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Vishākhapatnam, India

Vani T.,Central Institute of Fisheries Education | Saharan N.,Central Institute of Fisheries Education | Roy S.D.,Central Institute of Fisheries Education | Ranjan R.,VRC of CMFRI | And 3 more authors.
Fish Physiology and Biochemistry

A 60-day experiment was carried out to study the effect of sub-lethal concentration of cypermethrin (1/10th of LC50) exposure on haematological and biochemical parameters of the Indian major carp, Catla catla fingerlings. Under exposure, the total erythrocyte count, total leucocyte count, haemoglobin content and haematocrit were decreased. All the studied serum parameters viz. total serum protein, albumin, globulin contents and albumin-globulin ratio were significantly decreased in cypermethrin-exposed fishes. A marked increase was recorded in alanine aminotransferase and aspartate aminotransferase activities in liver, whereas lactate dehydrogenase activity of muscle and acetylcholine esterase activity in brain were inhibited in cypermethrin-exposed fish. The membrane transport enzymes (total adenosine triphosphatase, sodium-potassium adenosine triphosphatase and magnesium adenosine triphosphatase) activities were decreased significantly in the gills of C. catla exposed to sub-lethal concentration of cypermethrin. The present study indicates that sub-lethal exposure of C. catla fingerlings to cypermethrin alters the haematological and biochemical parameters. © 2012 Springer Science+Business Media B.V. Source

Ranjan R.,VRC of CMFRI | Xavier B.,VRC of CMFRI | Dash B.,VRC of CMFRI | Edward L.L.,VRC of CMFRI | And 2 more authors.
Aquaculture Research

This study aimed to test the efficacy of 17 α-methyl testosterone (17 α-MT) alone and in combination with letrozole, an aromatase inhibitor, for the induction of sex reversal in protogynous greasy grouper, Epinephelus tauvina. Further, the long-lasting effects of these treatments and spawning performance of sex-reversed males were also investigated. Greasy grouper with oocytes in the perinucleolus stage were implanted with 5 mg 17 α-MT kg-1 body weight (T1), 5 mg 17 α-MT and 0.2 mg letrozole kg-1 body weight (T2) and 5 mg 17 α-MT with 0.4 mg letrozole kg-1 body weight (T3) and no androgens/enzyme inhibitor implanted (C). The 17 α-MT alone and in combination of letrozole-induced sex reversal in greasy grouper, whereas untreated control fish (C) showed normal ovarian development. However, T2 and T3 group showed 100% sex reversal and completion of spermatogenesis up to functional male phase in 2 and 3 months, respectively, whereas T1 group resulted in only 66.67% functional male with motile spermatozoa after 4 months. Sex-reversed males successfully fertilized the eggs during induced spawning. There were significant differences on fertilization and hatching rates between T2 group (79.00 ± 4.36%; 77.67 ± 2.87%, respectively) and T1 group (57.67 ± 3.17%; 63.87 ± 2.91%, respectively). The result suggested that 17 α-MT (5.0 mg kg-1 BW) in combination with letrozole (0.2 mg kg-1 BW) has the potential to produce 100% sex-reversed male in short period in greasy grouper, which might greatly help in seed production of greasy grouper. © 2015 John Wiley & Sons Ltd. Source

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