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Apeldoorn, Netherlands

de Klerk G.-J.,WageningenUR Plant Breeding | ter Brugge J.,WageningenUR Plant Breeding
Propagation of Ornamental Plants | Year: 2010

Alstroemeria rhizomes were micropropagated on semi-solid medium (AM) and in liquid medium (LM). In LM, growth was much enhanced (ca. 70%). Adequate gas exchange was crucial. This was obtained by agitation and in static medium by a sufficient large contact area of the explant and the gaseous atmosphere of the headspace. The medium became rapidly depleted with respect to both organic and inorganic nutrients. Manual addition of extra medium ingredients during the culture enhanced growth. Extra nutrients were also successfully administered via slow-release tools. Two types of slow-release tools were used, Osmocote for inorganic nutrients and newly developed castings for sucrose. Addition of extra 6-benzylaminopurine (BAP) during the culture stimulated the outgrowth of lateral rhizomes. The extra BAP could be included in the slow-release castings for sucrose. Source

Rojas-Martinez L.,WageningenUR Plant Breeding | Visser R.G.F.,WageningenUR Plant Breeding | de Klerk G.-J.,WageningenUR Plant Breeding
Propagation of Ornamental Plants | Year: 2010

Hyperhydricity (also known as 'vitrification') is a physiological disorder in tissue-cultured plant material, which causes a reduction of propagation and death of tissues when transferred to ex vitro conditions. A plethora of causes has been mentioned in literature. We consider this disorder as the result of the stressful conditions brought about by waterlogging of the apoplast. This causes hypoxia and thereby leads to severe oxidative stress. We argue that hyperhydric features like vitreous appearance and wrinkled leaves are secondary events resulting from waterlogging of the apoplast. With the use of readily available technologies, the molecular components of hyperhydricity can be dissected. Source

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