Waclaw Dabrowski Institute of Agricultural and Food Biotechnology in Warsaw

Warsaw, Poland

Waclaw Dabrowski Institute of Agricultural and Food Biotechnology in Warsaw

Warsaw, Poland
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Porebska I.,Waclaw Dabrowski Institute of Agricultural and Food Biotechnology in Warsaw | Sokolowska B.,Waclaw Dabrowski Institute of Agricultural and Food Biotechnology in Warsaw | Sokolowska B.,Polish Academy of Sciences | Wozniak L.,Waclaw Dabrowski Institute of Agricultural and Food Biotechnology in Warsaw | Laniewska-Trokenheim L.,University of Warmia and Mazury
Polish Journal of Natural Sciences | Year: 2016

Alicyclobacillus acidoterrestris (AAT) is an acidothermophilic spore forming bacterium that causes the contamination of pasteurized fruit and vegetable juices. Since it survives typical heat treatment, the use of more effective techniques, such as supercritical carbon dioxide (SCCD), are considered for preserving juices. Dipicolinic acid (DPA) is a universal component of bacterial spores and its release can serve as an indicator of spore germination. The aim of this study was to determine the relationship between the release of DPA and the germination of AAT spores, initiated by SCCD. aSamples of the spores of two AAT strains suspended in apple juice and pH 4.0 and pH 7.0 McIlvain buffers were treated with pressure of 10-60 MPa, at a temperature of 35-75oC for 30 min. The results showed that some of the process parameters, mainly the temperature and pH, strongly affected spore germination. The amount of released DPA correlated strongly (R = 0.928) to the number of germinated AAT spores.

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