Vladivostok State Medical University

Vladivostok, Russia

Pacific State Medical University , formerly known as VSMU is a university in Vladivostok in the Far East of Russia.At the beginning, since 1956 VSMU was the Medical Faculty of the Far Eastern State University, but in 2 years it became Vladivostok State Medical Institute . Institute was reorganized into the University in 1995, in 2013 the University was renamed and reorganized into Pacific State Medical University.Faculties of the Pasific State Medical University: The Medical Faculty The Military Faculty The Military Training Center The Pediatric Faculty The Faculty of Medical Prophylactic and Medical BiochemistryAmong the medical Universities of the Far East PSMU - the only institution that trains doctors at Faculty Medical Prophylactic , providing preventive measures for healthy living of the population of Khabarovsk, Primorsky and Kamchatka Territories, Amur, Sakhalin and Magadan regions, the Republic of Sakha and Chukotka. The Pharmaceutical Faculty The Stomatological Faculty The Higher Nursing Education and Social Work Faculty The Clinical Psychology Faculty The Continuous Medical Education The Postgraduate and Further Degree Studies Pre-University Training Wikipedia.

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Kotsyuba A.E.,Vladivostok State Medical University | Chertok V.M.,Vladivostok State Medical University
Neuroscience and Behavioral Physiology | Year: 2013

An immunocytochemical method was used to study the distribution of neurons expressing heme oxygenase-2 (HO-2) in the nuclei of different parts of the brainstem in 16 male Wistar rats. Neuron sizes and cytoplasmic histochemical reaction product optical densities were determined in various nuclei. The nuclei of the medulla oblongata, pons, and midbrain were found to contain HO-2-positive neurons with different shapes, numbers, and sizes. HO-2-positive neurons were found 3-5 times more frequently in sensory nuclei than in motor nuclei. However, there were relatively large numbers of both sensory and motor nuclei in which HO-2-positive neurons were not seen or were identified only as occasional cells. © 2013 Springer Science+Business Media New York.

Kabalyk M.A.,Vladivostok State Medical University
Nauchno-Prakticheskaya Revmatologiya | Year: 2017

Objective: to establish the specific features of changes in the levels of heat shock proteins (HSP), chemokines, and a marker for collagen degradation in the blood of patients with osteoarthritis (OA) depending on the stage of the disease. Subjects and methods. 99 patients with knee OA were examined in the rheumatology room, Vladivostok Polyclinic Three. The diagnosis was verified in accordance with the 2010 European League Against Rheumatism (EULAR) criteria. This group comprised 87 (88%) women and 12 (12%) men; the patients' mean age was 66.7±7.9 years; the disease duration was 5.9±4.0 years. A control group included 21 apparently healthy women and 9 men; their mean age was 59.6±8.3 years. Enzyme immunoassay was carried out to determine the concentrations of query molecules in the blood of the patients included in the investigation. The investigators used ELISA kits for HSP70, HSP27 (SunLong Biotech Co. Ltd, China), CRTAP (cartilage-associated protein, CAP), TNF-α (tumor necrosis factor-α), and CXCL17 (chemokine (C-X-C motif) ligand 17) (Cloud-Clone Corp., USA). Results and discussion. The levels of HSP27 and HSP70, their ratio, and CAP in OA were significantly lower than those in the control group. Those of TNF-α and CXCL17 were, on the contrary, considerably higher than those in the control group. In the patients with OA, HSP70 demonstrated an inverse correlation with the levels of CAP and TNF- α. CAP was statistically significantly correlated with TNF-α. The latter was directly related to CXCL17. When the duration of the disease was 10 or more years, the level of CAP was significantly higher than that in patients with a 5-9-year history of OA (p < 0.05) and did not differ from that in those with OA of 1-4-year duration (p > 0.05). The level of CXCL17 reduced statistically significantly with longer disease duration (p < 0.05). In a group of patients with a 1-4-year history of OA, the disease duration had a direct significant correlation with the levels of CAP and TNF-α. When the history of the disease was 5-9 years, there was a significant direct relationship of the duration of OA to CAP and CXCL17. In the patients who fell ill 10 or more years ago, the duration of the disease was directly correlated with the levels of CAP, TNF-α, and CXCL17.

Chertok V.M.,Vladivostok State Medical University | Kotsyuba A.E.,Vladivostok State Medical University
Neuroscience and Behavioral Physiology | Year: 2014

The distribution of nitroxidergic neurons in the medulla oblongata nuclei in Wistar rats (n = 8) was studied using histochemical (NADPH-diaphorase) and immunohistochemical methods with antisera to the neuronal form of nitric oxide synthase (nNOS). NADPH-diaphorase was found in large and small neurons in the sensory, autonomic, and motor nuclei. The latter were found to contain particularly large numbers of neurons expression this enzyme activity. In contrast to NADPH-diaphorase, nNOS in the corresponding nuclei was always present in smaller quantities of mainly smaller neurons. The sensory nuclei (solitary tract nucleus, reticular parvocellular and lateral nuclei, spinal nucleus of the trigeminal nerve) showed 1.5–3 times more nNOS neurons than the motor nuclei. In some nuclei (the nucleus ambiguus, the nucleus of the hypoglossal nerve) containing large numbers of NADPH-diaphorase-positive neurons, immunoreactive cells were particularly rare. © 2014, Springer Science+Business Media New York.

Van Loghem J.,Doctors Inc. | Yutskovskaya Y.A.,Vladivostok State Medical University | Werschler W.M.P.,University of Washington
Journal of Clinical and Aesthetic Dermatology | Year: 2015

Background: Calcium hydroxylapatite is one of the most well-studied dermal fillers worldwide and has been extensively used for the correction of moderate-to-severe facial lines and folds and to replenish lost volume. Objectives: To mark the milestone of 10 years of use in the aesthetic field, this review will consider the evolution of calcium hydroxylapatite in aesthetic medicine, provide a detailed injection protocol for a global facial approach, and examine how the unique properties of calcium hydroxylapatite provide it with an important place in today's market. Methods: This article is an up-to-date review of calcium hydroxylapatite in aesthetic medicine along with procedures for its use, including a detailed injection protocol for a global facial approach by three expert injectors. Conclusion: Calcium hydroxylapatite is a very effective agent for many areas of facial soft tissue augmentation and is associated with a high and well-established safety profile. Calcium hydroxylapatite combines high elasticity and viscosity with an ability to induce long-term collagen formation making it an ideal agent for a global facial approach.

Agafonova I.G.,RAS Pacific Institute of Bioorganic Chemistry | Kotel'nikov V.N.,Vladivostok State Medical University | Eichhoff U.,Bruker Biospin Gmbh
Applied Magnetic Resonance | Year: 2014

OXYS rats represent a selection strain of laboratory animals, which are characterized by the accelerated senescence. Substantial morphologic changes of cerebral vessels were revealed in the senescence-accelerated OXYS rats by the noninvasive MRI diagnostics using the induced arterial hypertension and the author's original methods. These changes appeared as vascular lesions with a thickening of the intimae and the increasing of the signal intensity from blood in cerebral vessels. Cerebral arterial hypertension in normotensive Wistar rats developed as result of intraperitoneal injections of hydrocortisone acetate and diet enriched by sodium ions. The pathology of cerebral vessels in OXYS rats began its development much earlier and was based on spontaneous hypertension in connection with initially elevated blood pressure as well as the genotype of the animals. Four different inductors (two vasodilators and two vasoconstrictors) were used in the study of the endothelium-independent and endothelium-dependent vasodilatation and vasoconstrictions. We have compared previously unknown effects of these inductors on cerebral vessels in senescence-accelerated OXYS rats and those in normal Wistar rats. The response of the arteries to the action of inductors showed changes in the status of anterior, middle, and posterior cerebral arteries of circle Willis. Thus, we have indicated the changes in compensatory and adaptive characters of arteries in hypertensive OXYS rats in comparison with hypertensive Wistar rats. Reduced vasodilator response in the middle cerebral arteries suggests an elevated risk for development of arterial hypertension in these rats. © 2014 Springer-Verlag Wien.

Dubikov A.I.,Vladivostok State Medical University | Kalinichenko S.G.,Vladivostok State Medical University
Scandinavian Journal of Rheumatology | Year: 2010

Objectives: To study the rate of apoptosis and expression of pro-and anti-apoptotic molecules in the synovial membrane in early and late rheumatoid arthritis (RA). Methods: Samples of the synovium, cartilage, synovial fluid, and blood serum were obtained from patients with seropositive RA. The localization of Bcl-2-, p53-and TUNEL-immunoreactive cells in the synovial membrane was studied. The level of the soluble Fas (sFas) receptor was determined in the blood serum and synovial fluid using an immunoassay. Results: In early RA, p53-immunoreactive synovial cells of type A were found to form rare aggregates in the intima. In late RA, on the contrary, these cells increased in number and occurred predominantly in the synovial stroma. Bcl-2-immunoreactive synovial cells were observed in lymphocytic infiltrates in the intima. They were found mainly in early RA. In late RA, their number decreased. The apoptotic index determined from the proportion of TUNEL-reactive synovial cell nuclei reached a maximum in late RA. The temporal differences in the rate of apoptosis were correlated with the humoral level of sFas, which increased significantly in late RA. On the contrary, in the synovial fluid, the sFas level decreased monotonically from early to late RA. Conclusion: In early RA, in the synovial membrane, the rate of apoptosis and p-53-immunostaining intensity were low, and Bcl-2-immunostaining intensity was high. The sFas level in synovial fluid was high. In late RA, the rate of apoptosis and p-53-immunostaining intensity increased, Bcl-2-immunostaining intensity decreased, as did the sFas level. © 2010 Taylor & Francis on license from Scandinavian Rheumatology Research Foundation.

Chertok V.M.,Vladivostok State Medical University | Kotsyuba A.E.,Vladivostok State Medical University
Bulletin of Experimental Biology and Medicine | Year: 2012

The distribution of two enzymes involved in H2S synthesis, cystationine β-synthase (CBS) and cystationine γ-liase (CSE), was studied in the walls of the internal carotid artery, order I-V branches of the middle cerebral artery basin, and intracerebral vessels of adult Wistar rats. Immunohistochemical staining showed the presence of CBS in the endothelium of small pial arteries (order IV-V branches) and intracerebral arterioles and in the capillary walls, neurons, and vascular nerves. As for CSE, in the internal carotid artery and large (order I-II) pial branches it was found mainly in the tunica media myocytes, in order III-IV vessels in myocytes and endothelium, and in smaller pial and intracerebral vessels in the endothelium. Along with enzyme-positive vessels, many pial and intracerebral arteries contained no these enzymes in the walls. © 2012 Springer Science+Business Media New York.

Kovalchuk V.K.,Vladivostok State Medical University
Human Ecology | Year: 2015

A hygienic estimation of the actual average daily dose (AADD) of iron, consumed by the adolescent population with the sources of its per oral administration in human bodies in the conditions of iron excess (0.8 mg/l) in tap water has been performed. An object of the study was a random sample of 156 schoolchildren aged 14-17 years from Vladivostok. Methods: All adolescents were interviewed with use of a questionnaire that included questions about daily consumption of tap water, bottled water, 21 food products and dishes. Iron concentration in food and drinking water was determined according to the reference tables and laboratory findings. Results: The harmless levels of AADD of iron for adolescent health (about 17 mg/day) were stated. An occurrence frequency of iron consumption deficiency was equal to 28.08 (boys) and 58,54 (girls) cases per 100 adolescents. Conclusions: Against iron excess in tap water according to the organoleptic limiting sign of iron content harmfulness (up to 2.7 MPC), iron consumption deficiency is formed by lower intake of high bioavailable iron sources: eggs, fishes (girls), eggs, sausage products and animal meat (boys). © Northern State Medical University, Arkhangelsk.

Kotsiuba A.Y.,Vladivostok State Medical University
Morfologii{combining double inverted breve}a (Saint Petersburg, Russia) | Year: 2012

Immunocytochemical method was used to determine the distribution of neurons expressing heme oxygenase-2 (HO-2-positive neurons) in the nuclei of various parts of the brainstem of 16 male Wistar rats. The sizes of neurons and the optical density of the product of histochemical reaction in their cytoplasm were determined in the nuclei studied. HO-2-positive neurons, differing in shape, size and numbers, were identified in the nuclei of the medulla oblongata, pons and midbrain. HO-2-positive cells were found 3-5 times more frequently in the sensory nuclei as compared to the motor ones. At the same time, relatively large number of nuclei was detected, which contained either no or a few HO-2-positive neurons.

Kotsyuba A.E.,Vladivostok State Medical University | Chertok V.M.,Vladivostok State Medical University
Tsitologiya | Year: 2013

ChAT-positive neurons in the nuclei of the medulla oblongata of Wistar rats have been studied with the use of histochemical and immunohistochemical methods. We have found that the topography and the number of cholinergic neurons at the projection nuclei studied largely depend on the method of detection of these neurons. Histochemical method always revealed more neurons than immunohistochemistry. Such a feature of ChAT-positive neurons detection was clearly seen among the majority of nuclei in the medial region and in some of the nuclei in the lateral region of the medulla oblongata. The number of immunoreactive cells in the nuclei varied from 17 to 26 %, whereas the histochemical reaction determined 1.5 - 3 times more neurons in the same nuclei. ChAT-positive cells in the nuclei of the back seam were detected mainly by a histochemical method.

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