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Mohan Gandhi B.,Sri Vasavi Institute of Pharmaceutical science | Lakshmana Rao A.,Vv Institute Of Pharmaceutical Science
Oriental Journal of Chemistry | Year: 2016

Novel HPTLC and stability indicating RP-HPLC methods were developed for simultaneous estimation of Moxifloxacin (MOX) and Dexamethasone (DEX) in ophthalmic dosage form. For HPTLC method, the separation was carried out on HPTLC aluminum plates using acetonitrile:water:ammonia (8:1:0.5 V/V/V) as mobile phase and developed plates were read at 266 nm. The drugs were resolved satisfactorily with Rf values of 0.09±0.01 and 0.74±0.01 for MOX and DEX, respectively. The RP-HPLC analysis is carried out on Shiseido C18 column (250 mm x 4.6 mm I.D., 5 μm), using 0.02M acetate buffer (pH is 4 adjusted with triethylamine) and acetonitrile in the ratio of 60:40 V/V with a flow rate of 1.2 m/min and the detection was carried out at 254 nm. The retention times were found to be 2.144±0.5 min and 4.732±0.5 min. for MOX and DEX respectively. Developed methods were validated as per ICH guidelines and were found to be within the limits. © 2016, Oriental Scientific Publishing Company. All rights reserved.


Rao A.L.,Vv Institute Of Pharmaceutical Science
Oriental Journal of Chemistry | Year: 2016

A novel approach was used to develop and validate a bioanalytical RP-HPLC method for the simultaneous estimation of Paracetamol and Cefixime in rabbit plasma using Cefaclor as internal standard. Evaluation of the content of drugs were done by employing a mixture of Phosphate buffer (pH6.4) and Acetonitrile (80:20, v/v) as the mobile phase and measure the absorbance at 245 nm for Paracetamol and Cefixime. Retention time was established to be 3.618 min for Cefaclor, 4.608 min for Paracetamol and 5.914min for Cefixime. The results shown that the analytical technique furnished here establishes acceptable accuracy and precision, shorter and easy sample preparation, reduced the complications for equipment on satisfactory analysis time.


Sharmila D.,Vv Institute Of Pharmaceutical Science
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2015

A simple, specific, precise, and accurate RP-HPLC method has been developed and validated for the estimation of Canagliflozin in bulk and tablet dosage form. Chromatographic separation was achieved on Hypersil BDS, C18 100 x 4.6 mm, 5 μ column using 0.1% ortho phosphoric buffer and acetonitrile (53:47) as mobile phase, water and aetonitrile (50:50) as diluent in isocratic mode. Flow rate of 1.1ml/min was optimized with detection wavelength at 240 nm. The retention time (Rt) was around 3.3±0.2 min. The method was validated with respect to specificity, selectivity, linearity, accuracy, precision, and robustness as per ICH gudieliness. The assay method was observed linear in the concentration range of 75-450 μg/ml with a Correlation coefficient (r2) 0.9999. The percentage recovery of active pharmaceutical ingredient from tablet dosage form ranged from 99.83-100.27%. The Limit of Detection and Limit of Quantification were found to be 0.23μg/ml and 0.7μg/ml, respectively. Stress conditions of degradation in acidic, alkaline, peroxide, thermal and UV radiation were studied and found Canagliflozin is sensitive to alkali degradation comparative to other stress conditions.


Vijaya Lakshmi M.,Andhra University | Seshagiri Rao J.V.L.N.,Andhra University | Lakshmana Rao A.,Vv Institute Of Pharmaceutical Science
Rasayan Journal of Chemistry | Year: 2010

An accurate and precise HPLC method was developed for the determination of rasagiline. Separation of the drug was achieved on a reverse phase C 18 column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 50:50 v/v. The flow rate was 0.5 ml/min and the detection wavelength was 210 nm. The linearity was observed in the range of 10-125 μg/ml with a correlation coefficient of 1.000. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of rasagiline in tablet dosage forms. © 2010 RASĀYAN. All rights reserved.


Bhaskara Raju V.,Sri Vasavi Institute of Pharmaceutical science | Lakshmana Rao A.,VV Institute of Pharmaceutical science
Rasayan Journal of Chemistry | Year: 2011

An accurate and precise RP-HPLC method was developed for the determination of perindopril in tablet dosage forms. Separation of die drug was achieved on a reverse phase Cig column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 65:35 v/v. The flow rate was 0.6 ml/min and die detection wavelength was 209 nm. The linearity was observed in the range of 20-100 ug/ml with a correlation coefficient of 0.9997. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of perindopril in tablet dosage forms. © 2011 RASAYAN.


Raju V.B.,Sri Vasavi Institute of Pharmaceutical science | Rao A.L.,Vv Institute Of Pharmaceutical Science
E-Journal of Chemistry | Year: 2012

An accurate and precise HPLC method was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C 8 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength was 215 nm. The linearity was observed in the range of 20-60 μg/mL with a correlation coefficient of 0.9992. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lisinopril in tablet dosage forms.


Vijaya Lakshmi M.,P.A. College | Rao J.V.L.N.S.,P.A. College | Rao L.,Vv Institute Of Pharmaceutical Science
E-Journal of Chemistry | Year: 2012

A rapid, sensitive and precise HPLC method was developed for the estimation of trospium chloride in pure and tablet dosage forms. Seperation of the drug was achieved on a reverse phase Azilent C 18 column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 60:40v/v. The flow rate was 1 ml/min and the detection wave length 215 nm. The linearity was found in the range of 10-150 μg/ml with a correlation coefficient of 1.0000. The proposed method was validated for its sensitivity, linearity, accuracy and precision. This method was employed for routine quality control analysis of trospium chloride in tablet dosage forms.

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