen atom, an alkyl group, an aryl group, a hydroxy group, a nitro group, a sulfo group, an alkoxy group, or the like; and n represents an integer of 0 to 4. A_(1) represents an alkylene group, or the like; A_(2) represents -NH- or -O-. An^(-) represents an anion containing an aryl group having an electron-withdrawing substituent, a sulfonyl group having an electron-withdrawing substituent, or a halogenated alkyl group.)


Patent
Wako Pure Chemical Industries Ltd. and Tokyo University of Science | Date: 2017-03-01

It is an object of the present invention to provide an electrode having superior charge-discharge capacity, even in the case of using an active material containing silicon. The present invention relates to a binder for a lithium cell comprises a polymer having a monomer unit derived from acrylic acid, and 1 to 2 kinds of monomer units derived from the compound shown by the general formula (I), the general formula (II), or the general formula (III), as constituents, and cross-linked by a cross-linking agent selected from the compounds described by the following general formulae [1] to [13], and the polymer described by the following general formula [14], a composition for producing an electrode of a lithium cell comprising 1) an active material containing silicon, 2) a conductive assistant, and 3) the above-described binder for a cell, and an electrode for a lithium cell having 1) an active material containing silicon, 2) a conductive assistant, 3) the above-described binder for a cell, and (4) a current collector.


Patent
Wako Pure Chemical Industries Ltd. | Date: 2017-03-08

The present invention addresses the problem of providing a method for specifically measuring CK-MB while avoiding an influence of CK-BB. That is, the present invention relates to A method for measuring CK-MB in a sample, comprising: a step for reacting the sample with a first antibody against CK-MB, a second antibody against CK-MB which recognizes an epitope different from that recognized by the first antibody, and an anti-CK-B antibody which recognizes a region of the 1st to 100th amino acid from N-terminus of the amino acid sequence of the subunit B of creatine kinase (step 1); a step for measuring an optical change generated by the reaction (step 2); and a step for determining a quantity of CK-MB in the sample on the basis of the result obtained in the step 2 (step 3), and a kit to be used in the method, and a method for avoiding a influence of creatine kinase BB isozyme in measuring CK-MB, comprising treating a sample with the anti-CK-B antibody.


Provided is a method which is capable of determining, at an initial stage of differentiation, the differentiated state of undifferentiated stem cells. The present invention relates to a method for determining a differentiated state of a cell comprising detecting expression of FOXB2 gene of a stem cell, and determining the differentiated state based on the result, and a differentiation marker selected from mRNA or protein derived from the FOXB2 gene. The present invention is applicable to quality management of the stem cells or to methods for preparation and isolation of the differentiated cells. Further, because the differentiated cells can be determined at an initial stage of culture, the present invention is useful for an early stage screening of cells and for quality management of the stem cells, and reduction in culturing period and cost reduction in expenditure regarding a culture medium, or the like, can be expected.


Patent
Wako Pure Chemical Industries Ltd. | Date: 2017-06-28

The purpose of the present invention is to provide a method for determining cancer, and a cancer marker enabling determination of canceration of cells in good precision, and enabling determination of canceration of various cells. The present invention relates to a cancer marker, consisting of a portion of or all of the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell, and being continuous nucleotide sequences comprising at least the nucleotide sequence represented by SEQ ID NO: 3, wherein 30% or more of cytosines of the -CG- sequences present in said nucleotide sequence are methylated, a cancer marker, consisting of a portion of or all of the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell, and being continuous nucleic sequences comprising at least the nucleotide sequence represented by SEQ ID NO: 2, wherein 30% or more of cytosines of the -CG- sequences present in said nucleotide sequence are methylated, and a method for determining canceration of a cell on the basis of methylation ratio, wherein said methylation ratio is measured by a bisulfite reaction for a portion of or all of cytosines of the -CG- sequences present in the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell.


Patent
Wako Pure Chemical Industries Ltd. and Wako Life science Inc. | Date: 2017-02-15

A method for purifying polynucleic acids is disclosed, comprising adsorbing the polynucleic acids to a filter, washing the filter with a water-immiscible solution, and eluting the polynucleic acids from the filter. The method can be used to prepare purified RNA and/or DNA.


Patent
Wako Pure Chemical Industries Ltd. and Rhombic Corporation | Date: 2017-05-10

Provided are: a graft polymer and a colored resin that are free from color migration or fading caused by bleed-out after coloring, which is a defect of a dye, without impairing vivid color development and transparency of the dye; a method for producing the same; and a resin composition containing the colored resin. The present invention relates to: (1) a graft polymer that has a polyolefin (A) as the main chain, and a polymer (B) polymerized a dye (b) having a radical reactive functional group, in a side chain; (2) the graft polymer which is characterized in that the dye (b) having the radical reactive functional group is a cationic rhodamine derivative, a cationic triarylmethane-type dye derivative or a cationic cyanine-type dye derivative, having an ethylenically unsaturated group; (3) a colored resin consisting of the graft polymer; as well as (4) a method for producing the colored resin, which is characterized by mixing the polyolefin (A), the dye (b) having the radical reactive functional group, and the radical reaction initiator (C), and subjecting a radical reaction of the mixture; and the like.


Patent
Wako Pure Chemical Industries Ltd. and Fujifilm Co. | Date: 2017-09-27

[Objective] A specimen is efficiently disrupted within a short period of time, in a specimen disrupting apparatus and a specimen disrupting method that rotate a container having the specimen, a great number of small diameter beads, and a large diameter bead stored therein. [Constitution] A solution (S) that includes a specimen, a great number of small diameter beads (SB), and a large diameter bead (BB) are stored in a container (2). A drive unit (40) rotates the lower portion of the container (2) having the above components stored therein. A control unit (70) controls the drive unit (40) such that the lower portion of the container (2) is rotated at two or more different rotational speeds which change continuously.

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