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Indianapolis, IN, United States

Shannon H.E.,Herman lls Center For Pediatric Research | Fishel M.L.,Herman lls Center For Pediatric Research | Fishel M.L.,Indiana University | Xie J.,Herman lls Center For Pediatric Research | And 15 more authors.
Pancreas | Year: 2015

Objectives: The purpose of the present study was to develop and validate noninvasive bioluminescence imaging methods for differentially monitoring primary and abdominal metastatic tumor growth in mouse orthotopic models of pancreatic cancer.Methods: A semiautomated maximum entropy segmentation method was implemented for the primary tumor region of interest, and a rulebased method for manually drawing a region of interest for the abdominal metastatic regionwas developed formonitoring tumor growth in orthotopic models of pancreatic cancer. The 2 region-of-interest methods were validated by having 2 observers independently segment Panc-1 tumors, and the results were compared with the number of mesenteric lymph node nodules and histopathologic assessment of liver metastases. The findings were extended to orthotopic tumors of the more metastatic MIA PaCa-2 and AsPC-1 cellswhere separate groups of animals were implanted with different numbers of cells.Results: The results demonstrated that the segmentation methods were highly reliable, reproducible, and robust and allowed statistically significant discrimination in the growth rates of primary and abdominal metastatic tumors of different cell lines implanted with different numbers of cells.Conclusions: The present results demonstrate that primary tumors and abdominal metastatic foci in orthotopic pancreatic cancer models can be reliably quantified separately and noninvasively over time with bioluminescence imaging. © 2014 Lippincott Williams & Wilkins.

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