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Fremont, CA, United States

Rivers A.R.,Joint Genome Institute | Burns A.S.,University of Georgia | Burns A.S.,Georgia Institute of Technology | Chan L.-K.,WaferGen Biosystems | Moran M.A.,University of Georgia
Frontiers in Microbiology | Year: 2016

In oligotrophic ocean waters where bacteria are often subjected to chronic nutrient limitation, community transcriptome sequencing has pointed to the presence of highly abundant small RNAs (sRNAs). The role of sRNAs in regulating response to nutrient stress was investigated in a model heterotrophic marine bacterium Ruegeria pomeroyi grown in continuous culture under carbon (C) and nitrogen (N) limitation. RNAseq analysis identified 99 putative sRNAs. Sixty-nine were cis-encoded and located antisense to a presumed target gene. Thirty were trans-encoded and initial target prediction was performed computationally. The most prevalent functional roles of genes anti-sense to the cis-sRNAs were transport, cell-cell interactions, signal transduction, and transcriptional regulation. Most sRNAs were transcribed equally under both C and N limitation, and may be involved in a general stress response. However, 14 were regulated differentially between the C and N treatments and may respond to specific nutrient limitations. A network analysis of the predicted target genes of the R. pomeroyi cis-sRNAs indicated that they average fewer connections than typical protein-encoding genes, and appear to be more important in peripheral or niche-defining functions encoded in the pan genome. © 2016 Rivers, Burns, Chan and Moran. Source


Trademark
WaferGen Biosystems | Date: 2015-09-28

LABORATORY EQUIPMENT FOR SCIENTIFIC AND RESEARCH USE.


Trademark
WaferGen Biosystems | Date: 2008-03-28

laboratory instruments for use in performing polymerase chain reaction (PCR); laboratory instruments for use in performing polymerase chain reaction (PCR), namely, thermal cyclers; laboratory apparatus and instruments, namely, real-time thermal cyclers used in gene expression, genotyping and life sciences all for use in research. medical devices for use in performing polymerase chain reaction (PCR); thermal cyclers; medical devices used in gene expression, genotyping and life sciences, all for use in clinical, medical or diagnostic applications.


Trademark
WaferGen Biosystems | Date: 2011-01-18

Laboratory equipment, namely, micro-incubators and micro-incubator systems comprised of micro-incubators, an electronic instrument for controlling the micro-incubator, tubing, glass bottles, and/or computer software, all for use in growing and differentiating cells all for use in research; laboratory instruments for use in performing polymerase chain reaction (PCR) for scientific and research use; laboratory equipment for scientific and research use, namely, thermal cyclers; laboratory instruments, namely, real-time thermal cyclers used in gene expression, genotyping and life sciences all for use in research; laboratory equipment, namely, micro-incubators and micro-incubator systems comprised of micro-incubators, an electronic instrument for controlling the micro-incubator, tubing, glass bottles, and/or computer software, all for use in growing and differentiating cells all for use in clinical, medical or diagnostic applications.


Trademark
WaferGen Biosystems | Date: 2009-05-05

Laboratory equipment, namely, micro-incubators for use in growing and differentiating cells and micro-incubation systems comprised of micro-incubators and instruments for use in growing and differentiating cells, sold as a unit, all for use in research. Laboratory equipment, namely, micro-incubators for use in growing and differentiating cells and micro-incubation systems comprised of micro-incubators and instruments for use in growing and differentiating cells, sold as a unit, all for use in clinical, medical or diagnostic applications.

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