VNS Institute of Pharmacy

Bhopal, India

VNS Institute of Pharmacy

Bhopal, India

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Gupta S.P.,Shri Venkateshwara University | Garg G.,VNS Institute of Pharmacy
International Journal of Pharmacognosy and Phytochemical Research | Year: 2014

A simple and reproducible UV- spectrophotometric method for the quantitative determination of Tannic acid in Bhuvnesvara vati (BV) were developed and validated in the present work. The parameters, precision, accuracy, Limit of Detection and Limit of Quantitation were studied. In this present study a new, simple, rapid, sensitive, precise and economic spectrophotometric method in ultraviolet region has been developed for the determination of Tannic acid in laboratory ayurvedic formulation of Bhuvnesvara vati (BV). Each ingredient was purchased from the local market and identified morphologically and microscopically and compared with standard pharmacopoeial monograph. The concentration of tannic acid present in raw material is found to be 6.1%±0.27w/w in Emblica officinalis, 8.7%±0.31w/w in Terminalia belerica, 14.05%±0.29w/w in Terminalia chebula, 4.8%±0.94 in Aegle marmelus and 0.67±0.49 in Trichyspermum ammi and in three identical laboratory batch of Bhuvneshavara vati BV-I, BV-II and BV-III 4.90%±0.42, 4.79%±0.86, 4.85%±0.75w/w respectively with mean value 4.85%±0.53 w/w. Tannic acid has the maximum wavelength at 276 nm and hence the UV spectrophotometric method was performed at 276 nm. Tannic acid was found to follow beer lambert's law in concentration range 2-20μg/ml. The mean of %RSD value was found to be 0.399 with the mean standard error were 0.275.The content of Tannic acid in ayurvedic formulation was determined. The results of analysis have been validated statistically and confirmed the accuracy of the proposed method. Hence the proposed method can be used for the reliable quantification of Tannic acid in herbal formulations.


Rajak H.,Guru Ghasidas University | Singour P.,VNS Institute of Pharmacy | Kharya M.D.,Dr Hari Singh Gour University | Mishra P.,GLA Institute of Pharmaceutical science and Research
Chemical Biology and Drug Design | Year: 2011

In search for a better anticonvulsant drug and the importance of semicarbazones and 2,5-disubstituted 1,3,4-oxadiazoles as anticonvulsant pharmacophore, a series of novel substituted semicarbazones were designed, synthesized, and evaluated for their anticonvulsant activity. The chemical structures of the synthesized molecules were confirmed by elemental and spectral (IR, 1H NMR, 13C NMR and MS) analysis. The anticonvulsant activities of the compounds were investigated using maximal electroshock seizure and subcutaneous pentylenetetrazole (scPTZ) models. Efforts were also made to establish structure-activity relationships among synthesized compounds. The results of the present study validated that the pharmacophore model with four binding sites is essential for anticonvulsant activity. © 2011 John Wiley & Sons A/S.


Smita J.,VNS Institute of Pharmacy | Patil U.K.,Dr Hari Singh Gour University
Chinese Journal of Natural Medicines | Year: 2014

Aim: To investigate the active chloroform fraction of the ethanol extract of Ipomoea carnea flowers on hematological changes in toluene diisocyanate-induced inflammation in Wistar rats. Method: Except for the control group, all of the rats were sensitized with intranasal application of 5 μL of 10% toluene diisocyanate (TDI) for 7 days. One week after second sensitization, all of the rats were provoked with 5 μL of 5% TDI to induce airway hypersensitivity. After the last challenge, blood and bronchoalvelor lavage (BAL) fluid were collected and subjected to total and differential leucocytes count. Flash chromatography was performed on the most active chloroform fraction to isolate an individual component. Results: Treatment with the ethanolic extract and its chloroform fraction at an oral dose of 200 mg·kg-1 showed a significant decrease in circulating neutrophil and eosinophil in blood and BAL as compared with standard dexamethasone (DEXA). The structure of the compound obtained from chloroform fraction of Ipomea carnea was elucidated as stigmast-5, 22-dien-3β-ol on the basis of spectral data analysis. Conclusion: The chloroform fraction was found to be more effective to suppress airway hyper reactivity symptoms, and decreased count of both total and differential inflammatory cells. © 2014 China Pharmaceutical University.


Pawar R.S.,VNS Institute of Pharmacy | Jain A.P.,Dr Hari Singh Gour University | Lodhi S.,Dr Hari Singh Gour University | Singhai A.K.,Dr Hari Singh Gour University
Journal of Ethnopharmacology | Year: 2010

Ethnopharmacological relevance: Asteracantha longifolia Nees. (Family-Acanthaceae) is a wild herb commonly used in traditional ayurvedic medicine as Kokilaaksha and the Unani drug as Talimakhana in India and Srilanka for various medicinal uses as aphrodisiac, tonic, sedative and blood diseases etc. Aim of the study: The aim of the current study was to validate and explore the folk use of Asteracantha longifolia Nees. (AL) (Leaf part) on pharmacological grounds using haloperidol induced iron deficiency anemia for the assessment of erythropoietic activity. Materials and methods: Determination of iron in plant extracts was carried out using spectrophotometric method. Plant extract was obtained from crude drugs using extraction with ethanol. In vivo study, haloperidol induced iron deficiency anemia model was used in experimental studies. Results: An administration of ethanolic extract of AL at the doses of 100. mg/kg and 200. mg/kg body weight, i.p., demonstrated a significant (P<0.05) increase in erythrocyte count, haemoglobin count, serum iron and serum protein etc. This effect may be due to the presence of iron (622 μg/50. mg) in extract estimated by spectrophotometric method. Conclusion: An ethanolic extract of AL effectively restored the hematological parameters, serum iron and serum protein and normalized the microcytic (smaller in size), anisocytosis (disturbed shape) and hypochromic RBCs. These observations could justify the inclusion of this plant in the management of iron deficiency anemia due the presence of iron and other constituents as flavonoids, terpenoids, steroids, lupeol and betulin. © 2010 Elsevier Ireland Ltd.


Iyer D.,VNS Institute of Pharmacy | Patil U.K.,Peoples Institute of Pharmacy
Journal of Complementary and Integrative Medicine | Year: 2011

The present study was undertaken to explore the antihyperlipidemic effect of ethanolic extract of seeds of Apium graveolens L. and its chloroform and aqueous basic fraction in olive oil induced hyperlipidemic rats. The antihyperlipidemic activity of Apium graveolens was compared with a standard drug Atrovastatin (50mg/kg). The study involved phytochemical screening and chromatographic studies of extract and fractions. The ethanolic extract and its fractions were administered orally at doses of 200 and 400 mg/kg body weight in rats. Olive oil (5ml/kg oral dose) was administered 30 min after treatment. Blood was collected by ocular puncture 2 and 4 h after olive oil treatment and centrifuged at 3000 rpm for 15-20 min. Serum samples were further subjected to biochemical analysis. The study dose dependently inhibited the total cholesterol (TC) triglycerides (TG), low density lipoproteins (LDL) level, and significantly increased high density lipoprotein (HDL) level. Phytochemical screening revealed the presence of terpenoid, tannin, alkaloid, glycoside, flavanoid and sterols. UV λmax was found to be 206 nm with a melting point of 137-138°C for the isolated component. The antihyperlipidemic effect was evaluated in olive oil loaded rats. Acute treatment caused stimulatory effect on HDL level and inhibition in TC and TG elevation induced by olive oil. © 2011 Berkeley Electronic Press. All rights reserved.


Iyer D.,VNS Institute of Pharmacy | Sharma B.K.,VNS Institute of Pharmacy | Patil U.K.,Peoples Institute of Pharmacy
Pharmaceutical Biology | Year: 2011

Context: The papaya is the fruit of the plant Carica papaya L. (Caricaceae) used in India. Fruit and latex are both rich in an enzyme called papain. It is used as a folk remedy for contraception and abortion. Objective: The present study explored the anti-hyperlipidemic effect of the ether- and water-soluble fractions of C. papaya ethanol extract in olive oil-induced hyperlipidemic rats. The study also involved chromatographic studies of extract and fractions. Materials and methods: Flash chromatography was done for the most active fraction. The extract and fractions were administered orally at doses of 200 and 400mg/kg body weight in rats. Olive oil (5mL/kg oral dose) was administered 30min after treatment. Blood was collected and centrifuged at 3000rpm for 1520min and subjected to biochemical analysis. Result: The study dose-dependently inhibited the total cholesterol (TC), triglycerides (TG), low-density lipoproteins (LDL) level, and significantly increased high-density lipoprotein (HDL) level. Phytochemical screening revealed the presence of fats in the ether fraction, whereas the water fraction revealed the presence of tannins, alkaloids, glycosides. UV λmax was found to be 217nm with a melting point of 41°C for the isolated component. Discussion and conclusion: The anti-hyperlipidemic effect was evaluated in olive oil-loaded rats. Acute treatment caused stimulatory effect on HDL level and inhibition in TC and TG elevation induced by olive oil. The extract and water fraction showed protective action by increasing the HDL cholesterol level. © 2011 Informa Healthcare USA, Inc.


Chouksey D.,VNS Institute of Pharmacy | Upmanyu N.,P.A. College | Pawar R.S.,VNS Institute of Pharmacy
Asian Pacific Journal of Tropical Medicine | Year: 2013

Objective: To research the acute toxicity of Illicium verum (I. verum) fruit extracts and its action on central nervous system. Methods: The TLC and HPTLC techniques were used as fingerprints to determine the chemical components present in I. verum. Male albino rats and mice were utilized for study. The powdered material was successively extracted with n-hexane, ethyl acetate and methanol using a Soxhlet extractor. Acute toxicity studies were performed as per OECD guidelines. The CNS activity was evaluated on parameters of general behavior, sleeping pattern, locomotor activity, anxiety and myocoordination activity. The animals were trained for seven days prior to experiments and the divided into five groups with six animals in each. The drug was administered by intraperitoneal route according to body weight. The dosing was done as prescribed in each protocol. Results: Toxicity studies reported 2 000 mg/kg as toxicological dose and 1/10 of the same dose was taken as therapeutic dose Intraperitoneal injection of all extracts at dose of 200 mg prolonged phenobarbitone induced sleeping time, produced alteration in general behavior pattern, reduced locomotor activity and produced anxiolytic effects but the extracts do not significantly alter muscles coordination activity. The three extracts of I. verum at the dose of 200 mg, methanol extract was found to produce more prominent effects, then hexane and ethylacetate extracts. Conclusions: The observation suggested that the extracts of I. verum possess potent CNS depressant action and anxiolytic effect without interfering with motor coordination. © 2013 Hainan Medical College.


Jain S.,VNS Institute of Pharmacy | Patil U.K.,Peoples Institute of Pharmacy and Research Center
Indian Journal of Natural Products and Resources | Year: 2010

Cassia tora Linn. is one of the well-known anthraquinone containing plant and has been used in Chinese and Ayurvedic medicine. In the present review, an attempt has been made to explore a literature survey on its traditional uses, phytochemical studies and pharmacological properties. The whole plant as well as specific parts such as roots, leaves and seeds have been widely used and claimed against different diseases by rural and traditional practitioners of Satpura region of Madhya Pradesh. This plant has great contribution in modern system of herbal medicine for new drug development.


Soni M.,VNS Institute of Pharmacy | Mohanty P.K.,VNS Institute of Pharmacy | Jaliwala Y.A.,VNS Institute of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2011

Extract of Methanol:ethanol (70:30) of Prunus domestica was prepared and tested for its hepatoprotective effect against Paracetamol and CCl 4 induced hepatitis in rats. Alteration in the levels of biochemical markers of hepatic damage like SGPT, SGOT, ALP, Total bilirubin, direct bilirubin and tissue LPO, GSH, catalase and SOD were tested in both treated and untreated groups. Paracetamol (2 g/kg) and CCl 4 (1.5ml/kg) has enhanced the SGPT, SGOT, ALP, Total bilirubin and direct bilirubin and tissue level of GSH. Treatment with extract of P. domestica fruits (150 mg/kg and 300 mg/kg) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner.


Iyer D.,VNS Institute of Pharmacy | Patil U.K.,Peoples Institute of Pharmacy and Research Center
Asian Pacific Journal of Tropical Disease | Year: 2012

Objective: To explore the Anti-hyperlipidemic and Anti-tumor effect of ethanolic extract, chloroform fraction and stigmast-5-en-3β-ol from stems of Salvadora persica L. Methods: The antihyperlipidemic effect of ethanolic extract of Salvadora persica, its chloroform fraction and isolated components was evaluated in Triton- induced hyperlipidemic rats. Animals were administered with i.p. injection of Triton WR 1339 at dose of 400 mg/kg body weight. After 24 h. of Triton administration the test drugs were administered orally at doses of 200 mg/kg body weight in rats. The ethanolic extract and stigmast-5-en-3β-ol from Salvadora persica were further investigated for the tumor take inhibitory activity in hybrid mice (of C57BL strain + Swiss albino strain). Preventive group animals were injected daily with the extract and Stigmast-5-en-3β-ol at dose of 50mg/kg body weight i.p. for 10 consecutive days. The animals were observed for the growth of tumor after injection of B16F10 melanoma cells into the dorsal skin of mice. Results: Phytochemical investigation of stems of Salvadora persica resulted in the isolation of stigmast-5-en-3β-ol. The structure of the component has been established on the basis of spectral data analysis. UV λ max was found to be 206 nm with a melting point of 138°C for the isolated component. The stigmast-5-en-3β-ol showed inhibition of total cholesterol, triglycerides, low density lipoproteins level (LDL), and significantly increased high density lipoprotein level (HDL) in Triton- induced hyperlipidemic rats. It suggested marked antihyperlipidemic activity of stigmast-5-en-3β-ol at the oral dose of 200mg/kg. Pretreatment with the drug showed delay tumor growth by increasing the Volume Doubling Time (VDT) and Growth Delay (GD). The Stigmast-5-en-3β-ol had shown better mean survival time. Conclusions: These observations enabled to conclude that supplementation of antioxidants and phytosterols rich food exerts significant antihyperlipidemic and antitumor activity. The present investigation may be quite useful as this drug is highly valued as traditional system of medicine. © 2012 Asian Pacific Tropical Medicine Press.

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