VNS Institute of Pharmacy

Bhopal, India

VNS Institute of Pharmacy

Bhopal, India
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Gupta S.P.,Shri Venkateshwara University | Garg G.,VNS Institute of Pharmacy
International Journal of Pharmacognosy and Phytochemical Research | Year: 2014

A simple and reproducible UV- spectrophotometric method for the quantitative determination of Tannic acid in Bhuvnesvara vati (BV) were developed and validated in the present work. The parameters, precision, accuracy, Limit of Detection and Limit of Quantitation were studied. In this present study a new, simple, rapid, sensitive, precise and economic spectrophotometric method in ultraviolet region has been developed for the determination of Tannic acid in laboratory ayurvedic formulation of Bhuvnesvara vati (BV). Each ingredient was purchased from the local market and identified morphologically and microscopically and compared with standard pharmacopoeial monograph. The concentration of tannic acid present in raw material is found to be 6.1%±0.27w/w in Emblica officinalis, 8.7%±0.31w/w in Terminalia belerica, 14.05%±0.29w/w in Terminalia chebula, 4.8%±0.94 in Aegle marmelus and 0.67±0.49 in Trichyspermum ammi and in three identical laboratory batch of Bhuvneshavara vati BV-I, BV-II and BV-III 4.90%±0.42, 4.79%±0.86, 4.85%±0.75w/w respectively with mean value 4.85%±0.53 w/w. Tannic acid has the maximum wavelength at 276 nm and hence the UV spectrophotometric method was performed at 276 nm. Tannic acid was found to follow beer lambert's law in concentration range 2-20μg/ml. The mean of %RSD value was found to be 0.399 with the mean standard error were 0.275.The content of Tannic acid in ayurvedic formulation was determined. The results of analysis have been validated statistically and confirmed the accuracy of the proposed method. Hence the proposed method can be used for the reliable quantification of Tannic acid in herbal formulations.


Sachan R.,VNS Institute of Pharmacy | Khatri K.,VNS Institute of Pharmacy | Kasture S.B.,Pinnacle Biomedical Research Institute
International Journal of PharmTech Research | Year: 2010

Oral route is the easiest and most convenient route for drug administration. Oral drug delivery systems being the most cost-effective and leads the worldwide drug delivery market. The major problem in oral drug formulations is low and erratic bioavailability, which mainly results from poor aqueous solubility. This may lead to high inter- and intra subject variability, lack of dose proportionality and therapeutic failure. It is estimated that 40% of active substances are poorly water soluble (water insoluble in nature). For the improvement of bio-availability of drugs with such properties presents one of the greatest challenges in drug formulations. Various technological strategies are reported in the literature including solid dispersions, cyclodextrines complex formation, or micronization, and different technologies of drug delivery systems. Including these approaches self-emulsifying drug delivery system (SEDDS) has gained more attention for enhancement of oral bio-availability with reduction in dose. SEDDS are isotropic mixtures of oil, surfactants, solvents and co-solvents/surfactants. The principal characteristic of these systems is their ability to form fine oil-in-water (o/w) emulsions or micro-emulsions upon mild agitation following dilution by an aqueous phase. For lipophilic drugs, which have dissolution rate-limited absorption, SEDDS may be a promising strategy to improve the rate and extent of oral absorption. This review article explains how self-emulsifying drug delivery systems can increase the solubility and bioavailability of poorly soluble drug.


Gupta S.P.,Rajiv Gandhi Institute of Pharmacy | Garg G.,VNS Institute of Pharmacy
Der Pharmacia Lettre | Year: 2014

Bhuvnesvara vati (BV) is an Ayurvedic formulation containing Emblica officinalis, Terminalia belerica, Terminalia chebula, Trichyspermum ammi, Aegle marmelus and two minerals namely rock salt and Soot as main ingredients. The study was aimed to develop finger printing methods for well-known Ayurvedic formulation. Three sample batch of BV were prepared in the laboratory and two different marketed formulations were procured from Ayurvedic medicine shop. A HPLC method was developed for the estimation of gallic acid and tannic acid in laboratory and marketed formulations. The concentration of gallic acid present in raw material is found to be 3.174±0.049% w/w in Emblica officinalis, 8.920±0.173% w/w in Terminalia belerica, 4.092±0.117% w/w in Terminalia chebula, 1.831±0.973% w/w in Aegle marmelus and 0.264±0.365% w/w in Trichyspermum ammi. Gallic acid content in three identical laboratory batch of Bhuvnesvara vati BV-I, BV-II and BV-III, was found to be 2.623±0.746%, 2.589±0.356% and 2.632±0.239% w/w respectively. Two marketed formulation of Bhuvnesvara vati M-I and M-II showed gallic acid concentration to be 2.019±0.872 % and 2.019±0.872 % w/w respectively. The concentration of tannic acid present in raw material was found to be 6.172%±0.365w/w in Emblica officinalis, 8.667%±0.0319w/w in Terminalia belerica, 13.956%±0.745w/w in Terminalia chebula, 4.789±0.983% w/w in Aegle marmelus and 0.668±1.002% w/w in Trichyspermum ammi respectively and in three identical laboratory batch of Bhuvnesvara vati BV-I, BV-II and BV-III, was found to be 2.623±0.746%, 2.589±0.356%, 2.632±0.239% w/w respectively. In order to obtain precision and accuracy, the recovery study was performed and result obtained with mean value 99.69% for gallic acid and 99.38% for tannic acid, which prove reproducibility of the result. This show significant precision of methods at 95% confidence level. The mean of % RSD value was found to be 0.357 for gallic acid & 0.353 for tannic acid. Results of statistical analysis shows that present HPLC method for determination of gallic acid and tannic acid is simple, precise, accurate and suitable for routine analysis of gallic acid and tannic acid in BV. The developed fingerprints can be used as a standard and gallic acid and tannic acid can be used as a possible marker compound for fingerprinting of BV.


Uprit S.,P.A. College | Kumar Sahu R.,Columbia Institute of Pharmacy | Roy A.,Columbia Institute of Pharmacy | Pare A.,VNS Institute of Pharmacy
Saudi Pharmaceutical Journal | Year: 2013

In the present work attempts have been made to prepare the nanostructured lipid carrier (NLC) gel, by using minoxidil, which is preferably used in case of Alopecia, i.e. baldness pattern as a effective drug. The nine different formulations of Minoxidil-NLC (NLC1-NLC9) were prepared using solid and liquid lipids with Cholesterol and Soya lecithin in different concentrations by the melt dispersion ultrasonication method. Properties of NLC1-NLC9 such as the particle size and its distribution, the scanning electron microscopy (SEM), the drug entrapment efficiency (EE), and the drug release behavior were investigated. The nanoparticulate dispersion was suitably gelled and characterized with respect to drug content, pH, spreadability, rheology, and in vitro release. Safety of the NLC-based gel was assessed using primary skin irritation studies. The formulated NLC3 was spherical in shape, with average particle size of 280. nm, zeta potential of -42.40. mV and entrapment efficiency of 86.09%. Differential Scanning Calorimeter (DSC) measurements revealed that imperfect crystallization occurred in the inner core of the NLC particles. The drug release behavior from the NLC displayed a biphasic drug release pattern with burst release at the initial stage followed by sustained release. These results indicated that the NLC3 is a suitable carrier of minoxidil with improved drug loading capacity and controlled drug release properties. It has been observed that NLC gel produces the gel with good consistency, homogeneity, spreadability and rheological behavior. The developed NLC-based gel showed faster onset and elicited prolonged activity up to 16. h. The present study concluded that the NLC-based gel containing minoxidil dissolved in a mixture of solid lipid and liquid lipid in the nanoparticulate form helped us to attain the objective of faster onset yet prolonged action as evident from in vitro release. © 2012.


Shivhare Y.,VNS Institute of Pharmacy | Singour P.K.,VNS Institute of Pharmacy | Patil U.K.,VNS Institute of Pharmacy | Pawar R.S.,VNS Institute of Pharmacy
Journal of Ethnopharmacology | Year: 2010

Aim of the study: The present study provides a scientific evaluation for the wound healing potential of methanolic (MeOH) extract of TDR fruits. Materials and methods: Excision and incision wounds were inflicted upon three groups of six rats each. Group I was assigned as control (ointment base), Group II was treated with standard silver sulfadiazine (0.01%) cream. Group III was treated with 5% MeOH extract ointment. The parameters observed were percentage of wound contraction, epithelialization period, hydroxyproline content, tensile strength including histopathological studies. Results: It was noted that the effect produced by the extract ointment showed significant (P<0.01) healing in both the wound models when compared with control group. All parameters such as wound contraction, epithelialization period, hydroxyproline content, tensile strength and histopathological studies showed significant changes when compared to control. Conclusion: The result shows that TDR extract ointment demonstrates wound healing potential in both excision and incision models. © 2009 Elsevier Ireland Ltd.


Smita J.,VNS Institute of Pharmacy | Patil U.K.,Dr Hari Singh Gour University
Chinese Journal of Natural Medicines | Year: 2014

Aim: To investigate the active chloroform fraction of the ethanol extract of Ipomoea carnea flowers on hematological changes in toluene diisocyanate-induced inflammation in Wistar rats. Method: Except for the control group, all of the rats were sensitized with intranasal application of 5 μL of 10% toluene diisocyanate (TDI) for 7 days. One week after second sensitization, all of the rats were provoked with 5 μL of 5% TDI to induce airway hypersensitivity. After the last challenge, blood and bronchoalvelor lavage (BAL) fluid were collected and subjected to total and differential leucocytes count. Flash chromatography was performed on the most active chloroform fraction to isolate an individual component. Results: Treatment with the ethanolic extract and its chloroform fraction at an oral dose of 200 mg·kg-1 showed a significant decrease in circulating neutrophil and eosinophil in blood and BAL as compared with standard dexamethasone (DEXA). The structure of the compound obtained from chloroform fraction of Ipomea carnea was elucidated as stigmast-5, 22-dien-3β-ol on the basis of spectral data analysis. Conclusion: The chloroform fraction was found to be more effective to suppress airway hyper reactivity symptoms, and decreased count of both total and differential inflammatory cells. © 2014 China Pharmaceutical University.


Iyer D.,VNS Institute of Pharmacy | Patil U.K.,Peoples Institute of Pharmacy
Journal of Complementary and Integrative Medicine | Year: 2011

The present study was undertaken to explore the antihyperlipidemic effect of ethanolic extract of seeds of Apium graveolens L. and its chloroform and aqueous basic fraction in olive oil induced hyperlipidemic rats. The antihyperlipidemic activity of Apium graveolens was compared with a standard drug Atrovastatin (50mg/kg). The study involved phytochemical screening and chromatographic studies of extract and fractions. The ethanolic extract and its fractions were administered orally at doses of 200 and 400 mg/kg body weight in rats. Olive oil (5ml/kg oral dose) was administered 30 min after treatment. Blood was collected by ocular puncture 2 and 4 h after olive oil treatment and centrifuged at 3000 rpm for 15-20 min. Serum samples were further subjected to biochemical analysis. The study dose dependently inhibited the total cholesterol (TC) triglycerides (TG), low density lipoproteins (LDL) level, and significantly increased high density lipoprotein (HDL) level. Phytochemical screening revealed the presence of terpenoid, tannin, alkaloid, glycoside, flavanoid and sterols. UV λmax was found to be 206 nm with a melting point of 137-138°C for the isolated component. The antihyperlipidemic effect was evaluated in olive oil loaded rats. Acute treatment caused stimulatory effect on HDL level and inhibition in TC and TG elevation induced by olive oil. © 2011 Berkeley Electronic Press. All rights reserved.


Jain S.,VNS Institute of Pharmacy | Patil U.K.,Peoples Institute of Pharmacy and Research Center
Indian Journal of Natural Products and Resources | Year: 2010

Cassia tora Linn. is one of the well-known anthraquinone containing plant and has been used in Chinese and Ayurvedic medicine. In the present review, an attempt has been made to explore a literature survey on its traditional uses, phytochemical studies and pharmacological properties. The whole plant as well as specific parts such as roots, leaves and seeds have been widely used and claimed against different diseases by rural and traditional practitioners of Satpura region of Madhya Pradesh. This plant has great contribution in modern system of herbal medicine for new drug development.


Soni M.,VNS Institute of Pharmacy | Mohanty P.K.,VNS Institute of Pharmacy | Jaliwala Y.A.,VNS Institute of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2011

Extract of Methanol:ethanol (70:30) of Prunus domestica was prepared and tested for its hepatoprotective effect against Paracetamol and CCl 4 induced hepatitis in rats. Alteration in the levels of biochemical markers of hepatic damage like SGPT, SGOT, ALP, Total bilirubin, direct bilirubin and tissue LPO, GSH, catalase and SOD were tested in both treated and untreated groups. Paracetamol (2 g/kg) and CCl 4 (1.5ml/kg) has enhanced the SGPT, SGOT, ALP, Total bilirubin and direct bilirubin and tissue level of GSH. Treatment with extract of P. domestica fruits (150 mg/kg and 300 mg/kg) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner.


Iyer D.,VNS Institute of Pharmacy | Patil U.K.,Peoples Institute of Pharmacy and Research Center
Asian Pacific Journal of Tropical Disease | Year: 2012

Objective: To explore the Anti-hyperlipidemic and Anti-tumor effect of ethanolic extract, chloroform fraction and stigmast-5-en-3β-ol from stems of Salvadora persica L. Methods: The antihyperlipidemic effect of ethanolic extract of Salvadora persica, its chloroform fraction and isolated components was evaluated in Triton- induced hyperlipidemic rats. Animals were administered with i.p. injection of Triton WR 1339 at dose of 400 mg/kg body weight. After 24 h. of Triton administration the test drugs were administered orally at doses of 200 mg/kg body weight in rats. The ethanolic extract and stigmast-5-en-3β-ol from Salvadora persica were further investigated for the tumor take inhibitory activity in hybrid mice (of C57BL strain + Swiss albino strain). Preventive group animals were injected daily with the extract and Stigmast-5-en-3β-ol at dose of 50mg/kg body weight i.p. for 10 consecutive days. The animals were observed for the growth of tumor after injection of B16F10 melanoma cells into the dorsal skin of mice. Results: Phytochemical investigation of stems of Salvadora persica resulted in the isolation of stigmast-5-en-3β-ol. The structure of the component has been established on the basis of spectral data analysis. UV λ max was found to be 206 nm with a melting point of 138°C for the isolated component. The stigmast-5-en-3β-ol showed inhibition of total cholesterol, triglycerides, low density lipoproteins level (LDL), and significantly increased high density lipoprotein level (HDL) in Triton- induced hyperlipidemic rats. It suggested marked antihyperlipidemic activity of stigmast-5-en-3β-ol at the oral dose of 200mg/kg. Pretreatment with the drug showed delay tumor growth by increasing the Volume Doubling Time (VDT) and Growth Delay (GD). The Stigmast-5-en-3β-ol had shown better mean survival time. Conclusions: These observations enabled to conclude that supplementation of antioxidants and phytosterols rich food exerts significant antihyperlipidemic and antitumor activity. The present investigation may be quite useful as this drug is highly valued as traditional system of medicine. © 2012 Asian Pacific Tropical Medicine Press.

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