ICAR Vivekananda Institute of Hill Agriculture

Almora, India

ICAR Vivekananda Institute of Hill Agriculture

Almora, India
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Sood S.,ICAR Vivekananda Institute of Hill Agriculture | Gupta A.,ICAR Indian Institute of Wheat and Barley Research | Arun Kumar R.,ICAR Sugarcane Breeding Institute | Kant L.,ICAR Vivekananda Institute of Hill Agriculture | Bisht J.K.,ICAR Vivekananda Institute of Hill Agriculture
Electronic Journal of Plant Breeding | Year: 2016

White grained finger millet genotypes has become a thrust area in finger millet breeding due to increased demand of non-glutinous food products and lesser acceptability of brown grained finger millet. Sixteen white grain finger millet lines were developed by crossing extra early maturing brown grained finger millet genotypes including adapted varieties with late maturing white grained finger millet genotypes. The quantitative data of 16 lines along with parents were subjected to multivariate analysis. A wide range of variation was observed for all the studied traits. The parental lines of brown and white grained finger millet genotypes exhibited extreme values for grain yield and days to maturity whereas, the developed white grained genotypes showed moderate values. Projections of genotypes in PCA biplot showed close association of newly developed white grain genotypes VL 427, VL 360, VR 485, VR 443, VL 366, VR 425B, VR 425A and VL 356 with VL 201 (brown type) because of earliness and high yield potential. The cluster analysis further indicated that white grained genotypes from second cluster are probable candidates for further testing and release, and further refinement in breeding strategy by hybridizing white genotypes from second cluster with brown genotype in third cluster for incorporating earliness and high yield.


Sood S.,ICAR Vivekananda Institute of Hill Agriculture | Patro T.S.S.K.,Agricultural Research Station | Karad S.,Zonal Agricultural Research Station
Electronic Journal of Plant Breeding | Year: 2016

A study of phenotypic stability of 30 finger millet genotypes was conducted to assess genotype-environment interaction (GEI) and identify stable finger millet (Eleusine coracana (L.) Gaertn. subsp. coracana) genotypes for grain yield across four diverse locations in India. Genotypes, environments main effects and GEI were significant at P < 0.01. Different stability measures identified different wide adaptable genotypes. The parameters Wi2, σi2, Si(1) and Si(2) identified TNEC 1234, GPU 67, KMR 344, DHFMV 10-2-1, GPU 92 and VL 352 as stable genotypes, similarly ASV identified PPR 1044, PPR 1040, BR 45, GPU 91, VL 384 and DHFMV 78-3-1 while GPU 67, VL 376, DHFMV 10-2-1, GPU 92, VR 990 and GPU 91 were top six stable genotypes according to Ysi. The rank correlation matrix identified only two important stability measures (Ysi and YSI) in the identification of stable genotypes without compromise for grain yield. Among these two, Ysi was found to be the better choice for screening of genotypes for both yield and stability.


Sood S.,ICAR Vivekananda Institute of Hill Agriculture | Patro T.S.S.K.,Agricultural Research Station | Karad S.,Zonal Agricultural Research Station
Indian Journal of Genetics and Plant Breeding | Year: 2016

The present study aimed at deciphering the stability and patterns of genotype × environment interaction (GEI) in finger millet genotypes tested in All India Coordinated Trials using GGE biplot technique. The combined ANOVA for grain yield of five finger millet cultivars at four environments showed that environments (E), genotypes (G) and GEI were highly significant (P<0.01). GGE biplot grouped the four environments into two mega environments with GPU 67 and VR 990 as winning genotypes. VL 376 was found to be an ideal genotype in terms of high yield and stability followed by VR 990 as desirable genotype. © 2016, Indian Society of Genetics and Plant Breeding. All rights reserved.


Avupati R.N.S.S.,Govind Ballabh Pant University of Agriculture & Technology | Avupati R.N.S.S.,ICAR Vivekananda Institute of Hill Agriculture | Khan M.S.,Govind Ballabh Pant University of Agriculture & Technology | Johnson S.,ICAR Vivekananda Institute of Hill Agriculture | And 2 more authors.
Applied Soil Ecology | Year: 2017

Chitinolytic Bacillus and associated chitinases have prospective implication in both industry and biological control of agricultural pests. A total of 83 chitinolytic Bacillus strains were isolated from native soils of Uttarakhand state, north western Indian Himalayas. A multiphasic characterization of the collection showed a vast diversity in bacterial kinetics (growth and enzyme production), pH stability in ten isolates, thermal-stability in 23 isolates and psychrotolerance in three isolates. The collection was characterized by chief existence of 148 kDa (65 isolates) and 152 kDa (7 isolates) chitinases, the high molecular weight enzymes. Partial sequencing of 16S rRNA and chi genes showed Paenibacillus species are the major chitinase producers of the region. In addition, licheniformis and circulans group chitinases were also found to be associated with the collection. The molecular variation in sequences revealed existence of different Paenibacillus species and especially a special evolutionary status of chitinases in phylogeny which is also evidenced by associated high molecular weight chitinases. The bioactivity of isolates against insect pests, Pieris brassicae and Helicoverpa armigera showed that the isolates were not lethal except at very high concentrations (108 cfu/ml) that to in only 9 isolates. However, they are primarily involved in growth reduction which led to identification of three prominent isolates (UKCH27, UKCH29 and UKCH77) that caused a significant larval weight reduction at lowest concentration tested (102 cfu/ml). Further testing of synergism between Cry toxins of B. thuringiensis strain HD 1 and these isolates showed near cent percent mortality of test insects at LC30 concentrations. © 2017 Elsevier B.V.


Mishra K.K.,ICAR Vivekananda Institute of Hill Agriculture | Pal R.S.,ICAR Vivekananda Institute of Hill Agriculture | Bhatt J.C.,ICAR Vivekananda Institute of Hill Agriculture
Emirates Journal of Food and Agriculture | Year: 2015

Total phenolics, condensed tannins, ascorbic acid, total antioxidant activity, reducing power, ferric reducing antioxidant power, radical scavenging activity (RSA) on DPPH&ABTS and metal chelating activity of methanolic and aqueous extract from cap and stipe of Lentinula edodes have been evaluated. Different extracts contained 2.40-5.60 mg gallic acid equivalent of phenolics, 1.23-3.26 mg catechins equivalent of condensed tannins, and 2.31-11.96 mg ascorbic acid per gram of extract. Aqueous extract from cap contained higher phenolics, condensed tannins, ascorbic acid, RSA on DPPH and ABTS, reducing power, and metal chelating activity. We found that this mushroom species present antioxidant potential especially higher for cap indicating that the cap is the material that most contributes to the antioxidant activity.


Mishra K.K.,ICAR Vivekananda Institute of Hill Agriculture | Pal R.S.,ICAR Vivekananda Institute of Hill Agriculture | Mishra P.K.,ICAR Vivekananda Institute of Hill Agriculture | Bhatt J.C.,ICAR Vivekananda Institute of Hill Agriculture
Asian Journal of Chemistry | Year: 2016

The aim of the present investigation was to determine the antioxidant activities and mineral composition of oyster mushrooms (Pleurotus sajor-caju) as influences by different drying methods. Different dried oyster mushroom samples had 27.3-31.4 mg GAE/g dry wt. of phenolics, 2.19-53.74 % of radical scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 16.36-94.88 % of radical scavenging activity on 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 61.77-265.08 μM FeSO4 equivalent of ferric reducing antioxidant power (FRAP), 144.03-372.05 μM Trolox equivalent of total antioxidant activity, 0.07-0.30 of reducing power and 23.13-78.12 % of metal chelating activity. Higher amount of nitrogen (4.84 %), potassium (5.07 %), zinc (0.093 mg/g), copper (0.010 mg/g), iron (0.168 mg/g) and manganese content (0.035 mg/g) was found in solar dried oyster mushroom samples. Principal component analysis revealed that radical scavenging activity on DPPH and ABTS are having positive correlation with solar dried P. sajor-caju. We found that solar dried oyster mushrooms are rich in different antioxidant activities and minerals composition. © 2016, Chemical Publishing Co. All rights reserved.


Pal R.S.,ICAR Vivekananda Institute of Hill Agriculture | Bhartiya A.,ICAR Vivekananda Institute of Hill Agriculture | ArunKumar R.,ICAR Vivekananda Institute of Hill Agriculture | Kant L.,ICAR Vivekananda Institute of Hill Agriculture | And 2 more authors.
Journal of Food Science and Technology | Year: 2016

The changes in chemical composition, antioxidant activity and minerals content of horse gram seed after dehulling and germination of 12 advance lines were investigated. Dehulled samples had a higher protein content compared with the raw and germinated. Total soluble sugars (TSS) content increased significantly (p ≤ 0.05) after dehulling (29.31 %) and germination (98.73 %) whereas, the total lipids increased (10.98 %) significantly (p ≤ 0.05) after dehulling and decreased (36.41 %) significantly (p ≤ 0.05) after germination. Dehulling and germination significantly decreased the amount of phytic acid (PA), tannin (TN) and oxalic acid (OA). Trypsin inhibitor units decreased (26.79 %) significantly (p ≤ 0.05) after germination. The minerals (Ca, Fe and Cu) composition of the germinated horsegram flour samples was significantly higher than the raw and dehulled flour. The functional properties of flours were studied and found that the bulk density (11.85 %) and oil absorption capacity (18.92 %) significantly increased after germination. Raw samples followed by germinated samples showed the highest concentrations of phytochemicals responsible for the antioxidant activity and also the antioxidant capacities. principal component analysis revealed that in case of dehulled samples; TN, polyphenols, DPPH and ABTS radical inhibition, TSS, total antioxidant, OA, protein, FRAP value, Ca and Zn had positive correlation among themselves while in case of germinated samples, protein, oil absorption capacity, FRAP value, OA, total flavonoids, DPPH radical inhibition, Ca and Cu had positive correlation among themselves. Present study suggest that germination combined with dehulling process improved quality of horsegram by enhancing the nutritive value and reducing the antinutrients. © 2015, Association of Food Scientists & Technologists (India).


Ghosh B.N.,ICAR Central Soil and Water Conservation Research and Training Institute | Meena V.S.,ICAR Vivekananda Institute of Hill Agriculture | Alam N.M.,ICAR Central Soil and Water Conservation Research and Training Institute | Dogra P.,ICAR Central Soil and Water Conservation Research and Training Institute | And 3 more authors.
Agriculture, Ecosystems and Environment | Year: 2016

The carbon management index (CMI) and labile organic carbon (LOC) pools are postulated as very sensitive indicators of changes in soil organic carbon (SOC) due to land degradation within a short time in response to management practices. To test this hypothesis, we investigated LOC and CMI under a field experiment (2007-2013) in relation to runoff, soil loss, maize and wheat yields on a 2% (1.15°) land slope of the Indian Himalayas. In this study, the impacts of several resource conservation practices, including different combinations of vegetative barriers (VB), minimum tillage (MT), different organic amendments (OA) and weed mulch, were evaluated. Results revealed that the plots under MT + OA with three applications of weed mulch had more SOC, macroaggregate-associated C concentrations and macroaggregates than conventional tillage (CT) + NPK with chemical weed control. Carbon management index varied from 47 to 59 and 42 to 55% with different conservation practices at depths of 0-5 and 5-15. cm depths, respectively. Incorporation of weed mulch along with application of OM, MT and VB (by Palmarosa) under MT improved CMI by 19.7 and 24.2% compared to CT plots with VB (by Panicum) and inorganic NPK at depths of 0-5 and 5-15. cm, respectively. Significant positive correlations were observed between CMI and maize yield (r= 0.948; n= 24; P<. 0.01), CMI and wheat yield (r= 0.872; n= 24; P< 0.01) and CMI and wheat equivalent yield (r= 0.906; n= 24; P< 0.01). However, significant negative correlations were obtained for CMI and runoff (r= -0.701; n= 20; P< 0.01) and CMI and soil loss (r= -0.768; n= 20; P< 0.01). Results established that Palmarosa as VB along with OA plus weed-mulch under MT was the best management practice for decreasing runoff and soil loss and increasing system productivity on a 2% slope in the region. The single value CMI was strongly positively correlated with crop productivity and negatively correlated with soil loss. Hence, this single value CMI could potentially be used for assessment of soil degradation elsewhere. © 2015 Elsevier B.V.


PubMed | ICAR Vivekananda Institute of Hill Agriculture
Type: Journal Article | Journal: Journal of food science and technology | Year: 2016

The changes in chemical composition, antioxidant activity and minerals content of horse gram seed after dehulling and germination of 12 advance lines were investigated. Dehulled samples had a higher protein content compared with the raw and germinated. Total soluble sugars (TSS) content increased significantly (p0.05) after dehulling (29.31%) and germination (98.73%) whereas, the total lipids increased (10.98%) significantly (p0.05) after dehulling and decreased (36.41%) significantly (p0.05) after germination. Dehulling and germination significantly decreased the amount of phytic acid (PA), tannin (TN) and oxalic acid (OA). Trypsin inhibitor units decreased (26.79%) significantly (p0.05) after germination. The minerals (Ca, Fe and Cu) composition of the germinated horsegram flour samples was significantly higher than the raw and dehulled flour. The functional properties of flours were studied and found that the bulk density (11.85%) and oil absorption capacity (18.92%) significantly increased after germination. Raw samples followed by germinated samples showed the highest concentrations of phytochemicals responsible for the antioxidant activity and also the antioxidant capacities. principal component analysis revealed that in case of dehulled samples; TN, polyphenols, DPPH and ABTS radical inhibition, TSS, total antioxidant, OA, protein, FRAP value, Ca and Zn had positive correlation among themselves while in case of germinated samples, protein, oil absorption capacity, FRAP value, OA, total flavonoids, DPPH radical inhibition, Ca and Cu had positive correlation among themselves. Present study suggest that germination combined with dehulling process improved quality of horsegram by enhancing the nutritive value and reducing the antinutrients.


Saha M.,Banaras Hindu University | Maurya B.R.,Banaras Hindu University | Meena V.S.,Banaras Hindu University | Meena V.S.,ICAR Vivekananda Institute of Hill Agriculture | And 2 more authors.
Biocatalysis and Agricultural Biotechnology | Year: 2016

The present investigation comprises a total of fifty potassium solubilizing bacterial (KSB) strains which were isolated from Oryza sativa, Musa paradisiaca, Zea mays, Sorghum bicolor and Triticum aestivum L. These strains were evaluated for their ability to solubilize the fixed K from waste biotite (WB). On the basis of K-solublization, the seven most efficient KSB strains were evaluated for K-solublizing dynamics from the WB at 7, 14 and 21 DAI (days after incubation) on MAMs (Modified Solid Aleksandrov Medium). Further, these screened seven KSB strains were used for their morphological, physiological and molecular chacterization. The KSB strains Bacillus licheniformis BHU18 and Pseudomonas azotoformans BHU21 showed significantly higher K-solublization 7.22 and 6.03 μg mL-1 at 30 °C and pH 7.0, respectively. A significantely higher zone of solubilization significantly higher was recorded with Pseudomonas azotoformans BHU21 (3.61 cm). Bacillus licheniformis BHU18 produced significantly higher (~23 μg mL-1) concentrations of indole-3-acetic acid. The diversity of KSB as bioinoculants to release potassium provides a win-win situation. Therefore, it is crucial to adopt efficient KSB strain interventions for the judicious use of chemical and biological resources for maximizing food production while reducing pollution and rejuvenating degraded land for agricultural benefit. © 2016 Elsevier Ltd.

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