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Gorlov I.F.,Volgograd State Technical University | Kolosov Y.A.,Volgograd State Technical University | Shirokova N.V.,Volga Region Research Institute of Manufacture and Processing of Meat and Milk Production | Getmantseva L.V.,Don State Agrarian University | And 6 more authors.
Small Ruminant Research | Year: 2017

The aim of the study was to identify the GH/HaeIII gene polymorphism and to determine its association with growth traits in Salsk sheep bred in the Southern region of the Russian Federation. The identification of the GH gene polymorphism was performed by the PCR-RFLP method using the endonuclease HaeIII for restriction of an amplified fragment. During the experiment, the AA, AB, and BB genotypes of the Salsk breed sheep were identified at a frequency of 57, 36, and 7%, respectively. The values of the weight at weaning, at the age of 9 months and the average daily gain of the ram lamb with the AB genotype exceeded the values of the these parameters of the ram lamb with the AA genotype by 0.92 kg, 10.67 kg, and 47.3 g, respectively. The carcass weight, the weight of meat, the slaughter weight, and the slaughter yield of the ram lamb with the AB/GH genotype were found to be more as compared with the parameters of the ram lamb with the AA/GH genotype by 4.97 kg, 1.83 kg, 4.83 kg, and 2.04%, respectively. The ram's AB genotype also caused a greater weight of heart and kidney by 75.21 and 75.44 g, respectively. Thus, the presence of a heterozygous AB genotype in Salsk sheep breed has a positive effect on the growth traits. The rams of the AB/GH genotype significantly exceeded the rams of the AA/GH genotype and were found to have the best meat productivity. © 2017 Elsevier B.V.


Gorlov I.F.,Volgograd State Technical University | Khramtsov A.G.,North Caucasus Federal University | Evdokimov I.A.,North Caucasus Federal University | Lodygin A.D.,North Caucasus Federal University | And 5 more authors.
Pakistan Journal of Nutrition | Year: 2015

The purpose of the research was to explore the feasibility of enzymatic catalysis with elements of microbial synthesis of the polymerization of lactose monomers into the galactooligosaccharides (GalOS) nanoclusters. The main objects of the study were the lactose solution (milk raw sugar) and the whey permeates subjected to electroactivation and fermentation by Lactokanestsin G20x and Ha-Lactase. To determine the carbohydrate composition of samples and finished products, the high-pressure liquid chromatography method was used. For the process monitoring, the freezing point depression method and the lactose transformation rate were applied. The directed and controlled types of synthesis of a universal agricultural bioecologic raw material of animal origin were examined in the context of solving a task of transglycosylation of the whey lactose into the GalOS. A biotechnology paradigm of the enzyme-catalyzed GalOS polymerization by [beta]-galactosidase in model solutions of milk sugar and industrial samples of the whey permeates was substantiated. The transglycosylation patterns with optimized parameters of the GalOS synthesis were established. The GalOS synthesis in the whey permeate had an optimum, which was observed at a temperature of (50±2)°C and a pH value of (6.4±0.2). On the basis of the research results, the Innovative Technology Platform (the priority was patented with know-how elements) of the GalOS-concentrates production was generated. The relevance of this work is due not only to the practical applicability of the GalOS-concentrates as prebiotic components and their biological value, but also to the need for the complex processing of lactose containing raw material. © Asian Network for Scientific Information, 2015.


Sulimova G.E.,Russian Academy of Sciences | Voronkova V.N.,Volga Region Research Institute of Manufacture and Processing of Meat and Milk Production | Perchun A.V.,Russian Academy of Sciences | Gorlov I.F.,Volgograd State Technical University | And 3 more authors.
Russian Journal of Genetics | Year: 2016

The gene pools of beef cattle breeds bred in Russia were characterized on the basis of inter simple sequence repeat DNA analysis (ISSR analysis). Samples of Aberdeen Angus, Kalmyk, and Kazakh Whitehead breeds from Russia, as well as of Hereford breed, hybrids of Kazakh Whitehead and Hereford breeds, and Kazakh Whitehead breed from the Republic of Kazakhstan, were examined. In the examined breeds, 27 AG-ISSR fragments were identified, 25 of which were polymorphic. The examined breeds were different both in the fragment profiles (the presence/absence of individual ISSR fragments) and in their frequencies. It was demonstrated that the hybrid animals lacked some ISSR fragments that were present with high frequencies in parental forms, suggesting considerable genome rearrangement in the hybrid animals (at the regions of microsatellite localization) in crossings of the individuals from different breeds. The level of genetic diversity in Russian beef breeds was consistent with the values typical of farmed populations (breeds). The genetic diversity parameters assessed by applying Nei’s gene diversity index and the Shannon index varied from 0.0218 to 0.0605 and from 0.0225 to 0.0819, respectively. The highest Shannon index value was detected in the Kalmyk breed (0.0837) and Kazakh Whitehead breed from Russia (0.0819), and the highest level of Nei’s gene diversity index was found in the Kalmyk breed (0.0562) and in both populations of the Kazakh Whitehead breed (0.0509 and 0.0605). The high level of genetic similarity (according to Nei) was revealed between Russian beef cattle breeds and Hereford cattle: 0.839 (for the Kazakh Whitehead breed from Russia) and 0.769 (for the Kalmyk breed). © 2016, Pleiades Publishing, Inc.


Gorlov I.F.,Volga Region Research Institute of Manufacture and Processing of Meat and Milk Production | Gelunova O.B.,Volgograd State Technical University | Giro T.M.,Volgograd State Technical University | Mirzayanova E.P.,Saratov State Agrarian University
American Journal of Agricultural and Biological Science | Year: 2014

Integrated researches aimed to develop technologies and recipes for meat products of “Halal” category using seed fat and Milk Protein-Carbohydrate Concentrate (MPCC) represent a new generation of scientific and practical interest. In this connection, the authors propose technologies and recipes for meat products of “Halal” category, designed on the basis of rational combinatorics of animal and vegetable origin raw materials, that let us to expand the range of halal meat products and to develop cost-effective technologies. The results of the research have provided expanding the range of functional meat products, including Halal products for muslims, that meet the quality standards of the finished product in the category «Halal». Practical feasibility of using a vegetable fats complex and Milk Protein-Carbohydrate Concentrate (MPCC) «Lactobel-ED» in sausage production has been found. The levels of adding to the formula have been proved. Thermodenaturation of milk and whey proteins during thickening and drying of “Lactobel-ED” increases the availability of peptide chains and ionized amino acid residues. Solubility study showed that the concentrate is highly soluble in aqueous solutions, but this has a high dispersion degree, which increases the overall adsorption surface. Analysis of the results of structural and mechanical changes in the characteristics of minced model systems indicates a decline in YV of prototypes with sunflower oil on average 99.5 Pa. © 2014 I.F. Gorlov et al.


Gorlov I.F.,Volgograd State Technical University | Komarova Z.B.,Volga Region Research Institute of Manufacture and Processing of Meat And Milk Production | Nozhnik D.N.,Group of Companies Megamix | Zlobina E.Y.,Volga Region Research Institute of Manufacture and Processing of Meat And Milk Production | Karpenko E.V.,Volga Region Research Institute of Manufacture and Processing of Meat And Milk Production
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2016

Scientifically proves and experimentally confirms high efficiency of a micro element complex (OMEC) on the basis of L-aspartic amino acid containing iron, zinc, copper, and manganese in organic form in Cobb500 broiler chickens diet and its impact on productivity, consumption, digestibility, and utilization of diet nutrients by broiler chickens. Nutritional intervention with L-aspartates of the trace minerals (OMEC) to the Cobb500 broiler chickens diet contributed to a significant increase of the digestibility of essential nutrients in the feed. The effect of the micronutrient complex on the trace mineral content in bones, pectorals, and droppings has been found out. The use of manganese, iron, copper, and zinc L-aspartates in feeding Cobb500 broiler chickens allows to reduce this micronutrients content in the premix due to its high bioavailability and provides the necessary deposition of the trace elements in the backbone of broilers. The efficiency of organic minerals in feeding Cobb500 broiler chickens has been proved, since they improve the assimilation of zinc, copper, iron, and manganese, normalize these trace elements more accurately, and maintain the health of animals, their productive and reproductive qualities. Furthermore, organic minerals can significantly reduce the environmental pollution due to the decrease of their concentration in the droppings.


Gorlov I.F.,Volgograd State Technical University | Levakhin V.I.,All Russian Research Institute of Beef Cattle | Azhmuldinov E.A.,All Russian Research Institute of Beef Cattle | Levakhin G.I.,Orenburg State Agrarian University | And 5 more authors.
Asian Journal of Animal Sciences | Year: 2015

The purpose of this research was to study the possibility of cattle breeding in a radioactively contaminated area and to develop methods of purifying the animal body from cesium-137 before slaughter to obtain environmentally safe meat. Experiments were conducted on 4 groups of calves of Simmental breed grown with the cows and after weaning in a feedlot or in a fattening complex. The density of radioactive contamination by cesium-137 in the core sector was up to 925 kBq/m2, out at grass 851-1110 kBq/m2, in the "Clean" area, where the fattening complex was located to 185 kBq/m2. For the period of cleaning the calves-body with clean feeds, the decrease multiplicity of the cesium-137 content in comparison with the control was 17.0-33.7 times, respectively. Similar results were obtained in the study of animal internal organs (heart, liver, lungs and kidneys). The meat production was safe for use in human food. © 2016 Knowledgia Review, Malaysia.


Gorlov I.F.,Volgograd State Technical University | Giro T.M.,Saratov State Agrarian University | Sitnikova O.I.,Saratov State Agrarian University | Slozhenkina M.I.,Volgograd State Technical University | And 2 more authors.
American Journal of Food Technology | Year: 2016

Objective: The purpose of this study was to develop a technology of meat-vegetable pates based on mutton and poultry byproducts with addition of chickpeas for a functional nutrition of people who are prone to IDA or suffering from it. Methodology: The computer simulation helped to design formulations that meet the formalized Science-Based Recommendations (SBR), the nutritional adequacy of the virtual models of the pates was assessed. Results: Based on a comprehensive study of dynamics of water activity, pH and water-binding capacity, depending on the steeping mode, the technology for functional food pates has been worked out. The nutritional adequacy and the appropriate quality indicators of the meat-vegetable pates developed according to the SBR was defined. The medical and biological assessment of the meat-vegetable pates based on mutton and chicken by-products with the addition of a plant component distinguished by a balanced amino acid and fatty acid composition, a high animal protein content, macro and microelements, an optimal ratio of vitamin complex was carried out. The content of iron is 90 mg kg− 1, zinc 181.3 mg kg− 1, copper 17.1 mg kg− 1, selenium 0.093 mg kg− 1. Considering the recommended daily human consumption patterns of these trace elements, 100 g of the product developed satisfies the daily requirement of iron for 50-70%, zinc for 90%, copper 66%, selenium 15-20% that determines its functional healthy properties in the diet of patients with iron deficiency anemia. Conclusion: It has been found in vivo that the trace element composition of these products has a higher bioavailability, allowing to start the bodily compensatory reaction to the hematopoiesis dysfunction of iron deficiency character quickly. © 2016 Ivan Fiodorovich Gorlov et al.


Gorlov I.F.,Volgograd State Technical University | Slozhenkina M.I.,Volgograd State Technical University | Karpenko E.V.,Volga Region Research Institute of Manufacture and Processing of Meat and Milk Production | Giro T.M.,Saratov State Agrarian University | Andreeva S.V.,Saratov State Agrarian University
Voprosy Pitaniia | Year: 2015

The paper presents the biomedical evaluation of meat and cereal spread from low-cholesterol raw material with vegetable ingredients, recommended as a functional food. The experimental model with myocardial infarction like changes in hearts of the animals, accompanied by vascular changes similar to atherosclerotic changes in humans, as well as the modeling of the metabolic imbalance of lipids have been carried out by intramuscular injection of epinephrine and unbalanced feeding the animals with food rich in cholesterol, with a high content of carbohydrates and fats. Wistar rats were divided into 4 groups of 12 animals each. The rats in groups 1-3 were induced the cardio distress with intramuscular injection of epinephrine; group IV consisted of intact (healthy) animals. Dramatic changes in biochemical blood status that indicated heart disease have been observed within 2 days after the injection of epinephrine (0,2 mlper 1 kg of animal body weight) to the tested animals. During the experiment a sharp increase in activity of indicator enzymes of alanine aminotransferase (ALT) and aspartate aminotransferases (AST), with a predominance of AST over ALT, along with an increase in LDH activity have been observed. The 1,4-1,6 fold increase in blood serum creatinine has also been found. Later the animals in groups 1,2, 3 with simulated cardio pathology were fed a ration with intervention of food rich in cholesterol, with a high content of carbohydrates and fats (50% of the diet) for a month for induction of lipid metabolism disorders. An increase in the concentration of cholesterol and triglycerides by 3 fold or more has been observed. In addition, an accumulation of sulfhydryl groups has been noted, as evidenced by increased rates of thymol. For further normalization of lipid metabolism, the animals in tested group I were fed the diet with intervention of spread, developed in accordance with GOST 12318-91 Canned meat «Meat spread»; the rats of group 2 were fed with spread with vegetable supplements (pumpkin by-products and alginates), and group 3 received a standard diet within the next month. The results of biochemical blood serum studies have shown that the level of cholesterol, triglycerides, creatinine and sulphydryl groups in the experimental groups 1 and 2 decreased; indicating a positive influence on lipid metabolism and prevention of peroxides formation in the organism. These changes were more pronounced when meat product with plant additives and alginate was used in the diet. Only under the application of the meat-vegetable pate cholesterol level ceased to differ from that of the control group of animals. Based on the significant reduction of blood malondialdehyde level, antioxidant properties of vegetable supplements have been proven. The experimental data showed that the spread, developed on the basis of low-cholesterol raw meat with vegetable ingredients (pumpkin by-products and alginates) can be used to correct metabolic disorders of lipids, reducing the risk of cardio pathology in the body.

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