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Fadeev R.S.,Russian Academy of Sciences | Solovieva M.E.,Russian Academy of Sciences | Slyadovskiy D.A.,Nizhni Novgorod Lobachevsky National Research University | Zakharov S.G.,Vladimirsky Moscow Regional Research Clinical Institute MONIKI | And 4 more authors.
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology | Year: 2015

One of the major causes of low efficiency of the therapy for acute myeloid leukemia is drug resistance of leukemic cells. Microenvironment plays a key role in formation of the phenotype of leukemic cell drug resistance. Investigation of the mechanisms of microenvironment-mediated drug resistance is important to identify novel pharmacological targets for the acute myeloid leukemia therapy. We studied the role of cell aggregation in the drug resistance of leukemic cells. We showed the increased resistance of acute myeloid leukemia cells THP-1 to bortezomib, doxorubicin and fludarabine in multicellular aggregates. In the multicellular aggregates of THP-1 with the higher drug resistance, cell proliferation activity did not change, while the intracellular level of anti-apoptotic protein Bcl-2 increased. Inhibition of the aggregation of THP-1 cells prevented drug resistance. This work demonstrates the involvement of cell aggregation in the formation of drug resistance phenotype in leukemic cells. © 2015, Pleiades Publishing, Ltd.

Rogatkin D.A.,Vladimirsky Moscow Regional Research Clinical Institute MONIKI | Dunaev A.V.,Orel State University | Lapaeva L.G.,Vladimirsky Moscow Regional Research Clinical Institute MONIKI
Biomedical Engineering | Year: 2010

The first steps in systematization and analysis of the most fundamental and specific metrological terms, concepts, and definitions applicable to noninvasive medical spectrophotometry (NMS) are discussed. An operational approach to the metrology for the purpose of creation and development of fully functional metrological support of NMS is suggested. Some key properties and aspects of optical in vivo measurements in NMS are discussed. Fabrication of simulation measures and introduction to NMS theory and practice of the notion of diagnostic volume of biological tissues are established as well. © 2010 Springer Science+Business Media, Inc.

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