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Srivastava A.K.,Sapience Bio analytical Research Laboratory | Singour P.K.,VNS Group of Institutions | Gouda P.,Khallikote Autonomous College
Central Nervous System Agents in Medicinal Chemistry | Year: 2016

Monoamine oxidases A and B (MAO-A and B) play a critical role in the metabolism of intracellular neurotransmitters of the central nervous system. For decades, MAO inhibitors have proven their clinical efficacy as potential drug targets for several neurological and neurodegenerative diseases. Use of first generation non selective MAO inhibitors as neuropsychiatric drugs elicited several side effects like hypertensive crisis and cheese reaction. Therefore their use is now limited due to non-selectivity towards MAO isoforms and inhibition of metabolizing enzymes like cytochrome P450. Development of selective and specific MAO inhibitors like moclobemide, toloxatone improves their efficacy as disease-modifying effects in monotherapy as well as adjunctive therapy. Recently a lot of research has been done to elucidate the pharmacological potential of medicinal plants and their isolated bioactive constituents having MAO inhibitory activity. Herbs containing MAO inhibitors are extensively used for the development of potent synthetic drugs and as safe and effective alternatives to the available synthetic drugs in the treatment of neurodegenerative diseases such as depression, Parkinson and Alzheimer’s. In several diseases like Parkinson natural MAO inhibitors prevented the neuron denaturalization by their dual action via enhancing neurotransmission of dopamine as well as lowering the generation of free radicals and toxins. Currently development of selective MAO inhibitors is still under study to achieve more effective therapies by using Computer Aided Drug Designing, Ligand-based models and structure-activity hypothesis. These approaches also facilitate understanding the interaction of newly designed molecule with MAO enzymes and the rationalization of probable mechanisms of action. © 2016 Bentham Science Publishers.


Pawar R.S.,VNS Group of Institutions | Chaurasiya P.K.,VNS Group of Institutions | Rajak H.,Guru Ghasidas University | Singour P.K.,VNS Group of Institutions | And 2 more authors.
Indian Journal of Pharmacology | Year: 2013

Introduction: The present study provides a scientific evaluation for the wound healing potential of ethanolic (EtOH) extract of Sida cordifolia Linn. (SCL) plant. Materials and Methods: Excision, incision and burn wounds were inflicted upon three groups of six rats each. Group I was assigned as control (ointment base). Group II was treated with 10% EtOH extract ointment. Group III was treated with standard silver sulfadiazine (0.01%) cream. The parameters observed were percentage of wound contraction, epithelialization period, hydroxyproline content, tensile strength including histopathological studies. Result: It was noted that the effect produced by the ethanolic extract of SCL ointment showed significant (P < 0.01) healing in all wound models when compared with the control group. All parameters such as wound contraction, epithelialization period, hydroxyproline content, tensile strength and histopathological studies showed significant (P < 0.01) changes when compared with the control. Conclusion: The ethanolic extract ointment of SCL effectively stimulates wound contraction; increases tensile strength of excision, incision and burn wounds.


Purohit B.,VNS Group of Institutions | Gupta N.,VNS Group of Institutions | Jain S.,VNS Group of Institutions
Research Journal of Pharmacy and Technology | Year: 2013

The objective of present study was to develop diclofenac sodium organogel using sorbitan monoesters. Various organogels were prepared using isopropyl myristate, tween 80, sorbitan monostearate and sorbitan monopalmitate as organogelator. The formulated organogels were evaluated for their appearance, homogeneity, pH, viscosity, spreadibility, and % drug content and drug release. The drug release study was carried out using Franz diffusion cell.Rat skin of suitable size was clamped on to the cell. Organogel containing 7.5% of sorbitan monostearate showed highest drug release rate, however on further increasing the organogelator concentration the drug release rate was found to decrease. This decrease in drug release rate can be attributed to entrapment of drug into fiber network which hinder drug release. Organogel containing sorbitan monopalmiatate showed a lower drug release rate. © RJPT All right reserved.


PubMed | VNS Group of Institutions
Type: Journal Article | Journal: Nigerian journal of clinical practice | Year: 2016

The use of a simple and reproducible model is inevitable for an objective statement of the effects of external factors on wound healing. Hence, the present study was conducted to evaluate wound healing activities of sequential different extracts of Aegle marmelos leaves (AM) and Mucuna pruriens seeds (MP) by in vivo experimental models.Wistar albino rats were subjected to excision, incision and dead space wounds measuring approximately 250 mm2, 3 cm and implanting sterilized polyvinyl chloride tube on the back of each rat near either side of the vertebral column respectively. The experimental animals were randomized into eight groups (n = 6), control, standard and treatment groups. Hydrogel of different extracts were applied topically once daily. The parameters observed were percentage of wound contraction, epithelization period, tensile strength, hydroxyproline content of the granulation tissue, and histological changes during wound healing.The statistical study revealed that in excision, incision, and dead space wound models all formulations have significant (P < 0.01) wound healing potential. However, methanolic extract formulation was found to be superior to all other treatments as evidenced by rapid wound contraction, lesser number of days required for complete epithelization, increased tensile strength and significant increase in hydroxyproline content.As compared to the reference standard treated group the wound healing process of the experimental groups was decelerated. All extracts obtained from AM and MP facilitated the wound healing process in all experimental models.


Shivhare M.K.,VNS Group of Institutions | Singour P.K.,VNS Group of Institutions | Chaurasiya P.K.,VNS Group of Institutions | Pawar R.S.,VNS Group of Institutions
Pharmacognosy Reviews | Year: 2012

World Health Organization (WHO) has recommended that traditional health and folk medicine systems are proved to be more effective in health problems worldwide. Trianthema portulacastrum Linn. is a herb used in Ayurvedic medicine. The principal constituent of T. portulacastrum Linn. is ecdysterone and the other constituents are trianthenol, 3-acetylaleuritolic acid, 5,2′-dihydroxy-7-methoxy-6,8-dimethylflavone, leptorumol, 3,4-dimethoxy cinnamic acid, 5-hydroxy-2-methoxybenzaldehyde, p-methoxybenzoic acid, and beta cyanin. Different parts of Trianthema portulacastrum Linn. are traditionally used as analgesic, stomachic, laxative, treatment of blood disease, anemia, inflammation, and night blindness. Laboratory investigations on extracts of the plant have demonstrated significant pharmacological activities, such as antioxidant, diuretic, analgesic, hepatoprotective, and anticarcinogenic. This article compiles all updated information related to T. portulacastrum Linn. Scientifically proved activities are co-related with traditional concepts. Scientific evidence exists with respect to their major and minor constituents. The novelty and applicability of T. portulacastrum are hidden. Such things should be overcome through modern scientific concepts.


Swami P.D.,Samrat Ashok Technological Institute | Sharma R.,Samrat Ashok Technological Institute | Jain A.,Samrat Ashok Technological Institute | Swami D.K.,VNS Group of Institutions
Speech Communication | Year: 2015

Abstract This paper focuses on employing adaptive scales for computation of perceptually scaled continuous wavelet transform coefficients (CWT) and adaptive thresholding of these coefficients for speech enhancement. The adaptive scales and thresholds both were decided on the basis of the noise level of the noisy speech signal. The CWT coefficients were scaled perceptually and the proposed algorithm suggests selection of number of scales required for analysis on the basis of noise level. The CWT coefficients were then thresholded and for this a novel method of generating adaptive thresholds that too depends on the noise level of the noisy signal has also been proposed. Speech signals were acquired from the TIMIT database and evaluation of the proposed method is done by corrupting these signals by white Gaussian noise (at -10, -5, 0, 5, 10, 15 and 20 dB SNRs) and four real world noises (each at 0 dB SNR); pink, babble, car interior and F16 cockpit noise from the NOISEX-92 database. Enhancement results are compared on the basis of signal to noise ratio (SNR), segmental SNR (SSNR), spectral distortion (SD) and perceptual evaluation of speech quality (PESQ). Results of the proposed method are evaluated against Ephraim Malah filtering, Stein's unbiased risk estimate (SURE) thresholding of bionic wavelet transform (BWT) coefficients (BWT-SURE), Wiener filtering (WF), perceptually scaled wavelet packet transform (PWT), multi-model WF and multi-model sparse code shrinkage (MultiSCS) enhancement methods. For the white Gaussian noise case, at all noise levels, SNR and SSNR of the proposed method were better than all the methods under comparison. SD and PESQ results were lower than multiSCS method at 10 dB SNR but better at 15 dB and 20 dB SNRs. For the babble noise case, the obtained results were lower than Ephraim Malah but better than BWT-SURE. SNR and SSNR results for the cockpit noise were comparable with Ephraim Malah and BWT-SURE while for the pink noise case, the proposed method gives the best results. © 2015 Elsevier B.V. All rights reserved.


PubMed | VNS Group of Institutions
Type: Journal Article | Journal: Indian journal of pharmacology | Year: 2015

The aim of the study was to assess the curcumin containing ethanolic extract (EtOH) obtained from Curcuma longa (Cl) against retardation of wound healing by aspirin.Wound healing process was retarded by administering the dose of 150 mg/kg body weight of aspirin orally for 9 days to observe the effect of EtOH obtained from Cl using excision and incision wound model in rats. The various parameters such as % wound contraction, epithelialization period, hydroxyproline, tensile strength were observed at variant time intervals and histopathological study was also performed.Curcumin containing 5% and 10% ethanolic extract ointment have shown significant (P < 0.01) wound healing activity against an aspirin (administered 150 mg/kg body weight orally for 9 days) retarded wound healing process. Topical application of ointment showed significant (P < 0.01) difference as compared to the control group. Histopathological studies also showed healing of the epidermis, increased collagen, fibroblasts and blood vessels.Ethanolic extract of Cl ointment (EtOHCl) containing 10% curcumin displayed remarkable healing process against wound retardation by aspirin.


PubMed | VNS Group of Institutions
Type: Journal Article | Journal: Indian journal of pharmacology | Year: 2013

The present study provides a scientific evaluation for the wound healing potential of ethanolic (EtOH) extract of Sida cordifolia Linn. (SCL) plant.Excision, incision and burn wounds were inflicted upon three groups of six rats each. Group I was assigned as control (ointment base). Group II was treated with 10% EtOH extract ointment. Group III was treated with standard silver sulfadiazine (0.01%) cream. The parameters observed were percentage of wound contraction, epithelialization period, hydroxyproline content, tensile strength including histopathological studies.It was noted that the effect produced by the ethanolic extract of SCL ointment showed significant (P < 0.01) healing in all wound models when compared with the control group. All parameters such as wound contraction, epithelialization period, hydroxyproline content, tensile strength and histopathological studies showed significant (P < 0.01) changes when compared with the control.The ethanolic extract ointment of SCL effectively stimulates wound contraction; increases tensile strength of excision, incision and burn wounds.


PubMed | VNS Group of Institutions
Type: Journal Article | Journal: Pharmacognosy research | Year: 2014

Ajuga bracteosa Wall ex Benth (Labiatae) is popularly known in India as Neelkanthi. A decoction of the leaves, flowers, and barks is used in India for the treatment of cancer including diabetes, malaria, and inflammation etc. The main objective of this study is to investigate the cytotoxic potential of Ajuga bracteosa.Successive solvent extraction of Ajuga bracteosa in petroleum ether, methanol, and water extracts was done. These extracts were tested against human breast adenocarcinoma (MCF-7) and larynx carcinoma (Hep-2) tumor cell lines, using the thiazolyl blue test (MTT) assay.The methanolic fraction of Ajuga bracteosa had shown the significant results against MCF-7 and Hep-2 tumor cell lines. The methanolic, petroleum ether and aqueous extract from Ajuga bracteosa, presented an IC50 value at 24 h of 10, 65, 70 g/ml and 5, 30, 15 g/ml on MCF-7 and Hep-2 cells, respectively. Steroids compounds namely -sitosterol and unknown constituents were identified in the most active methanol extract of Ajuga bracteosa wall ex Benth. These known and unknown compounds exhibited cytotoxic potential against MCF-7 and Hep-2 cancer cells.Among all the tested extracts, methanolic extract can be considered as potential sources of anti-cancer compounds. Further studies are necessary for more extensive biological evaluations.


PubMed | VNS Group of Institutions
Type: Journal Article | Journal: Pharmacognosy reviews | Year: 2012

World Health Organization (WHO) has recommended that traditional health and folk medicine systems are proved to be more effective in health problems worldwide. Trianthema portulacastrum Linn. is a herb used in Ayurvedic medicine. The principal constituent of T. portulacastrum Linn. is ecdysterone and the other constituents are trianthenol, 3-acetylaleuritolic acid, 5,2-dihydroxy-7-methoxy-6,8-dimethylflavone, leptorumol, 3,4-dimethoxy cinnamic acid, 5-hydroxy-2-methoxybenzaldehyde, p-methoxybenzoic acid, and beta cyanin. Different parts of Trianthema portulacastrum Linn. are traditionally used as analgesic, stomachic, laxative, treatment of blood disease, anemia, inflammation, and night blindness. Laboratory investigations on extracts of the plant have demonstrated significant pharmacological activities, such as antioxidant, diuretic, analgesic, hepatoprotective, and anticarcinogenic. This article compiles all updated information related to T. portulacastrum Linn. Scientifically proved activities are co-related with traditional concepts. Scientific evidence exists with respect to their major and minor constituents. The novelty and applicability of T. portulacastrum are hidden. Such things should be overcome through modern scientific concepts.

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