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Singh P.,Vyas Dental College and Hospital | Dutta S.R.,M B Kedia Dental College | Guha D.,University of Calcutta
Saudi Journal of Gastroenterology | Year: 2015

Background/Aims: Serotonin (5-hydroxytryptamine; 5-HT) released from enterochromaffin (EC) cells in gastric mucosa inhibits gastric acidity by increasing the gastric mucus secretion. In the present study, we evaluated the effect of aqueous extract of Aegle marmelos (AM) ripe fruit pulp (250 mg/kg body weight) on mean ulcer index (MUI), EC cells, 5-HT content, and adherent mucosal thickness of ulcerated gastric tissue in adult albino rats. Material and Methods: Ulceration was induced by using aspirin (500 mg/kg, p.o.), cerebellar nodular lesion and applying cold-restraint stress. Results: In all cases increased MUI in gastric tissue along with decreased EC cell count was observed with concomitant decrease of 5-HT content and adherent mucosal thickness (P < 0.05). Pretreatment with AM for 14 days decreased MUI, increased EC cell count, and 5-HT content as well as adherent mucosal thickness in all ulcerated group (P < 0.05). Conclusion: AM produces gastric mucosal protection mediated by increased EC cell count and 5-HT levels.


Srivastava P.,CSIR - Central Electrochemical Research Institute | Upreti D.K.,CSIR - Central Electrochemical Research Institute | Dhole T.N.,Institute of Medical science | Srivastava A.K.,Saraswati Dental and Medical College | Nayak M.T.,Vyas Dental College and Hospital
Interdisciplinary Perspectives on Infectious Diseases | Year: 2013

Context. Usnea ghattensis G. Awasthi (Usneaceae) endemic fruticose lichen found growing luxuriantly in Northern Western Ghats of India, it also contains Usnic acid as a major chemical and tested against some human pathogenic bacteria. Objective. To explore antimicrobial properties of Usnea ghattensis against some human pathogenic bacteria. Materials and Methods. The lichen was extracted in acetone, methanol, and ethanol. In vitro antimicrobial activity was tested initially by Kirby-Bauer technique of disc diffusion method and was confirmed by minimum inhibitory concentration using Broth microdilution method according to the NCCLS guidelines. Results. Ethanol extract was most effective against Bacillus cereus and Pseudomonas aeruginosa with a zone of inhibition 29.8 ± 0.6 mm and 12.3 ± 0.5 mm diameters at a concentration of 0.2 mg/mL. Acetone and methanol extract demonstrated almost similar activity against Staphylococcus aureus and the zone of inhibition was 24.6 ± 0.5 and 24.7 ± 0.4 mm. Only methanol extract was showing activity against Streptococcus faecalis with a 13.5 ± 0.8 mm zone. MIC value noted against Staphylococcus aureus and Streptococcus faecalis was 6.25 g/mL and 25 g/mL, whereas against Bacillus cereus and Pseudomonas aeruginosa, MIC calculated was 3.125 g/mL and 200 g/mL, respectively. Conclusion. The present study demonstrates the relatively higher activity of this lichen against not only gram (+) but significantly also against gram (-) bacteria. This indicates that this lichen might be a rich source of effective antimicrobial agents. © 2013 Priya Srivastava et al.


Verma Dr. D.K.,Vyas Dental College and Hospital
Oral Surgery | Year: 2012

Ankylosis of the temporomandibular joint is a very debilitating condition. A case report of a 12-year-old girl with ankylosis between the mandibular notch and the skull base is presented. Trauma was identified as the probable cause. Al Kayat and Bramley approach followed by osteotomy and lateral swing of the zygomatic arch gained extraoral access to the site. Osteotomy of the bony mass was done between the mandibular notch and the skull base. The patient had good mouth opening at 1-year follow up. © 2012 John Wiley&Sons A/S.


Gupta G.,Vyas Dental College and Hospital | Mitra D.,Vyas Dental College and Hospital | Ashok K.P.,Vyas Dental College and Hospital | Gupta A.,Vyas Dental College and Hospital | And 3 more authors.
Journal of Periodontology | Year: 2014

The aerosol generated by an ultrasonic scaler contains microorganisms that can penetrate into the body through the respiratory system of dental surgeons and patients. The aim of this pilot study is to evaluate and compare the efficacy of commercially available preprocedural mouthrinses containing 0.2% chlorhexidine gluconate, an herbal mouthwash, and water in reducing the levels of viable bacteria in aerosols. Methods: This single-center, double-masked, placebocontrolled, randomized, three-group parallel design was conducted over a period of 45 days. Twenty-four patients with chronic periodontitis were divided randomly into three groups (A, B, and C) of eight patients each to receive 0.2% chlorhexidine gluconate, herbal mouthwash, and water, respectively, as a preprocedural rinse. The aerosol produced by the ultrasonic unit was collected at patient's chest area, doctor's chest area, and assistant's chest area on blood agar plates in all three groups. The blood agar plates were incubated at 37°C for 48 hours, and the total number of colony-forming units (CFUs) was counted and statistically analyzed. Results: The results showed that CFUs in groups A and B were significantly reduced compared with group C, P <0.001 (analysis of variance). Also, CFUs in group A were significantly reduced compared with group B, P <0.05 (independent t-test). The numbers of CFUs were highest at the patient's chest area and lowest at the assistant's chest area. Conclusion: This study suggests that a routine preprocedural mouthrinse could eliminate the majority of bacterial aerosols generated by the use of an ultrasonic unit, and that 0.2% chlorhexidine gluconate is more effective than herbal mouthwash. J Periodontol 2014;85:562-568. © 2013 American Academy of Periodontology.


Nayak M.T.,Vyas Dental College and Hospital | Singh A.,Vyas Dental College and Hospital | Desai R.S.,Nair Hospital Dental College | Vanaki S.S.,PMNM Dental College and Hospital
Journal of Cancer Epidemiology | Year: 2013

Background. Oral submucous fibrosis (OSF), a precancerous condition, is characterized by abnormal accumulation of collagen fibers in oral submucosa. Vimentin is a Class 2 intermediate filament (IF) and primarily expressed in cells of mesenchymal origin. Vimentin is also found to be involved in cell growth, cell cycling, and tumour differentiation. Objective. The purpose of the study was to compare the expression of vimentin in various histological grades of OSF. Materials and Methods. To assess the immunohistochemical expression of vimentin in 20 mild cases of OSF, 20 severe cases of OSF, and ten cases of normal oral buccal mucosa. Results. The overall staining intensity of vimentin significantly increased statistically (P<0.01) in OSF cases over normal control. A significant increase in the staining intensity of vimentin was also noted in the fibroblasts of severe cases of OSF (P=0.03). Conclusion. Considering the marked vimentin expression in the present study, future studies should include cytoskeleton IF and other filaments in the fibroblasts of OSF. © 2013 Meghanand T. Nayak et al.

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