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Karunakara C.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5 | Aparna U.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5 | Chandregowda V.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5 | Reddy C.G.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5
Analytical Sciences | Year: 2012

A simple and rapid reverse-phase high-performance liquid chromatographic (HPLC) method for the simultaneous separation and determination of erlotinib and its process-related impurities in bulk drugs has been developed. Five process-related impurities of erlotinib have been separated on an Inerstsil ODS-3V (C18) column and detected at 254 nm using a photo diode array (PDA). This HPLC method was successfully applied to the analysis of erlotinib bulk drug. The recoveries of erlotinib and process-related impurities were in the range of 92.86 - 106.23%, and found to be specific, precise and reliable for the determination of unreacted raw materials, intermediates in the reaction mixtures and bulk drugs. © 2012 The Japan Society for Analytical Chemistry.


Chandrashekara K.A.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5 | Hegde R.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5 | Kush A.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5 | Reddy C.G.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5
International Journal of ChemTech Research | Year: 2014

Azadirachtin (AZA) and β-asarone (BAS) which are lipophilic in nature were made into water soluble formulation by encapsulating them in to β- methyl cyclodextrin (BMCD). This formulation was found to effective against plant feeding insects and plant pathogenic fungi. A simple and fast HPLC method was developed for simultaneous estimation of azadirachtin and β-asarone in the formulation. AZA and BAS were separated on Nova Pak (150 × 0.39 mm, 4 μm) column using water and acetonitrile as mobile phase by gradient elution. Peaks were monitored at 210 nm using photo diode array (PDA) detector. The developed method was validated for linearity, accuracy, precision and sensitivity. Linear regression analysis revealed an excellent correlation between peak responses and concentrations (R2 values of 0.999) for the AZA and BAS. The developed HPLC method was applied for estimation of AZA and BAS in multi component water soluble biopesticide formulation. The recoveries of AZA and BAS were in the range of 94.37-99.98% and 99.26-100.08% respectively, and precision values were less than 4.5% © 2014, Sphinx Knowledge House. All rights reserved.


Karunakara C.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5 | Aparna U.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5 | Reddy C.G.,Vittal Mallya Scientific Research Foundation VMSRF and 94 3 and 94 5
Journal of Liquid Chromatography and Related Technologies | Year: 2015

γ A simple, fast, and precise high performance liquid chromatographic method was developed for the estimation of seven intermediates formed during the synthesis of gefitinib, an anticancer drug by a patent noninfringing route. Also, one degradation product (gefitinib N-oxide) formed during oxidative stress were analyzed and identified. All these compounds were separated on Inertsil ODS-3 V (250 × 4.6 mm, 5 m) column using isocratic mobile phase consisting of ammonium acetate (0.05 M)-acetonitrile-methanol (70:25:5, v/v/v) (pH 4.1) at a flow rate of 1 mL/min and detected at 260 nm by a photo diode array (PDA) detector. The developed method was validated for accuracy, precision, linearity, sensitivity, and ruggedness. Linear regression analysis revealed an excellent correlation between peak responses and concentrations (R2 values > 0.995) for the drug and impurities. The proposed HPLC method was applied for estimation of process-related intermediates/impurities in gefitinib bulk drug; the recoveries were in the range 91.09-103.07%. The method was found to be specific, precise, and reliable for estimation of process-related intermediates/impurities and degradation impurities in gefitinib bulk drug. The highlight of this technique is that all the intermediates/impurities of gefitinib could be analyzed with baseline separation and reduced run time. © 2015 Taylor & Francis Group, LLC.

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