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Shuvaeva G.P.,Voronezh State Technical University | Sysoeva M.G.,Voronezh State Agricultural University
Applied Biochemistry and Microbiology | Year: 2010

Procedures for the production of endo-1,4-β-xylanase have been developed. An active producer-Rhizopus var. microsporus BKMF-595-has been chosen, and the conditions of surface and submerged cultivation, as well as the composition of the culture medium for this strain, have been optimized to ensure maximum yield of the target enzyme. Activity of xylomicrosporin Px equaled 123 U/g, while the activity of xylomicrosporin Gx equaled 25 U/cm3. Homogeneous enzyme preparations, purified 59.44-fold and 72.6-fold, have been obtained. The dependence of endo-1,4-β-xylanase catalytic activity on temperature and pH of the reaction medium has been studied. The enzyme has been shown to be most stable in the pH range 5.0-6.0 and to be thermostable. Amino acid composition and subunit structure of the enzyme were determined; the molecular masses of the subunits equaled 50 and 56 kDa. Carboxyl groups of glutamic and aspartic acid residues of the active center were experimentally shown to play an important role in catalysis. The potential of this enzyme for beer production has been demonstrated. © 2010 Pleiades Publishing, Ltd. Source

Lipatov D.N.,Moscow State University | Shcheglov A.I.,Moscow State University | Manakhov D.V.,Moscow State University | Zavgorodnyaya Y.A.,Moscow State University | Brekhov P.T.,Voronezh State Agricultural University
Eurasian Soil Science | Year: 2015

The spatial variability of the density, moisture, pH, humus, and benzo[a]pyrene contamination in a structural-metamorphic agrozem within the impact zone of the Yuzhno-Sakhalinsk thermal power plant has been considered. The correlation of the benzo[a]pyrene content with the humus and density in the plow horizon has been revealed. The necessary numbers of sampling points for different problems of ecological monitoring have been planned. © 2015, Pleiades Publishing, Ltd. Source

Voronezh State Agricultural University | Entity website

Home page Dear colleagues! Welcome to the home page of the official website of the theoretical and scientific journal Vestnik of VSAU. The purpose of the journal is to extend the knowledge of contemporary scientific research, advanced experience and know-how and other information in the field of agricultural production ...

Voronezh State Agricultural University | Entity website

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Voronezh State Agricultural University | Entity website

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